tera Five signs that Colorado’s literary scene is on the rise By www.denverpost.com Published On :: Fri, 13 Sep 2024 12:00:05 +0000 “Denver’s a deliciously unpretentious place in which to engage in art making,” said Lighthouse Writers program director Andrea Dupree. Full Article Arts Books Education Entertainment Latest Headlines News The Know Things To Do book books reading Tattered Cover Book Store
tera Can AI make video games more immersive? Some studios turn to AI-fueled NPCs for more interaction By www.denverpost.com Published On :: Sat, 19 Oct 2024 12:00:16 +0000 Generative AI could also provide more opportunities for players to go off-script and create their own stories if designers can craft environments that feel more alive and can react to players’ choices in real-time. Full Article Business Latest Headlines Technology artificial intelligence development More Business News role-playing games technology video games
tera Endangered Species Skinks & Cahows Interact By bernews.com Published On :: Sat, 21 May 2022 23:47:27 +0000 As the world marks ‘Endangered Species Day,’ Nonsuch Expeditions is highlighting two of Bermuda’s IUCN Red Listed species — the cahow and skink — noting that the two critically endangered species are interacting on Nonsuch Island where they can “thrive without the threat of introduced predators.” Senior Terrestrial Conservation Officer Jeremy Madeiros, head of the […] Full Article All Environment News #Animal #BermudaCahow #BermudaSkink #Birds #NonsuchIsland
tera BSX Rings The Bell For Financial Literacy By bernews.com Published On :: Sun, 13 Oct 2024 13:06:23 +0000 The Bermuda Stock Exchange [BSX] hosted a ceremonial ‘Ring the Bell for Financial Literacy’ event as part of the World Federation of Exchanges’ [WFE] support for World Investor Week. A spokesperson said, “The Bermuda Stock Exchange [BSX], a wholly owned subsidiary of Miami International Holdings, Inc., hosted a ceremonial ‘Ring the Bell for Financial Literacy’ […] Full Article All Business #BermudaBusiness #BSX
tera Interactive Village Gathering On October 12 By bernews.com Published On :: Wed, 09 Oct 2024 12:47:53 +0000 An Interactive Village Gathering will be held on Saturday [Oct 12] from 4.00pm to 5.30pm at the Victor Scott School celebrating the 75th anniversary of free primary education in Bermuda. A spokesperson said, “Reminded of the African proverb ‘it takes a village to raise a child,’ a collaborative group of community stakeholders have come together […] Full Article All #BermudianChildren #Education
tera Veterans To Meet Again In Gold Cup Final By bernews.com Published On :: Sun, 03 Nov 2024 01:04:55 +0000 Veteran match racers Eric Monnin of Switzerland and Johnie Berntsson of Sweden are set to face off in the Bermuda Gold Cup final, ten years after their last Gold Cup final match-up. A spokesperson said, “Match racing veterans Eric Monnin from Switzerland [Capvis Swiss Match Racing Team] and Sweden’s Johnie Berntsson [Berntsson Sailing Team] will […] Full Article All Sports #BermudaSailing #SailingGoldCup
tera Author Clara Fay Wins Three Literary Awards By bernews.com Published On :: Mon, 04 Dec 2023 14:08:34 +0000 [Written by Stephen Wright] Author Clara Fay’s debut novel, Mark of a Crescent Moon, has earned rave reviews from critics and won three literary awards. The novel, described as a “compelling tale of intrigue, destiny, and the surprising intersection of an artistic and spiritual journey” is available at the The Bookmart, Bermuda Bookstore in Hamilton, […] Full Article All Entertainment News #BermudaBooks #GoodNews #StephenWrightReports
tera 2023 Bermuda Literary Awards Winners By bernews.com Published On :: Tue, 27 Feb 2024 20:51:23 +0000 The winners of the 2023 Bermuda Literary Awards have been announced, with seven winners across the same number of categories. A Government spokesperson said, “Minister of Tourism, Culture and Sport Senator Owen Darrell announced the 2023 Bermuda Literary Awards winners. Dr. Angela Barry, Elizabeth J. Jones, Junior Minister of Tourism, Culture and Sport Kim Swan, […] Full Article All Entertainment News #AwardWinners #BermudaBooks #GoodNews
tera Column: Myron Publishes On Financial Literacy By bernews.com Published On :: Sat, 25 May 2024 11:36:47 +0000 [Written by Martha Harris Myron] It has been my long-term experience in my former Bermudian/international professional finance practice working with our community that still, there is little correlated, current, easy-to-understand financial information specifically focused on our uniquely sophisticated Bermuda economic environment. Bermuda’s First Financial Literacy Primer was written by me for you, all Bermuda islanders […] Full Article All #BermudaBooks #Finance
