The Amateur Traveler talks to Italophile Ira Bernstein who returns to the show to talk about day trips in Tuscany that you can take from Florence. We talk about visiting the walled city of Siena and its famous bareback horse race the palio, viewing the many towers of San Gimignano, climbing the leaning tower of Pisa (after you take the obligatory picture of someone holding it up), and walking on the walls of Lucca. Each town has its own character and attractions. Arezzo has a jewelry museum and the home of the “Father of Humanism” Petrarch. Fiesole is a rich enclave, a suburb of Florence with views of the city and a roman theatre. Montepulciano and Montalcino are both known for their wines. Ira talks about some of the events that the locals enjoy like the palios, flea markets and flag throwing competitions. So even if you just get as far out of Florence as Piazzale Michelangelo there are many places to explore in Tuscany.

The Amateur Traveler talks to Kara from Miami about her hometown of Miami. Kara tells us Miami is not as it is pictured in TV shows like Miami Vice and Burn Notice (except perhaps that it is filled with beautiful people). She tells us about some of her favorite hotels and restaurants as well as cafes where you can sit and people watch. Miami is known for its beach life and Kara tells us where the daring can go to bare all or where you can find a quiet spot away from the tourists. Increasingly Miami is known for its fashion district and its food scene. The summers are hot but offer some of the best bargains on restaurants, hotels and spas… if you can deal with the humidity. Miami defines hot in all senses of the word. So whether you want to see snowbirds or eat stone crabs, Miami might be a place you should check out.

News

Muslim hostess and Disney still at odds over headscarf
French Flight Attendant Arrested for Stealing Cash and Jewels From Sleeping Passengers’ Pockets and Carry-Ons

Show Notes

Diamond PR
Miami Vice
Burn Notice
Haulover Beach
South Pointe Park
Top Ten Miami Restaurants
Brickell, Miami
Key Biscayne
Miami Museums
Miami Design District
Van Dyke Cafe
Miami Spice Restaurant Month
Miami Spa Month
Neighborhoods of Miami
Miami Design District
Michael’s Genuine Food and Drink
Calle Ocho Festival
South Beach Food and Wine Festival
Miami Public Transportation
Fontainebleau
Miami Heat
Miami Heat Tickets
Smith and Wollensky
Red, the Steakhouse
Joe’s Stone Crabs
Adrianne Arsht Center
Snowbirds

Community

Scott enjoyed the group interview in Travel to Namibia – Episode 243
Tricia hits the road
Amateur Traveler guest post guidelines
Amateur Traveler site redesign to add countries

The Amateur Traveler talks to Chuck Prevatte about vacationing in the warm waters and on the white beaches of the panhandle of Florida. This is a vacation for those who want to slow down and relax. There are nightclubs in Panama City and water parks but this part of Florida is a good spot to take the family, eat some good sea food, rent a beach house and bath in 90 degree water. Tourism is off because of the gulf oil spill but Chuck and his family did not see much evidence of the oil on the beaches.

The Amateur Traveler talks to Alan from Jacksonville about St. Augustine Florida which is the oldest continually inhabited European settlement in what is now the United States. St. Augustine was founded in 1565 and is 42 years older than Jamestown.

The Amateur Traveler talks to Shannon Martin from Two Who Travel about her hometown of Tampa Bay (and St Petersburg) Florida. Shannon recommends the Tamp Bay area for a beach vacation, possible combined with a trip to Walt Disney World in Orlando.

Hear about travel to Orlando, Florida as the Amateur Traveler talks to Jason Cochran who is the editor of Frommers.com and the editor to the Frommer's Guide to Orlando Florida about vacationing in America's favorite destination spot. We talk about Walt Disney World, the increasingly popular Universal Studios, with a mention of some of the other things to do in Orlando.

Hear about travel to South Florida (Miami, Everglades National Park, and the Florida Keys) as the Amateur Traveler talks to Erik Smith from onmyfeetorinmymind.com about this diverse region.

