We report a systematic, cellular phenotype-based antimalarial screening of the Medicines for Malaria Venture Pathogen Box collection, which facilitated the identification of specific blockers of late-stage intraerythrocytic development of Plasmodium falciparum. First, from standard growth inhibition assays, we identified 173 molecules with antimalarial activity (50% effective concentration [EC₅₀] ≤ 10 μM), which included 62 additional molecules over previously known antimalarial candidates from the Pathogen Box. We identified 90 molecules with EC₅₀ of ≤1 μM, which had significant effect on the ring-trophozoite transition, while 9 molecules inhibited the trophozoite-schizont transition and 21 molecules inhibited the schizont-ring transition (with ≥50% parasites failing to proceed to the next stage) at 1 μM. We therefore rescreened all 173 molecules and validated hits in microscopy to prioritize 12 hits as selective blockers of the schizont-ring transition. Seven of these molecules inhibited the calcium ionophore-induced egress of Toxoplasma gondii, a related apicomplexan parasite, suggesting that the inhibitors may be acting via a conserved mechanism which could be further exploited for target identification studies. We demonstrate that two molecules, MMV020670 and MMV026356, identified as schizont inhibitors in our screens, induce the fragmentation of DNA in merozoites, thereby impairing their ability to egress and invade. Further mechanistic studies would facilitate the therapeutic exploitation of these molecules as broadly active inhibitors targeting late-stage development and egress of apicomplexan parasites relevant to human health.

If you're not too busy swinging through the streets, perfecting your armour, or exploring the Dreaming City we've got a new RPG Cast for you. And if the current fall lineup wasn't already overwhelming, then get ready for a long list of new titles announced or shown recently that are going to make you wonder how you can ever get caught up.

As a biological anthropologist focused on health, diseases are part of Sabrina Sholts' specialty

Hedgehogs have indulging in "noisy lovemaking" during the coronavirus lockdown, according to an expert,

Total War: Shogun 2 is FREE on Steam right now!
https://store.steampowered.com/app/201270/Total_War_SHOGUN_2/

Hulk Hogan (66) has had 17 surgeries over the past couple of decades, 10 of them on his back only with his “Immortal” tattoo on top. The first time he went under the knife was back in 2009 after suffering extreme back pain. Now, he decided to share with the world his battle scars. “I’ve […]

The post Hulk Hogan Shows Off His Battle Scars appeared first on Chart Attack.

Inside the cast read through for NEMESIS from Shogun Films * The read through took place before the Covid-19 restrictions came into force and before the requirement for social distancing. Shogun Films, the disruptive new British indie film start up from industry vet Jonathan Sothcott (We Still Kill The Old Way) was just a week […]

The post ‘NEMESIS’ – From Shogun Films appeared first on UKFilmNews.com.

Renowned Northern Irish star's stage and screen career spanned four decades

The actor was been known for starring in the BBC Northern Ireland comedy series Give My Head Peace

The Justice Department announced today a settlement with Cuyahoga County, Ohio, to protect the rights of Spanish-speaking Puerto Rican voters under Section 4(e) of the Voting Rights Act.

Freedman Farms, Inc. and its president, William B. Freedman, pleaded guilty yesterday in federal court in New Bern, N.C., to violating the Clean Water Act when they discharged hog waste into a stream that leads to the Waccamaw River.

Freedman Farms Inc. was sentenced today in federal court to five years probation and ordered to pay $1.5 million in fines, restitution and community service payments for violating the Clean Water Act when they discharged hog waste into a stream that leads to the Waccamaw River.

Perhaps the most shocking episode of repression in Saudi Arabia’s recent history is the brutal and bizarre murder of Jamal Khashoggi, a U.S. resident and columnist for the Washington Post, in the Saudi consulate in Istanbul in October 2018. Two weeks ago, the United Nations Special Rapporteur on extrajudicial, summary or arbitrary executions, Agnes Callamard,…

It's déjà vu all over again, with the old guard trying to stop the newcomers...

Wiarton Willie saw his shadow this morning, which means the cold weather will stick around.