tera ‘Darien Shockra’ Wins Literary Titan Gold Award By bernews.com Published On :: Thu, 19 Sep 2024 15:21:54 +0000 The novella Darien Shockra and The Bermuda Triangle – Part 1 by Bermudian author David Kneisler and Stefan Lukjanczuk has won the Literary Titan Gold Book Award. A spokesperson said, “Kinetikz Publishing is thrilled to announce that the highly anticipated urban fantasy novel, “Darien Shockra and The Bermuda Triangle – Part 1,” written by David […] Full Article All Entertainment #AwardWinners #BermudaBooks #BermudaTriangle #GoodNews
tera Mapping Mary Prince Interactive Visualization By bernews.com Published On :: Sat, 26 Oct 2024 10:03:48 +0000 Mapping Black London — which is led by Northeastern University and aims to “visualise the multi-racial foundations and histories of London and the UK” — has created an interactive map chronicling the life of Bermudian Mary Prince. The organization said, “This exhibit traces the life of Mary Prince through the locations she described in her […] Full Article All History #BermudaHistory #MaryPrince
tera Veterans Day 2024 By www.google.com Published On :: Date: November 11, 2024 Location: Puerto Rico, U.S. Virgin Islands, United States Tags: Full Article
tera alterations and repairs By www.oglaf.com Published On :: Sun, 31 Jan 2021 00:00:00 +0000 Full Article
tera McFib: An eLearning Interaction Pattern By www.bill-wilson.net Published On :: Wed, 15 Jul 2020 12:30:00 +0000 I'm very interested in eLearning designs that try to entwine content and exercise so thoroughly that the learner almost constructs their instruction – even if... Full Article eLearning attention elearning demo generation intrinsic programming programmed instruction
tera Drag/Drop Interactions in eLearning Design By www.bill-wilson.net Published On :: Sun, 26 Jul 2020 14:46:46 +0000 Sometimes, I feel like eLearning drag/drop interactions get a bad rap. Maybe that's because Serious Instructional Designers seem fairly dismissive about them in casual conversation?... Full Article eLearning accessibility AGES model cognitive load theory drag-drop elearning demo interactive learning design self determination theory
tera Biblical and Budai Taiwanese: vernacular, literary; oral, written By languagelog.ldc.upenn.edu Published On :: Sun, 10 Nov 2024 12:21:52 +0000 [This is a guest post by Denis Mair] Cai Xutie was a Taiwanese woman who ran a family farm with her husband in a village near Jiayi in central Taiwan. She was a rice farmer and had never attended a public school. After her husband died in middle age, she sold some of the land, […] Full Article Language and entertainment Language and religion Literacy Topolects
tera veteran and vet (noun) By separatedbyacommonlanguage.blogspot.com Published On :: Sun, 19 Feb 2023 18:49:00 +0000 More than once, I think, veteran or (the noun vet) has been nominated for US>UK Word of the Year. Dru, who nominated it for 2022, felt that it was appearing more often in UK contexts:The word I’d propose is ‘veteran’ in the US sense of a former soldier. Some may dispute this as a word for this year as many of us have long been aware of it as an American expression, but since the summer of this year, I’ve increasingly heard it used on the BBC and elsewhere to meaning a former member of the UK armed services.In the UK hitherto, it has just meant ‘old’, possibly slightly distinguished and used of cars etc.The US abbreviation ‘vet’ causes confusion here as ‘vet’ means a doctor for animals, short for veterinary surgeon.I considered making it the WotY, but it didn't feel 2022-ish enough. (You'll see why below.) But I put it on my to-be-blogged-about-sooner-rather-than-later list, and here we are! If you don't want to see all (BrE) my workings, scroll down to the TL;DR version.From: "7 things to know about being a military veterinarian"The ex-soldier sense of veteran wasn't made up by Americans. Since the 1500s, veteran has been an English noun referring first to someone with "long experience in military service or warfare" (Oxford English Dictionary sense 1a) or "an ex-member of the armed forces" (sense 1b). Note the difference there: in the 1a meaning, the person is still probably serving, whereas in the 1b meaning they're retired from service. That second (1b) meaning, the OED notes, is "now chiefly North American," though there are UK examples peppered through their timeline of quotations. In BrE it is still used for sense 1a, to refer to old-but-still-going things or people. It's sometimes used like that in AmE too, often in relation to theat{er/re}, as in a veteran of stage and screen. The usage that Dru mentioned, veteran car, is particularly BrE. In AmE, you could call such a thing a vintage car (as in BrE too) or an antique car, as shown here in the GloWbE corpus: It's tricky to investigate whether