Everybody wanted to get to the shore last weekend when the state of Florida reopened its beaches. But one of the beachgoers who came in for some extra attention was a six-foot-long bull shark caught near Navarre Beach, according to WKRG-TV. Video shot by Shelley Goudy of Fort Walton showed several men gathered around the…

The post Florida Fishermen Nab 6-Foot Bull Shark During First Weekend of Reopened Beaches appeared first on The Western Journal.

MPU 046: Workflows with Merlin Mann II « Mac Power Users

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I have the life that I have because I've made a lot of weird decisions, and they've worked out well.

Not gonna lie to you. I'm a huge nerd. Surprised?

Yep. I can recite big chunks of The Big Lebowski from memory. I can argue for an hour on the merits of Dick York over Dick Sargent. And, I can—and frequently do—catch myself thinking Catwoman, Batgirl, Princess Leia, and Emma Peel should have a light-hearted pillow fight that ends with an hour of genial french-kissing.

Pretty much like you, probably. I dunno, maybe your version includes Kitty Pryde. Po-tay-to/Po-taht-o, right?

Perhaps most saliently, by virtue of having spent a solid 2,399 days as a Fake Productivity Guru, I have been provided with an unquestionably Janusian monkey's paw of a gift; I now know a lot about workflows. Nerdy, nerdy workflows.

I can tell you a few things that almost always work, I can tell you a handful of things that almost never work, and—best or worst of all—I can tell you thousands of things that might work. Sometimes. Maybe. Kinda. For some people. For now.

And, at the risk of gay-marrying my arrogance to my hypocrisy, I can tell you that I also know enough about the unholy diarrhea of potential options for Theoretical Productivity to share two big patterns:

1. Getting your workflow right matters.
2. Getting your workflow right to the exclusion of the actual work is a fool's game.

But. Managing to get the most useful and most elegant and least fiddly mix of 1 and 2 right is super-hard. Especially for nerds. Especially for me.

So, as I type this today, I believe there can be no greater testament to these claims—or, at least, no greater place to test the veracity of these claims for yourself—than in this TWO AND A HALF HOUR-long interview for Mac Power Users.

It is reeeeeeeeally nerdy. Almost intolerably nerdy. Just…overwhelmingly nerdy.

But, man, is it ever really good, and really fat with the most insanely granular details of How I Work.

Lo, even these 928.5 days after officially retiring from productivity pr0n, my desire to not "vend stroke material for your joyless addiction to puns about procrastination and systems for generating more taxonomically satisfying meta-work" is tempered by a (widely under-reported) practical streak.

Yes: I continue to despise empty advice about rearranging deck chairs on The Titanic. But, yes: I do also still very much enjoy talking about how all the tips and tricks can or can't work in the context of work you care about. That matters. It really does.

So. Here goes. A one hundred and forty six minute-long, Joyce-ian amble through the Big Stuff and the Little Stuff. David and Katie were very patient.

How I name text files. Why I break iOS apps. Why I love the letter "x." Why I won't row out to islands any more. How a 115,000 word book manuscript is "like a house full of confederate money." How "The Cloud" broke in New Zealand. How I use MultiMarkdown, Scrivener, TextExpander, OmniFocus, TextMate, Notational Velocity, Dropbox, and an explosive combination of Elements, Notesy, Nebulous, Simplenote, CF Outliner, iThoughts, Instacast, Good Reader, and wow wow wow.

How I try not to fiddle—how I sometimes succeed and often don't. But, how I try.

Anyhow. There you go. A perfectly nerdy bookend to last year's first Magnum Opus MPU interview on these same topics, Mac Power Users Episode 46 is just insanely nerdy. And, what have you.

I hope you like it. I hope it's useful. I hope you don't use it to replace real work.

And, as ever, I really hope Batgirl starts having more sexy pillow fights.

Enjoy. And, God save you.

