Tony Ferguson hit out at lightweight rivals Conor McGregor and Khabib Nurmagomedov ahead of headlining UFC 249 against Justin Gaethje at Jacksonville, Florida, this weekend.

The 'en banc' D.C. Circuit Court will determine whether a congressional subpoena can be enforced by the courts.

Actress Rose McGowan called out Natalie Portman's Oscars cape that honored female directors, prompting Portman to defend her work made mostly with men.

STEPH MCGOVERN - who fronts her self-titled show The Steph Show on Channel 4 - made a cheeky admission on Twitter as her former BBC Breakfast co-star Bill Turnbull reacted to the presenter's "drunk" confession.

Floyd Mayweather has flirted with coming out of retirement for a third time.

McGraw, a three-star defensive end, announced his decision Sunday via Twitter.

McGraw, a three-star defensive end, announced his decision Sunday via Twitter.

McGraw, a three-star defensive end, announced his decision Sunday via Twitter.

Bokai Song
Apr 28, 2020; 0:RA120.001981v1-mcp.RA120.001981
Research

The cyclic GMP-AMP synthase (cGAS) protein is a pattern-recognition receptor of the mammalian innate immune system that is recognized as a main cytosolic sensor of pathogenic or damaged DNA. cGAS DNA binding initiates catalytic production of the second messenger, cyclic GMP-AMP, which activates the STING-TBK1-IRF3 signaling axis to induce cytokine expression. Post-translational modification (PTM) has started to be recognized as a critical component of cGAS regulation, yet the extent of these modifications remains unclear. Here, we report the identification and functional analysis of cGAS phosphorylations and acetylations in several cell types under basal and immune-stimulated conditions. cGAS was enriched by immunoaffinity purification from human primary fibroblasts prior to and after infection with herpes simplex virus type 1 (HSV-1), as well as from immune-stimulated STING-HEK293T cells. Six phosphorylations and eight acetylations were detected, of which eight PTMs were not previously documented. PTMs were validated by parallel reaction monitoring (PRM) mass spectrometry in fibroblasts, HEK293T cells, and THP-1 macrophage-like cells. Primary sequence and structural analysis of cGAS highlighted a subset of PTM sites with elevated surface accessibility and high evolutionary sequence conservation. To assess the functional relevance of each PTM, we generated a series of single-point cGAS mutations. Stable cell lines were constructed to express cGAS with amino acid substitutions that prevented phosphorylation (Ser-to-Ala) and acetylation (Lys-to-Arg) or that mimicked the modification state (Ser-to-Asp and Lys-to-Gln). cGAS-dependent apoptotic and immune signaling activities were then assessed for each mutation. Our results show that acetyl-mimic mutations at Lys384 and Lys414 inhibit the ability of cGAS to induce apoptosis. In contrast, the Lys198 acetyl-mimic mutation increased cGAS-dependent interferon signaling when compared to the unmodified charge-mimic. Moreover, targeted PRM quantification showed that Lys198 acetylation is decreased upon infections with two herpesviruses—HSV-1 and human cytomegalovirus (HCMV), highlighting this residue as a regulatory point during virus infection.

Juli Bai
Jun 1, 2019; 68:1099-1108
Perspectives in Diabetes

Racism makes our economy worse -- and not just in ways that harm people of color, says public policy expert Heather C. McGhee. From her research and travels across the US, McGhee shares startling insights into how racism fuels bad policymaking and drains our economic potential -- and offers a crucial rethink on what we can do to create a more prosperous nation for all. "Our fates are linked," she says. "It costs us so much to remain divided."

London : Macmillan, 1902.

Rockville, Maryland : National Institute on Drug Abuse, 1988.

Rockville, Maryland : National Institute on Drug Abuse, 1977.

Notre Dame coach Muffet McGraw retired after 33 seasons Wednesday. What she did for me in those four years, I came in as a girl and left as a woman.'' - WNBA player Kayla McBride, who played for Notre Dame from 2010-14.

Just two years removed from the euphoria of winning her second national championship, Muffet McGraw knew it was time. The Hall of Fame coach retired Wednesday with a resume that includes two national championships in 33 seasons at the school, a surprising decision to many of the countless players and coaches she has influenced on and off the court as a mentor and advocate for women. ''I am proud of what we have accomplished and I can turn the page to the next chapter in my life with no regrets, knowing that I gave it my best every day,'' said McGraw, a four-time winner of the AP women's basketball Coach of the Year.

Niele Ivey is coming home.

The two major companies cited what they considered onerous divestiture requirements from the U.S. Department of Justice as the reason behind their joint decision.

The post McGraw-Hill and Cengage Abandon Merger Plans appeared first on Market Brief.

Notre Dame women's basketball coach Muffet McGraw is retiring after winning two national championships in 33 years with the Irish.