the ex-soldier meaning of veteran is going up in BrE usage because how much we talk about veterans varies a lot according to what's going on in the world. But to have a little look-see, I searched for the phrase "war veteran(s)" in Hansard, the record of the UK Parliament. There is almost no usage of the phrase before 1990, then a lot more in 2000–2009. Now, maybe some of these are in sense 1a, the 'been serving for a long time' sense. But a peek at the data shows that most of the 2000s examples relate to compensation for Gulf War veterans, so it does seem to be more the ex-soldier meaning. Note that [more AmE] WWI/WWII veterans are usually called First/Second World War veterans in BrE, and there was the Falklands War after that, so it's not that there were no "war veterans" before the 1990s. A different tool, Hansard at Huddersfield, takes us up to 2021, and there we can see that this use of veteran appears to have stabilized, rather than continuing to increase. But in Covid Times, it's likely that there was just less debate about ex-servicepeople in Parliament—so we can't make too much of that stability. It could be increasing in comparison to other ways of talking about ex-servicepeople. What about vet?I've written about vet before—in fact it was my 2008 UK>US Word of the Year. But in that case it was a verb (as in to vet a candidate). Now I want to just look at the noun—or nouns.Vet can be short for (more AmE) veterinarian/(BrE) veterinary surgeon. You take your pet to the vet. It rhymes and everything. Let's call that vet1. The OED has examples going back to 1862, and marks it as "chiefly British", which, as we're going to see, might not be the best way to describe it. In AmE since the 1840s, vet has been used as a shortened form of veteran. Let's call that vet2.In AmE, where both are used, context is usually enough to tell the difference between vet1 and vet2. You take your dog to the vet1. People study at vet1 school. But a Vietnam vet is probably a vet2 and not a Vietnamese vet1. Both vets are well-used in AmE. I used english-corpora.org to take a 100-sentence sample of the noun vet from the Corpus of Contemporary American English. Of the 100, 57 definitely referred to the animal doctor, 23 referred to former soldiers, 3 referred stage or other veterans, and 6 were neither of these nouns (1 verb, some acronyms, a typo, and a Dutch word). That leaves 11 where I couldn't tell in the very brief window of text which vet it was; it referred to a person who'd been introduced earlier in the text. Had I had the full text, I assume there would be close to zero ambiguous cases—but even with a very short window of context, it was usually easy to tell. (For some examples, see below. Click to enlarge.) In any case, note that the majority refer to the animal doctor. I had a quick peek in the Corpus of Historical American English, and the phrase "to the vet" (as in I took my dog...) is there since the 1940s, increasing in use each decade. While the singular was usually the animal doctor in AmE, in the plural, vets, it's more likely to refer to former soldiers, since they are more often discussed as a class than veterinarians are. So, as is often the case for homonyms, context usually tells us which thing we mean.Is the use of vet2 increasing in BrE? Well, probably some, but it's harder to find good evidence for it. There are scattered uses of war vets in Hansard since the 1960s, but it's probably too new and informal to be used in parliamentary talk. When I was researching it as a possible Word of the Year, I looked at samples from the News on the Web corpus, and found 5 examples (of 100 vet) in 2011 and seven in 2022 (the highest years were 2019 at 11 and 2020 at 20, but there were only 3 in 2021). My small sample size could have skewed things (but it was as much as I could give time for). A lot of the UK examples I looked at were about American vets, in which case the UK news source could have been quoting an American person or possibly publishing text from a wire service, possibly originally written by an AmE speaker. So, as I say, it's not simple to spot the truly BrE usage. TL;DR versionThe full form veteran (in the ex-soldier sense) is definitely used in the UK these days. Though it is now perceived as an Americanism, it originally came from Britain, and it probably never entirely went away there. Vet as an abbreviation of veteran, originates in AmE, and is still used there. Vet as an abbreviation for veterinarian/veterinary surgeon is originally BrE, but has been well used in AmE for a long time (or at least, throughout my lifetime!). The ambiguity this creates hasn't been a huge problem. No one's mistakenly taking their dog to the VFW. Full Article clipping occupations
