A home repair show that helps Coloradans recover from shady contractors is accused of hiring just such a contractor for a project in Larkspur.

[Written by Stephen Wright] Whale researcher Andrew Stevenson will screen his two award-winning documentaries, ‘Where the Whales Sing’ and ‘The Secret Lives of the Humpbacks’ at the Speciality Cinema and Grill in Hamilton on Monday [December 4]. It will be the first time both documentaries are screened back-to-back, starting at 6.30 pm, with a 15-minute […]

Five years of data collected on reefs and offshore in Bermuda shows that coral reef chemistry – and perhaps the future success of corals – is tied not only to the human carbon emissions causing systematic ocean acidification, but also to seasonal and decadal cycles in the open waters of the Atlantic, and the balance […]

Camiko Smith leads the Butterfield Bermuda Championship local qualifier after the first round at a rain soaked Port Royal Golf Course. Smith shot a round of 3-under par 68 to lead by four strokes over a three-wat tie for second place between Oliver Betschart, Damian Palanyandi, and Adam Perry who all shot a 1-over par […]

[Written by Stephen Wright] Three fighters from Fight City Muay Thai and Fitness have returned to the island with silver medals from the Tournament of Champions at the Arnold Sports Festival in Columbus, Ohio, last week. Ashton Bell, Ashleigh Mayo, and debutant Trevor Smith claimed silver in their respective divisions at the annual tournament. Bell […]

[Written by Stephen Wright] Nine athletes from the Bermuda Sanshou Association will hope to be in the medal hunt at the Phoenix Wushu Nationals in Phoenix, Arizona, this weekend. Krista Dyer, Kallan Todd, Quennel Robinson, Enzi Johnston, Muryah Swan, Geraldine Sidders, Tristan Robinson, and Jonathan Trott will compete in sanda [Chinese kickboxing]. Kaelin Cox is […]

Premier David Burt and his family released their annual Cup Match photo, featuring clothing by Bermudian designer, business owner and creative Jordan Carey, founder and Creative Director of the Loquat brand. A spokesperson said, “Premier David Burt, JP, MP and his family have today shared their annual Cup Match photo. “This year’s official photoshoot is particularly […]

Team Bermuda will dazzle today [July 26] at the Paris Olympics Opening Ceremony, as the Bermuda Olympic Association announces local fashion designers Courtney Clay, Channing Dill, and Alana Wade as the official designers for the 2024 Olympic Games. A spokesperson said, “Clay is credited with the custom scarf, tie, and pocket squares. Dill created a […]

[Updated with video] The Bermuda Tourism Authority [BTA] unveiled its “Paint the Town Pink” initiative which features artwork from three Bermuda artists — Nhuri Bashir, Alshanté Foggo, and Shanna Hollis — in various locations in New York City. A spokesperson said, “The Bermuda Tourism Authority [BTA] has unveiled its “Paint the Town Pink” initiative, a campaign […]

Back in June, we enjoyed seeing a lot of birds, butterflies, and moths in Greece. Many, if not most, of those were species rarely, if ever, seen in the UK. One bird we had a shout out from our skipper on Lake Kerkini was the Purple Heron, Ardea purpurea. I must confess I wasn’t quick … Continue reading "Local Purple Heron, Ardea purpurea"

1. Legitimacy of two Victorian local government elections in question after duplicate votes detected  ABC News
2. Victorian council election results 2024 LIVE updates: Suspected postal vote tampering in council elections  Sydney Morning Herald
3. VEC investigates potential vote tampering in two Melbourne councils  The Age

With the economy finally starting to gain some traction, few people are thinking about those whom society is le aving behind. These are the working poor, the sick and the elderly. As the wars in Iraq and Afghanistan wind down, we will see more soldiers and their families in need.

Sometimes fundraising calls for a national effort. The only way to gather money and resources quickly is by calling or mailing into every state for support. This is a proven method of soliciting money. This isn't the case for every fundraising effort however.

There are some issues that could just as well be taken care of locally. Additionally, some causes are actually better served by using local fundraisers. Local has a number of benefits; we'll name a few below.

More of the money goes to the cause.
Some national fundraising companies can take as much as 80% of what they raise for an organization. Millions of dollars are wasted in this way. Local groups just can't afford to pay these prices. A local campaign, whether staffed by volunteers or local professionals, ensures that more of the donated dollars go to work immediately.

No one knows local issues like local people.
Using locals to fund raise means having a team that understands the issue and is passionate about it. The more knowledgeable and interested in an issue a fundraiser is, the more likely they are to secure donations.

