pm The built environment and early childhood development: qualitative evidence from disadvantaged Australian communities. By ezproxy.scu.edu.au Published On :: Sat, 01 Apr 2023 00:00:00 -0400 Children's Geographies; 04/01/2023(AN 163915536); ISSN: 14733285Academic Search Premier Full Article POOR communities BUILT environment CHILD development COMMUNITIES GEOGRAPHIC information systems PUBLIC spaces
pm Policy development for children in Ethiopia: progress and next steps. By ezproxy.scu.edu.au Published On :: Sat, 01 Apr 2023 00:00:00 -0400 Children's Geographies; 04/01/2023(AN 163915528); ISSN: 14733285Academic Search Premier Full Article ETHIOPIA CHILD development YOUNG adults PUBLIC welfare policy GOVERNMENT policy WELL-being
pm An urban neighbourhood framework for realising progress towards the New Urban Agenda for equitable early childhood development. By ezproxy.scu.edu.au Published On :: Fri, 01 Dec 2023 00:00:00 -0500 Children's Geographies; 12/01/2023(AN 174964051); ISSN: 14733285Academic Search Premier Full Article CHILD development NEIGHBORHOODS CHILDREN with developmental disabilities PUBLIC transit OPEN spaces PUBLIC spaces
pm Multispecies collaboratories: reconfiguring children's more-than-human entanglement with colonization, urban development and climate change. By ezproxy.scu.edu.au Published On :: Thu, 01 Feb 2024 00:00:00 -0500 Children's Geographies; 02/01/2024(AN 175911766); ISSN: 14733285Academic Search Premier Full Article VICTORIA LONDON (Ont.) URBAN climatology GREEN movement INDIGENOUS peoples RECIPROCITY (Psychology) KINSHIP INDIGENOUS children
pm School-Based Victimization Prevention Education Programs for Children and Youth With Intellectual and Developmental Disabilities: A Scoping Review By ifp.nyu.edu Published On :: Sun, 03 Nov 2024 08:37:53 +0000 Child Maltreatment, Ahead of Print. Youth with intellectual and developmental disabilities (IDD) are at significantly higher risk of experiencing multiple types of interpersonal victimization across their lifespan compared to their peers without IDD. Despite the extensive literature on efficacy of prevention education programs for children without IDD, very little is known about comparable programs for […] The post School-Based Victimization Prevention Education Programs for Children and Youth With Intellectual and Developmental Disabilities: A Scoping Review was curated by information for practice. Full Article Meta-analyses - Systematic Reviews
pm Guidance | Learning and Development Support Scheme for the adult social care workforce: a guide for employers By ifp.nyu.edu Published On :: Wed, 23 Oct 2024 13:58:38 +0000 The post Guidance | Learning and Development Support Scheme for the adult social care workforce: a guide for employers was curated by information for practice. Full Article Guidelines Plus
pm World survey on the role of women in development By ifp.nyu.edu Published On :: Sat, 02 Nov 2024 03:56:30 +0000 The post World survey on the role of women in development was curated by information for practice. Full Article Grey Literature
pm Africa Small Grants programme: Palliative Care Development in Africa (Due by 31 Oct) By ifp.nyu.edu Published On :: Tue, 15 Oct 2024 03:52:38 +0000 The post Africa Small Grants programme: Palliative Care Development in Africa (Due by 31 Oct) was curated by information for practice. Full Article Funding
pm 2025-26 National Urban Fellows Program Now Accepting Applications: Fully Funded Master’s & Leadership Development By ifp.nyu.edu Published On :: Tue, 22 Oct 2024 14:04:46 +0000 The post 2025-26 National Urban Fellows Program Now Accepting Applications: Fully Funded Master’s & Leadership Development was curated by information for practice. Full Article Funding
pm Official Development Assistance: Global Innovation Fellowships (Expressions of interest must be submitted by 6 Nov) By ifp.nyu.edu Published On :: Tue, 29 Oct 2024 03:34:53 +0000 The post Official Development Assistance: Global Innovation Fellowships (Expressions of interest must be submitted by 6 Nov) was curated by information for practice. Full Article Funding
