African swine fever (ASF) is a highly contagious hemorrhagic viral disease of domestic and wild pigs that is responsible for serious economic and production losses. It is caused by the African swine fever virus (ASFV), a large and complex icosahedral DNA virus of the Asfarviridae family. Currently, there is no effective treatment or approved vaccine against the ASFV. pS273R, a specific SUMO-1 cysteine protease, catalyzes the maturation of the pp220 and pp62 polyprotein precursors into core-shell proteins. Here, we present the crystal structure of the ASFV pS273R protease at a resolution of 2.3 Å. The overall structure of the pS273R protease is represented by two domains named the "core domain" and the N-terminal "arm domain." The "arm domain" contains the residues from M1 to N83, and the "core domain" contains the residues from N84 to A273. A structure analysis reveals that the "core domain" shares a high degree of structural similarity with chlamydial deubiquitinating enzyme, sentrin-specific protease, and adenovirus protease, while the "arm domain" is unique to ASFV. Further, experiments indicated that the "arm domain" plays an important role in maintaining the enzyme activity of ASFV pS273R. Moreover, based on the structural information of pS273R, we designed and synthesized several peptidomimetic aldehyde compounds at a submolar 50% inhibitory concentration, which paves the way for the design of inhibitors to target this severe pathogen.

IMPORTANCE African swine fever virus, a large and complex icosahedral DNA virus, causes a deadly infection in domestic pigs. In addition to Africa and Europe, countries in Asia, including China, Vietnam, and Mongolia, were negatively affected by the hazards posed by ASFV outbreaks in 2018 and 2019, at which time more than 30 million pigs were culled. Until now, there has been no vaccine for protection against ASFV infection or effective treatments to cure ASF. Here, we solved the high-resolution crystal structure of the ASFV pS273R protease. The pS273R protease has a two-domain structure that distinguishes it from other members of the SUMO protease family, while the unique "arm domain" has been proven to be essential for its hydrolytic activity. Moreover, the peptidomimetic aldehyde compounds designed to target the substrate binding pocket exert prominent inhibitory effects and can thus be used in a potential lead for anti-ASFV drug development.

Grasses are among the most resilient plants, and some can survive prolonged desiccation in semiarid regions with seasonal rainfall. However, the genetic elements that distinguish grasses that are sensitive versus tolerant to extreme drying are largely unknown. Here, we leveraged comparative genomic approaches with the desiccation-tolerant grass Eragrostis nindensis and...

This is a video of musician and Instagrammer nathanleighsays performing Saria's Song (the Lost Woods theme) from The Legend Of Zelda: Ocarina Of Time on 24 partially empty wine bottles. How about that! Of course this would never work at my aunt's house on account of the bottles all playing the same note because they're empty. And the cats! Soooooo many cats. Keep going for the performance as well as a clip of Link learning Saria's song from the actual game.

From creamy gnudi to champagne macarons, the dishes at USA TODAY's Wine & Food Experience in Chicago didn't disappoint.

We love these products, and we hope you do too. E! has affiliate relationships, so we may get a small share of the revenue from your purchases. Items are sold by the retailer, not E!. One...

From quarantine vino survival guides to sharing a glass with celebs, these are the best wine podcasts to know

Where can we sign up?

London's wine shops and bars have shifted their booze online to beat the crippling financial effects of coronavirus. Abbie Moulton on the new way to drink responsibly...

We love these products, and we hope you do too. E! has affiliate relationships, so we may get a small share of the revenue from your purchases. Items are sold by the retailer, not E!. One...

The Federal Government is falling short on its own commitment to boost funding for services to keep Australia free from pests and disease.

We love these products, and we hope you do too. E! has affiliate relationships, so we may get a small share of the revenue from your purchases. Items are sold by the retailer, not E!. One...

#TTB just made changes to modernize the way that #wine, #distilledspirits, and #maltbeverages are labeled and advertised. KKB associate Dan Logan and partner Dan Dwyer highlight some of the key changes (and proposals that were rejected).

The post TTB Finalizes Portions of Modernization of Advertising and Labeling Regulations for Wine, Distilled Spirits, and Malt Beverages Rule appeared first on Kleinfeld Kaplan & Becker LLP.

Spanish company Lucirmás debuts Pure-Bottle, a fully recycled and recyclable table set which consists of a glass, lantern and spoon.

This eco-wine is bursting with red fruit aromas and vanilla. And it's minty finish is subtle yet clean so you won't mind a second glass. Which isn't a bad thing as this Pinot comes in under the $15 mark. And the winery is 100-percent solar-powered.

UNTI 2007 Syrah Benchland is dark, somewhat brooding like a radio-friendly Cure song. Within your glass you'll find flavors of grilled plum, anise and cocoa. The wine is unfined which means it is vegan.

A delicate balance of dark fruit, cocoa, pepper and mushroom from a sustainable vineyard that donates to AIDS and cancer patients.