As a nation, a number of important issues are affecting Americans;
children going to bed hungry, seniors and veterans not getting the care that they deserve and homelessness are just a few of these issues. What all of these issues all have in common is that they begin and end locally.

We can choose to wait for a solution from Washington, but President Obama's job forces him to look at the big issues, not the small ones. National organizations face similar problems; they have the money and resources, but not the organization to implement relief locally. Local fundraisers raising and spending money locally have an immediate effect on communities.

Bringing the idea of raising money in the community and solving problems locally is one whose time has come. More local groups are starting to see the power of collecting donations at home. As the idea spreads, the benefits will only become greater.

DOGE trades at premium on Upbit and Bithumb relative to Binance. - The price differential is still noticeably lower than the previous bull market peaks. Today is just that day as Koreans seem to be jumping into the DOGE market, driving prices higher and creating a noticeable premium on local…

Okay, Hopefully you are Awake.  The article is for sure helpful, especially if you are looking to increase your SEO Local presence.  Some simple strategies for finding out more about your competition is to just simply locate the competitors on the local 7-Pack listing in the results and copy and past the address in the […]

This research was undertaken by Stuart Fordyce as part of an MSc in Integrated Service Improvement (Health and Social Care) at the University of Edinburgh. It considers the impact of a rapidly ageing population on a Scottish local authority and its attempt to shift the focus to a more contemporary service provision. The aim is to explore what factors are inhibiting the effectiveness of enablement. Using a case study approach the research explored whether: (i) enablement is wholly effective in addressing the increase in current service demands; (ii) the approach adopted by the local authority is undermined as services are now over extended and are attempting to address competing objectives, and; (iii) the paradigmatic change across the sector in the future will materialise if organisations cannot create the capacity and infrastructure to enable change to occur

People are paying more attention to what they eat right now than at any other point in history. There's a lot more focus on organic ingredients, fresh ingredients and locally-sourced ingredients. At the famous Steirereck restaurant, you are surrounded by farmlands where the ingredients in your food are grown. Now, PPAG Architects have completed Sterock am Pogusch. This is an offshoot of Steirereck and it will take you into the Austrian Alps.[...]

Whoever says fast food is unhealthy for the body and the environment hasn’t been introduced to Sweetgreen, a chain restaurant dedicated to changing how we view food production, consumption and waste. [...]

A teen in Canada may be the first person to catch an H5 bird flu virus within the country. Health officials are now working to confirm the diagnosis.

We have updated the list of the Amazon local stores. Several more locales were added to the Location option in the Advanced settings of our Amazon Feeds. At the moment we have 20 local Amazon stores on the list: United States Canada United Kingdom Australia Mexico Brazil Germany France Italy Spain Sweden Netherlands Poland Turkey […]

The post New Amazon Local Stores Added appeared first on RSSground.com.

Students across the country are participating in NOVA's film production program to make videos about climate change solutions in their local communities.

Bolster learning for middle and high school students about the myriad ways our weather is changing, how communities are being impacted, and innovative solutions.

Three STEM educators share best practices for tackling climate change in the classroom through project-based learning.

As a digital company, your business is not bound by conventional borders and has the freedom and opportunity to reach customers anywhere in the world. And just as you look outward for new markets, customers are also looking beyond their physical borders for their goods and services. As a study by KPMG shows, online buyers […]

The South African Artificial Intelligence Association wants LinkedIn to be investigated, as it claims the social networking platforms new data use practice violates local personal information protection law.

Saverio Cinti
Nov 1, 2005; 46:2347-2355
Research Articles

From local to global: A roadmap for US climate action 14 April 2021 — 6:30PM TO 8:00PM Anonymous (not verified) 28 March 2021 Online

Ahead of Biden’s Earth Day Summit, panellists discuss a range of climate issues, from city-level climate management to the international security implications of climate deals.

On their first day in office, the Biden-Harris administration sent a strong message to Americans and allies by rejoining the Paris Agreement. Experienced climate and environmental leaders were appointed to senior leadership roles as part of a ‘whole of government approach’ to climate action.

Although decisive action is welcomed by many Americans and international partners, the divided domestic perspectives on climate and a changed international landscape pose significant challenges.

Ahead of the Earth Day Summit on April 22, an event hosted by Joe Biden to mark America’s formal return to global climate talks, panellists discuss a range of climate issues, from city-level climate management to the international security implications of climate deals.

• How will post-COVID domestic priorities and policy influence the international approach of the US to climate action?
• How will US policy, both foreign and domestic, need to respond to the security and geopolitical elements posed by climate change?
• What actions are needed during the upcoming Earth Day Summit for the US to establish credibility as a climate leader?
• What shape are key debates taking on US-China climate relations ahead of COP 26, and how might climate issues be approached in relation to wider geopolitical tensions?