pm Part Time Undergraduate - Education and Research Enablement Development Engineer, Part-time Undergraduate, Cambridge, UK, Research By careers.peopleclick.com Published On :: Monday, November 23, 2020 5:29:17 PM EST Part Time Undergraduate (with first year as full-time placement) - Arm Education and Research Enablement Development EngineerArm has fuelled the smartphone revolution by creating clever compute power that fits in your pocket. Would you like to be part of the next revolution? Our programme is now open for applications! We want to hear from curious and enthusiastic candidates interested in working with us on the future generations of compute.About Arm and Arm EducationAs the industry's leading supplier of microprocessor technology, Arm provides efficient, low-power chip intelligence making affordable, easy-to-use electronic innovations come to life. Our engineers design and develop CPUs, graphics processors, complex system IP, supporting software development tools, and physical libraries.The Arm Education and Research Enablement offers high quality teaching and training materials to universities worldwide, and we are now seeking a Part Time Undergraduate to work with us in developing our offer. Your work will involve creating courseware to aid teaching based on Arm and partner technologies, as well as supporting technical customer inquiries. You will also develop software tools for automation, and work with internal and external partners to guarantee accurate legal and quality standards. Full Article
pm Disability Inclusion in Development Efforts: Analyzing the United States Agency for International Development’s Funding Solicitations for Evidence of Inclusive Practices By ifp.nyu.edu Published On :: Tue, 05 Nov 2024 02:28:36 +0000 Journal of Disability Policy Studies, Ahead of Print. In 1997, the United States Agency for International Development established a policy focused on including people with disabilities in its development efforts. For the past two decades, this initiative has been echoed globally, yet research on its effectiveness remains limited. This study revisits a previous 2015 analysis […] The post Disability Inclusion in Development Efforts: Analyzing the United States Agency for International Development’s Funding Solicitations for Evidence of Inclusive Practices was curated by information for practice. Full Article Journal Article Abstracts
pm Policast: Nurses say they need more protective equipment By www.mprnews.org Published On :: Wed, 20 May 2020 15:17:50 +0000 Nurses say they need more protective equipment; Asian owned businesses have been especially hard hit Full Article
pm DNR and residents sue to block controversial resort development outside Ely By www.mprnews.org Published On :: Sun, 27 Oct 2024 13:00:00 +0000 State environmental regulators and a group of northeastern Minnesota residents have filed separate lawsuits to block a proposed $45 million resort development near Ely and the Boundary Waters Canoe Area. Full Article
pm SFMO investigation leads to indictment against fire safety equipment installer By www.tdi.texas.gov Published On :: Mon, 3 Apr 2023 00:00:00 CDT Following an investigation by the State Fire Marshal’s Office (SFMO), a Jasper County grand jury indicted a fire safety equipment installer on two counts of tampering with a government document. Full Article
pm Dakota continues to accelerate growth and development By www.retailtechnologyreview.com Published On :: RetailTechnologyReview.com spoke with Dakota Integrated Solutions’ commercial director, Philip Jarrett, and recently appointed key account manager, Peter Jenkins, about the company’s current growth trajectory, including new partnerships and strategic technology sweet spots. Full Article Data Capture Retail Supply Chain Mobile Computers
pm Global smartphone market decline softens as shipments drop 10% in Q2 2023 By www.retailtechnologyreview.com Published On :: Canalys's latest research reveals that the worldwide smartphone market fell by 10% to 258.2 million units in Q2 2023, showing a slowdown in decline. Full Article Mobile Computers Critical Issues
pm Global tablet shipments up 8% sequentially as market revives before holiday season By www.retailtechnologyreview.com Published On :: According to the latest Canalys data, worldwide tablet shipments reached 33 million in Q3 2023, marking a 7% annual decline. But this represents an 8% sequential increase, reflecting a revival of the tablet market ahead of the crucial holiday season and the strong performance of new entrants in the space. Full Article Mobile Computers Critical Issues
pm Exploring The Distinctions, Applications, And Development of RFID And NFC Systems By www.retailtechnologyreview.com Published On :: By Alastair Bright, freelance writer.Radio Frequency Identification (RFID) and Near Field Communication (NFC) are two wireless communication technologies that enable data exchange through radio waves. While NFC is primarily used in contactless payments, RFID is widely utilized for asset and location tracking. Both technologies, however, also have versatile applications beyond their primary uses. Full Article RFID Data Capture Retail Supply Chain
