It won’t search for life, but rather determine whether conditions there could support it.

The post NASA’s Europa Clipper spacecraft will scour Jupiter moon for the ingredients for life appeared first on Boston.com.

After openers by Boston's DJ P1no and local singer Raveena, streaming sensation Tinashe very successfully brought 'Nasty' back.

The post Review & setlist: Boston matches Tinashe’s freak at MGM Music Hall appeared first on Boston.com.

I stood along the rail of an upper tier at the Nashville Convention Center, home of the 2009 AWCI Convention and Intex Expo trade show. I watched two middle-aged men approaching each other on opposing escalators, one going up, the other going down.

Panasonic in partnership with the Seattle Mariners announced the installation of a solar panel system comprised of 168 Panasonic HIT® Double solar panels at Safeco Field, the Seattle Mariners' home ballpark.

National Safe Skies Alliance performs its evaluations under an ISO 9001:2015-approved Quality Management System.

As a manufacturer headquartered in an island nation, Panasonic is keenly aware of the burden that is placed on the environment and the responsibility that every individual and corporation must assume in stewarding its health and preservation.

The new, larger location in the heart of the greater metro Nashville area replaces 21st Century’s existing warehouse in Smyrna, Tenn. 

Watertown, SD — A new video from the National Association of Tower Erectors stresses hazard awareness for technicians who work with small cellular antenna towers on new or existing structures.

Second-generation stevia extracts were all about high-purity Reb A. In contrast, today’s third-generation stevia extracts are based on better tasting steviol glycosides with the highest commercial value, Reb A, B, C, D and M. 

Solely says it used its signature, patented cold-pressing process to transform dried green bananas into a traditional fusilli shape and texture. The dark-colored Organic Green Banana Fusilli Pasta cooks to an al-dente texture in just four minutes and is ready to be topped with a sauce or other ingredients.

They might be small, but they have HUGE purchasing potential. Generation Alpha—that’s anyone born between 2010 and 2025—is shaping up to be one of most savvy and influential consumer demographics in history 

Yiannas’ appointment comes after the company announced it would permanently discontinue its liverwurst product and indefinitely close its Jarratt, Va., plant due to a Listeria monocytogenes outbreak.

Children's Geographies; 12/01/2023
(AN 174964058); ISSN: 14733285
Academic Search Premier

The Dutch Euthanasia law permits euthanasia in patients with advanced dementia lacking decisional capacity based on advance euthanasia directives. Nevertheless, physicians encounter difficulties assessing the … Read the full article ›

The post A qualitative focus group study on legal experts’ views regarding euthanasia requests based on an advance euthanasia directive was curated by information for practice.

by Wm. Steven Humphrey

Who's ready to have some fun? Well, the Mercury is here to help with FREE TICKETS to see some of Portland's best concerts and events—our way of saying thanks to our great readers and spread the word about some fantastic upcoming performances! (Psst... if you want to say thanks to the Mercury, please consider making a small monthly contribution to keep us alive and kickin'!) And oh boy, do we have some fun events coming at ya this week! CHECK IT OUT!

• Enter to WIN FREE TICKETS to see Reverend Horton Heat on February 27 at Crystal Ballroom!

Seeing REVEREND HORTON HEAT live is a transformative experience. Flames come off the guitars. Heat singes your skin. There’s nothing like the primal tribal rock & roll transfiguration of a Reverend Horton Heat show. Get your tickets now, or enter to win here!

Crystal Ballroom, 1332 W Burnside, Thurs Feb 27, 8 pm, $35-$50, all ages

• Enter to WIN FREE TICKETS to see Des Demonas on November 17 at Polaris Hall!

A favorite of Henry Rollins and Iggy Pop, Washington, DC’s DES DEMONAS blend post punk, funk, blues, psych rock, Afro beat, and more into their own unique sound — listen LIVE when they share new tunes from their highly-anticipated sophomore LP, APOCALYPTIC BOOM! BOOM! Get your tickets now or enter to win a free pair!