MPU 046: Workflows with Merlin Mann II « Mac Power Users

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Prince Harry visited Chatham House on 15 June to open the Stavros Niarchos Foundation Floor.

1 August 2014 , Volume 70, Number 4

The map (click below to download the PDF) shows how the proportion of migrants living in countries of the European Union has changed since 1990.

• The Perks of Being A Wallflower Review
• Beasts of the Southern Wild Review
• What We Watched

Sea ice is one of the least understood components of our climate. Naturally its abundance or scarcity is a telling sign of climate change, but sea ice is also an important actor in change as well, insulating the ocean and reflecting sunlight. A branch of mathematics called percolation theory helps explain how salt water travels through sea ice, a process that is crucial both to the amount of sea ice present and to the microscopic communities that sustain polar ecosystems. By taking samples, doing on-site experiments, and then incorporating the data into models of porous materials, mathematicians are working to understand sea ice and help refine climate predictions. Using probability, numerical analysis, and partial differential equations, researchers have recently shown that the permeability of sea ice is similar to that of some sedimentary rocks in the earth.s crust, even though the substances are otherwise dissimilar. One major difference between the two is the drastic changes in permeability of sea ice, from total blockage to clear passage, that occur over a range of just a few degrees. This difference can have a major effect on measurements by satellite, which provide information on the extent and thickness of sea ice. Results about sea ice will not only make satellite measurements more reliable, but they can also be applied to descriptions of lung and bone porosity, and to understanding ice on other planets. Image: Pancake ice in Antarctica, courtesy of Ken Golden. For More Information: "Thermal evolution of permeability and microstructure in sea ice," K. M. Golden, et al., Geophysical Research Letters, August 28, 2007.

Sea ice is one of the least understood components of our climate. Naturally its abundance or scarcity is a telling sign of climate change, but sea ice is also an important actor in change as well, insulating the ocean and reflecting sunlight. A branch of mathematics called percolation theory helps explain how salt water travels through sea ice, a process that is crucial both to the amount of sea ice present and to the microscopic communities that sustain polar ecosystems. By taking samples, doing on-site experiments, and then incorporating the data into models of porous materials, mathematicians are working to understand sea ice and help refine climate predictions. Using probability, numerical analysis, and partial differential equations, researchers have recently shown that the permeability of sea ice is similar to that of some sedimentary rocks in the earth.s crust, even though the substances are otherwise dissimilar. One major difference between the two is the drastic changes in permeability of sea ice, from total blockage to clear passage, that occur over a range of just a few degrees. This difference can have a major effect on measurements by satellite, which provide information on the extent and thickness of sea ice. Results about sea ice will not only make satellite measurements more reliable, but they can also be applied to descriptions of lung and bone porosity, and to understanding ice on other planets. Image: Pancake ice in Antarctica, courtesy of Ken Golden. For More Information: "Thermal evolution of permeability and microstructure in sea ice," K. M. Golden, et al., Geophysical Research Letters, August 28, 2007.

Sea ice is one of the least understood components of our climate. Naturally its abundance or scarcity is a telling sign of climate change, but sea ice is also an important actor in change as well, insulating the ocean and reflecting sunlight. A branch of mathematics called percolation theory helps explain how salt water travels through sea ice, a process that is crucial both to the amount of sea ice present and to the microscopic communities that sustain polar ecosystems. By taking samples, doing on-site experiments, and then incorporating the data into models of porous materials, mathematicians are working to understand sea ice and help refine climate predictions. Using probability, numerical analysis, and partial differential equations, researchers have recently shown that the permeability of sea ice is similar to that of some sedimentary rocks in the earth.s crust, even though the substances are otherwise dissimilar. One major difference between the two is the drastic changes in permeability of sea ice, from total blockage to clear passage, that occur over a range of just a few degrees. This difference can have a major effect on measurements by satellite, which provide information on the extent and thickness of sea ice. Results about sea ice will not only make satellite measurements more reliable, but they can also be applied to descriptions of lung and bone porosity, and to understanding ice on other planets. Image: Pancake ice in Antarctica, courtesy of Ken Golden. For More Information: "Thermal evolution of permeability and microstructure in sea ice," K. M. Golden, et al., Geophysical Research Letters, August 28, 2007.