pm RAIN Alliance reports 32% Increase in global RAIN RFID chip shipments as adoption and usage diversifies across multiple industries By www.retailtechnologyreview.com Published On :: RAIN Alliance, the non-profit industry organisation supporting the development and adoption of Ultra High Frequency (UHF) Radio Frequency Identification (RFID), has announced that 44.8bn RAIN RFID tag chips were shipped globally in 2023.This figure represents year-on-year growth of 32%, and reflects increasing demand across a variety of industries.... Full Article RFID Data Capture Critical Issues
pm RAIN RFID tag chip shipments forecast to reach 115bn units by 2028, a year-on-year growth of 20.4% By www.retailtechnologyreview.com Published On :: RAIN Alliance, the non-profit industry organisation supporting the development and adoption of Ultra High Frequency (UHF) Radio Frequency Identification (RFID), has published its bi-annual research report ‘Global Markets & Applications for RAIN RFID Solutions’, produced by VDC Research. Full Article RFID Data Capture Critical Issues
pm KIOSK Information Systems Europe boosts business development and customer support with new staff By www.retailtechnologyreview.com Published On :: The Posiflex Group has responded to rising demand from customers in Europe by recruiting a seasoned executive to boost the European sales and marketing operations in its KIOSK subsidiary, a manufacturer of kiosks and interactive displays. Full Article Kiosk Technology
pm BIXOLON and G Rigging & Lighting Ltd partner to improve event equipment management with barcode labelling solutions By www.retailtechnologyreview.com Published On :: BIXOLON Europe GmbH, a subsidiary of BIXOLON, a global leader in advanced receipt, label and mobile printers, has partnered with G Rigging & Lighting Ltd, an independent event specialist based in the UK. Full Article Print and Label
pm Former PMC Wagner commander joins Chechen fighters at Akhmat unit By english.pravda.ru Published On :: Thu, 11 Apr 2024 21:49:00 +0300 As many as 3,000 Wagner fighters joined the Akhmat special forces of the 2nd Army Corps of the Russian Armed Forces. Their commander Ratibor gathers people who will carry out the "tasks of the state.” The head of Chechnya, Ramzan Kadyrov, reported on Telegram that one of the commanders of the Wagner Group with call sign Ratibor would join the Akhmat special forces and bring in 3,000 fighters along with him too. The agreement was reached as a result of negotiations between the deputy commander of the 2nd Army Corps of the Russian Armed Forces, Apta Alaudinov, and Wagner commander, Alexander Kuznetsov (Ratibor). The Russian Defence Ministry approved the initiative. Kadyrov spoke of Ratibor as "an old comrade with whom he had fought side by side before.” Full Article Incidents
pm Latest Hobbit developments By www.talkorigins.org Published On :: There's a new paper out on the brain of Homo floresiensis, which concludes that it really is a new species; there are a number of new article about the debate over the fossils; and the fossils have finally been returned to their owners. Full Article
pm Couchbase Capella advancements fuel development of adaptive applications By www.retailtechnologyreview.com Published On :: Couchbase, Inc., the cloud database platform company, has launched Capella Columnar on AWS, which helps organisations streamline the development of adaptive applications by enabling real-time data analysis alongside operational workloads within a single database platform. Full Article Data Capture Retail Supply Chain
pm Discovery of 2,586 Orphan Genes in Rosa Chinensis Provides Insights Into Stress Adaptation and Flower Development By www.newswise.com Published On :: Wed, 13 Nov 2024 01:50:12 EST A research team has identified 2,586 orphan genes (OGs) in Rosa chinensis, offering new insights into the role of these unique genes in flower development, stress response, and environmental adaptation. Full Article
pm Discovery of 2,586 Orphan Genes in Rosa Chinensis Provides Insights Into Stress Adaptation and Flower Development By www.newswise.com Published On :: Wed, 13 Nov 2024 01:50:12 EST A research team has identified 2,586 orphan genes (OGs) in Rosa chinensis, offering new insights into the role of these unique genes in flower development, stress response, and environmental adaptation. Full Article
pm PM Han in Hangeul Day Speech: Nation Has Duty to Further Develop Korean Alphabet By world.kbs.co.kr Published On :: Wed, 09 Oct 2024 14:17:51 +0900 [Culture] : Prime Minister Han Duck-soo said the nation has a duty to further develop the Korean alphabet, hangeul, which has gained worldwide recognition as a unique writing system. At a government ceremony marking the 578th Hangeul Day on Wednesday, Han raised concerns about “indifference to the Korean language” ...[more...] Full Article Culture