tera Teraco Taps Absa for $442 Million Loan for AI-Ready Facility By biztoc.com Published On :: Wed, 13 Nov 2024 07:12:58 GMT In This Article: (Bloomberg) -- Teraco Data Environments Ltd. hired Absa Group Ltd. to syndicate an 8 billion rand ($442 million) loan as the company gears up for a new facility to meet rising demand from artificial intelligence applications. Most Read from Bloomberg The facility will push… Full Article
tera Do Natural Organic Positions and AdWord Postions Work Interactively? By linkworxseo.wordpress.com Published On :: Mon, 07 Jan 2013 02:20:01 +0000 How Organic Placement is Affected Some people seem to be hell-bent on believing that it matters. There is no problem with believing it does have a direct effect on organic placement, but the fact of the matter is; it does not. Although you are entitled to believe what you want to believe, but knowing whether […] Full Article LINK BUILDING PAY PER CLICK (PPC) adwords natural natural-organic-placement organic organic-positions pay-per-click ppc
tera Makes You Want To Throw The Book At Him. Literally. By clientsfromhell.tumblr.com Published On :: Tue, 04 Jan 2022 09:00:17 -0800 My boss is a seventy-something-year-old man with barely a clue on how to get a computer to boot. He gave me an old book and wanted me to convert it into an ebook to sell. And mind, this was a thick, large-format book chock-full of maps and other illustrations with tiny blackletter script. Me: “Sure, I can do it, but I’d have to scan it in a massive resolution so the detail isn’t lost. The final file would be massive; it wouldn’t be practical to download it, and a normal ebook reader wouldn’t be able to display it correctly.” Boss: “So, we’d have to make it less detailed.” Me: “How do you mean?” Boss: “It wouldn’t be possible with the illustrations; you’ll just have to make the writing bigger on all the pages.” Me: “…” Boss: “As for the pages with only text on them, you will just convert them into a Word document.” Me: “That’s not how that works.” Boss: “Why not?” Me: “It’s just straight-up not possible, at least not with the software we have.” Boss: “Can you do it on the Internet?” Me: “No.” Boss: “How do you know?” Me: “I know.” Boss: “Show me.” I showed him that it’s not possible to convert a scanned book page into a text document on some random converter found on page one on Google. Boss: “Okay, so you will instead cut the text out in Photoshop, make it larger, and arrange it on a new Photoshop file the same size, with less of a rim around it so the number of pages doesn’t get much higher.” I flat-out refused, telling him it would be months of absolutely pointless work. He didn’t believe any of my claims, anyway, so I just converted the whole d*** thing into an ebook, which, in the end, was like 8GB in size. Since our server had 10TB, he also didn’t believe me when I tried to tell him that it was an absurdly massive file that few people would want to buy on that account. Ah, well. At least I didn’t have to rearrange like 300 pages of text. Full Article
tera Veterans Day observance to be held at the Princeton University Chapel By www.princeton.edu Published On :: Fri, 08 Nov 2024 10:59:36 -0500 The 9 a.m. service on Monday, Nov. 11, will also be livestreamed. Full Article
tera A new game teaches financial literacy and decision-making By www.pbs.org Published On :: Mon, 18 Jul 2022 16:07:03 +0000 How can you identify and overcome biases that hurt you financially? NOVA teamed up with Duke University’s Center for Advanced Hindsight to design the NOVA Financial Lab, a game that breaks down the behavioral science behind financial decision-making. Full Article
tera A Marine Corps veteran expresses concerns for the military in a 2nd Trump presidency By www.npr.org Published On :: Mon, 11 Nov 2024 12:07:44 -0500 Essayist Phil Klay says Trump tried to use the military to push his partisan agenda before, and may further erode norms around the military as he looks for those willing to "go with his whims." Full Article
tera Air Force veteran Anna Luna runs Charlie Crist By shark-tank.com Published On :: Tue, 18 Feb 2020 05:08:13 +0000 Air Force veteran and former Turning Point USA spokesperson Anna Paulina Luna has just announced she is running for the U.S. Congress against Democratic Congressman Charlie Crist in Florida’s 13th congressional district. The post Air Force veteran Anna Luna runs Charlie Crist appeared first on Shark Tank. Full Article Featured immigration islam
tera Gov. Ivey’s treatment of Bama veterans is a scandal By quinhillyer.com Published On :: Tue, 12 Nov 2024 02:55:07 +0000 (Nov. 9, to kick off Veterans Day weekend) MOBILE, Alabama — On this Veterans Day, Alabama is roiled by a doozy of a veterans-related controversy. Republican Gov. Kay Ivey last month invoked […] The post Gov. Ivey’s treatment of Bama veterans is a scandal appeared first on Quin Hillyer. Full Article Email Featured Gulf Coast Politics National Politics
