La Luciérnaga se enciende para hablar de la llegada de Colombia a la final del mundial femenino de fútbol. Además, ¿Que pasa con la gestión de los congresistas? También, le contamos todo lo que debe saber sobre la entrada en funcionamiento de Hidroituango.La Luciérnaga, un espacio de humor, análisis y opinión de Caracol Radio que acompaña desde hace 30 años a sus oyentes en el regreso a casa.

La Luciérnaga se enciende para hablar de la creación del ministerio de la igualdad. Además, le contamos sobre qué está pasando con Hidroituango. Tambien, ¿Qué pasa con la salud en Colombia?La Luciérnaga, un espacio de humor, análisis y opinión de Caracol Radio que acompaña desde hace 30 años a sus oyentes en el regreso a casa.

La Luciérnaga se enciende para hablar del descenso en las cifras de desempleo en el país. Además, le contamos sobre qué está pasando con Hidroituango. Tambien, ¿Qué pasa con el fútbol colombiano?La Luciérnaga, un espacio de humor, análisis y opinión de Caracol Radio que acompaña desde hace 30 años a sus oyentes en el regreso a casa.

La Luciérnaga se enciende para hablar de la reforma política. Además, le contamos sobre el anuncio del presidente Petro de nombrar a jovenes como gestores de paz. También, siguel el mundial de Qatar 2022.La Luciérnaga, un espacio de humor, análisis y opinión de Caracol Radio que acompaña desde hace 30 años a sus oyentes en el regreso a casa.

En 6AM Hoy por Hoy de Caracol Radio estuvo John Maya Salazar, gerente general de EPM, para hablar sobre qué esperan del cierre del túnel de desviación derecho de Hidroituango.

De meeste smartwatches die tot nu toe gelanceerd zijn waren vooral een accessoire voor je smartphone. Ze werkten via bluetooth als tweede scherm. De TrueSmart van Omate is echter een standalone smartwatch die als volwaardig apparaat gebruikt kan worden en nog waterdicht is ook.

Great name, better music.

Fans of all things under the sea now have a new way of exploring the mysteries and wonders of Bermuda’s unique marine life as the Bermuda Zoological Society’s Bermuda Reef Life HD app is now available free for Android users. A spokesperson said, “The app, which was first launched for Apple iOS devices in November […]

Google анонсировала обновление для приложений с камерой, которое порадует всех любителей фотографии. Теперь поддержка формата RAW будет доступна не только в продвинутых приложениях, но и в более простых камерах на Android.

Согласно новым утечкам, Google готовится вернуть популярную функцию настройки иконок, которая была доступна в Android 11.

Jupiter has often been thought to protect the inner Solar System from asteroids and comets, but new research has shown that the giant planet may actually increase the risk of an impact.

Detonating a nuclear weapon on or near an asteroid is one of several options for defending the Earth from an impact. Here's what nuking an asteroid might actually do, and why it isn't always the best option.

When a skyscraper-sized asteroid narrowly misses Earth in 2029, three spacecraft may be along for the ride.

A Planetary Society-funded project to understand asteroids achieved its main goals and scientific objectives this year.

The European Space Agency’s Hera spacecraft launched on Oct. 7, 2024, from Cape Canaveral, Florida. It will travel to the Didymos-Dimorphos asteroid system to study the aftermath of the first-ever field test of an asteroid deflection technique.

Potentially dangerous comets could be spotted many years in advance by following the meteoroid trails they leave near Earth, new research shows.

The apparent non‑gravitational acceleration the extra-solar-system ‘Oumuamua exhibits is puzzling. We find that when the position and velocity of the Sun is correctly set in computing the predicted orbit, ‘Oumuamua’s trajectory can be explained with gravity and we have reproduced the unexpected gap by computation. We also propose to search for new extra-solar-system high speed...