Here's the manifesto by the Spanish wine industry to fight climate change by making wineries more eco-friendly. Vines are very sensitive to climate change and so their environment, landscape, culture and tradition need protecting.

The nose is dancing with floral highlights and warm peach aromas that you half expect it to be a dessert wine. But it's surprisingly elegant for a wine just under $10. The winery is 100-percent solar-powered, as is its sister winery, Jacuzzi.

Unti Vineyard's 2008 Grenache is smoky and spicy with cranberry and anise on the nose. Marionberry and a minty finish play well with the wine's acidity and refined tannins. And it's vegan!

This wine is brimming with bright fruit, dark undertones and those farmyard aromas common to most Pinots. With notes of pepper, strawberry and dried mint on the palette, this wine is as food-friendly as it is drinkable.

This eco-wine is thick with berries and molasses on the nose but the follow through is not your usual California cab. And for every acre of planted vineyard, Frei Brothers sets aside one acre to be preserved as natural wildlife habitat.

Wrath's 2010 Ex Anima Chardonnay is billowing with so much tropical fruit that you half expect Kokomo to start playing on the jukebox the second you uncork it. I swear this wine was a Piña Colada in its past life.

Hahn SLH Estate's 2010 Chef's Table Viognier is one of the most interesting whites you'll ever smell. It's completely intoxicating with notes of white peach, banana, honeydew and jasmine. Only 100 cases were made, so get this one quickly!

Frei's 2009 Chardonnay touches your nose with hints of rose water, jasmine and other floral delights. But on the palette, it is swimming with honey and sweet butter and just enough acidity to make it all work.

Wrath's 2010 Ex Vite Pinot Noir is complete, with lots of black cherry and red raspberry. A bit fuller than your typical Pinot, this wine is as drinkable as it is food friendly.

Scott Gundersen makes art with corks, using up to 9,000 of the old bottle-stoppers to create each beautiful portrait.

The Spanish porrón is a glass pitcher in the shape of a watering can that gets passed around at big events. That way, there is no need for plastic glasses or washing up!

The global production of wine isn't keeping up with demand.

The French agriculture ministry has sentenced Emmanuel Giboulot six months in jail for not taking preventative measures against a bacterial vine disease.

Fruit flies play a role in all those fruity flavors we detect as we take whiff of wine fumes. Find out how.

In another case for consumer right-to-know, a new study demonstrates big differences between wines depending on the production method

Just what we've been waiting for - an automated home fermentation device that can turn fruit or honey into wine, mead, or cider.

Perhaps our perceptions are predicated on the packaging.

Like our houses and our cars and our donuts, everything is bigger these days.

For the first time ever, insects took the place of cheese at an upscale gastronomical event.

A new organic winery for Pizzolato, designed by MADE, is built almost entirely out of local, sustainably harvested wood.

Lack of diversity makes grapes vulnerable to climate change.

"Naked wine," "raw wine," "biodynamic wine" have reached a feverish peak among trend-setting oenophiles. But what do all these labels mean, and are they actually better for the environment?

In case you're thinking of numbing your way through our dystopian future with a good glass of pinot...

Toronto project pushes every TreeHugger button

The natural chemicals in common foods and beverages can be turned into a useful antibacterial coating

The way we're doing things clearly doesn't work.

Stag’s Leap Wine Cellars celebrates the 40th anniversary of The Judgment of Paris, which helped to elevate the esteem of American wine worldwide

Stag’s Leap Wine Cellars celebrates the 40th anniversary of The Judgment of Paris, which helped to elevate the esteem of American wine worldwide

Bandra got its first outlet of The Wine Table in May last year. What does it offer? MRP Wine bar by glass & bottle and Wine education/training/food pairing with wines, etc. From imported - Ren, Beaujolais villages, Peppoli, Cono Sur, Torres, Salbach, Masi masianco, Robert Mondavi to domestic - Amaris reserve, Fratelli MS red, Fratelli Sangiovese, Moksh sauvignon blanc, M reserve, Raaya rose, the outlets offer all things wine.

Tignanello Italian, priced at Rs 20,000, is the most expensive buy. We walk in at 6 pm to douse our red craving, and a bite but the kitchen opens only at 7 pm. We order a Sileni pinot noir (Rs 630) and a Mokksh ren (Rs 396), a flagship brand of the store. The Sileni from New Zealand is light and perfumed and perfect for the rather early indulgence. The ren merlot is medium bodied, woody and deeper on the palate.

While our server gives us back stories of wines and vineyards, we’ll surely go back to try some whites next.

AT: Darvesh Royale Building, Perry Road, Pali Hill, Bandra West
CALL: +91 9167007667
TIMINGS: 10 am to 12.30 am
NOTE: The Wine Table didn't know we were there. The Guide reviews anonymously and pays for its meals

Catch up on all the latest Crime, National, International and Hatke news here. Also download the new mid-day Android and iOS apps to get latest updates

Social media was flooded with hilarious memes and witty jokes as India entered Lockdown 3.0 phase on Monday. With wine shops opening in red, orange and green zones, thousands of poeple thronged to the shops to stock up on alcohol.