tera “If the Democrats could fight wars as well as they execute Election Fraud, we would have obliterated all of our many enemies throughout the World, and would have nothing to worry about!” ~ Donald J. Trump By powderedwigsociety.com Published On :: Sun, 29 Aug 2021 14:34:49 +0000 Touché! .@POTUS45: "If the Democrats could fight wars as well as they execute Election Fraud, we would have obliterated all of our many enemies throughout the World, and would have nothing to worry about!" pic.twitter.com/GpMO46zrNQ — Natalie Harp (@NatalieJHarp) August […] The post “If the Democrats could fight wars as well as they execute Election Fraud, we would have obliterated all of our many enemies throughout the World, and would have nothing to worry about!” ~ Donald J. Trump appeared first on Powdered Wig Society. Full Article 2020 presidential election 2020 election fraud afghanistan donald trump
tera Biden’s Nitwit Dept. of Defense Uses Photo of Chinese Jet in Veterans Day Message By lidblog.com Published On :: Wed, 13 Nov 2024 02:27:52 +0000 Joe Biden’s stupid, corrupt regime can’t even get a simple Veterans Day message right. As the nation came together to celebrate those who served out country, Biden’s Department of Defense pushed out a social media message telling Americans “Veterans Day, honoring all who served.” While, that is a perfectly fine message, the DoD added a […] The post Biden’s Nitwit Dept. of Defense Uses Photo of Chinese Jet in Veterans Day Message appeared first on The Lid. Full Article China Military
tera Teachers can assess young students’ literacy skills and knowledge by encouraging them to produce books based on animal facts. By www.npr.org Published On :: Mon, 03 Jul 2023 09:49:56 EDT A new children's book transforms a sad, scared and anxious little boy into a superhero. The book is called "Cape," in honor of the bright-red cape the little boy wears and finds comfort in following the death of his father. "Cape" is Kevin Johnson's debut picture book, and it's vividly illustrated by artist Kitt Thomas. Full Article
tera Top Ducati executive explains how the Army helped him succeed and shares 2 traits that make veterans great hires By www.businessinsider.com Published On :: Tue, 12 Nov 2024 20:04:26 +0000 Ducati North America CEO Jason Chinnock enlisted in the US Army out of high school and served with the Third Armored Division in Desert Storm. Full Article Transportation Careers ducati veterans
tera Shared Reading in the Structured Literacy Era By www.readingrockets.org Published On :: Wed, 16 Nov 2022 13:46:38 EST Teacher question: Can you provide clarification on how to promote shared reading in the structured literacy era and how that differs from shared reading in the balanced literacy era. I would think a teacher could certainly initially read the text aloud to students to model fluency and expression, but then must ensure students can get the words off the page and reread by decoding the words, rather than parroting the teacher or memorizing the shared reading text that may be a rhyme/song that is catchy. Shanahan's response: Full Article
tera Print-to-Speech and Speech-to-Print: Mapping Early Literacy By www.readingrockets.org Published On :: Wed, 14 Dec 2022 11:00:57 EST The “Reading Peaceniks” The authors of this article are a group of researchers and practitioners who are looking to end the divisiveness of the “reading wars” — and help children learn to read and write with competence and pleasure. See the list of contributing editors as well as the 25 nationally recognized colleagues who support this effort. Full Article
tera More on Hanford: Phonics Reform and Literacy Levels By www.readingrockets.org Published On :: Mon, 06 Feb 2023 12:08:57 EST Recently, I posted commentary on Emily Hanford’s reporting and the critical response it received from some in the literacy community. I defended the major thrust of her work and called out criticisms I thought to be illogical, ill conceived, or ill intended — criticism more aimed at maintaining status quo than promoting literacy. Full Article