Constantin Carle and Marlis Hochbruck
Math. Comp. 93 (), 2611-2641.
Abstract, references and article information

Lasse L. Wolf and Hong-Wei Zhang
Proc. Amer. Math. Soc. 152 (), 5445-5453.
Abstract, references and article information

Yong Suk Moon
Proc. Amer. Math. Soc. 152 (), 5095-5103.
Abstract, references and article information

Visual Abstract

The subcellular localization of Arf family proteins is generally thought to be determined by their corresponding guanine nucleotide exchange factors. By promoting GTP binding, guanine nucleotide exchange factors induce conformational changes of Arf proteins exposing their N-terminal amphipathic helices, which then insert into the membranes to stabilize the membrane association process. Here, we found that the N-terminal amphipathic motifs of the Golgi-localized Arf family protein, Arfrp1, and the endosome- and plasma membrane–localized Arf family protein, Arl14, play critical roles in spatial determination. Exchanging the amphipathic helix motifs between these two Arf proteins causes the switch of their localizations. Moreover, the amphipathic helices of Arfrp1 and Arl14 are sufficient for cytosolic proteins to be localized into a specific cellular compartment. The spatial determination mediated by the Arfrp1 helix requires its binding partner Sys1. In addition, the residues that are required for the acetylation of the Arfrp1 helix and the myristoylation of the Arl14 helix are important for the specific subcellular localization. Interestingly, Arfrp1 and Arl14 are recruited to their specific cellular compartments independent of GTP binding. Our results demonstrate that the amphipathic motifs of Arfrp1 and Arl14 are sufficient for determining specific subcellular localizations in a GTP-independent manner, suggesting that the membrane association and activation of some Arf proteins are uncoupled.

Hideaki Kuge
Dec 1, 2020; 61:1747-1763
Research Articles

Michael E. Abrams
Dec 1, 2020; 61:1538-1538
Images in Lipid Research

Jenny E. Kanter
Dec 8, 2020; 0:jlr.ILR120001217v1-jlr.ILR120001217
Images in Lipid Research

Human genetic studies recently identified an association of SNPs in the 17-β hydroxysteroid dehydrogenase 13 (HSD17B13) gene with alcoholic and nonalcoholic fatty liver disease development. Mutant HSD17B13 variants devoid of enzymatic function have been demonstrated to be protective from cirrhosis and liver cancer, supporting the development of HSD17B13 as a promising therapeutic target. Previous studies have demonstrated that HSD17B13 is a lipid droplet (LD)-associated protein. However, the critical domains that drive LD targeting or determine the enzymatic activity have yet to be defined. Here we used mutagenesis to generate multiple truncated and point-mutated proteins and were able to demonstrate in vitro that the N-terminal hydrophobic domain, PAT-like domain, and a putative α-helix/β-sheet/α-helix domain in HSD17B13 are all critical for LD targeting. Similarly, we characterized the predicted catalytic, substrate-binding, and homodimer interaction sites and found them to be essential for the enzymatic activity of HSD17B13, in addition to our previous identification of amino acid P260 and cofactor binding site. In conclusion, we identified critical domains and amino acid sites that are essential for the LD localization and protein function of HSD17B13, which may facilitate understanding of its function and targeting of this protein to treat chronic liver diseases.

The plasma membrane of neurons consists of distinct domains, each of which carries specialized functions and a characteristic set of membrane proteins. While this compartmentalized membrane organization is essential for neuronal functions, it remains controversial how neurons establish these domains on the laterally fluid membrane. Here, using immunostaining, lipid-MS analysis and gene ablation with the CRISPR/Cas9 system, we report that the pancreatic lipase-related protein 2 (PLRP2), a phospholipase A1 (PLA1), is a key organizer of membrane protein localization at the neurite tips of PC12 cells. PLRP2 produced local distribution of 1-oleoyl-2-palmitoyl-PC at these sites through acyl-chain remodeling of membrane phospholipids. The resulting lipid domain assembled the syntaxin 4 (Stx4) protein within itself by selectively interacting with the transmembrane domain of Stx4. The localized Stx4, in turn, facilitated the fusion of transport vesicles that contained the dopamine transporter with the domain of the plasma membrane, which led to the localized distribution of the transporter to that domain. These results revealed the pivotal roles of PLA1, specifically PLRP2, in the formation of functional domains in the plasma membrane of neurons. In addition, our results suggest a mode of membrane organization in which the local acyl-chain remodeling of membrane phospholipids controls the selective localization of membrane proteins by regulating both lipid-protein interactions and the fusion of transport vesicles to the lipid domain.

