Gram-negative bacteria possess an asymmetric outer membrane (OM) composed primarily of lipopolysaccharides (LPS) on the outer leaflet and phospholipids (PLs) on the inner leaflet. Loss of this asymmetry due to mutations in the lipopolysaccharide (LPS) biosynthesis or transport pathways causes externalization of PLs to the outer leaflet of the OM and leads to OM permeability defects. Here, we employed metabolic labeling to detect a compromised OM in intact bacteria. Phosphatidylcholine synthase (Pcs) expression in Escherichia coli allowed for incorporation of exogenous propargylcholine (PCho) into phosphatidyl(propargyl)choline (PPC) and for incorporation of exogenous 1-azidoethyl-choline (AECho) into phosphatidyl(azidoethyl)choline (AEPC) as confirmed by LC-MS analyses. A fluorescent copper-free click reagent poorly labeled AEPC in intact wild-type cells, but readily labeled AEPC from lysed cells. Fluorescence microscopy and flow cytometry analyses confirmed the absence of significant AEPC labeling from intact wild-type E. coli strains, and revealed significant AEPC labeling in an E. coli LPS transport mutant (lptD4213) and an LPS biosynthesis mutant (E. coli lpxC101). Our results suggest that metabolic PL labeling with AECho is a promising tool to detect a compromised bacterial OM, reveal aberrant PL externalization, and identify or characterize novel cell-active inhibitors of LPS biosynthesis or transport.

The Jamaica Defence Force (JDF) is promising "strong" action against soldiers found guilty of instructing violators of the national nightly COVID-19 curfew to engage in “corrective measures”. News of the allegations came in an...

Neoadjuvant chemotherapy for breast cancer is a new strategy that was introduced towards the end of the 20th century with the aim of reducing tumour size - rendering an otherwise inoperable tumour operable, allowing more conservative surgery, and hopefully improving overall survival. Although data indicate that the first rationale remains valid,...

A UK general election has been called - polling day is on the 12th of December, and from now until then we’re going to be bringing you a weekly election-themed podcast. We want to help you make sense of the promises and pledges, claims and counter-claims, that are being made around healthcare and the NHS out on the campaign trail. This week has...

A UK general election has been called - polling day is on the 12th of December, and from now until then we’re going to be bringing you a weekly election-themed podcast. We want to help you make sense of the promises and pledges, claims and counter-claims, that are being made around healthcare and the NHS out on the campaign trail. This week...

A UK general election has been called - polling day is on the 12th of December, and from now until then we’re going to be bringing you a weekly election-themed podcast. We want to help you make sense of the promises and pledges, claims and counter-claims, that are being made around healthcare and the NHS out on the campaign trail. This week...

UK general election has been called - polling day is on the 12th of December, and from now until then we’re going to be bringing you a weekly election-themed podcast. We want to help you make sense of the promises and pledges, claims and counter-claims, that are being made around healthcare and the NHS out on the campaign trail. This week we're...

The UK general election is happening this week, and you’ve probably made your mind up which MP you’re voting for already - and maybe the NHS has influenced that decision. This year has seen an increase in the number of doctors running for parliament, and in this podcast we find out what motivates doctors to step away from clinical practice, and...

4 December 2019

Given diverging demographics, rising educational attainment and wide variation in economic opportunities, countries in the Association of Southeast Asian Nations are poised to see an expansion of both the demand for and supply of skilled migrants willing and able to move. The convergence of these megatrends represents unique opportunities for human-capital development and brain circulation, as this report explores.

Anakinra, a drug developed to treat rheumatoid arthritis might help patients who have developed acute respiratory distress syndrome caused by COVID-19, a small study published by The Lancet Rheumatology has found.

The first bill introduced in the 116th Congress to offer a path to legal status to DREAMers, the American Dream and Promise Act of 2019, could legalize nearly 2.7 million unauthorized immigrants brought to the United States as children, as well as those eligible for Temporary Protected Status or Deferred Enforced Departure, as this commentary explains.

Durham, [NC] : Duke University Press, 2010.