Although wine shops have been advised to follow several guidelines and practice social distancing, hundreds of people lined up outside wine shops across the country as early as 5 and 6 in the morning. Netizens took to social networking sites to share their emotions as visuals of long ques emerged outside wine shops.

Neighbor uncle after spotting me in a queue at a liquor shop -#LiquorShops pic.twitter.com/9cuNN8g9dE

— नà¥Â€रà¤ÂÂÂÂÂœ . (@AjeebPrani) May 4, 2020

Here's what happens when neighbour uncle spots you at a liquor shop.

When liquor shop gets green signal #liquorshops #beer #LiquorShop pic.twitter.com/dF3OZg6O7j

— UTKARSH KHARE (@IAMUTKARSHKHARE) May 1, 2020

Happiness needs no bound when one hears about wine shops opening.

#wineshops
Alcohol may be man's worst enemy, but the bible says love your enemy. pic.twitter.com/2PN4Fvezpg

— Tulsi Nivas Bisen (@BisenTulsi) May 4, 2020

When wine shops promise to revive Indian economy amid coronavirus crisis.

Posted without comment. #liquor #liquorshops #Lockdown3 pic.twitter.com/NLHya73lYn

— Carona Mohapatra (@carona_m) May 4, 2020

This TikTok video will definitely tickle your funny bone.

Hilarious.... check out how he is craving for wine n chicken😂😂#wineshops pic.twitter.com/NUqyy8FDrg

— Bharatt Dilip Shitole 🇮🇳 (@bharatshitole) May 4, 2020

When alcohol and chicken are life amid lockdown.

Someone spent his month's salary today #wineshops pic.twitter.com/0tOCIUhoOL

— Abhishek Baadkar (@Ibaadu) May 4, 2020

So, this really happened?

#LiquorShops #wineshops #wine

*After getting 1 km long queues outside their shops*

Liquor shop owners to grocery shop owners : pic.twitter.com/bcXp8Svjhy

— JRism (@jiteshrochlani) May 4, 2020

Liquor shops owners upon seeing huge lines outside their shops.

The air in Bengaluru is so clear that I can even see the end of this liquor shop queue. #LiquorShops

— Ramesh Srivats (@rameshsrivats) May 4, 2020

When Twitter user could see long ques outside wine shops due to better air quality.

Me and boys after hijacking the wine shop 😂 #LiquorShops pic.twitter.com/BfNL2IiiHc

— Prakash Tiwari (@prakash__t) May 4, 2020

Here's what happens when one hijacks wine shops amid lockdown.

RED ZONE BE LIKE

APNA TIME AAYEGA#LiquorShops pic.twitter.com/5hgFbwsrX1

— 𝕳ð–ÂÂÂÂŽ𝖘𝖊𝖓𝖇𝖊𝖗ð–ÂÂÂÂÂŒ (@ImpossiBall) May 4, 2020

People staying in red and containment zones be like.

Corona?? What Corona?? Its Marriage type celebration with Band Bajaa as #LiquorShops open ðŸÂÂÂÂÂŽ·ðŸÂÂÂÂÂŽºðŸ˜‚🍻 pic.twitter.com/45hoFLZPqB

— Rosy (@rose_k01) May 4, 2020

When news of wine shops opening is celebrated with Band, Baaja and Baarat!

#LiquorShops owners waiting for their customers be like:
(Aa aa aaa aa aaa sound plays in background) pic.twitter.com/rDzpsa1D8W

— The Steno Boi (@Thestenoboi) May 4, 2020

Liquor owners waiting for customers be like.

Drinkers after getting the news that liquor shops are open in all zones#LiquorShops pic.twitter.com/gmfB4UyYqo

— Sociopath Memer (@sociopath_ladka) May 4, 2020

When Twitterati could not argue but agree with Mohnish Behl from Hum Saath Saath Hain.

The posts in this articles are personal views of the Twitter users. They do not reflect the opinions or views of mid-day online

Catch up on all the latest Crime, National, International and Hatke news here. Also download the new mid-day Android and iOS apps to get latest updates.

Mid-Day is now on Telegram. Click here to join our channel (@middayinfomedialtd) and stay updated with the latest news

Swine flu, the virus that caused panic in India in 2009, is back this season. In fact, in 2010, the WHO had warned of s

Coronavirus infections in northeast India are comparatively under control so far, the eight northeastern states are now affected by African Swine Flu (ASF).

Exports of Primary Prds - Meat of Swine in Brazil increased to 154 USD Million in April from 143.29 USD Million in February of 2020. Exports of Primary Prds - Meat of Swine in Brazil averaged 59.69 USD Million from 1989 until 2020, reaching an all time high of 183.05 USD Million in October of 2014 and a record low of 0.78 USD Million in November of 1990. This page includes a chart with historical data for Brazil Exports of : Primary Prds - Meat of Swine.