Sea ice is one of the least understood components of our climate. Naturally its abundance or scarcity is a telling sign of climate change, but sea ice is also an important actor in change as well, insulating the ocean and reflecting sunlight. A branch of mathematics called percolation theory helps explain how salt water travels through sea ice, a process that is crucial both to the amount of sea ice present and to the microscopic communities that sustain polar ecosystems. By taking samples, doing on-site experiments, and then incorporating the data into models of porous materials, mathematicians are working to understand sea ice and help refine climate predictions. Using probability, numerical analysis, and partial differential equations, researchers have recently shown that the permeability of sea ice is similar to that of some sedimentary rocks in the earth.s crust, even though the substances are otherwise dissimilar. One major difference between the two is the drastic changes in permeability of sea ice, from total blockage to clear passage, that occur over a range of just a few degrees. This difference can have a major effect on measurements by satellite, which provide information on the extent and thickness of sea ice. Results about sea ice will not only make satellite measurements more reliable, but they can also be applied to descriptions of lung and bone porosity, and to understanding ice on other planets. Image: Pancake ice in Antarctica, courtesy of Ken Golden. For More Information: "Thermal evolution of permeability and microstructure in sea ice," K. M. Golden, et al., Geophysical Research Letters, August 28, 2007.

Albert Chau and Man-Chun Lee
Trans. Amer. Math. Soc. 373 (2020), 3627-3647.
Abstract, references and article information

N. Kuznetsov
St. Petersburg Math. J. 31 (2020), 521-531.
Abstract, references and article information

The banking sector today launched a scheme to alleviate the cashflow pressure faced by corporate customers in light of the economic challenges brought about by the COVID-19 outbreak.

Jointly announced by the Monetary Authority and the Banking Sector SME Lending Coordination Mechanism, the Pre-approved Principal Payment Holiday Scheme is estimated to cover more than 80% of all corporate borrowers in Hong Kong.

All corporate customers that have an annual sales turnover of $800 million or less and with no outstanding loan payments overdue for more than 30 days are eligible for the scheme.

Eligible customers’ loan principal payments due within a six-month period between May 1 and October 31 this year will be pre-approved for deferment.

The loans’ principal payments, including revolving facilities, will generally be deferred by six months, whereas trade facilities, given their short-term nature, will be deferred by three months.

The scheme does not cover syndicated loans or loans used for financing purchases of shares or other financial assets.

Banks will begin informing eligible customers of the pre-approval under the scheme as soon as practicable. Eligible customers do not need to apply. They only need to contact the bank within 14 days of the bank’s notice to confirm the detailed arrangements.

The authority has issued a circular requesting all banks to participate in the scheme and has received support from all 11 major lenders in the co-ordination mechanism.

It will monitor the scheme’s operation and maintain close dialogue with banks.

This SAS Note provides instructions about how to enable SAS Workflow Studio log generation and debugging in order to troubleshoot a problem in SAS Workflow Studio. On the client machine where&

(Florida Museum of Natural History) Florida and Georgia archaeologists have discovered the location of Fort San Antón de Carlos, home of one of the first Jesuit missions in North America. The Spanish fort was built in 1566 in the capital of the Calusa, the most powerful Native American tribe in the region, on present-day Mound Key in the center of Estero Bay on Florida's Gulf Coast.

(Florida Museum of Natural History) More than a thousand years ago, people from across the Southeast regularly traveled to a small island on Florida's Gulf Coast to bond over oysters, likely as a means of coping with climate change and social upheaval.

(American Association for the Advancement of Science) Microplastic particles entering the sea surface were thought to settle to the seafloor directly below them, but now, a new study reveals that slow-moving currents near the bottom of the ocean direct the flow of plastics, creating microplastic hotpots in sediments of the deep sea.

