The government has given the nod for establishing COVID First-line Treatment Centres (CFTC) in the State as a contingency measure. The CFTC will be th

Interacting with locals, he observed that the situation is gradually improving day by day, and urged them not to pay attention to rumours and avoid circulating unconfirmed messages.

By now, everyone knows how to detect and use localStorage. However, I’ve been using a seemingly unconventional (but slightly more efficient) technique to do so. Since I haven’t seen it documented anywhere else, here goes!

Seven killed in protests over food distribution in Afghanistan, local MP says - Times of India

The makers of Yash's movie 'KGF' have planned to sue a local Telugu channel for broadcasting the movie illegally

The redetermination of the crystal structure of barium oxidotellurate(IV) monohydrate allowed the localization of the hydrogen atoms that were not determined in the previous study [Nielsen, Hazell & Rasmussen (1971). Acta Chem. Scand. 25, 3037–3042], thus making an unambiguous assignment of the hydrogen-bonding scheme possible. The crystal structure shows a layered arrangement parallel to (001), consisting of edge-sharing [BaO6(H2O)] polyhedra and flanked by isolated [TeO3] trigonal pyramids on the top and bottom. O—H⋯O hydrogen bonds of medium strength link adjacent layers along [001].

In this article, a method is presented to estimate a new local quality measure for 3D cryoEM maps that adopts the form of a `local resolution' type of information. The algorithm (DeepRes) is based on deep-learning 3D feature detection. DeepRes is fully automatic and parameter-free, and avoids the issues of most current methods, such as their insensitivity to enhancements owing to B-factor sharpening (unless the 3D mask is changed), among others, which is an issue that has been virtually neglected in the cryoEM field until now. In this way, DeepRes can be applied to any map, detecting subtle changes in local quality after applying enhancement processes such as isotropic filters or substantially more complex procedures, such as model-based local sharpening, non-model-based methods or denoising, that may be very difficult to follow using current methods. It performs as a human observer expects. The comparison with traditional local resolution indicators is also addressed.

Current software tools for the automated building of models for macro­molecular X-ray crystal structures are capable of assembling high-quality models for ordered macromolecule and small-molecule scattering components with minimal or no user supervision. Many of these tools also incorporate robust functionality for modelling the ordered water molecules that are found in nearly all macromolecular crystal structures. However, no current tools focus on differentiating these ubiquitous water molecules from other frequently occurring multi-atom solvent species, such as sulfate, or the automated building of models for such species. PeakProbe has been developed specifically to address the need for such a tool. PeakProbe predicts likely solvent models for a given point (termed a `peak') in a structure based on analysis (`probing') of its local electron density and chemical environment. PeakProbe maps a total of 19 resolution-dependent features associated with electron density and two associated with the local chemical environment to a two-dimensional score space that is independent of resolution. Peaks are classified based on the relative frequencies with which four different classes of solvent (including water) are observed within a given region of this score space as determined by large-scale sampling of solvent models in the Protein Data Bank. Designed to classify peaks generated from difference density maxima, PeakProbe also incorporates functionality for identifying peaks associated with model errors or clusters of peaks likely to correspond to multi-atom solvent, and for the validation of existing solvent models using solvent-omit electron-density maps. When tasked with classifying peaks into one of four distinct solvent classes, PeakProbe achieves greater than 99% accuracy for both peaks derived directly from the atomic coordinates of existing solvent models and those based on difference density maxima. While the program is still under development, a fully functional version is publicly available. PeakProbe makes extensive use of cctbx libraries, and requires a PHENIX licence and an up-to-date phenix.python environment for execution.

The performance of automated protein model building usually decreases with resolution, mainly owing to the lower information content of the experimental data. This calls for a more elaborate use of the available structural information about macromolecules. Here, a new method is presented that uses structural homologues to improve the quality of protein models automatically constructed using ARP/wARP. The method uses local structural similarity between deposited models and the model being built, and results in longer main-chain fragments that in turn can be more reliably docked to the protein sequence. The application of the homology-based model extension method to the example of a CFA synthase at 2.7 Å resolution resulted in a more complete model with almost all of the residues correctly built and docked to the sequence. The method was also evaluated on 1493 molecular-replacement solutions at a resolution of 4.0 Å and better that were submitted to the ARP/wARP web service for model building. A significant improvement in the completeness and sequence coverage of the built models has been observed.