Polaris Hall, 635 N Killingsworth Ct, Sun Nov 17, 8 pm, $15, 21+

• Enter to WIN FREE TICKETS to see Karina Rykman on November 15 at Mississippi Studios!

She’s opened for Khruangbin, performed on America’s Got Talent, soundchecked with Phish at MSG, and much more — catch dynamic jam rock bassist Karina Rykman LIVE when she returns to Portland! Get your tickets now or enter to win a free pair!

Mississippi Studios, 3939 N Mississippi, Fri Nov 15, 9 pm, $20, 21+

• Enter to WIN FREE TICKETS to see Mason Jennings on November 16 at Mississippi Studios!

Hawai’i-born, Minneapolis-based folksinger Mason Jennings returns to Mississippi Studios with tunes from his latest record, Holy Dive, and beyond! Get your tickets now or enter to win a free pair!

Mississippi Studios, 3939 N Mississippi, Sat Nov 16, 8 pm, $30, 21+

• Enter to WIN FREE TICKETS to see The Bygones on November 17 at Aladdin Theater!

Luminous indie folk duo The Bygones share nostalgic songwriting from their debut, self-titled release! Get your tickets now or enter to win a free pair!

Aladdin Theater, 3017 SE Milwaukie, Sun Nov 17, 8 pm, $25, all ages

• Enter to WIN FREE TICKETS to see Sam Grisman Project on November 16 at Aladdin Theater!

A longtime Dawg Trio member with his father David Grisman, Bay Area bass player Sam Grisman upholds the legacy of Dawg music with his new musical project. Don’t miss their return to Portland! Get your tickets now or enter to win a free pair!

Aladdin Theater, 3017 SE Milwaukie, Sat Nov 16, 8 pm, $32.50, all ages

GOOD LUCK! Winners will be notified on Monday. Check back next week for more FREE TIX from the Mercury!

A news release says Heaven's Door Spirits will transform a 160-year-old church into the Heaven's Door Distillery and Center for the Arts, featuring the distillery, a whiskey library, a restaurant and a 360-seat live performance venue.

Folks, the yeehaw agenda has reached its absolute apex. Chris Rock, Rico Nasty, Vince Staples, Diplo and, of course, Billy Ray Cyrus guest in the video for the smash hit.

An Atlanta rapper's take on country music has inspired a mostly nonverbal Minnesota boy with autism to sing.

All this week, NHPR is talking with artists about their work and how the past year has changed the way they create art. For the latest installment of “ The Show Goes On ," All Things Considered host Peter Biello explores the colorful streetscape of Nashua.

El Departamento de Seguros de Texas (TDI, por su nombre y siglas en inglés) anunció a Dan Paschal como el Comisionado Adjunto de la recién creada división de Relaciones Externas. Esta división incluirá Comunicaciones (actualmente Asuntos Públicos) y Relaciones Gubernamentales (actualmente Asuntos de la Agencia).

REX Photo A deplorable statement has been made by hegumen of the Orthodox Docheiariou monastery on the Holy Mount Athos. The highest cathedral of the state may face destruction in case of an earthquake, which happen there quite often. The case is about Archistrategos Cathedral. As Archimandrite Gregorius (Zumis) revealed, the cathedral has fallen into the state of desolation after Greece left Athos to the mercy of fate. The authorities have not undertaken any measures to restore monuments so far. Centre for Maintenance of the Athos Heritage which was been established by the state, has been left without funding. According to the hegumen, monks of the monastery have been struggling for adoption of a plan on restoration of the Cathedral for as long as 28 years. But it has not been adopted still. And it is impossible to restore a huge Cathedral by themselves, for charity only.

Laysan Utyasheva, a famous gymnast, shared the recipe for a miraculous drink that she swears by. She only learned the recipe for this drink at the beginning of 2022, and since then she has been drinking it every morning to wake up quickly. Utyasheva claims that this drink helps her wake up better than five cups of coffee. In an interview on the YouTube show "Daring Cooking," Laysan Utyasheva explained that one morning, when she was going on a desert excursion, she really needed to wake up quickly, but five cups of coffee didn't do the trick. That's when she tried the new drink, which woke her up much better. The recipe for this drink is simple: add a pinch of turmeric, cinnamon, and lemon to warm water. This drink helps reduce swelling and boost immunity. However, Laysan Utyasheva warned that the taste of the drink is not very pleasant, but it is very effective.

