velocityconf: RT @avleen: Ok folks, home stretch! We need 10 more people to record short #OpsSchool videos NEXT WEEK at @velocityconf. We can do it!...

The Amateur Traveler talks to again to Chris Willis (Swimming with Whales off Tonga - Episode 38, Ethiopia - Episode 79, Mountain Gorillas in Uganda and Rwanda - Episode 80, Travel to Mongolia - Episode 111) about his cruise with Quark Expeditions from Ushuaia, Argentina to Antarctica via the Falklands, South Georgia and South Orkney islands. We talk about wildlife photography of penguins, sea birds, whales and bad tempered fur seals.

The Amateur Traveler talks to Lee Abbamonte, the youngest American ever to travel to every country in the world, about 3 islands off the coast of Australia: Lord Howe Island, Norfolk Island, Christmas Island.

Hear about travel to the Silk Road in China's Gansu Province as the Amateur Traveler talks again to Lee Moore from Silk Road Hitchhikers about his trip to this historic and remote part of China.

Hear about hiking the Peaks of the Balkans trail in Albania, Kosovo, and Montenegro as the Amateur Traveler talks to Dan and Audrey from uncorneredmarket.com about this off the beaten track route in Europe.

Hear about walking the South Downs Way in England as the Amateur Traveler talks to Aaron Millar about this week-long trek.

Game designer and publisher Dave Gilbert founded Wadjet Eye Games in 2006.  This interview features conversation about point and click adventure games; digital game development, marketing and publishing; and the relationship between art, passion and real world commerce.

Jeffery Klaehn: How did you first become interested in point and click adventure games?

Dave Gilbert: I played King’s Quest at a very impressionable age! I typed the word “jump” and I saw Graham actually jump, and I was so blown away that I’ve been playing them ever since.

JK: You founded Wadjet Eye Games in 2006 to sell your game, The Shivah, commercially, then moved to pursue game design on a full-time basis and released The Blackwell Legacy, the first in what would become a series of five games.  What are your thoughts on these games and on the market then compared to now?

Dave Gilbert: I am Blackwell Legacy’s biggest critic. It was the first game I wrote with the intention of selling commercially – The Shivah was originally freeware, so I don’t count it – and it shows every inch of my inexperience. The gameplay is clunky, the story is told in three giant infodumps, and the main characters weren’t very likable. But that said, I know with absolute certainty that it was the very best game I could have made with the experience, resources and time I had available. So I stand by it.

As for the market, everything is different. Back in 2006, indie games in general were a very new thing. Read the rest

My pal Jake has been taking regular "cigar walks" (as he calls them) here in our lovely island city of Alameda, California. On those walks, he started spotting some creative flyers...

(I saved the best two for last...)

photos by Jake Schaffer, used with permission

Thanks, Susie! Read the rest

Video: Merlin Mann - "Time & Attention Talk (improvised)"

Audio (mp3): "Merlin Mann - 'Rutgers Time & Attention Talk'"

This is a talk I did at Rutgers earlier this month. I kinda like it, but for a weird reason. Something something, perfect storm of technology Ragnarok, and yadda yadda, I had to start the talk 20 minutes late with no slides. Nothing.

So, I riffed.

And, I ended up talking about a lot of the new stuff you can expect to see in the Inbox Zero book—work culture, managing expectations, the 3 deadly qualities of email, and one surprising reason email's not as much fun as Project Runway.

Some people liked it. I think. I liked it. I hope you do, too.

Here's the slides I would have shown. ;-)

Many thanks, again, to my great pal, Dr. Donald Schaffner, for bringing me in for this visit. I had a great time and met some fantastic, passionate people. Much appreciated.

update 2010-04-27_13-50-00

Apologies—my friends at Rutgers (inexplicably) have placed this video under lock and key. Fortunately, I have a lock-picker called Firefox. Samizdat video available soon...

update 2010-04-27_14-42-24

Yay, fixed! Many thanks to my hero, Jesse Schibilia.

The following article, MI Barber Shop Owner Defies Gov. Whitmer Shutdown Order…Opens Business…Police Officer Walks In…Says, “I love you!”…Walks Out, was first published on 100PercentFedUp.com.