Model quality assessment programs estimate the quality of protein models and can be used to estimate local error in protein models. ProQ3D is the most recent and most accurate version of our software. Here, it is demonstrated that it is possible to use local error estimates to substantially increase the quality of the models for molecular replacement (MR). Adjusting the B factors using ProQ3D improved the log-likelihood gain (LLG) score by over 50% on average, resulting in significantly more successful models in MR compared with not using error estimates. On a data set of 431 homology models to address difficult MR targets, models with error estimates from ProQ3D received an LLG of >50 for almost half of the models 209/431 (48.5%), compared with 175/431 (40.6%) for the previous version, ProQ2, and only 74/431 (17.2%) for models with no error estimates, clearly demonstrating the added value of using error estimates to enable MR for more targets. ProQ3D is available from http://proq3.bioinfo.se/ both as a server and as a standalone download.

A scanning soft X-ray spectromicroscope was recently developed based mainly on the photon-in/photon-out measurement scheme for the investigation of local electronic structures on the surfaces and interfaces of advanced materials under conditions ranging from low vacuum to helium atmosphere. The apparatus was installed at the soft X-ray beamline (BL17SU) at SPring-8. The characteristic features of the apparatus are described in detail. The feasibility of this spectromicroscope was demonstrated using soft X-ray undulator radiation. Here, based on these results, element-specific two-dimensional mapping and micro-XAFS (X-ray absorption fine structure) measurements are reported, as well as the observation of magnetic domain structures from using a reference sample of permalloy micro-dot patterns fabricated on a silicon substrate, with modest spatial resolution (e.g. ∼500 nm). Then, the X-ray radiation dose for Nafion® near the fluorine K-edge is discussed as a typical example of material that is not radiation hardened against a focused X-ray beam, for near future experiments.

An X-ray cross-correlation study of the impact of ligand composition and salt content on the self-assembly of soft-shell nanoparticles is presented, indicating symmetry-selective formation of order.

Today, Wednesday, May 25, astronomers unveiled the most complete 3-D map of the local universe (out to a distance of 380 million light-years) ever created. Taking more than 10 years to complete, the 2MASS Redshift Survey (2MRS) also is notable for extending closer to the Galactic plane than previous surveys – a region that’s generally obscured by dust.

The post Astronomers unveil the most complete 3-D map of the local universe appeared first on Smithsonian Insider.

A local supernova factory has recently started production, according to a wealth of new data from NASA's Chandra X-ray Observatory on the Carina Nebula.

The post The Carina Nebula, a local supernova factory, is ramping up production appeared first on Smithsonian Insider.

Last summer and this spring Boy Scout Arthur Carlton-Jones (shown here), working in conjunction with researchers at the Smithsonian Environmental Research Center in Edgewater, Md., […]

The post Save your local fishing hole appeared first on Smithsonian Insider.

Juan Martin D'Ambrosio, Veronique Albanese, Nicolas-Frederic Lipp, Lucile Fleuriot, Delphine Debayle, Guillaume Drin, and Alenka Copic

Osh6 and Osh7 are lipid transfer proteins (LTPs) that move phosphatidylserine (PS) from the endoplasmic reticulum (ER) to the plasma membrane (PM). High PS level at the PM is key for many cellular functions. Intriguingly, Osh6/7 localize to ER-PM contact sites, although they lack membrane-targeting motifs, in contrast to multidomain LTPs that both bridge membranes and convey lipids. We show that Osh6 localization to contact sites depends on its interaction with the cytosolic tail of the ER-PM tether Ist2, a homologue of TMEM16 proteins. We identify a motif in the Ist2 tail, conserved in yeasts, as the Osh6-binding region, and we map an Ist2-binding surface on Osh6. Mutations in the Ist2 tail phenocopy osh6 osh7 deletion: they decrease cellular PS levels, and block PS transport to the PM. Our study unveils an unexpected partnership between a TMEM16-like protein and a soluble LTP, which together mediate lipid transport at contact sites.

