"I think it's always important to think about suicide as individual vulnerabilities and context," Eric Caine said.

Pakistanis crowded markets on Saturday after a nationwide coronavirus lockdown was eased, despite the country declaring its second highest daily infection toll. Prime Minister Imran Khan has allowed businesses to reopen in phases from the weekend, citing the economic havoc the virus restrictions have wreaked on the improvised nation. In the garrison city of Rawalpindi, thousands of shoppers were preparing for Eid, which follows the Muslim fasting month of Ramadan, with many flouting social distancing rules and advice to wear masks.

Protease-activated receptor 2 (PAR-2) is activated by secreted proteases from immune cells or fungi. PAR-2 is normally expressed basolaterally in differentiated nasal ciliated cells. We hypothesized that epithelial remodeling during diseases characterized by cilial loss and squamous metaplasia may alter PAR-2 polarization. Here, using a fluorescent arrestin assay, we confirmed that the common fungal airway pathogen Aspergillus fumigatus activates heterologously-expressed PAR-2. Endogenous PAR-2 activation in submerged airway RPMI 2650 or NCI–H520 squamous cells increased intracellular calcium levels and granulocyte macrophage–colony-stimulating factor, tumor necrosis factor α, and interleukin (IL)-6 secretion. RPMI 2650 cells cultured at an air–liquid interface (ALI) responded to apically or basolaterally applied PAR-2 agonists. However, well-differentiated primary nasal epithelial ALIs responded only to basolateral PAR-2 stimulation, indicated by calcium elevation, increased cilia beat frequency, and increased fluid and cytokine secretion. We exposed primary cells to disease-related modifiers that alter epithelial morphology, including IL-13, cigarette smoke condensate, and retinoic acid deficiency, at concentrations and times that altered epithelial morphology without causing breakdown of the epithelial barrier to model early disease states. These altered primary cultures responded to both apical and basolateral PAR-2 stimulation. Imaging nasal polyps and control middle turbinate explants, we found that nasal polyps, but not turbinates, exhibit apical calcium responses to PAR-2 stimulation. However, isolated ciliated cells from both polyps and turbinates maintained basolateral PAR-2 polarization, suggesting that the calcium responses originated from nonciliated cells. Altered PAR-2 polarization in disease-remodeled epithelia may enhance apical responses and increase sensitivity to inhaled proteases.

Contact between inflammatory cells and endothelial cells (ECs) is a crucial step in vascular inflammation. Recently, we demonstrated that the cell-surface level of endomucin (EMCN), a heavily O-glycosylated single-transmembrane sialomucin, interferes with the interactions between inflammatory cells and ECs. We have also shown that, in response to an inflammatory stimulus, EMCN is cleared from the cell surface by an unknown mechanism. In this study, using adenovirus-mediated overexpression of a tagged EMCN in human umbilical vein ECs, we found that treatment with tumor necrosis factor α (TNF-α) or the strong oxidant pervanadate leads to loss of cell-surface EMCN and increases the levels of the C-terminal fragment of EMCN 3- to 4-fold. Furthermore, treatment with the broad-spectrum matrix metalloproteinase inhibitor batimastat (BB94) or inhibition of ADAM metallopeptidase domain 10 (ADAM10) and ADAM17 with two small-molecule inhibitors, GW280264X and GI254023X, or with siRNA significantly reduced basal and TNFα-induced cell-surface EMCN cleavage. Release of the C-terminal fragment of EMCN by TNF-α treatment was blocked by chemical inhibition of ADAM10 alone or in combination with ADAM17. These results indicate that cell-surface EMCN undergoes constitutive cleavage and that TNF-α treatment dramatically increases this cleavage, which is mediated predominantly by ADAM10 and ADAM17. As endothelial cell-surface EMCN attenuates leukocyte–EC interactions during inflammation, we propose that EMCN is a potential therapeutic target to manage vascular inflammation.

Letter submitted by Marc R. Rosenblum, Deputy Director, U.S. Immigration Program, at the request of the Minority Staff of the House Oversight and Government Reform Committee for the February 25, 2015 joint subcommittee hearing reviewing apprehension, detention, and release policies regarding unauthorized immigrants.

The Nova Scotia government is going ahead with its annual lottery for moose hunting licences after the latest survey found Cape Breton moose numbers have rebounded from a low of 1,300 last year.

A Texas A&M AgriLife Research team has good news for patients with copper-deficiency disorders, especially young children diagnosed with Menkes disease.

In the ongoing struggle to address health care disparities in rural communities across the U.S., a recent Oregon State University study found that the perfect partner may be hiding in plain sight.

An OMer sharing the gospel at a police station in Turkey is asked to return and carry on the conversation.

Officials in Italy say an Italian aid worker who was kidnapped in Kenya in late 2018 has been freed in Somalia

Images show man lunging toward an officer who had tried to calm him down.

"Life is incomplete without that special someone, who lights up every moment of life," wrote Salman Khan

"Life is incomplete without that special someone, who lights up every moment of life," wrote Salman Khan

Both, the BMW F 900 XR and the S 1000 XR are expected to be launched by mid this year with bookings likely to open next month.

The Ducati Panigale V2 draws power from a 955cc, V-twin Superquadro engine which is now BS6/Euro5 compliant and pumps out 151 horses.

The Honda Dio has got a minor price for both its variants.

On MCX, gold June futures rose Rs 37 to Rs 45,788 per 10 grams. Similarly, silver July futures were trading Rs 403 or 0.96 per cent higher at Rs 42,299 per kg

With theatres being shut since for over a month now, many small and mid-rung production houses are understood to be in talks with video over-the-top (OTT) platforms for direct-to-digital launch of their unreleased movies.

Over 200 companies in Pune’s industrial belt of Chakan, Talegaon, Kurkumb, Baramati and Ranjangaon have resumed operations after restrictions were eased on Monday, even as the lockdown continues.

Retail experts estimate that the current situation might wipe off 10% of e-commerce companies’ annual GMV in the country.

NII is the difference between interest earned and interest paid by a bank. The lender’s asset quality improved q-o-q with gross non-performing assets (NPA) of 17.36%, down 10 basis points over Q2FY19.

In India, which is the biggest democracy in the world with over 1.3 billion people, the question of public health has never been debated in public.

Three children, including a five-year-old boy, have died of a mysterious inflammatory syndrome resembling Kawasaki disease or a toxic shock, Governor Andrew Cuomo said, as the state authorities began an inquiry into the cases.
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The government had last month extended the deadline to file GST returns for March to May 5, from April 20. As per convention, the government releases GST revenue collection number on the basis of cash collection in a particular month.

As most of the small and medium scale industries have started operations from 20th April, the normal consumption level of petrol and diesel is likely to be achieved by July 2020.

The government has decided to borrow Rs 12 lakh cr in the current fiscal, compared to Rs 7.8 lakh cr earlier. 

Number of tourists visiting Hong Kong has been increased with around 2,54,000 visitors in the country with an increase of 9%.According to the Immigration Department, the current golden week holiday has witnessed strong development in the number of tourists…

The district magistrates will explore the possibility of sending those Tablighi members, who belong to other states, in buses to their designated places in accordance with social distancing norms and other protocols, DDMA Special CEO K S Meena said in a letter to deputy commissioners (administration).

It was good news for renewable energy when President Barack Obama in June proposed carbon dioxide restrictions on existing power plants. It is even better news now that he may use the plan to leverage an international climate accord.