Molybdenum- or tungsten-dependent formate dehydrogenases have emerged as significant catalysts for the chemical reduction of CO2 to formate, with biotechnological applications envisaged in climate-change mitigation. The role of Met405 in the active site of Desulfovibrio vulgaris formate dehydrogenase AB (DvFdhAB) has remained elusive. However, its proximity to the metal site and the conformational change that it undergoes between the resting and active forms suggests a functional role. In this work, the M405S variant was engineered, which allowed the active-site geometry in the absence of methionine Sδ interactions with the metal site to be revealed and the role of Met405 in catalysis to be probed. This variant displayed reduced activity in both formate oxidation and CO2 reduction, together with an increased sensitivity to oxygen inactivation.

Pseudoalteromonas fuliginea sp. PS47 is a recently identified marine bacterium that has extensive enzymatic machinery to metabolize polysaccharides, including a locus that targets pectin-like substrates. This locus contains a gene (locus tag EU509_03255) that encodes a pectin-degrading lyase, called PfPL1, that belongs to polysaccharide lyase family 1 (PL1). The 2.2 Å resolution X-ray crystal structure of PfPL1 reveals the compact parallel β-helix fold of the PL1 family. The back side of the core parallel β-helix opposite to the active site is a meandering set of five α-helices joined by lengthy loops. A comparison of the active site with those of other PL1 enzymes suggests a catalytic mechanism that is independent of metal ions, such as Ca2+, but that substrate recognition may require metal ions. Overall, this work provides the first structural insight into a pectinase of marine origin and the first structure of a PL1 enzyme in subfamily 2.

Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme in the pentose phosphate pathway. It has been extensively studied by biochemical and structural techniques. 13 X-ray crystal structures and five electron cryo-microscopy structures in the PDB are focused on in this topical review. Two F420-dependent glucose-6-phosphate dehydrogenase (FGD) structures are also reported. The significant differences between human and parasite G6PDs can be exploited to find selective drugs against infections such as malaria and leishmaniasis. Furthermore, G6PD is a prognostic marker in several cancer types and is also considered to be a tumour target. On the other hand, FGD is considered to be a target against Mycobacterium tuberculosis and possesses a high biotechnological potential in biocatalysis and bioremediation.

Porphyromonas gingivalis is a major pathogenic oral bacterium that is responsible for periodontal disease. It is linked to chronic periodontitis, gingivitis and aggressive periodontitis. P. gingivalis exerts its pathogenic effects through mechanisms such as immune evasion and tissue destruction, primarily by secreting various factors, including cysteine proteases such as gingipain K (Kgp), gingipain R (RgpA and RgpB) and PrtH (UniProtKB ID P46071). Virulence proteins comprise multiple domains, including the pro-peptide region, catalytic domain, K domain, R domain and DUF2436 domain. While there is a growing database of knowledge on virulence proteins and domains, there was no prior evidence or information regarding the structure and biological function of the well conserved DUF2436 domain. In this study, the DUF2436 domain of PrtH from P. gingivalis (PgDUF2436) was determined at 2.21 Å resolution, revealing a noncanonical β-jelly-roll sandwich topology with two antiparallel β-sheets and one short α-helix. Although the structure of PgDUF2436 was determined by the molecular-replacement method using an AlphaFold model structure as a template, there were significant differences in the positions of β1 between the AlphaFold model and the experimentally determined PgDUF2436 structure. The Basic Local Alignment Search Tool sequence-similarity search program showed no sequentially similar proteins in the Protein Data Bank. However, DaliLite search results using structure-based alignment revealed that the PgDUF2436 structure has structural similarity Z-scores of 5.9–5.4 with the C-terminal domain of AlgF, the D4 domain of cytolysin, IglE and the extracellular domain structure of PepT2. This study has elucidated the structure of the DUF2436 domain for the first time and a comparative analysis with similar structures has been performed.