tera Interactive Media Strategies believes Microsoft could win big with Skype By communicationtransformation.blogspot.com Published On :: Fri, 13 May 2011 22:01:00 +0000 Interactive Media Strategies (IMS) is a market research firm that follows streaming media. They discuss the news of Microsoft’s $8.5 billion acquisition of Skype, and the prospects for Skype to emerge as a viable platform for business video communications. Their research compares usage levels for a range of social media and communications applications, including Skype, YouTube and Facebook. Here is a link to the video. They report that personal use of Facebook and YouTube is relatively high but that has not yet translated into comparable levels of corporate use. They believe this raises the question of whether YouTube and Facebook will be able to outgrow their focus on the consumer side of the business to compete in the enterprise market. Usage levels for Skype are significantly lower overall, but Skype’s penetration in business communications is higher than they see for other communications apps online among young users most likely to experiment with emerging technologies. IMS puts forth the opinion that if Microsoft could make it easier for technology laggards to embrace Skype - i.e. integrate Skype with the Microsoft Office suite - then Skype could achieve significant enterprise adoption. Full Article
tera INTERNal Stupidity: Pentagon Interns Post Pic of Chinese Plane on Veteran’s Day By conservativefiringline.com Published On :: Tue, 12 Nov 2024 23:15:42 +0000 The following article, INTERNal Stupidity: Pentagon Interns Post Pic of Chinese Plane on Veteran’s Day, was first published on Conservative Firing Line. A knock-off Chinese plane similar to the US F-35 was inadvertently posted by the F-35 JPO (Joint Program Office) on Veteran’s Day. Somebody needs to help this administration figure out some basics …oh wait, a new administration is coming in January. Good. The JPO Strike Force account had no clue what they did until an … Continue reading INTERNal Stupidity: Pentagon Interns Post Pic of Chinese Plane on Veteran’s Day ... Full Article Opinion Chinese plane F-35 Fighter jet Joint Program Office Joint Strike Force
tera VETERAN’S DAY: You’re Gonna Love What One Patriotic Coffee Company Did For The Marines! By clashdaily.com Published On :: Mon, 11 Nov 2024 10:30:41 +0000 Did you know that the Marines just celebrated their 249th birthday? This is one helluva tribute. The post VETERAN’S DAY: You’re Gonna Love What One Patriotic Coffee Company Did For The Marines! appeared first on Clash Daily. Full Article Videos
tera Reiterating our Use Restrictions Policy By signalvnoise.com Published On :: Mon, 18 Jan 2021 17:11:00 +0000 The attack on the US Capitol, and subsequent threats of violence surrounding the inauguration of the new US administration, has moved us to reflect and reacquaint ourselves with the reality that however good the maker’s intentions, technology can amplify the ability to cause great harm. This includes us and our products at Basecamp. Therefore, we… keep reading Full Article Uncategorized
tera Footage of Jill Biden and Kamala Harris Sitting Side-by-Side at Veterans Day Event Goes Viral By www.westernjournal.com Published On :: Mon, 11 Nov 2024 23:03:57 +0000 There appeared to be a definite frost between first lady Jill Biden and Vice President Kamala Harris during a Veterans Day event at Arlington National Cemetery on Monday. A close-up […] The post Footage of Jill Biden and Kamala Harris Sitting Side-by-Side at Veterans Day Event Goes Viral appeared first on The Western Journal. Full Article Commentary 2024 election Jill Biden Joe Biden Kamala Harris Veterans Day
tera Toward a Comprehensive Atlas of the Physical Interactome of Saccharomyces cerevisiae By www.mcponline.org Published On :: 2007-03-01 Sean R. CollinsMar 1, 2007; 6:439-450Research Full Article
tera Can Multilateralism Survive? By f1.media.brightcove.com Published On :: Tue, 06 Nov 2018 00:00:00 +0000 Full Article
tera Pinpointing How Genes Interact By www.ams.org Published On :: Thu, 21 Oct 2021 14:38:14 -0400 Lorin Crawford explains how he uses math to analyze interactions between genes. Your DNA (the biological instruction manual in all of your cells) contains a mind-boggling amount of information represented in roughly 20,000 genes that encode proteins, plus a similar number of genes with other functions. As the cost of analyzing an individual's DNA has plummeted, it has become possible to search the entire human genome for genetic variants that are associated with traits such as height or susceptibility to certain diseases. Sometimes, one gene has a straightforward impact on the trait. But in many cases, the effect of one gene variant depends on which variants of other genes are present, a phenomenon called "epistasis." Studying such interactions involves huge datasets encompassing the DNA of hundreds of thousands of people. Mathematically, that requires time-intensive calculations with massive matrices and a good working knowledge of statistics. Full Article
