Loss of functional β-cell mass is an essential feature of type 2 diabetes, and maintaining mature β-cell identity is important for preserving a functional β-cell mass. However, it is unclear how β-cells achieve and maintain their mature identity. Here we demonstrate a novel function of protein arginine methyltransferase 1 (PRMT1) in maintaining mature β-cell identity. Prmt1 knockout in fetal and adult β-cells induced diabetes, which was aggravated by high-fat diet–induced metabolic stress. Deletion of Prmt1 in adult β-cells resulted in the immediate loss of histone H4 arginine 3 asymmetric dimethylation (H4R3me2a) and the subsequent loss of β-cell identity. The expression levels of genes involved in mature β-cell function and identity were robustly downregulated as soon as Prmt1 deletion was induced in adult β-cells. Chromatin immunoprecipitation sequencing and assay for transposase-accessible chromatin sequencing analyses revealed that PRMT1-dependent H4R3me2a increases chromatin accessibility at the binding sites for CCCTC-binding factor (CTCF) and β-cell transcription factors. In addition, PRMT1-dependent open chromatin regions may show an association with the risk of diabetes in humans. Together, our results indicate that PRMT1 plays an essential role in maintaining β-cell identity by regulating chromatin accessibility.

On the heels of the ADA’s December interim policy on vaping comes a new CDT code that references counseling services about high-risk substance use including vaping.

The Navy has been particularly hard hit by the coronavirus pandemic and is working to adapt its strategies for maintenance as well as containing outbreaks on ships.

Pediatric primary care has undergone a cultural shift. Changes in electronic health records, certification requirements, and practice structure have left many physicians feeling too busy to participate in research. Practice-based research networks must adapt to fit the current climate.

Adding quality improvement activities that meet Maintenance of Certification Part 4 criteria to research study design adds value to a practice-based research protocol. This incentive meets the needs of busy physicians, and may help researchers meet study recruitment goals. (Read the full article)

University officials, who have discouraged visits to campus and campus landmarks due to COVID-19, are increasing signage and social distancing guidance around the sites, as visitors continue to seek photographs in those areas and appear to be ignoring health care recommendations.

Today, many assets across multiple industries are becoming more instrumented and connected to enterprise platforms to provide additional insight into their health and operation. IDC estimates that Internet of Things (IoT) investment will reach $1.12 trillion in 2023. One important area for many industrial organizations that are focused in using [...]

An approach to enabling predictive maintenance for industrial assets was published on SAS Voices by Sanjeev Heda

Middletown --

Location: North Broad Street (Railroad Crossing) between Cedar Lane Road and Middletown Warwick Road/Summit Bridge Road, Middletown.

Times and Dates: 9:00 p.m. on Wednesday, March 18, 2020 until 5:00 a.m. on Thursday, April 2, 2020

Traffic Information: DelDOT announces to motorists that Delmarva Central Railroad will be replacing the railroad crossing, performing railroad signal upgrades, and doing general maintenance on their railroad crossing on North Broad Street. DelDOT's crews will be reconstructing the existing traffic signals. [More]

Houston --

DelDOT announces to motorists that Delmarva Central Railroad will be resurfacing and performing general maintenance on their railroad crossing at Deep Grass Lane between School Street and Front Street. The closure will begin at 5:00 a.m. on Friday, April 17, 2020 until 11:59 p.m. on Sunday, April 19, 2020.

Detour Routes:

Northbound: Deep Grass Lane onto Front Street to Broad Street to School Street and return to Deep Grass Lane. [More]

Seaford --

Seaford:

DelDOT announces to motorists that Delmarva Central Railroad will be resurfacing and performing general maintenance on their railroad crossing at Industrial Park Boulevard between Park Avenue and Ross Station Road.

The closure will begin at 5:00 a.m. on Wednesday, April 22, 2020 until 11:59 p.m. on Friday, April 24, 2020, pending weather.

Detour Routes:

Eastbound: Industrial Park Boulevard to Park Avenue onto Ross Station Road and return to Industrial Park Boulevard. [More]

Milford --

Location: US 113 Southbound (Railroad Crossing) between Route 14/Milford-Harrington Highway and East Lane, Milford.