Last week, we reported about how Shelley Luther, owner of the Salon Á La Mode in North Dallas, Texas, who opened up her business in defiance of lockdown orders in the city. The salon owner said that she was ignoring a citation and a cease and desist order from the city to shut down. This one […]

Continue reading: MI Barber Shop Owner Defies Gov. Whitmer Shutdown Order…Opens Business…Police Officer Walks In…Says, “I love you!”…Walks Out ...

The following article, Donald Trump Jr. Offers To Walk Bride-to-Be Down The Aisle After Liberal Parents Refuse To Attend Her Wedding Because Fiance Is A Conservative, was first published on 100PercentFedUp.com.

TDS or Trump Derangement Syndrome brings out the worst in a lot of people. One young Texan girl’s story of her intolerant parents who refuse to attend her wedding because she’s marrying a conservative, however, is a new low. Lawyer and conservative civil rights activist, Rogan O’Handley tweeted a Tik Tok video of a Texan […]

Continue reading: Donald Trump Jr. Offers To Walk Bride-to-Be Down The Aisle After Liberal Parents Refuse To Attend Her Wedding Because Fiance Is A Conservative ...

The following article, 77-Yr-Old MI Barber Defies Gov Whitmer’s Shutdown Order: ”I don’t need another mother” Shop Will Stay Open Until “Jesus walks in or until they arrest me” [VIDEO], was first published on 100PercentFedUp.com.

Emergency orders that “non-essential businesses” remain closed during the Covid-19 crisis have devastated small business owners across America. A wave of business owners across the nation are defying executive orders by their governors and opening their businesses anyhow. Yesterday, we reported about a small business owner in the state of Michigan, where Governor Gretchen Whitmer […]

Continue reading: 77-Yr-Old MI Barber Defies Gov Whitmer’s Shutdown Order: ”I don’t need another mother” Shop Will Stay Open Until “Jesus walks in or until they arrest me” [VIDEO] ...

Numerous zinc ectoenzymes are metalated by zinc and activated in the compartments of the early secretory pathway before reaching their destination. Zn transporter (ZNT) proteins located in these compartments are essential for ectoenzyme activation. We have previously reported that ZNT proteins, specifically ZNT5–ZNT6 heterodimers and ZNT7 homodimers, play critical roles in the activation of zinc ectoenzymes, such as alkaline phosphatases (ALPs), by mobilizing cytosolic zinc into these compartments. However, this process remains incompletely understood. Here, using genetically-engineered chicken DT40 cells, we first determined that Zrt/Irt-like protein (ZIP) transporters that are localized to the compartments of the early secretory pathway play only a minor role in the ALP activation process. These transporters included ZIP7, ZIP9, and ZIP13, performing pivotal functions in maintaining cellular homeostasis by effluxing zinc out of the compartments. Next, using purified ALP proteins, we showed that zinc metalation on ALP produced in DT40 cells lacking ZNT5–ZNT6 heterodimers and ZNT7 homodimers is impaired. Finally, by genetically disrupting both ZNT5 and ZNT7 in human HAP1 cells, we directly demonstrated that the tissue-nonspecific ALP-activating functions of both ZNT complexes are conserved in human cells. Furthermore, using mutant HAP1 cells, we uncovered a previously-unrecognized and unique spatial regulation of ZNT5–ZNT6 heterodimer formation, wherein ZNT5 recruits ZNT6 to the Golgi apparatus to form the heterodimeric complex. These findings fill in major gaps in our understanding of the molecular mechanisms underlying zinc ectoenzyme activation in the compartments of the early secretory pathway.