tera Representative cancer-associated U2AF2 mutations alter RNA interactions and splicing [Molecular Bases of Disease] By www.jbc.org Published On :: 2020-12-11T00:06:20-08:00 High-throughput sequencing of hematologic malignancies and other cancers has revealed recurrent mis-sense mutations of genes encoding pre-mRNA splicing factors. The essential splicing factor U2AF2 recognizes a polypyrimidine-tract splice-site signal and initiates spliceosome assembly. Here, we investigate representative, acquired U2AF2 mutations, namely N196K or G301D amino acid substitutions associated with leukemia or solid tumors, respectively. We determined crystal structures of the wild-type (WT) compared with N196K- or G301D-substituted U2AF2 proteins, each bound to a prototypical AdML polypyrimidine tract, at 1.5, 1.4, or 1.7 Å resolutions. The N196K residue appears to stabilize the open conformation of U2AF2 with an inter-RNA recognition motif hydrogen bond, in agreement with an increased apparent RNA-binding affinity of the N196K-substituted protein. The G301D residue remains in a similar position as the WT residue, where unfavorable proximity to the RNA phosphodiester could explain the decreased RNA-binding affinity of the G301D-substituted protein. We found that expression of the G301D-substituted U2AF2 protein reduces splicing of a minigene transcript carrying prototypical splice sites. We further show that expression of either N196K- or G301D-substituted U2AF2 can subtly alter splicing of representative endogenous transcripts, despite the presence of endogenous, WT U2AF2 such as would be present in cancer cells. Altogether, our results demonstrate that acquired U2AF2 mutations such as N196K and G301D are capable of dysregulating gene expression for neoplastic transformation. Full Article
tera Determinants of replication protein A subunit interactions revealed using a phosphomimetic peptide [Molecular Biophysics] By www.jbc.org Published On :: 2020-12-25T00:06:31-08:00 Replication protein A (RPA) is a eukaryotic ssDNA-binding protein and contains three subunits: RPA70, RPA32, and RPA14. Phosphorylation of the N-terminal region of the RPA32 subunit plays an essential role in DNA metabolism in processes such as replication and damage response. Phosphorylated RPA32 (pRPA32) binds to RPA70 and possibly regulates the transient RPA70-Bloom syndrome helicase (BLM) interaction to inhibit DNA resection. However, the structural details and determinants of the phosphorylated RPA32–RPA70 interaction are still unknown. In this study, we provide molecular details of the interaction between RPA70 and a mimic of phosphorylated RPA32 (pmRPA32) using fluorescence polarization and NMR analysis. We show that the N-terminal domain of RPA70 (RPA70N) specifically participates in pmRPA32 binding, whereas the unphosphorylated RPA32 does not bind to RPA70N. Our NMR data revealed that RPA70N binds pmRPA32 using a basic cleft region. We also show that at least 6 negatively charged residues of pmRPA32 are required for RPA70N binding. By introducing alanine mutations into hydrophobic positions of pmRPA32, we found potential points of contact between RPA70N and the N-terminal half of pmRPA32. We used this information to guide docking simulations that suggest the orientation of pmRPA32 in complex with RPA70N. Our study demonstrates detailed features of the domain-domain interaction between RPA70 and RPA32 upon phosphorylation. This result provides insight into how phosphorylation tunes transient bindings between RPA and its partners in DNA resection. Full Article
tera Ascertaining the biochemical function of an essential pectin methylesterase in the gut microbe Bacteroides thetaiotaomicron [Metabolism] By www.jbc.org Published On :: 2020-12-25T00:06:31-08:00 Pectins are a major dietary nutrient source for the human gut microbiota. The prominent gut microbe Bacteroides thetaiotaomicron was recently shown to encode the founding member (BT1017) of a new family of pectin methylesterases essential for the metabolism of the complex pectin rhamnogalacturonan-II (RG-II). However, biochemical and structural knowledge of this family is lacking. Here, we showed that BT1017 is critical for the metabolism of an RG-II–derived oligosaccharide ΔBT1017oligoB generated by a BT1017 deletion mutant (ΔBT1017) during growth on carbohydrate extract from apple juice. Structural analyses of ΔBT1017oligoB using a combination of enzymatic, mass spectrometric, and NMR approaches revealed that it is a bimethylated nonaoligosaccharide (GlcA-β1,4-(2-O-Me-Xyl-α1,3)-Fuc-α1,4-(GalA-β1,3)-Rha-α1,3-Api-β1,2-(Araf-α1,3)-(GalA-α1,4)-GalA) containing components of the RG-II backbone and its side chains. We showed that the catalytic module of BT1017 adopts an α/β-hydrolase fold, consisting of a central twisted 10-stranded β-sheet sandwiched by several α-helices. This constitutes a new fold for pectin methylesterases, which are predominantly right-handed β-helical proteins. Bioinformatic analyses revealed that the family is dominated by sequences from prominent genera of the human gut microbiota, including Bacteroides and Prevotella. Our re-sults not only highlight the critical role played by this family of enzymes in pectin metabolism but also provide new insights into the molecular basis of the adaptation of B. thetaiotaomicron to the human gut. Full Article