Forty years ago, activists blocked plans to dam the Buffalo River, eventually getting Congress and President Nixon to designate the river as America's first National River. Now, a factory hog farm is putting one of Arkansas' most beautiful spots at risk.

Potty Talk

Potty Talk

Consejos para el Hogar

Transforma Tu Hogar para este Verano

"I hope it's not that bad, but we should assume it will be until we know otherwise," Gates wrote in an article published Friday in The New England Journal of Medicine.

photo taken March 2020

For a while supper and wine were sufficient; now I’m watching every adaptation that is better than its source material

I suspect I’m not alone in this but, at some point in the past two weeks, I hit my lockdown wall. Not literally, although apparently the “banging one’s head against the kitchen wall” phase kicks in on the eighth week, so that’s something to put in the diary. But last week I felt really, really over it. Enough with every day being the bloody same; enough with watching my children become increasingly fretful because they haven’t seen their friends in over a month, the equivalent of five years to a pair of four-year-olds. But unless you want to be one of those delightful people protesting the lockdown in the US, clothed in stars and stripes, AK-47s across their backs, what choice do we have? So, like Bill Murray, we grind out the same day, again and again and again.

The trick is to invent things to look forward to. For a while, “supper” and “wine” were sufficient, but repetition has dulled their efficacy. So I set myself challenges, driven on by the thrill of completion. Some people hear the word “challenge” and think, “Fitness!” Those people are not me. “Rewatch the entirety of 30 Rock” is more my speed. It is so soothing to watch a show about a luxuriantly bouffanted New York tycoon who isn’t a moron. In a just world, Jack Donaghy would be the US president instead of, well, you get the point. Then, sparked by his brilliant turn as Chris Tarrant on the ITV drama, Quiz, my next challenge was, “Watch every Michael Sheen performance in which he plays a real person”. This was deeply enjoyable, even if, in my lockdown-confused mind, I now think Brian Clough interviewed Richard Nixon on TV and Kenneth Williams was prime minister when Diana died.

Peri Peri Twister. Pics/Sameer Markande

The led lights fitted in 3D wall panels engulf The Pabulum (Latin: food) with a violet glow that spills on to the street. Standing next to a chemist and a stone's throw away from a farsan store, the QSR off SV Road in Santacruz, which promises 'fiery fries' and 'sexy shakes', resembles an alien spaceship.

The counter has a slot that holds these BBQ Cheese Fries served in a conical container

Our analogy isn't far off the mark as we step in and overhear a middle-aged couple, curious to know what Nacho Fries and Cheesy Potato Twisters actually mean. A millennial helming the service station explains the dishes. He points to staffers working in a bot-like fashion, adding dollops of Mexican salsa to golden fries and slicing potatoes to create spirals on a stick in an open kitchen. The couple places the order and moves to one of the two dining counters in the space, launched last month by three foodpreneurs - 21-year-olds Himanshu Jain and Mayank Jain, and their friend Deepak Joshi, 26, who runs a catering business.

A staffer preps the fries in the kitchen

The menu is The J-meets-Keventers-meets-Irla's Twisted Potato. It features French fries served with a host of vegetarian toppings - from aioli garlic to tandoori mayo and Schezwan sauce. They also offer Jain fries made with raw banana. From blueberry and rose to popcorn and Parle G, an array of ingredients make their way into the shakes, along with four varieties of twisters.

Death By Chocolate

A-peeling twist
We begin with the Peri Peri Twister ('69), a 12-inch long tornado of spicy flavours. The potato spiral - deep-fried and crisp but non-greasy - is piped with a fiery, paprika-and-garlic infused mayonnaise instead of a powdered spice mix. This enhances the stick, where the mayo's slight tang balances the heat. We wash it down with Popcorn Caramel Shake ('149), a lip-smacking, creamy rendition of the munchie with just the right hint of burnt brown sugar.

Popcorn Caramel Shake

The BBQ Cheese Fries ('99) feature crisp-till-golden, starchy potato fries doused with a peppery, slightly sweet barbeque sauce with an oh-so-good smoked taste that balances the sharpness of the cheese sauce. Our friend remarks, "They are better than the ones at The J."