7 May 2020 , Volume 96, Number 3

7 May 2020 , Volume 96, Number 3

Expert

N. N. Leonenko, I. Papić, A. Sikorskii and N. Šuvak
Theor. Probability and Math. Statist. 99 (2020), 137-147.
Abstract, references and article information

Let’s discuss student opinion forms, course evaluations, student evaluation of teaching forms, whatever term you use. Article after article year after year highlight how toxic they are. More recently the emphasis has been on how they differ according to instructor … Continue reading →

Numerous zinc ectoenzymes are metalated by zinc and activated in the compartments of the early secretory pathway before reaching their destination. Zn transporter (ZNT) proteins located in these compartments are essential for ectoenzyme activation. We have previously reported that ZNT proteins, specifically ZNT5–ZNT6 heterodimers and ZNT7 homodimers, play critical roles in the activation of zinc ectoenzymes, such as alkaline phosphatases (ALPs), by mobilizing cytosolic zinc into these compartments. However, this process remains incompletely understood. Here, using genetically-engineered chicken DT40 cells, we first determined that Zrt/Irt-like protein (ZIP) transporters that are localized to the compartments of the early secretory pathway play only a minor role in the ALP activation process. These transporters included ZIP7, ZIP9, and ZIP13, performing pivotal functions in maintaining cellular homeostasis by effluxing zinc out of the compartments. Next, using purified ALP proteins, we showed that zinc metalation on ALP produced in DT40 cells lacking ZNT5–ZNT6 heterodimers and ZNT7 homodimers is impaired. Finally, by genetically disrupting both ZNT5 and ZNT7 in human HAP1 cells, we directly demonstrated that the tissue-nonspecific ALP-activating functions of both ZNT complexes are conserved in human cells. Furthermore, using mutant HAP1 cells, we uncovered a previously-unrecognized and unique spatial regulation of ZNT5–ZNT6 heterodimer formation, wherein ZNT5 recruits ZNT6 to the Golgi apparatus to form the heterodimeric complex. These findings fill in major gaps in our understanding of the molecular mechanisms underlying zinc ectoenzyme activation in the compartments of the early secretory pathway.

(Yale University) The 2020 Gruber Cosmology Prize recognizes Lars Hernquist, Center for Astrophysics | Harvard & Smithsonian, and Volker Springel, Max Planck Institute for Astrophysics, for their defining contributions to cosmological simulations, a method that tests existing theories of, and inspires new investigations into, the formation of structures at every scale from stars to galaxies to the universe itself.

(University of Johannesburg) Fly ash generated by coal-fired power stations is a global environmental headache, creating groundwater and air pollution from vast landfills and ash dams. The waste product can be repurposed into geopolymer concrete, such as precast heat-cured structural elements for buildings. However, a critical durability problem has been low resistance to extreme alkali attack. UJ researchers found that high temperature heat-treatment at 200 degrees Celsius can halve this harmful mechanism in fly ash geopolymer concretes.

Chief Justice Patience Roggensack faced backlash for her comment, with some people calling it "elitist" to separate meatpackers from "regular folks."

Gary Lichtenstein Editions has partnered with Urban Pathways to increase awareness and safety in the homeless community.

The development of the HUPO-PSI's (Proteomics Standards Initiative) standard data formats and MIAPE (Minimum Information About a Proteomics Experiment) guidelines should improve proteomics data sharing within the scientific community. Proteomics journals have encouraged the use of these standards and guidelines to improve the quality of experimental reporting and ease the evaluation and publication of manuscripts. However, there is an evident lack of bioinformatics tools specifically designed to create and edit standard file formats and reports, or embed them within proteomics workflows. In this article, we describe a new web-based software suite (The ProteoRed MIAPE web toolkit) that performs several complementary roles related to proteomic data standards. Firstly, it can verify the reports fulfill the minimum information requirements of the corresponding MIAPE modules, highlighting inconsistencies or missing information. Secondly, the toolkit can convert several XML-based data standards directly into human readable MIAPE reports stored within the ProteoRed MIAPE repository. Finally, it can also perform the reverse operation, allowing users to export from MIAPE reports into XML files for computational processing, data sharing or public database submission. The toolkit is thus the first application capable of automatically linking the PSI's MIAPE modules with the corresponding XML data exchange standards, enabling bidirectional conversions. This toolkit is freely available at http://www.proteored.org/MIAPE/.