Times and Date: 8:00 a.m. until 4:30 p.m. on Wednesday, April 15, 2020.

Traffic Information: DelDOT announces to motorists that Delmarva Central Railroad will be performing general maintenance on their railroad crossing on US 113 in Milford. [More]

Dover --

Location: Route 1 Northbound between Barkers Landing Road and Route 9, Dover.

Times & Dates: 9:00 a.m. until 1:00 p.m., Thursday, April 16, 2020, pending weather.

Traffic Information: DelDOT announces to motorists that the right lane and shoulder will be closed on Route 1 northbound for maintenance of the overhead message board on Route 1. [More]

Greenwood --

DelDOT announces to motorists that Delmarva Central Railroad will be resurfacing and performing general maintenance on their railroad crossing at Adams Road between Newton Road and US 13.

The closure will begin at 5:00 a.m. on Sunday, April 19, 2020 until 11:45 p.m. on Tuesday, April 21, 2020, pending weather.

Detour Routes:

Northbound: Adams Road on Newton Road to US 13 and return to Adams Road. [More]

Lincoln --

DelDOT announces to motorists that Delmarva Central Railroad will be resurfacing and performing general maintenance of their railroad crossing at Butler Avenue between North Old State Road and Front Street.

The closure will begin at 5:00 a.m. on Saturday, April 18, 2020 until 11:59 p.m. on Tuesday, April 21, 2020.

Detour Routes:

Eastbound: Butler Avenue onto North Old State Road to Johnson Road to Front Street and return to Butler Avenue. [More]

Blades --

DelDOT announces to motorists that Delmarva Central Railroad will be resurfacing and performing general maintenance on their railroad crossing on Johnson Road between Gum Branch Road and Seaford Road.

The closure will begin at 5:00 a.m. on Thursday, April 23, 2020 until 11:59 p.m. on Saturday, April 25, 2020, pending weather.

Detour Routes:

Eastbound: Johnson Road to Gum Branch Road onto O'Neals Road to Seaford Road and return to Johnson Road. [More]

Laurel --

DelDOT announces to motorists that Delmarva Central Railroad will be resurfacing and performing general maintenance on their railroad crossing on Old Sailor Road between Woodland Ferry Road and Penn Street.

The closure will begin at 5:00 a.m. on Tuesday, April 28, 2020 until 11:59 p.m. on Thursday, April 30, 2020, pending weather.

Detour Routes:

Eastbound: Old Sailor Road onto Woodland Ferry Road to Penn Street and return to Old Sailor Road. [More]

Laurel --

DelDOT announces to motorists that Delmarva Central Railroad will be resurfacing and performing general maintenance on their railroad crossing on Dukes Road between Hastings Drive and Bi State Boulevard.

The closure will begin at 5:00 a.m. on Monday, April 27, 2020 until 11:59 p.m. on Wednesday, April 29, 2020, pending weather.

Detour Routes:

Eastbound: Dukes Road onto Hastings Drive to West Street to Market Street to South Central Avenue and return to Dukes Road. [More]

Laurel --

DelDOT announces to motorists that Delmarva Central Railroad will be resurfacing and performing general maintenance on their railroad crossing on Market Street between Oak Street and Townsend Street.

The closure will begin at 9:00 a.m. on Thursday, April 30, 2020 until 11:59 p.m. on Tuesday, May 12, 2020, pending weather.

Detour Routes:

Vehicular Traffic:

Eastbound: West Street to West Sixth Street onto South Central Avenue and return to Market Street. [More]

Frankford --

DelDOT announces to motorists that Maryland and Delaware Railroad will be replacing, resurfacing and performing general maintenance on their crossing at Lazy Lagoon Road between US 113 and Pepper Road.

The road closure will begin at 5:00 a.m. on Thursday, May 14, 2020 until 11:59 p.m. on Thursday, May 21, 2020, pending weather.

