Creating transgenic mosquitoes allows for mechanistic studies of basic mosquito biology and the development of novel vector control strategies. CRISPR–Cas9 gene editing has revolutionized gene editing, including in mosquitoes. This protocol details part of the gene editing process of Aedes aegypti mosquitoes via CRISPR–Cas9, through testing and validating single-guide RNAs (sgRNAs). Gene editing activity varies depending on the sequence of sgRNAs used, so validation of sgRNA activity should be done before large-scale generation of mutants or transgenics. sgRNA is designed using online tools and synthesized in <1 h. Once mutants or transgenics are generated via embryo microinjection, sgRNA activity is validated by quick genotyping polymerase chain reaction (PCR) and DNA sequencing.

Transposon-mediated transgenesis has revolutionized both basic and applied studies of mosquito vectors of disease. Currently, techniques such as enhancer traps and transposon tagging, which rely on remobilizable insertional mutagenesis, are only possible with transposon-based vector systems. Here, we provide general descriptions of methods and applications of transposon-based mosquito transgenesis. The exact procedures must be adapted to each mosquito species and comparisons of some differences among different mosquito species are outlined. A number of excellent publications showing detailed and specific protocols and methods are featured and referenced.

A group of volunteers have been providing emergency services to West Dawson residents during the periods of freeze up and break up, when there is no reliable access across the Yukon River to town.

In a new biopic starring Kate Winslet, Miller's many lives—as an artist, model, muse, cook and war correspondent—need little embellishment

When the U.S. Army massacred a Lakota village at Blue Water, dozens of plundered artifacts ended up in the Smithsonian. The unraveling of this long-buried atrocity is forging a path toward reconciliation

New Brunswick’s Court of Appeal has reserved judgment in the case of a man found guilty of criminal negligence in the death of Fredericton teenager in 2018.

Ginoogaming First Nation has declared a state of emergency after an engineering inspection showed the bridge leading to the community should be closed to traffic.

CBC's Superior Morning has been gathering wartime stories from listeners leading up to Remembrance Day based on mementos they have kept for generations. Here's some of the stories residents in northwestern Ontario have shared.

Buenos Aires, Argentina :: Miembros de la comunidad japonesa encuentran amigos en un barco de visita y escuchan el evangelio en su lengua materna.

Buenos Aires, Argentinen :: Technische Schiffsmitarbeiter geben Gottes Liebe an Schweißer vor Ort weiter, die bei der Reparatur der Logos Hope helfene

Jeff Monague has worked to incorporate Indigenous elements into Base Borden's Remembrance Day ceremonies — an acknowledgement he never had as a soldier.

At 2,700 square kilometres, CFB Suffield is the largest military training area in Canada. It has been the site of military training in southern Alberta since 1972.

A researcher in Grande Prairie, Alta., has undertaken a project to ensure past Indigenous soldiers and veterans are recognized for their sacrifices in both world wars.

Small businesses are keeping a close eye on the possibility of job action as they stock their shelves for Black Friday and holiday shopping.

The Town of Kensington, P.E.I., has committed to implementing a new campaign to end gender-based violence.

A federal audit of Canada’s strategy to promote the extraction of critical minerals says the government isn’t giving due consideration to the potential impact of mining on the environment, biodiversity and Indigenous people.

A new program to help fund the post-secondary education of Indigenous students in Manitoba will award 150 scholarships to train new health-care professionals. The Southern Chiefs Organization hopes this can go a long way in addressing health-care delivery and shorter life expectancy among First Nation communities.

Across Canada, there’s a growing number of political leaders proposing to force people into treatment for drug addiction, despite inconclusive evidence about its effectiveness.

These two Indigenous women veterans from Saskatchewan were able to serve in the U.S. Navy and Airforce because of the Jay Treaty, a 1794 agreement that allows some First Nations people to travel freely across the Canada-U.S. border for employment, study, retirement, investing and immigration.

Kimberly Murray has opened an uncomfortable and difficult but long overdue conversation about justice for Canada’s "disappeared" residential school children, Indigenous leaders say in response to the special interlocutor's two-volume final report.

The agenda for City Commission - Special Emergency Meeting dated Sunday, 26 April 2020 has been archived. Access it here:
https://oregon-city.granicus.com/MediaPlayer.php?view_id=4&clip_id=2415 Download File

The agenda for City Commission - Special Emergency Meeting dated Sunday, 07 June 2020 has been archived. Access it here:
https://oregon-city.granicus.com/MediaPlayer.php?view_id=4&clip_id=2430 Download File

The agenda for City Commission - Special Emergency Meeting dated Tuesday, 09 June 2020 has been archived. Access it here:
https://oregon-city.granicus.com/MediaPlayer.php?view_id=4&clip_id=2432 Download File

In his new book, former Maple Leafs captain Mats Sundin dives into the pressures and anxieties of being the first European drafted first overall to the NHL, getting traded by Quebec Nordiques to Toronto for Wendel Clark, and the turbulent end to his time with the team.

CBC Sports sat down with Hockey Hall of Famer Mats Sundin, showing him video of some of the biggest moments of his career.

A new course offered to nursing students at the University of Calgary is shining a light on the impacts of colonization on the health experiences of Indigenous peoples.

CRISPR–Cas9 has revolutionized gene editing for traditional and nontraditional model organisms alike. This tool has opened the door to new mechanistic studies of basic mosquito biology as well as the development of novel vector control strategies based on CRISPR–Cas9, including gene drives that spread genetic elements in the population. Although the promise of the specificity, flexibility, and ease of deployment CRISPR is real, its implementation still requires empirical optimization for each new species of interest, as well as to each genomic target within a given species. Here, we provide an overview of designing and testing single-guide RNAs for the use of CRISPR-based gene editing tools.

Transposon-mediated transgenesis of mosquito vectors of disease pathogens followed the early success of transgenesis in the vinegar fly, Drosophila melanogaster. The P transposable element used in Drosophila does not function canonically in mosquitoes, and repeatable, routine transgenesis in mosquitoes was not accomplished until new transposable elements were discovered and validated. A number of distinct transposons were subsequently identified that mediate the introduction of exogenous DNA in a stable and heritable manner in mosquito species, including members of the genera Aedes, Anopheles, and Culex. The most versatile element, piggyBac, is functional in all of these mosquito genera, as well as in many other insects in diverse orders, and has been used extensively outside the class. Transposon-mediated transgenesis of recessive and dominant marker genes and reporter systems has been used to define functional fragments of gene control sequences, introduce exogenous DNA encoding products beneficial to medical interests, and act as "enhancer traps" to identify endogenous genes with specific expression characteristics.

World's largest annual 3D CAD event will feature special guest Steve Wozniak this year

Competition sponsored by SOLIDWORKS and

U.K. company cut weeks out of design time, eliminated prototyping, and increased innovation with SOLIDWORKS and CircuitWorks

Equipment Designed and Modified With SolidWorks Software Located Miners and Helped Drill Escape Shaft Weeks Sooner Than Predicted

First SOLIDWORKS Application on the 3DEXPERIENCE Platform Accelerates Product Innovation on the Cloud

Reduces Time to Create Regulatory Reports While Increasing Data Accuracy

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report