7 May 2020 , Volume 96, Number 3

If a bulk-loading process fails when you use SAS with SAS/ACCESS Interface to Oracle, you will receive the warning: "WARNING: All or some rows were rejected/discarded.: The actual error is "SQL*Loader-2026: The load was aborted because SQL

When you bulk load data into a Google BigQuery database, you might encounter this error: "Error while reading data, error message: CSV table encountered too many errors, giving up...Error detected while parsing row starting at position: 0...Data

As Matt Duffy entered the offseason, he knew that he needed to add strength in order to hold up over the course of a 162-game season. The 28-year old has been at the Rays' Spring Training facility in Port Charlotte, Fla., for about a week, and he believes his improved shape will help the team fight for a playoff spot in 2019.

Numerous zinc ectoenzymes are metalated by zinc and activated in the compartments of the early secretory pathway before reaching their destination. Zn transporter (ZNT) proteins located in these compartments are essential for ectoenzyme activation. We have previously reported that ZNT proteins, specifically ZNT5–ZNT6 heterodimers and ZNT7 homodimers, play critical roles in the activation of zinc ectoenzymes, such as alkaline phosphatases (ALPs), by mobilizing cytosolic zinc into these compartments. However, this process remains incompletely understood. Here, using genetically-engineered chicken DT40 cells, we first determined that Zrt/Irt-like protein (ZIP) transporters that are localized to the compartments of the early secretory pathway play only a minor role in the ALP activation process. These transporters included ZIP7, ZIP9, and ZIP13, performing pivotal functions in maintaining cellular homeostasis by effluxing zinc out of the compartments. Next, using purified ALP proteins, we showed that zinc metalation on ALP produced in DT40 cells lacking ZNT5–ZNT6 heterodimers and ZNT7 homodimers is impaired. Finally, by genetically disrupting both ZNT5 and ZNT7 in human HAP1 cells, we directly demonstrated that the tissue-nonspecific ALP-activating functions of both ZNT complexes are conserved in human cells. Furthermore, using mutant HAP1 cells, we uncovered a previously-unrecognized and unique spatial regulation of ZNT5–ZNT6 heterodimer formation, wherein ZNT5 recruits ZNT6 to the Golgi apparatus to form the heterodimeric complex. These findings fill in major gaps in our understanding of the molecular mechanisms underlying zinc ectoenzyme activation in the compartments of the early secretory pathway.

4 February 2020

We could dance on Gravity, We could burst the Sun, We could be that horror Alien who stole your child, We are born metal touching galaxies though glass, Galileo’s kids seeing through Sombrero, We could look after water, Or it’s facsimile, We could look after Earth.                                                                                                                                                         evocative short poetry

She shifts with the breeze, Neon white with blue streaks, Antenna filtering the air for blossoms, Fabulous, Owning the street, Owning the couple, At sunset before, The African roundabout, A butterfly that will not let go, Wafting beyond reach, Before the hawk and Gently anyway – Ever been a glass-wearer looking for your glasses with, […]

It is raining outside. I am reminded that, Rain has rhythm. Rhythm has heart-beat, A beat, a Rat-a-tat-tat, Every city has its gangsters, its’ Street corners, its Unbelievers, every Heart has its beat, and Every beating heart, its’ God, Where folly is a pink bear, An African American, A bionic car round the bend, Sunlight […]

THE NOMENCLATURE ‘roots reggae artiste’ takes on a whole new classification when referencing singer Winston McAnuff. An elder statesman who has a fixation with an instrument which retains its genesis in the Church – the accordion – McAnuff, also...

1. The political aspirant is about to find out just how expensive it is to get a gritty, old veteran to give up his parliamentary seat. Our sources say many millions are being demanded. So, once again, they are all looking to shake the money tree...

Steve Wilkerson enters Orioles camp competing for a super-utility role after a brief big league cameo last year. He isn't traveling light, as Wilkerson has 10 gloves on display.

The Three Talk Model of shared decision is a framework to help clinicians to think about how to structure their consultation to ensure that shared decision making can most usefully take place. The model is based around 3 concepts - option talk, decision talk, and team talk - with active listening at the centre. Three Talk was first proposed in...

On the podcast, we’ve talked a lot about the limits of medicine - where treatment doesn’t work, or potentially harms. But in that conversation, we’ve mainly focused on specific treatments. Now a new analysis, broadens that to talk about patients being admitted to a whole ward - intensive care. The authors of that article contend that, often,...