(University of Manchester) An international research project has revealed the highest levels of microplastic ever recorded on the seafloor, with up to 1.9 million pieces in a thin layer covering just 1 square meter.

(Marine Biological Laboratory) Microbial life is bubbling up to the ocean floor along with fluids from deeply buried petroleum reservoirs, reports a team of scientists from the University of Calgary and the Marine Biological Laboratory, Woods Hole.

(University of Hawaii at Manoa) In a study published in Scientific Reports, researchers at the University of Hawai'i at Mānoa, found in the next few decades, sea level rise will likely cause large and increasing percentages of land area to be impacted simultaneously by the three flood mechanisms. Further, they found direct marine inundation represents the least extensive--only three percent of the predicted flooding, while groundwater inundation represents the most extensive flood source.

(Clemson University) While the world admires the beauty and fragrance of flowers, most of us are missing out on the extraordinary processes these seemingly delicate life forms are carrying out every moment of the day. Matthew Koski, an assistant professor at Clemson, is not only paying attention, he is advancing his research with a three-year, $455,000 grant from the NSF for a study of flower adaptations titled 'Modifying the floral microenvironment: elevational divergence in floral thermoregulatory mechanisms.'

Despite being placed under strict restrictions and COVID-19 curfew measures, shoppers, commuters, motorists, and vendors in Montego Bay got a feel of Christmas on Wednesday, when they were showered with items as part of the COVID-19 giveaway by an...

David Cluet
Apr 1, 2020; 19:701-715
Technological Innovation and Resources

Eelke P. Béguin
Apr 24, 2020; 0:RA120.001964v1-mcp.RA120.001964
Research

We present a technological advancement for the estimation of the affinities of Protein-Protein Interactions (PPIs) in living cells. A novel set of vectors is introduced that enables a quantitative yeast two-hybrid system based on fluorescent fusion proteins. The vectors allow simultaneous quantification of the reaction partners (Bait and Prey) and the reporter at the single-cell level by flow cytometry. We validate the applicability of this system on a small but diverse set of PPIs (eleven protein families from six organisms) with different affinities; the dissociation constants range from 117 pm to 17 μm. After only two hours of reaction, expression of the reporter can be detected even for the weakest PPI. Through a simple gating analysis, it is possible to select only cells with identical expression levels of the reaction partners. As a result of this standardization of expression levels, the mean reporter levels directly reflect the affinities of the studied PPIs. With a set of PPIs with known affinities, it is straightforward to construct an affinity ladder that permits rapid classification of PPIs with thus far unknown affinities. Conventional software can be used for this analysis. To permit automated analysis, we provide a graphical user interface for the Python-based FlowCytometryTools package.

Games! Tech Advisor's Lewis Painter and Dom Preston school Henry Burrell and Techword's Scott Carey on the haps from E3. What does E3 stand for? What is a rabbid? Which games have eagles in them? All the issues. Scott then talks us through Uber's latest mishaps, which by now are not funny - will the company survive the mess its CEO has led it into?  

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This manuscript has been withdrawn by the Author.