pm How to Get Rid of Chipmunks By home.howstuffworks.com Published On :: Wed, 24 Jan 2024 18:06:11 -0500 Though they may be cute, if chipmunks get into your garden you'll have to get rid of them before they cause damage. Learn about how to get rid of chipmunks in this article. Full Article
pm Product development: properties and resources - British Geological Survey By news.google.com Published On :: Wed, 23 Sep 2020 13:28:59 GMT Product development: properties and resources British Geological Survey Full Article
pm International geoscience research and development - British Geological Survey By news.google.com Published On :: Sat, 20 Jan 2024 09:25:00 GMT International geoscience research and development British Geological Survey Full Article
pm Local MP checks in on development of new geothermal 'living laboratory' in Nottinghamshire - British Geological Survey By news.google.com Published On :: Thu, 19 Sep 2024 07:00:00 GMT Local MP checks in on development of new geothermal 'living laboratory' in Nottinghamshire British Geological Survey Full Article
pm A new modular framework for high-level application development at HEPS By journals.iucr.org Published On :: 2024-02-01 As a representative of the fourth-generation light sources, the High Energy Photon Source (HEPS) in Beijing, China, utilizes a multi-bend achromat lattice to obtain an approximately 100 times emittance reduction compared with third-generation light sources. New technologies bring new challenges to operate the storage ring. In order to meet the beam commissioning requirements of HEPS, a new framework for the development of high-level applications (HLAs) has been created. The key part of the new framework is a dual-layer physical module to facilitate the seamless fusion of physical simulation models with the real machine, allowing for fast switching between different simulation models to accommodate the various simulation scenarios. As a framework designed for development of physical applications, all variables are based on physical quantities. This allows physicists to analytically assess measurement parameters and optimize machine parameters in a more intuitive manner. To enhance both extensibility and adaptability, a modular design strategy is utilized, partitioning the entire framework into discrete modules in alignment with the requirements of HLA development. This strategy not only facilitates the independent development of each module but also minimizes inter-module coupling, thereby simplifying the maintenance and expansion of the entire framework. To simplify the development complexity, the design of the new framework is implemented using Python and is called Python-based Accelerator Physics Application Set (Pyapas). Taking advantage of Python's flexibility and robust library support, we are able to develop and iterate quickly, while also allowing for seamless integration with other scientific computing applications. HLAs for both the HEPS linac and booster have been successfully developed. During the beam commissioning process at the linac, Pyapas's ease of use and reliability have significantly reduced the time required for the beam commissioning operators. As a development framework for HLA designed for the new-generation light sources, Pyapas has the versatility to be employed with HEPS, as well as with other comparable light sources, due to its adaptability. Full Article text
pm Development of the multiplex imaging chamber at PAL-XFEL By journals.iucr.org Published On :: 2024-03-22 Various X-ray techniques are employed to investigate specimens in diverse fields. Generally, scattering and absorption/emission processes occur due to the interaction of X-rays with matter. The output signals from these processes contain structural information and the electronic structure of specimens, respectively. The combination of complementary X-ray techniques improves the understanding of complex systems holistically. In this context, we introduce a multiplex imaging instrument that can collect small-/wide-angle X-ray diffraction and X-ray emission spectra simultaneously to investigate morphological information with nanoscale resolution, crystal arrangement at the atomic scale and the electronic structure of specimens. Full Article text