Detour Routes:

Eastbound: Lazy Lagoon Road to US 113 to Delaware Avenue to Pepper Road and return to Lazy Lagoon Road. [More]

STRUCTURE MAINTENANCE, BR 1-400 ON SHALLCROSS LAKE ROAD

Central government funding has long been focused on social infrastructure such as schools and housing - with massive programmes of investment such as Building Schools for the Future. Infrastructure such as roads and bridges has not typically been fu...

In July 2017, the German Federal Cartel Office (“FCO”) published a detailed guidance paper (the “Guidance Paper”) on resale price maintenance (“RPM”) in the retail food sector. The purpose of the paper is to expl...

ABSTRACT

The protozoan parasites that cause malaria infect a wide variety of vertebrate hosts, including birds, reptiles, and mammals, and the evolutionary pressures inherent to the host-parasite relationship have profoundly shaped the genomes of both host and parasite. Here, we report that these selective pressures have resulted in unexpected alterations to one of the most basic aspects of eukaryotic biology, the maintenance of genome integrity through DNA repair. Malaria parasites that infect humans continuously generate genetic diversity within their antigen-encoding gene families through frequent ectopic recombination between gene family members, a process that is a crucial feature of the persistence of malaria globally. The continuous generation of antigen diversity ensures that different parasite isolates are antigenically distinct, thus preventing extensive cross-reactive immunity and enabling parasites to maintain stable transmission within human populations. However, the molecular basis of the recombination between gene family members is not well understood. Through computational analyses of the antigen-encoding, multicopy gene families of different Plasmodium species, we report the unexpected observation that malaria parasites that infect rodents do not display the same degree of antigen diversity as observed in Plasmodium falciparum and appear to undergo significantly less ectopic recombination. Using comparative genomics, we also identify key molecular components of the diversification process, thus shedding new light on how malaria parasites balance the maintenance of genome integrity with the requirement for continuous genetic diversification.

IMPORTANCE Malaria remains one of the most prevalent and deadly infectious diseases of the developing world, causing approximately 228 million clinical cases and nearly half a million deaths annually. The disease is caused by protozoan parasites of the genus Plasmodium, and of the five species capable of infecting humans, infections with P. falciparum are the most severe. In addition to the parasites that infect people, there are hundreds of additional species that infect birds, reptiles, and other mammals, each exquisitely evolved to meet the specific challenges inherent to survival within their respective hosts. By comparing the unique strategies that each species has evolved, key insights into host-parasite interactions can be gained, including discoveries regarding the pathogenesis of human disease. Here, we describe the surprising observation that closely related parasites with different hosts have evolved remarkably different methods for repairing their genomes. This observation has important implications for the ability of parasites to maintain chronic infections and for the development of host immunity.

ABSTRACT

The Candida albicans high-affinity phosphate transporter Pho84 is required for normal Target of Rapamycin (TOR) signaling, oxidative stress resistance, and virulence of this fungal pathogen. It also contributes to C. albicans’ tolerance of two antifungal drug classes, polyenes and echinocandins. Echinocandins inhibit biosynthesis of a major cell wall component, beta-1,3-glucan. Cells lacking Pho84 were hypersensitive to other forms of cell wall stress beyond echinocandin exposure, while their cell wall integrity signaling response was weak. Metabolomics experiments showed that levels of phosphoric intermediates, including nucleotides like ATP and nucleotide sugars, were low in pho84 mutant compared to wild-type cells recovering from phosphate starvation. Nonphosphoric precursors like nucleobases and nucleosides were elevated. Outer cell wall phosphomannan biosynthesis requires a nucleotide sugar, GDP-mannose. The nucleotide sugar UDP-glucose is the substrate of enzymes that synthesize two major structural cell wall polysaccharides, beta-1,3- and beta-1,6-glucan. Another nucleotide sugar, UDP-N-acetylglucosamine, is the substrate of chitin synthases which produce a stabilizing component of the intercellular septum and of lateral cell walls. Lack of Pho84 activity, and phosphate starvation, potentiated pharmacological or genetic perturbation of these enzymes. We posit that low substrate concentrations of beta-d-glucan- and chitin synthases, together with pharmacologic inhibition of their activity, diminish enzymatic reaction rates as well as the yield of their cell wall-stabilizing products. Phosphate import is not conserved between fungal and human cells, and humans do not synthesize beta-d-glucans or chitin. Hence, inhibiting these processes simultaneously could yield potent antifungal effects with low toxicity to humans.