The vessel wall is continuously exposed to hemodynamic forces generated by blood flow. Endothelial mechanosensors perceive and translate mechanical signals via cellular signaling pathways into biological processes that control endothelial development, phenotype and function. To assess the hemodynamic effects on the endothelium on a system-wide level, we applied a quantitative mass spectrometry approach combined with cell surface chemical footprinting. SILAC-labeled endothelial cells were subjected to flow-induced shear stress for 0, 24 or 48 hours, followed by chemical labeling of surface proteins using a non-membrane permeable biotin label, and analysis of the whole proteome and the cell surface proteome by LC-MS/MS analysis. These studies revealed that of the >5000 quantified proteins 104 were altered, which were highly enriched for extracellular matrix proteins and proteins involved in cell-matrix adhesion. Cell surface proteomics indicated that LAMA4 was proteolytically processed upon flow-exposure, which corresponded to the decreased LAMA4 mass observed on immunoblot. Immunofluorescence microscopy studies highlighted that the endothelial basement membrane was drastically remodeled upon flow exposure. We observed a network-like pattern of LAMA4 and LAMA5, which corresponded to the localization of laminin-adhesion molecules ITGA6 and ITGB4. Furthermore, the adaptation to flow-exposure did not affect the inflammatory response to tumor necrosis factor α, indicating that inflammation and flow trigger fundamentally distinct endothelial signaling pathways with limited reciprocity and synergy. Taken together, this study uncovers the blood flow-induced remodeling of the basement membrane and stresses the importance of the subendothelial basement membrane in vascular homeostasis.

When the problem occurs, you are unable to perform any workflow actions in a SAS solution that uses SAS Workflow Services.

The problem occurs on a content release on the SAS Risk Governance Framework.

The problem occurs on a content release on the SAS Risk Governance Framework.

You might see the following error in SAS Workflow Studio when you try to open the CECL_Cycle_AFS workflow template that is shipped with SAS Solution for CECL:

Sodium glucose co-transporter-2 inhibitors (SGLT2i) have favorable cardiovascular outcomes in diabetic patients. However, whether SGLT2i can improve obesity-related cardiac dysfunction is unknown. Sestrin2 is a novel stress-inducible protein that regulates AMPK-mTOR and suppresses oxidative damage. The aim of this study was to determine whether empagliflozin (EMPA) improves obesity-related cardiac dysfunction via regulating Sestrin2-mediated pathways in diet-induced obesity. C57BL/6J mice and Sestrin2 knockout mice were fed a high-fat diet (HFD) for 12 weeks and then treated with or without EMPA (10 mg/kg) for 8 weeks. Treating HFD-fed C57BL/6J mice with EMPA reduced body weight, whole-body fat, and improved metabolic disorders. Furthermore, EMPA improved myocardial hypertrophy/fibrosis and cardiac function, and reduced cardiac fat accumulation and mitochondria injury. Additionally, EMPA significantly augmented Sestrin2 levels, increased AMPK and eNOS phosphorylation, but inhibited Akt and mTOR phosphorylation. These beneficial effects were partially attenuated in HFD-fed Sestrin2 knockout mice. Intriguingly, EMPA treatment enhanced the Nrf2/HO-1-mediated oxidative stress response, suggesting antioxidant and anti-inflammatory activity. Thus, EMPA improved obesity-related cardiac dysfunction via regulating Sestrin2-mediated AMPK-mTOR signaling and maintaining redox homeostasis. These findings provide a novel mechanism for the cardiovascular protection of SGLT2i in obesity.

The pancreatic islet is a highly-vascularized endocrine micro-organ. The unique architecture of rodent islets, a so-called core-mantle arrangement seen in 2D images, led researchers to seek functional implications for islet hormone secretion. Three models of islet blood flow were previously proposed, all based on the assumption that islet microcirculation occurs in an enclosed structure. Recent electrophysiological and molecular biological studies using isolated islets also presumed uni-directional flow. Using intravital analysis of the islet microcirculation in mice, we find that islet capillaries are continuously integrated to those in the exocrine pancreas, which makes the islet circulation rather open, not self-contained. Similarly in human islets, the capillary structure was integrated with pancreatic microvasculature in its entirety. Thus, islet microcirculation has no relation to islet cytoarchitecture, which explains its well-known variability throughout species. Furthermore, tracking fluorescent-labeled red blood cells at the endocrine-exocrine interface revealed bi-directional blood flow, with similar variability in blood flow speed in both the intra- and extra-islet vasculature. To date, the endocrine and exocrine pancreas have been studied separately by different fields of investigators. We propose that the open circulation model physically links both endocrine and exocrine parts of the pancreas as a single organ through the integrated vascular network.