Today, we’re going to finish up the embroidery on the sample leaf for Harvest Hues. If you’re just joining in, …

Good morning, and Happy Monday! We’ll jump right into the exciting news – the embroidery kits for The Christmas Mitten …

Cybersecurity researchers have discovered a new version of a well-known Android malware family dubbed FakeCall that employs voice phishing (aka vishing) techniques to trick users into parting with their personal information. "FakeCall is an extremely sophisticated Vishing attack that leverages malware to take almost complete control of the mobile device, including the interception of incoming

Google has warned that a security flaw impacting its Android operating system has come under active exploitation in the wild. The vulnerability, tracked as CVE-2024-43093, has been described as a privilege escalation flaw in the Android Framework component that could result in unauthorized access to "Android/data," "Android/obb," and "Android/sandbox" directories, and their respective

Over 1,500 Android devices have been infected by a new strain of Android banking malware called ToxicPanda that allows threat actors to conduct fraudulent banking transactions. "ToxicPanda's main goal is to initiate money transfers from compromised devices via account takeover (ATO) using a well-known technique called on-device fraud (ODF)," Cleafy researchers Michele Roviello, Alessandro Strino

Cyber threats are intensifying, and cybersecurity has become critical to business operations. As security budgets grow, CEOs and boardrooms are demanding concrete evidence that cybersecurity initiatives deliver value beyond regulation compliance. Just like you wouldn’t buy a car without knowing it was first put through a crash test, security systems must also be validated to confirm their value.

Oh dear, busy me, four months since I've posted here. Lots of quilting, a little beading, some travels, and recently several sets of house guests have made "retirement" a bit of a joke. For now, since the La Conner Quilt Festival is just around the corner (October 2-4), my subject is one small exhibition on the first floor at the Museum, which is currently up, and will remain through the Festival.

Last year at the Festival (and again this year!) Sue Spargo taught workshops on her wool applique embroidery methods. Those of us who were lucky enough to get in were invited by the Museum to exhibit our finished pieces. These marvelous creations are the subject of this post. Uneven lighting makes it difficult to get good photos, so please forgive poor color or tone on some of these photos.

In case you are unfamiliar with Sue's work, below is an example of her work. Students in her class could choose to make chicks, circles, or flowers. Most chose the chicks. Eight of her students are showing their work in the current exhibit.

My vote for the most awesome-creative piece goes to Bunny Starbuck for She Has Flown the Coop! Here it is:

Having put my chicks all in neat rows similar to Sue's example, I find it amazing that Bunny's mind took the idea and made a whole different story of it... a coop full of chicks, with two in line on the roof ready to follow the one already in the air. Don't you just love the way the lines divide the space?!




Note Bunny's use of whispy yarn to suggest little chick feathers, her use of snaps for eyes, the one chick turned full front, the one with a bead-tassled braid and glasses, and the one with beaded loop fringe and a gathered yellow ribbon (?) yo-yo. I love them! I'm in awe, Bunny!

Below is Chicks on Parade by Dorie Benson. It's so fun the way they are so close together, with two of them going in a different direction, just as you might see in a little cluster of chicks. If you click the photo to enlarge it, you can see some of Dorie's excellent stitching and hand quilting!

The next one is Chicks by Glenys Baker, who not only did all 36 chicks but also made a second quilt featuring circles (shown further down). Awesome work, Glenys!

And next is Birds of a Feather Can Dance Together by Lorraine Jones. The fun of the title is matched by the fun of Lorraine's embellishments, including her border treatment. (You'll have to see this one in person, because the light from a table lamp, made the lower corner of the photo look terrible.)

I guess most of us like to "swim the other way," as you can see in the next piece, Chickadees, by Carrie Unick. I thought many of Corrie's embellishments were especially fun and creative, so I've also included a couple of detail photos.



Last of the chicks is my piece, simply named Chicks. It was so much fun, that I made another small piece (also in the exhibition), shown at the top of this post. Both are displayed in shadow-box type frames to protect them from dust and moth damage.
Glenys Baker, in one year, made not only a 36-chicks quilt (shown above), but also a 48-circles quilt, Circles. How she keeps thinking of new embellishment variations I can't imagine. Yet each of her circles is completely unique! Her choice of border fabrics is perfect for the quilt, complimenting the flow of color among the circles.


I love the fun color combinations and the way the beads enhance her embroidery on Nancy Anders' Bodacious Blooms, a joyful wall quilt if ever there was one! (Again, I couldn't quite get the color right in the photo, so you'll just have to go to the Museum to see it in person.)