The crystal structure of the intracellular domain of transforming growth factor β type I receptor (TβR1) in complex with the competitive inhibitor SB505124 is presented. The study provides insights into the structure and function of TβR1 in complex with SB505124, and as such offers molecular-level understanding of the inhibition of this critical signalling pathway. The potential of SB505124 as an avenue for therapy in cancer treatment is discussed on basis of the results.

Mevalonate kinase is central to the isoprenoid biosynthesis pathway. Here, high-resolution X-ray crystal structures of two mevalonate kinases are presented: a eukaryotic protein from Ramazzottius varieornatus and an archaeal protein from Methanococcoides burtonii. Both enzymes possess the highly conserved motifs of the GHMP enzyme superfamily, with notable differences between the two enzymes in the N-terminal part of the structures. Biochemical characterization of the two enzymes revealed major differences in their sensitivity to geranyl pyrophosphate and farnesyl pyrophosphate, and in their thermal stabilities. This work adds to the understanding of the structural basis of enzyme inhibition and thermostability in mevalonate kinases.

Human peptidylarginine deiminase isoform VI (PAD6), which is predominantly limited to cytoplasmic lattices in the mammalian oocytes in ovarian tissue, is essential for female fertility. It belongs to the peptidylarginine deiminase (PAD) enzyme family that catalyzes the conversion of arginine residues to citrulline in proteins. In contrast to other members of the family, recombinant PAD6 was previously found to be catalytically inactive. We sought to provide structural insight into the human homologue to shed light on this observation. We report here the first crystal structure of PAD6, determined at 1.7 Å resolution. PAD6 follows the same domain organization as other structurally known PAD isoenzymes. Further structural analysis and size-exclusion chromatography show that PAD6 behaves as a homodimer similar to PAD4. Differential scanning fluorimetry suggests that PAD6 does not coordinate Ca2+ which agrees with acidic residues found to coordinate Ca2+ in other PAD homologs not being conserved in PAD6. The crystal structure of PAD6 shows similarities with the inactive state of apo PAD2, in which the active site conformation is unsuitable for catalytic citrullination. The putative active site of PAD6 adopts a non-productive conformation that would not allow protein–substrate binding due to steric hindrance with rigid secondary structure elements. This observation is further supported by the lack of activity on the histone H3 and cytokeratin 5 substrates. These findings suggest a different mechanism for enzymatic activation compared with other PADs; alternatively, PAD6 may exert a non-enzymatic function in the cytoplasmic lattice of oocytes and early embryos.

Light–oxygen–voltage (LOV) domains are small photosensory flavoprotein modules that allow the conversion of external stimuli (sunlight) into intra­cellular signals responsible for various cell behaviors (e.g. phototropism and chloro­plast relocation). This ability relies on the light-induced formation of a covalent thio­ether adduct between a flavin chromophore and a reactive cysteine from the protein environment, which triggers a cascade of structural changes that result in the activation of a serine/threonine (Ser/Thr) kinase. Recent developments in time-resolved crystallography may allow the activation cascade of the LOV domain to be observed in real time, which has been elusive. In this study, we report a robust protocol for the production and stable delivery of microcrystals of the LOV domain of phototropin Phot-1 from Chlamydomonas reinhardtii (CrPhotLOV1) with a high-viscosity injector for time-resolved serial synchrotron crystallography (TR-SSX). The detailed process covers all aspects, from sample optimization to data collection, which may serve as a guide for soluble protein preparation for TR-SSX. In addition, we show that the crystals obtained preserve the photoreactivity using infrared spectroscopy. Furthermore, the results of the TR-SSX experiment provide high-resolution insights into structural alterations of CrPhotLOV1 from Δt = 2.5 ms up to Δt = 95 ms post-photoactivation, including resolving the geometry of the thio­ether adduct and the C-terminal region implicated in the signal transduction process.