IMPORTANCE Candida species cause hundreds of thousands of invasive infections with high mortality each year. Developing novel antifungal agents is challenging due to the many similarities between fungal and human cells. Maintaining phosphate balance is essential for all organisms but is achieved completely differently by fungi and humans. A protein that imports phosphate into fungal cells, Pho84, is not present in humans and is required for normal cell wall stress resistance and cell wall integrity signaling in C. albicans. Nucleotide sugars, which are phosphate-containing building block molecules for construction of the cell wall, are diminished in cells lacking Pho84. Cell wall-constructing enzymes may be slowed by lack of these building blocks, in addition to being inhibited by drugs. Combined targeting of Pho84 and cell wall-constructing enzymes may provide a strategy for antifungal therapy by which two sequential steps of cell wall maintenance are blocked for greater potency.

Maria Stager, Nathan R. Senner, Bret W. Tobalske, and Zachary A. Cheviron

Flexibility in heat generation and dissipation mechanisms provides endotherms the ability to match their thermoregulatory strategy with external demands. However, the degree to which these two mechanisms account for seasonal changes in body temperature regulation is little explored. Here we present novel data on the regulation of avian body temperature to investigate how birds alter mechanisms of heat production and heat conservation to deal with variation in ambient conditions. We subjected Dark-eyed Juncos (Junco hyemalis) to chronic cold acclimations of varying duration and subsequently quantified their metabolic rates, thermal conductance, and ability to maintain normothermia. Cold-acclimated birds adjusted traits related to both heat generation (increased summit metabolic rate) and heat conservation (decreased conductance) to improve their body temperature regulation. Increases in summit metabolic rate occurred rapidly, but plateaued after one week of cold exposure. In contrast, changes to conductance occurred only after nine weeks of cold exposure. Thus, the ability to maintain body temperature continued to improve throughout the experiment, but the mechanisms underlying this improvement changed through time. Our results demonstrate the ability of birds to adjust thermoregulatory strategies in response to thermal cues and reveal that birds may combine multiple responses to meet the specific demands of their environments.

Background

Previous studies showed that Cdc42, a member of the prototypical Rho family of small GTPases and a regulator of the actin cytoskeleton, is critical for the normal development and health of podocytes. However, upstream regulatory mechanisms for Cdc42 activity in podocytes are largely unknown.

High-quality tool kits come in a variety of sizes and styles for your home repair needs. Just a hammer and a flathead screwdriver won't cut it.

Kathryn Keneally, Assistant Attorney General for the Justice Department’s Tax Division, announced today the guilty plea of Thomas Nastasi III, 46, of Mt. Kisco, N.Y., to one count of willful failure to pay the Internal Revenue Service (IRS) the payroll taxes of his company, Nastasi Maintenance & Construction LLC.

John Mills and Terrance Allen, both of Malone, N.Y., pleaded guilty yesterday in federal court in Albany, N.Y., to conspiracy to violate the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) and substantive CERCLA counts in relation to the illegal removal, handling, and disposal of asbestos.

The Alliance met (virtually) on April 15 with Dr. Caitlin Boon, FDA Associate Commissioner for Food Policy and Response and several of her colleagues. She described CFSAN’s and CVM’s role in responding to the current pandemic, as well as how the agency is assuring a continued safe food supply. About 50% of food spending in the […]

Yokogawa Electric Corporation (TOKYO: 6841) and MetaMoJi Corporation announce that they have jointly developed SensPlus Note, a low cost and easy to implement solution for the digitization of plant data on mobile devices. SensPlus Note, a solution in Yokogawa's OpreX Operation and Maintenance Improvement family, improves the efficiency and quality of maintenance work and the precision of post-maintenance analyses by enabling data from plant field work to be used more efficiently. This solution will be released in all markets worldwide on January 31.

For all those of you who insist on green lawns, for your kids to play on or your dogs to roll in, you still don't have to lay down a carpet of thirsty bluegrass, which 95% of American lawns

State Farm: Spring Home Maintenance Tips

An aerial view of your mowing path helps you remember to switch up your pattern for optimum turf health.