pm Development of dual-beamline photoelectron momentum microscopy for valence orbital analysis By journals.iucr.org Published On :: 2024-04-15 The soft X-ray photoelectron momentum microscopy (PMM) experimental station at the UVSOR Synchrotron Facility has been recently upgraded by additionally guiding vacuum ultraviolet (VUV) light in a normal-incidence configuration. PMM offers a very powerful tool for comprehensive electronic structure analyses in real and momentum spaces. In this work, a VUV beam with variable polarization in the normal-incidence geometry was obtained at the same sample position as the soft X-ray beam from BL6U by branching the VUV beamline BL7U. The valence electronic structure of the Au(111) surface was measured using horizontal and vertical linearly polarized (s-polarized) light excitations from BL7U in addition to horizontal linearly polarized (p-polarized) light excitations from BL6U. Such highly symmetric photoemission geometry with normal incidence offers direct access to atomic orbital information via photon polarization-dependent transition-matrix-element analysis. Full Article text
pm Mapping of lithium ion concentrations in 3D structures through development of in situ correlative imaging of X-ray Compton scattering-computed tomography By journals.iucr.org Published On :: 2024-06-05 Understanding the correlation between chemical and microstructural properties is critical for unraveling the fundamental relationship between materials chemistry and physical structures that can benefit materials science and engineering. Here, we demonstrate novel in situ correlative imaging of the X-ray Compton scattering computed tomography (XCS-CT) technique for studying this fundamental relationship. XCS-CT can image light elements that do not usually exhibit strong signals using other X-ray characterization techniques. This paper describes the XCS-CT setup and data analysis method for calculating the valence electron momentum density and lithium-ion concentration, and provides two examples of spatially and temporally resolved chemical properties inside batteries in 3D. XCS-CT was applied to study two types of rechargeable lithium batteries in standard coin cell casings: (1) a lithium-ion battery containing a cathode of bespoke microstructure and liquid electrolyte, and (2) a solid-state battery containing a solid-polymer electrolyte. The XCS-CT technique is beneficial to a wide variety of materials and systems to map chemical composition changes in 3D structures. Full Article text
pm Development of a portable and cost-effective femtosecond fibre laser synchronizable with synchrotron X-ray pulses By journals.iucr.org Published On :: 2024-06-20 This study introduces a compact, portable femtosecond fibre laser system designed for synchronization with SPring-8 synchrotron X-ray pulses in a uniform filling mode. Unlike traditional titanium–sapphire mode-locked lasers, which are fixed installations, our system utilizes fibre laser technology to provide a practical alternative for time-resolved spectroscopy, striking a balance between usability, portability and cost-efficiency. Comprehensive evaluations, including pulse characterization, timing jitter and frequency stability tests revealed a centre wavelength of 1600 nm, a pulse energy of 4.5 nJ, a pulse duration of 35 fs with a timing jitter of less than 9 ps, confirming the suitability of the system for time-resolved spectroscopic studies. This development enhances the feasibility of experiments that combine synchrotron X-rays and laser pulses, offering significant scientific contributions by enabling more flexible and diverse research applications. Full Article text
pm Development and commissioning of a broadband online X-ray spectrometer for the SXFEL Facility By journals.iucr.org Published On :: 2024-07-29 A broadband online X-ray spectrometer has been designed and commissioned at the SUD beamline of the Shanghai Soft X-ray Free-Electron Laser Facility, which can deliver both SASE and seeded FEL pulses to user experiments, spanning the photon energy range of 50–620 eV. The resolving powers of the spectrometer calibrated via online measurement at 92 eV and 249 eV are ∼20000 and ∼15000, respectively, and the absolute photon energy is characterized by an electron time-of-flight spectrometer. The high energy resolution provided by the spectrometer can differentiate the fine structure in the FEL spectrum, to determine its pulse length. Full Article text
pm Development of a high-performance and cost-effective in-vacuum undulator By journals.iucr.org Published On :: 2024-08-01 In-vacuum undulators (IVUs), which have become an essential tool in synchrotron radiation facilities, have two technical challenges toward further advancement: one is a strong attractive force between top and bottom magnetic arrays, and the other is a stringent requirement on magnetic materials to avoid demagnetization. The former imposes a complicated design on mechanical and vacuum structures, while the latter limits the possibility of using high-performance permanent magnets. To solve these issues, a number of technical developments have been made, such as force cancellation and modularization of magnetic arrays, and enhancement of resistance against demagnetization by means of a special magnetic circuit. The performance of a new IVU built upon these technologies has revealed their effectiveness for constructing high-performance IVUs in a cost-effective manner. Full Article text