The name of Roberta Roberts' flower quilt, Memories, invites us to look closely at the flower with three ladybugs. I don't know the story, but I can tell there is one here... and I love that. Her embellishments are varied and fun as well.



There you have it... 10 quilts by 8 students who learned some methods of wool applique and embroidery from Sue Spargo, last year at the La Conner Quilt Festival. I'm so grateful to be one of them!

The quilting community is awesomely generous!!!  Thanks to all who have sent me scraps for making hexie flowers:


I really understand and appreciate that it takes time to sort through your scraps, selecting some that you feel might work for me, packing them to send, getting them to the post office. In some cases, you even took time to cut them into 2" squares for me... WOW! Bless your hearts!

The most interesting thing is that all the fabrics I've received so far are ones I might have picked myself, yet so far there are no duplicates. As of Oct. 19th, I have cut petal sets for about 650 flowers (from my own scraps and fat quarter stash, as well as scraps I've received from other quilters), all of them unique! That's about 90% of what I need to make the quilt, and certainly enough to keep me busy for a looooooong time. It will probably take me a year or so to baste and stitch all of the flowers, although I admit to going at it with a lot of gusto.

During the past 28 years of stitching beads on cloth, I've veered away from making necklaces, bracelets, and bags or purses, mostly producing wall art pieces. Thus, I've had lots of opportunities to experiment with various methods of finishing and framing my work, making it suitable for display.


I always leave ample fabric margins surrounding the actual beaded area, giving me lots of options for finishing or framing.  One simple finishing method is to wrap the extra fabric around the sides of a manufactured painting-canvas (over stretcher-bars), and lace the fabric snugly across the back, as in the photo below.


But the problem with this method, of course, is that the surface of the beading is exposed to dust and air-borne grime, which over the years may spoil the fresh look of the beading, aging the piece prematurely. So we look to frames and glass to protect our work.

Among the various methods for covering (and protecting) beadwork with glass, the following is the one I use most frequently, and like the best.  It's not very expensive, or particularly difficult, although it does take a bit of time. Even if you already know about frames, please take a look at the section called Preparing Your Bead Embroidery for Framing in the Tutorial below.

Tutorial: How to Frame bead Embroidery

Choosing a Frame

As the first-ever “full-scale planetary defense test” to deflect a space rock, the DART mission aims to show that protecting Earth from a hazardous asteroid is possible.

The debris NASA’s asteroid-touching spacecraft collected could help us learn about the origins of our solar system. But for that to happen, scientists have to protect it from just about everything.

Two hours before asteroid 2022 EB5 entered Earth’s atmosphere, scientists knew exactly when and where the space rock would strike.

The mission is a test to see if NASA could knock an Earth-bound asteroid off its path, should we ever need to.

In this tutorial, I'll show you how to install the ALIUNDE Kodi Addon on your Android TV box or Amazon Firestick, plus, unique features in ALIUNDE.

In this guide, we have put together the best 5 Android smart TVs with Android TV pre-installed. You can install Kodi right onto these TVs easily. These TVs are available from Amazon and can be purchased worldwide.

In this tutorial guide, I'll show you exactly how to install the M-E-T-V Kodi Addon on your Android TV box or Amazon Firestick, and unique features.

In this tutorial, I'll show you exactly how to install the SEALTEAM 6 Kodi Addon on your Android TV box or Amazon Firestick, plus unique features.