Emily shares her experience research on Henslow’s sparrow accounting for the future effects of climate change and to develop risk assessment tools to assist managers in the region with meeting their conservation objectives using prescribed fire.

NASA released a workshop report on the UNBOUND-FEW workshop series, which was facilitated in part by Tribal Resilience Learning Network staff from the Alaska CASC. The workshop report reveals key recommendations for making data tools more useful for climate adaptation planning.

The Council of the National Academy of Sciences (NAS) is concerned about the confusion caused by a petition being circulated via a letter from a former president of this Academy.

Marijuanas active components are potentially effective in treating pain, nausea, the anorexia of AIDS wasting, and other symptoms, and should be tested rigorously in clinical trials.

As part of the $4 billion settlement announced today between the federal government and BP concerning the 2010 Deepwater Horizon disaster, the National Academy of Sciences has been asked to establish a new $350 million, 30-year program on human health and environmental protection in the Gulf of Mexico.

Today President Obama signed a Presidential Memorandum to address climate change and national security. The National Academies of Sciences, Engineering, and Medicine look forward to taking the lead in engaging the academic community in efforts to guide this initiative, and are well-positioned to tap the broad, multidisciplinary expertise of researchers across the nation.

Today during the National Academy of Sciences 154th annual meeting, in her first speech to the members of the Academy, NAS President Marcia McNutt stressed the ongoing vitality of America’s scientific enterprise, and called on the country to strengthen its support for science and to continue to turn to science for solutions to the nation’s and the world’s most pressing challenges.

NASA’s large strategic missions like the Hubble Space Telescope, the Curiosity rover on Mars, and the Terra Earth observation satellite are essential to maintaining the United States’ global leadership in space exploration and should continue to be a primary component of a balanced space science program that includes large, medium, and smaller missions, says a new report by the National Academies of Sciences, Engineering, and Medicine.

Although NASA has made progress toward the overall space exploration science priorities recommended in a 2011 decadal survey by the National Academies of Sciences, Engineering, and Medicine, the space agency should raise the priority of scientific research that addresses the risks and unknowns of human space exploration.

We are concerned deeply by a report that staff at the Centers for Disease Control and Prevention were instructed not to use certain words in budget documents. As leaders of the National Academies of Sciences, Engineering, and Medicine, we are especially stunned that “evidence-based” and “science-based” are reportedly among the barred terms.

The highest standards of scientific integrity, transparency, and accountability are critical to maintaining public confidence in our nation’s research enterprise and in the wise use of the public investment in research.

We are pleased to announce that the National Academies of Sciences, Engineering, and Medicine are exploring ways to mobilize our expertise to counter misinformation on the web related to science, engineering, and health.

NAS President Marcia McNutt, NAE President C. D. Mote, Jr., and NAM President Victor J. Dzau honored U.S. 2017 Nobel Prize recipients Barry Barish, Joachim Frank, Michael Rosbash, Kip Thorne, and Michael Young at a reception on Capitol Hill on Wednesday, April 18. U.S. Senators Lamar Alexander (R-Tenn.) and Christopher Coons (D-Del.) co-hosted the event.

From April 28 to May 1 the National Academy of Sciences will hold its 155th annual meeting.

During a ceremony at its 155th annual meeting, the National Academy of Sciences presented the 2018 Public Welfare Medal to physician, anthropologist, and humanitarian Paul Farmer.

The current process for planetary protection policy development is inadequate to respond to increasingly complex solar system exploration missions, says a new report from the National Academies of Sciences, Engineering, and Medicine.

I am pleased that the White House is moving to fill the position of director of the Office of Science and Technology Policy.

Despite significant cuts to NASAs Planetary Science Division budget early in this decade, the space agency has made impressive progress in meeting goals outlined in the 2013-2022 planetary decadal survey by the National Academies of Sciences, Engineering, and Medicine, says a new midterm assessment from the National Academies.

Read a new Perspective on promoting transparency in scientific authorship co-authored by NAS President Marcia McNutt. Appearing in the latest issue of Proceedings of the National Academy of Sciences, the piece offers suggestions for improving how author contributions are attributed in scientific publications.