pm Development of an X-ray ionization beam position monitor for PAL-XFEL soft X-rays By journals.iucr.org Published On :: 2024-07-29 The Pohang Accelerator Laboratory X-ray Free-Electron Laser (PAL-XFEL) operates hard X-ray and soft X-ray beamlines for conducting scientific experiments providing intense ultrashort X-ray pulses based on the self-amplified spontaneous emission (SASE) process. The X-ray free-electron laser is characterized by strong pulse-to-pulse fluctuations resulting from the SASE process. Therefore, online photon diagnostics are very important for rigorous measurements. The concept of photo-absorption and emission using solid materials is seldom considered in soft X-ray beamline diagnostics. Instead, gas monitoring detectors, which utilize the photo-ionization of noble gas, are employed for monitoring the beam intensity. To track the beam position at the soft X-ray beamline in addition to those intensity monitors, an X-ray ionization beam position monitor (XIBPM) has been developed and characterized at the soft X-ray beamline of PAL-XFEL. The XIBPM utilizes ionization of either the residual gas in an ultra-high-vacuum environment or injected krypton gas, along with a microchannel plate with phosphor. The XIBPM was tested separately for monitoring horizontal and vertical beam positions, confirming the feasibility of tracking relative changes in beam position both on average and down to single-shot measurements. This paper presents the basic structure and test results of the newly developed non-invasive XIBPM. Full Article text
pm Development and performance simulations of a soft X-ray and XUV split-and-delay unit at beamlines FL23/24 at FLASH2 for time-resolved two-color pump–probe experiments By journals.iucr.org Published On :: 2024-08-05 The split-and-delay unit (SDU) at FLASH2 will be upgraded to enable the simultaneous operation of two temporally, spatially and spectrally separated probe beams when the free-electron laser undulators are operated in a two-color scheme. By means of suitable thin filters and an optical grating beam path a wide range of combinations of photon energies in the spectral range from 150 eV to 780 eV can be chosen. In this paper, simulations of the spectral transmission and performance parameters of the filter technique are discussed, along with a monochromator with dispersion compensation presently under construction. Full Article text
pm Development of a flat jet delivery system for soft X-ray spectroscopy at MAX IV By journals.iucr.org Published On :: 2024-08-22 One of the most challenging aspects of X-ray research is the delivery of liquid sample flows into the soft X-ray beam. Currently, cylindrical microjets are the most commonly used sample injection systems for soft X-ray liquid spectroscopy. However, they suffer from several drawbacks, such as complicated geometry due to their curved surface. In this study, we propose a novel 3D-printed nozzle design by introducing microscopic flat sheet jets that provide micrometre-thick liquid sheets with high stability, intending to make this technology more widely available to users. Our research is a collaboration between the EuXFEL and MAX IV research facilities. This collaboration aims to develop and refine a 3D-printed flat sheet nozzle design and a versatile jetting platform that is compatible with multiple endstations and measurement techniques. Our flat sheet jet platform improves the stability of the jet and increases its surface area, enabling more precise scanning and differential measurements in X-ray absorption, scattering, and imaging applications. Here, we demonstrate the performance of this new arrangement for a flat sheet jet setup with X-ray photoelectron spectroscopy, photoelectron angular distribution, and soft X-ray absorption spectroscopy experiments performed at the photoemission endstation of the FlexPES beamline at MAX IV Laboratory in Lund, Sweden. Full Article text