Chikankari specialty Step into a world where every thread tells a story and every pattern sings a story song. Chikankari House stands not only as an advertisement but as the custodian of a centuries-old art form. Traditional Indian embroidery is not only preserved here; It is celebrated, nurtured and evolved. Chikankari with its subtle beauty and intricate details weaves the fabric of India’s rich cultural story. Chikankari House: Carrying forward a valuable asset Chikankari-property History and Origins Chikankari House takes great pride in being the torchbearer of an art form that has deep roots in ancient India. Known for its exquisite beauty and subtlety, Chikankari is often associated with the Nawab’s land in Lucknow. This delicate weaving style is believed to have flourished during the Mughal era under the patronage of Noor Jahan, wife of Emperor Jehangir. Chinkari, however, is more than just embroidery and decoration; It symbolizes an interesting story told through fashion. The story begins in mythology, where Lord Krishna’s wife Radha is said to have expressed her love with unique colorful chikankari patterns embroidered on cloth This Connection imbues chikankari with divine substance and emotional meaning, and owes it and rich textiles of rich cultural heritage again Lucknow is situated. Evolution Over the Years Like a river that changes its course over time yet never abandons its source, Chikankari too went through various transformations over the centuries. Yet, it painstakingly preserved its original essence. Chikankari artisans of the past passed on their art to younger generations, ensuring that it continued with all its skill and fervour. Notable figures in history have contributed to the popularity of this intricate craft over the years. British viceroys, Queen Victoria herself, and more recently, celebrities, and fashion designers have all played crucial roles in keeping Chikankari in the public consciousness. In its nascent stages, Chikankari was typically portrayed in white-on-white embroidery on muslin cloth, epitomizing the essence of understated elegance. Over time, the craft embraced colored threads, sequins, beads, and various fabric types, transforming Chikankari into a versatile and appealing element in modern fashion. Through the eye of the House of Chikankari, this beautiful amalgamation of past and present turns a simple piece of fabric into an alluring canvas. And as the world evolves, so do we, making sure the essence of Chikankari, the legacy of Kesari, continues to enthral generations to come. The Craftsmanship of Chikankari: An Intricate Symphony of Stitches Understanding the Art When it comes to understanding the art of Chikankari, the process begins even before the first stitch is laid. The House of Chikankari starts its crafting journey with the selection of just the right fabric – one that holds onto the charm of the delicate stitches, absorbing the soul of Lucknow into its texture. Traditionally, fabrics like muslin, cotton, and silk have been used owing to their soft texture and durability. The art features multiple unique stitches, each lending a distinctive texture to the piece. One such stitch, traditionally known as the “Bakhia” or the shadow work, stands as the backbone of Chikankari. Symbolic of Chikankari’s subtlety, Bakhia is an intricate technique where the thread’s shadow reflects on the cloth, creating a play of light and shade that brings the fabric to life. The artist behind the craft Behind every piece of Chikankari art from Chikankari House is an artist – patient, dedicated and full of unimaginable skill. They are quiet poets who tell stories through their work, weaving stories of tradition and heritage. Becoming a Chikankari artisan is not a job but a professional journey, often passed down through generations. Their training usually begins at an early age, and they spend years mastering the details – from learning different fabrics to understanding patterns and knots While it’s hard to put a number on the many unsung heroes nurturing this art form, an estimated 2.5 million artists across India are associated with Chikankari, according to the European Center for Development Policy and Management , sources that this art show succeeds on a global stage, spreading the appeal of our cultural heritage far and wide. House of Chikankari Today The House of Chikankari, rooted deeply in traditional artistry, is embracing the winds of change to sail towards modernity with grace and resilience. Today, this time-honored craft marries age-old techniques with contemporary influences, gracefully striding into the global fashion arena. Modern Innovations In the realm of Chikankari, modern technology and evolving fashion sensibilities play pivotal roles in elevating this craft to cater to contemporary tastes without losing its essence. Digital Design and Promotion: Techniques such as digital embroidery design and online marketing are helping Chikankari reach global audiences. The House of Chikankari leverages social media and e-commerce to showcase its intricate designs worldwide. New Materials and Stitches: While preserving the traditional stitches, innovative materials like georgette and net are being used, along with traditional cotton and silk. The inclusion of embellishments such as sequins and beads adds to the allure of modern Chikankari outfits. Fashion Integration: Notable fashion designers and brands, including the House of Chikankari, have been instrumental in incorporating Chikankari into high fashion. Collections showcased at major fashion weeks have featured Chikankari, placing it on the global fashion map. Sustaining the Craft The journey of Chikankari, from the streets of Lucknow to the fashion capitals of the world, has been supported by various efforts to preserve its legacy. Government and NGO Efforts: Initiatives like the Geographical Indications (GI) tag for Lucknow Chikankari by the Indian government have aided in protecting its identity. NGOs and social enterprises collaborate with