The Poppers & Fries ('149) comprise three deep-fried cheese balls perched on a bed of fries, doused in chilli garlic sauce and mayonnaise. While the tangy-spicy sauces complement the dish, the poppers are underwhelming, and stodgy.

Try a dessert fry
Heading for a carb crash by now, we pick the apt climax - Death By Chocolate ('149). Coated with generous amounts of Nutella and chocolate sauce, the crisp, unsalted fries, topped with grated cheese, make for a great dessert. We exit with a promise to return, once we're cured of a potato hangover.

Time: 11 am to 11 pm
At: Shop No 3, Tagore Kunj, Saraswati Road, Santacruz West
Call: 8080805775 (delivery via Swiggy and Zomato)

28/02/2020

Face masks may protect Livestock workers like those who work on hog farms from a resistant form of staph bacteria. Even the family members of these farmers

The coronavirus that originated from Wuhan, China has already claimed the lives of more than 253,230 people worldwide.

Lean Hogs prices surged past $6.5 per pound in May, a level not seen since late-March, as strong demand in China, the world largest consumer of pork, combined with supply disruptions from the coronavirus epidemic pushed prices higher. The supply shortage would remain even after the country's economy recover from the pandemic. China pork production has dropped more than a fifth, as African swine fever, a viral disease of domestic and wild pigs, hit the world's top hog market last year. Historically, Lean Hogs reached an all time high of 133.80 in July of 2014. The Lean Hogs market prices displayed in Trading Economics are based on over-the-counter (OTC) and contract for difference (CFD) financial instruments. Our Lean Hogs market prices are intended to provide you with a reference only, rather than as a basis for making trading decisions. Trading Economics does not verify any data and disclaims any obligation to do so.

We need to get acclimatised to repetition. Many of us have not internalised the arduous road ahead

Good TV deserves a snack upgrade

It was a rare date night for busy couple - who haven't made a public appearance together in months - but they looked loved-up as they went along to the Harry Potter event on Thursday.

Wing Seymour (rib) and utility back Kinghorn (ankle) both sustained injuries in Saturday's defeat to Wales and join full-back Hogg on the sidelines, who has damaged his shoulder.

Hogg said he would be 'very annoyed' if one of his Scotland team-mates was so quick to move on from their own exit at this year's showpiece in Japan.

Stuart Hogg admits that if his Exeter Chiefs side win the Premiership, people will believe they triumphed only because of Saracens' hefty points deduction for breaching the salary cap.

Exeter Chiefs are desperate for their 'normal' squad to solve the European puzzle this year, having added Border piece Stuart Hogg. The 27-year-old Hawick-born full-back faces his old club.

CHRIS FOY AT THE AJ BELL STADIUM: Exeter extended their lead at the top of Pool Two of the Heineken Champions Cup with a close-fought 22-20 victory over Sale courtesy of Stuart Hogg (middle).

New Scotland captain Stuart Hogg is to seek out national icon Gavin Hastings for last-minute advice on how to lead the team from full-back in the Six Nations opener against Ireland.

The headlines will have made painful reading for Stuart Hogg yesterday after his costly howler in Dublin, but I thought he fronted up well afterwards and stayed focused during the game.

Scotland head coach Gregor Townsend has backed captain Stuart Hogg to bounce back from his horror miss against Ireland and lead the team with confidence against England next week.

Gregor Townsend has absolved Stuart Hogg from blame despite the Scotland skipper making his second costly mistake in as many Guinness Six Nations games.

ROB ROBERTSON IN ROME: Scotland head coach Gregor Townsend believes the pressure has been lifted after his side 'zeroed' Italy in an emphatic Six Nations win in the Italian capital.

SARAH VINE: When I saw the picture posted online by Sky News sports presenter Jacquie Beltrao of her son's bedroom, I couldn't resist a wry smile.

Tributes have been paid to Game Of Thrones actor BJ Hogg following his death aged 65.

Hogg said he would be 'very annoyed' if one of his Scotland team-mates was so quick to move on from their own exit at this year's showpiece in Japan.