Sanjeev Chavan Nayak and Vegesna Radha

C3G (RapGEF1) plays a role in cell differentiation and is essential for early embryonic development in mice. In this study, we identify C3G as a centrosomal protein colocalizing with cenexin at the mother centriole in interphase cells. C3G interacts through its catalytic domain with cenexin, and they show interdependence for localization to the centrosome. C3G depletion caused a decrease in cellular cenexin levels. Centrosomal localization is lost as myocytes differentiate to form myotubes. Stable clone of cells depleted of C3G by CRISPR/Cas9 showed the presence of supernumerary centrioles. Overexpression of C3G, or a catalytically active deletion construct inhibited centrosome duplication. Cilia length is longer in C3G knockout cells, and the phenotype could be reverted upon reintroduction of C3G or its catalytic domain. Association of C3G with the basal body is dynamic, decreasing upon serum starvation, and increasing upon reentry into the cell cycle. C3G inhibits cilia formation and length dependent on its catalytic activity. We conclude that C3G inhibits centrosome duplication and maintains ciliary homeostasis, properties that may be important for its role in embryonic development.

An intuitive method is presented for detecting pseudosymmetries in Z' > 1 cases as a complement to well-proven strategies already available in the literature. It is based in the simple idea that the mid-points between equivalent atoms in symmetrically related mol­ecules are disposed according to simple well-known patterns, which are easily recognizable by optical inspection. A number of Z' = 4 cases in the literature are analyzed, which allows some of the potentialities of the method to be revealed.

Immigration benefits the U.S. economy overall and has little negative effect on the income and job opportunities of most native-born Americans, says a new report by a panel of the National Research Council.

Donations fill up the entryway to a Goodwill Southern California Donation Center in Pasadena during the first week of 2020.; Credit: Carla Javier/KPCC

Now that the holiday season is winding down, thrift shops run by Goodwill, the Salvation Army, and other organizations are tallying up the annual flood of December donations. 

"It's always been a tradition that our donors donate between Christmas and New Year's ... and the last couple days of the year, they donate even more," Goodwill Southern California director of logistics Tinna Bauer explained. "Some do it for tax purposes, and some ... when they if they receive new items for Christmas, they clean out the old."

This content is from Southern California Public Radio. View the original story at SCPR.org.

A new study has investigated the impacts of human activity on Antarctic Marine and Coastal. Alongside local effects of fishing, pollution and invasive alien species, global climate change is causing sea temperature rises and ocean acidification. Action is therefore needed at all levels to address these impacts.

A new tool has been developed, with input from the public, to measure and compare the quality of green spaces in urban areas. A UK case study suggests that litter and vandalism are among the factors most likely to prevent local residents from using and benefiting from green spaces.

A new German study suggests that residents??? level of satisfaction with their urban neighbourhood can be predicted from their perceptions of multiple and co-occurring burdens, such as poor air quality, lack of green space, noise and low cleanliness.

Density in the urban environment can encompass a multitude of factors such as population or dwelling density or the density of green areas. A new study surveyed professionals regarding how decisions on urban density are made, and has revealed that many feel that developers make most of these decisions, but that local authority planners should have more influence.

Local food systems, such as vegetable box schemes or farmers' markets, can encourage sustainable consumption. However, authorities must take care before becoming too involved in such citizen-led initiatives, because these collectives may be wary of government intervention, a new study suggests.

Some scientists have suggested that, when all potential inefficiencies are accounted for, local food distribution systems may be less sustainable than globalised systems. However, new research examining the behaviour of participants in local food networks in France suggests that they can be as energy efficient as globalised systems.

Messages that focus on the local impacts of climate change are among the most effective at reaching people who are generally dismissive of climate science, according a recent survey of Australian residents. The questionnaire asked participants about their attitudes and beliefs about climate change. Participants were shown a range of messages related to climate change adaptation, and then asked how much each message motivated them to take action. Presentations that contained local impacts, specific advice and negative emotive content were found to be the most effective.