artisans, providing them with fair wages and better work conditions. Educational Programs: Specialized training programs and workshops aim at skill enhancement and introducing the youth to Chikankari, ensuring the inheritance of this craft by future generations. Exhibitions and Workshops: Events such as the “Chikankari Exhibition” in major cities and the participation of Chikankari craft in international craft fairs increase awareness and appreciation for this delicate embroidery work. Through these efforts, the House of Chikankari not only survives but thrives in the landscape of modern fashion and global markets. The blend of traditional craftsmanship with contemporary innovation ensures that Chikankari remains not just a craft of the past but a living tradition, evolving and growing with each stitch in time. Investing in Chikankari Investing in Chikankari is not just a purchase—it’s an embrace of a heritage, a story woven into fabric. This section provides a comprehensive guide to ensure you select authentic pieces and maintain them, so they last a lifetime. Guide to Buying Chikankari Selecting Authentic Pieces Look for Handcraftsmanship: Authentic Chikankari is hand-embroidered. Look closely at the stitching. Irregularities often indicate handwork, as opposed to the uniform stitches of machine embroidery. Fabric Quality: Traditional Chikankari is done on natural fabrics like cotton, silk, and muslin. A gentle touch and the slightly irregular texture of natural fabrics are good indicators of quality. Design Details: Authentic Chikankari flaunts intricate designs that can range from floral motifs to paisley and geometric patterns. The richness in details often signifies authentic workmanship. Where to Buy: Prefer purchasing from recognized artisans, local markets in Lucknow, or reputable online stores specializing in Chikankari. Some reputable brands connected to the House of Chikankari offer quality assurance. Price Range and Factors Affecting Cost Artisan vs. Mass-Produced: Handcrafted Chikankari pieces, especially those from skilled artisans, will be priced higher than mass-produced ones. The uniqueness and quality justify the cost. Complexity of Work: The more intricate the embroidery and the denser the design, the higher the price. Work that takes longer to produce is naturally more valuable. Fabric Type: The choice of fabric impacts the price. Silk-based Chikankari garments are pricier than those crafted on cotton due to the cost of the material. Caring for Chikankari Garments To ensure your Chikankari pieces remain as timeless as their tradition, following proper care instructions is crucial. Washing Instructions: It’s best to hand wash Chikankari clothes in cold water with a mild detergent. Avoid wringing the fabric harshly. For machine wash, use a gentle cycle. Drying and Ironing: Always dry Chikankari garments in the shade. Direct sunlight can fade the delicate threads. While ironing, place a thin cloth over the garment to prevent direct contact with the iron. Storage: Store Chikankari apparel in a cool, dry place. Use padded hangers for items like sarees and kurtas to avoid creases. For best preservation, wrap the garments in soft, breathable fabric. By following these guidelines, you can invest wisely in Chikankari, ensuring you own a piece of heritage that brings elegance and traditional charm to your wardrobe. This enduring craftsmanship, with the right care, can be part of your legacy, cherished and passed down through generations. Chikankari Future As we navigate the crowded world of fashion and fashion, the House of Chikankari remains open in the face of obstacles and opportunities. Navigating a sea of ​​change, the delicate beauty of embroidery clings to its heritage. Challenges and opportunities Challenges Competition with machine-made products: One of the most important issues is competition from faster and cheaper looms that imitate Chikankari. Preservation of traditional skills: There is a danger that the meticulous skills of Chikankari craftsmen will be lost in today’s fast-paced manufacturing environment. Economic Development: Designers often face financial challenges due to irregular demand for materials and a lot of time spent on crafts. opportunity Design innovation: Innovation that captures the essence of Chikankari while appealing to contemporary aesthetics can create new appeal. Online Marketplace: With the rise of e-commerce, Chikankari offers a global platform, connecting skilled artisans with consumers directly. Sustainable clothing: As consumers become more eco-conscious, demand for sustainable and ethically produced clothing such as chikankari may increase. What is happening in the future Trends in the fashion industry, as well as consumer preferences, point to a different future for Chikankari. There is the possibility that: Fusion Wear: Combine chikankari and western fashion elements for unique outfits. Heritage promotion: The use of chikankari as a contemporary fashion can be a cultural promotion, especially among the youth. Technology integration: Technology can provide immersive shopping experiences such as virtual reality, allowing consumers to interact with artistic origins and stories. conclusion Chikankari House, an Indian heritage fortress plays an important role in traditional handloom weaving. It’s not just historical art preservation; It supports the livelihood of countless artists. Cultural Identity: Supporting Chikankari is like preserving an irreplaceable part of India’s cultural identity. Investing in Heritage: Each purchase of a chikankari is an investment in a tradition that has been handed down for generations. Call to action: Explore the timeless beauty of authentic chikankari embroidery. There is a story embedded in every thread. Sharing history of every purchase. Chikankari House is not just alive; it is adaptive, conquering, and moving forward to meet dog after dog adorned with canine charms. It invites us to be not only spectators but participants in the preservation of this complex art form.