pm Development of an advanced in-line multilayer deposition system at Diamond Light Source By journals.iucr.org Published On :: 2024-08-09 A state-of-the-art multilayer deposition system with a 4200 mm-long linear substrate translator housed within an ultra-high vacuum chamber has been developed. This instrument is engineered to produce single and multilayer coatings, accommodating mirrors up to 2000 mm in length through the utilization of eight rectangular cathodes. To ensure the quality and reliability of the coatings, the system incorporates various diagnostic tools for in situ thickness uniformity and stress measurement. Furthermore, the system features an annealing process capable of heating up to 700°C within the load-lock chamber. The entire operation, including pump down, deposition and venting processes, is automated through user-friendly software. In addition, all essential log data, power of sputtering source, working pressure and motion positions are automatically stored for comprehensive data analysis. Preliminary commissioning results demonstrate excellent lateral film thickness uniformity, achieving 0.26% along the translation direction over 1500 mm in dynamic mode. The multilayer deposition system is poised for use in fabricating periodic, lateral-graded and depth-graded multilayers, specifically catering to the beamlines for diverse scientific applications at Diamond Light Source. Full Article text
pm Crystal clear: the impact of crystal structure in the development of high-performance organic semiconductors By journals.iucr.org Published On :: 2024-10-31 Full Article text
pm The High-Pressure Freezing Laboratory for Macromolecular Crystallography (HPMX), an ancillary tool for the macromolecular crystallography beamlines at the ESRF By journals.iucr.org Published On :: 2024-01-24 This article describes the High-Pressure Freezing Laboratory for Macromolecular Crystallography (HPMX) at the ESRF, and highlights new and complementary research opportunities that can be explored using this facility. The laboratory is dedicated to investigating interactions between macromolecules and gases in crystallo, and finds applications in many fields of research, including fundamental biology, biochemistry, and environmental and medical science. At present, the HPMX laboratory offers the use of different high-pressure cells adapted for helium, argon, krypton, xenon, nitrogen, oxygen, carbon dioxide and methane. Important scientific applications of high pressure to macromolecules at the HPMX include noble-gas derivatization of crystals to detect and map the internal architecture of proteins (pockets, tunnels and channels) that allows the storage and diffusion of ligands or substrates/products, the investigation of the catalytic mechanisms of gas-employing enzymes (using oxygen, carbon dioxide or methane as substrates) to possibly decipher intermediates, and studies of the conformational fluctuations or structure modifications that are necessary for proteins to function. Additionally, cryo-cooling protein crystals under high pressure (helium or argon at 2000 bar) enables the addition of cryo-protectant to be avoided and noble gases can be employed to produce derivatives for structure resolution. The high-pressure systems are designed to process crystals along a well defined pathway in the phase diagram (pressure–temperature) of the gas to cryo-cool the samples according to the three-step `soak-and-freeze method'. Firstly, crystals are soaked in a pressurized pure gas atmosphere (at 294 K) to introduce the gas and facilitate its interactions within the macromolecules. Samples are then flash-cooled (at 100 K) while still under pressure to cryo-trap macromolecule–gas complexation states or pressure-induced protein modifications. Finally, the samples are recovered after depressurization at cryo-temperatures. The final section of this publication presents a selection of different typical high-pressure experiments carried out at the HPMX, showing that this technique has already answered a wide range of scientific questions. It is shown that the use of different gases and pressure conditions can be used to probe various effects, such as mapping the functional internal architectures of enzymes (tunnels in the haloalkane dehalogenase DhaA) and allosteric sites on membrane-protein surfaces, the interaction of non-inert gases with proteins (oxygen in the hydrogenase ReMBH) and pressure-induced structural changes of proteins (tetramer dissociation in urate oxidase). The technique is versatile and the provision of pressure cells and their application at the HPMX is gradually being extended to address new scientific questions. Full Article text