Renal Cell Carcinoma (RCC) is one of the most commonly diagnosed cancers worldwide with research efforts dramatically improving understanding of the biology of the disease. To investigate the role of the immune system in treatment-naïve clear cell Renal Cell Carcinoma (ccRCC), we interrogated the immune infiltrate in patient-matched ccRCC tumor samples, benign normal adjacent tissue (NAT) and peripheral blood mononuclear cells (PBMCs isolated from whole blood, focusing our attention on the myeloid cell infiltrate. Using flow cytometric, MS, and ExCYT analysis, we discovered unique myeloid populations in PBMCs across patient samples. Furthermore, normal adjacent tissues and ccRCC tissues contained numerous myeloid populations with a unique signature for both tissues. Enrichment of the immune cell (CD45⁺) fraction and subsequent gene expression analysis revealed a number of myeloid-related genes that were differentially expressed. These data provide evidence, for the first time, of an immunosuppressive and pro-tumorigenic role of myeloid cells in early, clinically localized ccRCC. The identification of a number of immune proteins for therapeutic targeting provides a rationale for investigation into the potential efficacy of earlier intervention with single-agent or combination immunotherapy for ccRCC.

Neoadjuvant therapy in patients with locally advanced rectal cancer (LARC) has achieved good pathologic complete response (pCR) rates, potentially eliminating the need for surgical intervention. This study investigated preoperative methods for predicting pCR after neoadjuvant short-course radiotherapy (SCRT) combined with immunochemotherapy. Methods: Treatment-naïve patients with histologically confirmed LARC were enrolled from February 2023 to July 2023. Before surgery, the patients received neoadjuvant SCRT followed by 2 cycles of capecitabine and oxaliplatin plus camrelizumab. ⁶⁸Ga-labeled fibroblast activation protein inhibitor ([⁶⁸Ga]Ga-FAPI-04) PET/MRI, [¹⁸F]FDG PET/CT, and contrast-enhanced MRI were performed before treatment initiation and before surgery in each patient. PET and MRI features and the size and number of lesions were also collected from each scan. Each parameter’s sensitivity, specificity, and diagnostic cutoff were derived via receiver-operating-characteristic curve analysis. Results: Twenty eligible patients (13 men, 7 women; mean age, 60.2 y) were enrolled and completed the entire trial, and all patients had proficient mismatch repair or microsatellite-stable LARC. A postoperative pCR was achieved in 9 patients (45.0%). In the visual evaluation, both [⁶⁸Ga]Ga-FAPI-04 PET/MRI and [¹⁸F]FDG PET/CT were limited to forecasting pCR. Contrast-enhanced MRI had a low sensitivity of 55.56% to predict pCR. In the quantitative evaluation, [⁶⁸Ga]Ga-FAPI-04 change in SUL_peak percentage, where SUL_peak is SUV_peak standardized by lean body mass, had the largest area under the curve (0.929) with high specificity (sensitivity, 77.78%; specificity, 100.0%; cutoff, 63.92%). Conclusion: [⁶⁸Ga]Ga-FAPI-04 PET/MRI is a promising imaging modality for predicting pCR after SCRT combined with immunochemotherapy. The SUL_peak decrease exceeding 63.92% may provide valuable guidance in selecting patients who can forgo surgery after neoadjuvant therapy.

Montana Superintendent Elsie Arntzen offers practical advice to schools that could open as early as May 7, even as she says "how they open schools and how learning takes place is up to them."

Some states, such as California, Kentucky and North Dakota plan to use the Every Student Succeeds Act to bolster the decision-making powers of their local school boards in the coming years.

The state will be allowed to use competency-based tests developed by local and state officials, trying them out with a handful of districts in lieu of statewide tests.

Washington lawmakers last week reached a deal to lift the state's "levy lid," blunting tighter limits on voter-approved local taxes for schools that were set to take effect this year. Gov. Jay Inslee, a Democrat, was expected to sign the legislation that Republicans had criticized.

FAO and China have signed a two-year tripartite cooperation agreement worth about N$10.5 million (US$1.5 million) that will boost the efforts of local farmers in Namibia. The agreement, which is [...]

Waterloo region’s food landscape is more than farm stands and restaurants. There's a vast array of food producers, food makers and organizations that make up the food scene. And now, a new directory aims to make them easier to find. CBC KW's food columnist Jasmine Mangalaseril explored the directory.

Here comes Santa Claus! There are a number of parades set to take place in November and December both during the day and in the evening. Here's a list of where you can find the big man in red.

The Town of Tecumseh will be getting six new EV charging stations, the province announced Tuesday, in a bid to "support the electrification of transportation across the province."