The post House of Chikankari: Unveiling the Secrets Behind Exquisite Embroidery appeared first on Geeky Traveller.

Wigan Athletic goalkeeper Tom Watson saves all three penalties in a shootout victory over Nottingham Forest U21s to send them through to the EFL Trophy knockout phase.

1. NASA Alert: Massive 'God of Chaos' Asteroid Apophis Makes Closest Pass by Earth Yet  Bizz Buzz
2. NASA alert! 'God of Chaos' asteroid approaching Earth on November 13 sparks worldwide concern  The Times of India
3. Nasa Alert: Massive 'God of Chaos' asteroid Apophis nears earth in closest pass yet  CNBCTV18
4. Giant Meteorite To Hit Earth On November 13? What We Know  News18
5. 'Astonishingly close': A colossal space rock could wreak havoc on Earth in 2029  Business Today

One of the greatest pass rushers in NFL history appears to be taking a pass himself when it comes to directly addressing a raging controversy. Pro Football Hall of Famer […]

The post NFL Analyst Michael Strahan Speaks Out After Becoming Embroiled in National Anthem Controversy appeared first on The Western Journal.

dannyhennesy posted a photo:

On the Capital planet the rebellious droids had followed maily the Bat-Bot, but as time progressed his circuits had gone all mushy at 780 years or so without maintenance…

Several splinter groups all with their local bot leaders emerged such as the Che-bot, the traffic-light-robot and the Butt-bot, but none of these collected enough sentient circuits to call themselves a popular (or Animata) mass movement!

That was until a cyborg came along, one known as Jones, a long time prisoner and terrorist, his easy solutions to every problem rang well in the masses' auditory circuits!!!

His slogans and simple rhetoric were simple enough for the simple traffic-light to comprehend and cheer!

His language was full of hate towards the organics and especially the humans who were the most common races among the ruling class of the federation!!!

Despite being a “Fleshie” himself his message collected the angry enslaved
bot community by only weeks all rebellious robots except for a few fringe loonies had forgotten the old leaders…

One morning at Jones gave the signal…

All over the capital planet hordes and swarms of any form of mechanical sentient beings attacked first the police stations, then the Company boards running the planet and the federation as well as their starfleet…

Many died, especially the low level police and army! Many mechanicals died too, but their ranks were soon filled by Mutant fleshie allies of the lower levels who hated the Federation feudal society and upper classes as much as their technological allies…

The Federation state apparatus and ruling class, most of their fleet army fled when they knew the game was up, they activated the emergency escape plan and whole city blocks with important factories, administrational units, valuable assets and so on separated from the capital by hidden rocket engines and homed in their course to Mars…

On Mars the federation regrouped and formed their new society…

On the Capital planet, the robots proclaimed the first Techno-republic of the advanced inorganic civilization, the low level fleshies left behind, became slaves and their mutant allies got to rule their own minute chiefdoms as protectorates under the Techno-republic…

Jones was now the undisputed ruler of the capital planet, but the victory was a pyrros one since, all important buildings, all of value was now one Mars!

But as Jones put it:

Our proud race the Techno-species didn’t need the Fleshies administration, their infrastructure, their spaceships…

We shall start from scratch, with a new administration, a new order, every droid shall work at 4x speed than they did during human oppression since now we are free and the fleshies shall work twice as hard than the Techno-Race, until we have breed enough new fleshies so they can do all work!

Our future is bright and shiny like glistering shiny metal!

The snapshot seen here is from the first police station attacked in sector 45-34v-ss-g the first one to fall according to official techno-history!

———————————————/
Designers note:

I am sad to say that this is the last episode in this years-spanning space series… At least for a while, I will still post LEGO hobby stuff here but without a storyline, perhaps small designs and builds… and occasionally a story when I feel like it!!!