tera Africa-Japan relations and evolving multilateralism By www.chathamhouse.org Published On :: Thu, 17 Nov 2022 15:52:14 +0000 Africa-Japan relations and evolving multilateralism 23 November 2022 — 9:00AM TO 10:30AM Anonymous (not verified) 17 November 2022 Online This panel discussion reflects on the outcomes of TICAD 8 in 2022 and looks forward to TICAD 9 in 2025. The eighth edition of the Tokyo International Conference on African Development (TICAD), held in Tunisia from 27–28 August 2022, marked the second time that Japan’s now-triennial summit was hosted in an African country, after TICAD 6 was held in Kenya in 2016. The summit was attended by 48 representatives of African countries and at least 20 heads of state and government and included a pledge by the Japanese government to commit $30 billion in public and private finance to Africa over the next three years. In reaffirming the three pillars of TICAD 8 – revolving around the economy, societal resilience, and peace and stability – the newly adopted Tunis Declaration (28 August 2022) also outlined some of the key projects underpinning Japan’s pledge, including a $4 billion fund for a Green Growth Initiative with Africa (GGA). 2023 will mark 30 years since the inception of TICAD in 1993 and ten years since the African Union (AU)’s adoption of its flagship Agenda 2063, on which the Tunis Declaration placed distinct emphasis. This panel discussion reflects on the outcomes of TICAD 8 in 2022 and looks forward to TICAD 9 in 2025, exploring wider developments in summitry, Africa-Asia relations, and modes of multilateralism. Questions explored include: How has international summitry evolved over the past three decades since the inception of TICAD in 1993, which represented the first periodic high-level summit engagement with Africa by a ‘non-traditional’ partner? Looking ahead to TICAD 9 in 2025, what are the priorities for enforcing the stated tenets of TICAD – ‘African ownership, international partnership, inclusivity and openness’ – in cooperation efforts? What lessons can be drawn from TICAD’s co-partnership approach (with the African Union Commission and others) – particularly given increasing calls for AU membership of the G20 and Prime Minister Kishida’s pledge at TICAD 8 to support a permanent African UNSC seat during its non-permanent membership in 2023–24? Beyond membership, what are the priorities for furthering agency? How are Africa-Asia relations evolving and diverging? How are Japan and other Asian countries perceived by different African countries? This event is the third in the Chatham House – Japan House London webinar series (2022-2023). The series is held in partnership with Japan House London. You can watch previous webinars from the series here. Full Article
tera Novel Proteomic Profiling of Epididymal Extracellular Vesicles in the Domestic Cat Reveals Proteins Related to Sequential Sperm Maturation with Differences Observed between Normospermic and Teratospermic Individuals By www.mcponline.org Published On :: 2020-12-01 Tricia RowlisonDec 1, 2020; 19:2090-2103Research Full Article
tera Unraveling the MAX2 Protein Network in Arabidopsis thaliana: Identification of the Protein Phosphatase PAPP5 as a Novel MAX2 Interactor By www.mcponline.org Published On :: 2020-12-28 Sylwia StrukDec 28, 2020; 0:RA119.001766v1-mcp.RA119.001766Research Full Article
tera Systematic identification of P. falciparum sporozoite membrane protein interactions reveals an essential role for the p24 complex in host infection By www.mcponline.org Published On :: 2020-12-22 Julia KnöckelDec 22, 2020; 0:RA120.002432v1-mcp.RA120.002432Research Full Article
tera On the robustness of graph-based clustering to random network alterations By www.mcponline.org Published On :: 2020-11-04 R. Greg StaceyNov 4, 2020; 0:RA120.002275v1-mcp.RA120.002275Research Full Article
tera Thyroglobulin interactome profiling defines altered proteostasis topology associated with thyroid dyshormonogenesis By www.mcponline.org Published On :: 2020-11-18 Madison T WrightNov 18, 2020; 0:RA120.002168v1-mcp.RA120.002168Research Full Article
tera A potential role for the Gsdf-eEF1{alpha} complex in inhibiting germ cell proliferation: A protein-interaction analysis in medaka (Oryzias latipes) from a proteomics perspective By www.mcponline.org Published On :: 2020-12-08 Xinting ZhangDec 8, 2020; 0:RA120.002306v1-mcp.RA120.002306Research Full Article