Analysis of the operation of a novel, micro-scale anaerobic digester has shown that this technology could provide a useful means of processing food waste in urban areas. The study found that the digester, located in London and fed mainly with local food waste, avoided 3.9 tonnes of greenhouse gas emissions, while providing biogas for cooking, heat and power. Anaerobic digestion on this scale could play a part in reducing the amount of food waste that goes to landfill1 and contribute to the circular economy.

New research indicates that large-scale wind farms may influence local weather. It suggests the impact can be minimised by changing the design of the rotors or positioning wind farms in regions with high natural turbulence. These strategies could also make the farms more productive.

Construction of Concentrated Solar Power (CSP) plants – electricity generation which concentrates sunlight to generate heat – can have a range of negative effects on wildlife, but these effects are short lived, new research has found. Once in use, CSP plants can even have some positive effects, reducing soil erosion, for instance.

A new oil-spill risk-management system has been developed by researchers, which shows the likely effects of a coastal spill on the environment and economic activities for specific locations. It provides maps of oil-spill risk through a web portal and could help decision makers and emergency-response authorities protect the local environment and businesses through targeted and efficient planning and responses.

A new approach to modelling land use change developed at UBC could help cities and towns better coordinate their land-use planning efforts.

read more

The only way to establish a long-lasting and effective strategy for forest management is through collaboration between ‘conventional’ scientists and local experts, according to new research.

Locally managed carbon budgets for housing and transport could be highly effective in reducing greenhouse gas (GHG) emissions, according to researchers. They have suggested a policy framework with standardised methods to monitor city carbon budgets with local flexibility for remaining within these budgets.

Third party certification (TPC) uses independent auditors to ensure certain environmental standards are met. New research in Indonesia has indicated that TPC is not an objective process and is based on Western values and beliefs. It may need to incorporate different cultural practices and forms of knowledge to be globally effective.

New research from Vietnam has provided insight into factors that may influence the likelihood of local people supporting schemes that reward them for protecting services provided by the environment. It suggested that local scoping studies and open dialogue with participants are essential for these schemes to be successful.

A recent study has explored the role that renewable energy schemes can play in promoting sustainable development at the local level. It suggests that more must be done to encourage local investment and involvement in renewable energy projects if global sustainability objectives are to be met.

UN-REDD+ programmes that promote the sustainable use of forests may support local conservation and poverty alleviation goals, in addition to achieving global climate change benefits, according to a recent study. Existing multilateral funding schemes can provide important lessons for the development of UN-REDD+.

Despite the contribution that large-scale, intensive agriculture has made to global food production, food shortages still occur with particularly severe consequences for the poor. More investment in locally-led, small-scale farming would help ensure longer-term food security for the world’s most vulnerable under a changing climate and bring environmental benefits, according to a recent analysis of adaptation work in Uganda.

There has been a mixed response to Local Agenda 21 (LA21) across Europe, but a new study from the Basque Country in Spain has identified factors that contribute to a successful LA21 strategy. These are a local entrepreneurial presence, joint problem-solving and joint decision-making by local government members.

Researchers have proposed a new approach to identifying sustainability indicators for Integrated Coastal Zone Management (ICZM). It consists of three steps: identify the most important factors in ICZM for coastal stakeholders, model relationships between these factors, and analyse these factors to identify the most relevant for use as indicators.

Progress towards achieving a sustainable urban environment may be measured by sustainability indicators (SIs), which can be chosen to represent values that are important to local communities. A recent study has assessed a set of SIs developed by both sustainability experts and local citizens and suggests that local communities can attach different values to SIs to reflect local values and understandings of sustainability.

Agricultural land use presents a number of environmental challenges, which the European Commission is committed to addressing through a range of agri-environmental policies. A new study points to the importance of aligning agri-environmental policies with farmers’ needs and operations. Using the case of land clearing in Finland, the research underlines the importance of incorporating input from grassroots stakeholders into policy design.

For successful implementation of the Water Framework Directive, local planners will need specific targets, guidance on interpretation, and adequate resources to monitor progress, according to a recent study from Sweden.