pm Time-series analysis of rhenium(I) organometallic covalent binding to a model protein for drug development By journals.iucr.org Published On :: 2024-04-19 Metal-based complexes with their unique chemical properties, including multiple oxidation states, radio-nuclear capabilities and various coordination geometries yield value as potential pharmaceuticals. Understanding the interactions between metals and biological systems will prove key for site-specific coordination of new metal-based lead compounds. This study merges the concepts of target coordination with fragment-based drug methodologies, supported by varying the anomalous scattering of rhenium along with infrared spectroscopy, and has identified rhenium metal sites bound covalently with two amino acid types within the model protein. A time-based series of lysozyme-rhenium-imidazole (HEWL-Re-Imi) crystals was analysed systematically over a span of 38 weeks. The main rhenium covalent coordination is observed at His15, Asp101 and Asp119. Weak (i.e. noncovalent) interactions are observed at other aspartic, asparagine, proline, tyrosine and tryptophan side chains. Detailed bond distance comparisons, including precision estimates, are reported, utilizing the diffraction precision index supplemented with small-molecule data from the Cambridge Structural Database. Key findings include changes in the protein structure induced at the rhenium metal binding site, not observed in similar metal-free structures. The binding sites are typically found along the solvent-channel-accessible protein surface. The three primary covalent metal binding sites are consistent throughout the time series, whereas binding to neighbouring amino acid residues changes through the time series. Co-crystallization was used, consistently yielding crystals four days after setup. After crystal formation, soaking of the compound into the crystal over 38 weeks is continued and explains these structural adjustments. It is the covalent bond stability at the three sites, their proximity to the solvent channel and the movement of residues to accommodate the metal that are important, and may prove useful for future radiopharmaceutical development including target modification. Full Article text
pm Biophysical and structural study of La Crosse virus endonuclease inhibition for the development of new antiviral options By journals.iucr.org Published On :: 2024-04-24 The large Bunyavirales order includes several families of viruses with a segmented ambisense (−) RNA genome and a cytoplasmic life cycle that starts by synthesizing viral mRNA. The initiation of transcription, which is common to all members, relies on an endonuclease activity that is responsible for cap-snatching. In La Crosse virus, an orthobunyavirus, it has previously been shown that the cap-snatching endonuclease resides in the N-terminal domain of the L protein. Orthobunyaviruses are transmitted by arthropods and cause diseases in cattle. However, California encephalitis virus, La Crosse virus and Jamestown Canyon virus are North American species that can cause encephalitis in humans. No vaccines or antiviral drugs are available. In this study, three known Influenza virus endonuclease inhibitors (DPBA, L-742,001 and baloxavir) were repurposed on the La Crosse virus endonuclease. Their inhibition was evaluated by fluorescence resonance energy transfer and their mode of binding was then assessed by differential scanning fluorimetry and microscale thermophoresis. Finally, two crystallographic structures were obtained in complex with L-742,001 and baloxavir, providing access to the structural determinants of inhibition and offering key information for the further development of Bunyavirales endonuclease inhibitors. Full Article text
pm Current developments and trends in quantum crystallography By journals.iucr.org Published On :: 2024-06-18 Quantum crystallography is an emerging research field of science that has its origin in the early days of quantum physics and modern crystallography when it was almost immediately envisaged that X-ray radiation could be somehow exploited to determine the electron distribution of atoms and molecules. Today it can be seen as a composite research area at the intersection of crystallography, quantum chemistry, solid-state physics, applied mathematics and computer science, with the goal of investigating quantum problems, phenomena and features of the crystalline state. In this article, the state-of-the-art of quantum crystallography will be described by presenting developments and applications of novel techniques that have been introduced in the last 15 years. The focus will be on advances in the framework of multipole model strategies, wavefunction-/density matrix-based approaches and quantum chemical topological techniques. Finally, possible future improvements and expansions in the field will be discussed, also considering new emerging experimental and computational technologies. Full Article text
pm Development of MHz X-ray phase contrast imaging at the European XFEL By journals.iucr.org Published On :: The development of instrumentation as well as applications for megahertz X-ray phase contrast imaging at the Single Particles, Clusters, and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL are introduced here. Full Article text