Be Prepared! Protect your home from sudden heating repairs with a home warranty from AHS this winter. Home warranties help homeowners control repair or replacement costs during a break down.

An aerial view of your mowing path helps you remember to switch up your pattern for optimum turf health.

An aerial view of your mowing path helps you remember to switch up your pattern for optimum turf health.

Company: Nanu India Recruitment Co
Qualification: Bachelor Of Technology (B.Tech/B.E), Diploma
Experience: 0 to 2
Salary: 1.80 to 4.50
location: Chandigarh
Ref: 24818666
Summary: We have New Openings For Junior Engineer Operation and Maintenance. Freshers also can apply as Trainee.

Company: Ambe Consultancy Services Private Limited
Experience: 5 to 15
location: Gulf
Ref: 24816330

Company: Resource Access Management Solutions Private Limited
Experience: 3 to 7
location: Bengaluru / Bangalore
Ref: 24410363
Summary: JOB DESCRIPTION ORGANIZATION WRITE-UP The client company is a leading research-based biopharmaceutical company. We apply science and our global resources to deliver innovative therapies that....

Company: Nanu India Recruitment Co
Qualification: Bachelor Of Technology (B.Tech/B.E), Diploma
Experience: 0 to 2
Salary: 1.80 to 4.50
location: Mumbai, Pune
Ref: 24825985
Summary: Hiring For Mechanical Maintenance Engineer- Apply Now Freshers also can apply as Trainee.

Company: Nanu India Recruitment Co
Qualification: Bachelor Of Technology (B.Tech/B.E)
Experience: 0 to 2
Salary: 1.80 to 4.50
location: Delhi, Gurgaon / Gurugram
Ref: 24825980
Summary: We have Requirement for Instrumentation Maintenance Engineer- Apply Now. Freshers also can apply as Trainee.

Company: Nanu India Recruitment Co
Qualification: Bachelor Of Technology (B.Tech/B.E), Diploma
Experience: 0 to 2
Salary: 1.80 to 4.50
location: Ahmedabad, Chennai
Ref: 24824743
Summary: We have Requirement for Production & Maintenance Engineer. Freshers also can apply as Trainee.

Company: Confidential
Qualification: Bachelor of Arts (B.A), Bachelor of Architecture (B.Arch), Bachelor of Business Administration (B.B.A), Bachelor Of Computer Application (B.C.A), Bachelor of Commerce (B.Com), Bachelor Of Technology (B.Tech/B.E), Bachelor of Education (B.Ed), Bachelor of Science (B.Sc), 12th Class....

Company: Confidential
Qualification: Bachelor of Arts (B.A), Bachelor of Architecture (B.Arch), Bachelor of Business Administration (B.B.A), Bachelor Of Computer Application (B.C.A), Bachelor of Commerce (B.Com), Bachelor Of Technology (B.Tech/B.E), Bachelor of Education (B.Ed), Bachelor of Science (B.Sc), 12th Class....

Company: Confidential
Qualification: Bachelor of Arts (B.A), Bachelor of Architecture (B.Arch), Bachelor of Business Administration (B.B.A), Bachelor Of Computer Application (B.C.A), Bachelor of Commerce (B.Com), Bachelor Of Technology (B.Tech/B.E), Bachelor of Education (B.Ed), Bachelor of Science (B.Sc), 12th Class....

GDP From Services in Peru increased to 15557 PEN Million in the fourth quarter of 2019 from 14535 PEN Million in the third quarter of 2019. GDP From Services in Peru averaged 11663.48 PEN Million from 2007 until 2019, reaching an all time high of 15557 PEN Million in the fourth quarter of 2019 and a record low of 7351 PEN Million in the first quarter of 2007. This page provides - Peru Gdp From Services- actual values, historical data, forecast, chart, statistics, economic calendar and news.

The time to start a cleaning company is now.

Quotation - dated 11.10.2011 - Comprehensive Annual Maintenance Contract of Duplo make copy printer model No.31S of the Ministry of Corporate Affairs