National Association of Wine Retailers Release Letter Delivered to Attorneys General and Alcohol Regulatory Chiefs Concerning Unconstitutional and Unenforceable Wine Shipping Bans

(PRWeb April 15, 2020)

Read the full story at https://www.prweb.com/releases/wine_retailers_seek_alcohol_shipping_compromise_with_18_states/prweb17050617.htm

A chemical-safety bill in the Senate shows the wisdom of “good, old-fashioned legislating.”

Fewer than one-third of America's teachers say ed-tech innovations have changed their beliefs about what school should look like, according to a new Education Week survey.

It's resulted in a creepy Orwellian environment where Google, Facebook, Amazon, and others have replaced Big Brother.

A new plan from Democratic presidential candidate and former special educator Elizabeth Warren touches on some glaring issues in special education: graduation disparities, hard-to-access school buildings, and discipline practices that disproportionately affect black, Latino, and Native American stud

A new supercapacitor based on manganese oxide could combine the storage capacity of batteries with the high power and fast charging of other supercapacitors, according to researchers at Penn State and two universities in China.

Fly your DJI drone using a first-person perspective up to a range of 2.5 miles and race with up to seven other drones using the same low-latency system.

Bajaj has used it’s existing Pulsar 150 platform to create the smaller Pulsar 125 Neon. The design, cycle parts, features and even the engine (stroke has been shortened and swept volume reduced) is borrowed from the bigger bike. The result is a good looking bike for those on a budget. How is it to ride […]

Force T1N passenger van will go into production later this year and will be priced around Rs 25 lakh. It is an 18-seater and will be exported as well.

In a statement posted on the body's Twitter handle, IOA Secretary General Rajeev Mehta said "we are deeply grateful to all the front-line workers who are risking their lives to fight this pandemic."

A little bit of everything in the blog today: PSS is All Over As someone that was involved with UVM and PSS, both becoming Accellera standards, it is exciting to see both growing independently and together. With PSS we had a massive amount of papers ...(read more)

Whitepaper called From Zero Credentials to Full Domain Compromise. This paper covers techniques penetration testers can use in order to accomplish an initial foothold on target networks and achieve full domain compromise without executing third party applications or reusing clear text credentials.

Germany-based clean-energy fund Hep Kapitalverwaltung AG agreed to invest $50 million to $80 million annually in a partnership to develop small solar projects in the U.S.

One of the first unbiased, major comprehensive reviews of blockchain has concluded that the technology is “actually delivering on its promises in a number of areas directly related to energy”.

Offshore wind development is being pushed further out into deeper waters, emphasizing longer, higher-capacity transmission systems. Most newer offshore wind farms from Europe to the U.S. are looking at hundreds of kilometers of transmission lines: the U.K. Crown Estate's Round 3 allocations, interconnection systems from Germany's North Sea to the U.K.'s National Grid Western Link, and the proposed Atlantic Wind Connector in the U.S. Mid-Atlantic.

Cleantech investing has taken quite a hit in recent years. Last year, CBS News highlighted the “cleantech crash” on U.S. primetime television, and Bloomberg New Energy Finance, a Bloomberg-owned energy data firm, has tracked the multi-year decline in cleantech investing. However, there are additional trends that tell another side of the cleantech story and suggest innovation and hope for a low-cost, low-carbon future are far from gone.

With its revival for fiscal year 2014, the Hydroelectric Production Incentives Program shows promise as a new federal revenue stream for hydro facilities.

Check here each week to keep up with the latest from John MacArthur's pulpit at Grace Community Church.

Check here each week to keep up with the latest from John MacArthur's pulpit at Grace Community Church.

Check here each week to keep up with the latest from John MacArthur's pulpit at Grace Community Church.

Deepak Abbhi v Richard John Slade (trading as Richard Slade & Co) [2019] EWCA Civ 2175 The Court of Appeal recently unanimously held that an oral promise by a son-in-law to give money to his father-in-law to pay legal fees was an indemnity and n...

Encouraging settlement of employment disputes outside of the courts and tribunal system remains the cornerstone of employment legislation.  It also remains the case that the vast majority of parties resolve employment disputes without the ne...