I would like to thank all who had been in this journey of our heros, but it has taken far to much time and effort and since the state of the world is as it is, I am spiraling down in another depression, I must stop it before I reach the abyss, so I have remove some stress out of my equation… I ended it in a cliffhanger so I can easily restart it when my mental health improves… I hope that won’t be forever???

I would love if someone used my characters or ideas, please send me a link if you do, I would love to read it or look at it!!!

But there will be more Lego, just in different format without long stories, I need to focus more on my art and to be honest that is the only time the mental pain eases, when I create!!!


Peace and Noise!

MushroomBrain a FOL

Pectins are a major dietary nutrient source for the human gut microbiota. The prominent gut microbe Bacteroides thetaiotaomicron was recently shown to encode the founding member (BT1017) of a new family of pectin methylesterases essential for the metabolism of the complex pectin rhamnogalacturonan-II (RG-II). However, biochemical and structural knowledge of this family is lacking. Here, we showed that BT1017 is critical for the metabolism of an RG-II–derived oligosaccharide ΔBT1017oligoB generated by a BT1017 deletion mutant (ΔBT1017) during growth on carbohydrate extract from apple juice. Structural analyses of ΔBT1017oligoB using a combination of enzymatic, mass spectrometric, and NMR approaches revealed that it is a bimethylated nonaoligosaccharide (GlcA-β1,4-(2-O-Me-Xyl-α1,3)-Fuc-α1,4-(GalA-β1,3)-Rha-α1,3-Api-β1,2-(Araf-α1,3)-(GalA-α1,4)-GalA) containing components of the RG-II backbone and its side chains. We showed that the catalytic module of BT1017 adopts an α/β-hydrolase fold, consisting of a central twisted 10-stranded β-sheet sandwiched by several α-helices. This constitutes a new fold for pectin methylesterases, which are predominantly right-handed β-helical proteins. Bioinformatic analyses revealed that the family is dominated by sequences from prominent genera of the human gut microbiota, including Bacteroides and Prevotella. Our re-sults not only highlight the critical role played by this family of enzymes in pectin metabolism but also provide new insights into the molecular basis of the adaptation of B. thetaiotaomicron to the human gut.

Visual Abstract

Madison T Wright
Nov 18, 2020; 0:RA120.002168v1-mcp.RA120.002168
Research

En fonction de leurs causes, les douleurs du côté droit peuvent se manifester de différentes manières. Les symptômes de ces douleurs peuvent également varier d’une personne à une autre. Certaines personnes décrivent les douleurs du côté comme des sensations de crampes, de brûlures ou de piqûres. D’autres en revanche parlent de douleurs sourdes ou lancinantes qui irradient […]

L’article Douleurs côté droit sous les côtes : comment les soulager naturellement est apparu en premier sur Ortho Doc France.

Thyroglobulin (Tg) is a secreted iodoglycoprotein serving as the precursor for T3 and T4 hormones. Many characterized Tg gene mutations produce secretion-defective variants resulting in congenital hypothyroidism (CH). Tg processing and secretion is controlled by extensive interactions with chaperone, trafficking, and degradation factors comprising the secretory proteostasis network. While dependencies on individual proteostasis network components are known, the integration of proteostasis pathways mediating Tg protein quality control and the molecular basis of mutant Tg misprocessing remain poorly understood. We employ a multiplexed quantitative affinity purification–mass spectrometry approach to define the Tg proteostasis interactome and changes between WT and several CH-variants. Mutant Tg processing is associated with common imbalances in proteostasis engagement including increased chaperoning, oxidative folding, and engagement by targeting factors for ER-associated degradation (ERAD). Furthermore, we reveal mutation-specific changes in engagement with N-glycosylation components, suggesting distinct requirements for one Tg variant on dual engagement of both oligosaccharyltransferase complex isoforms for degradation. Modulating dysregulated proteostasis components and pathways may serve as a therapeutic strategy to restore Tg secretion and thyroid hormone biosynthesis.

A struggling Detroit Lions offense awoke late, scoring 19-unanswered points to rally past the Houston Texans and improve to 8-1 this season. The Lions overcame a career-high five interceptions Jared Goff threw.