maintenance The care and maintenance of a cast iron skillet By www.mnn.com Published On :: Sat, 11 Jun 2016 16:15:00 +0000 There are tricks to properly cleaning a cast iron skillet without ruining its seasoning. This infographic offers several. Full Article Healthy Eating
maintenance Nine Built-in Counters/Timers to measure equipment operating cycles and times and forecast maintenance timing By www.ia.omron.com Published On :: Tue, 16 Oct 2012 23:47:40 +0900 Topics Full Article Topics
maintenance Why Commercial Kitchen Maintenance Matters By rssnewsfeed.net Published On :: Wed, 19 Feb 2014 13:18:50 +0000 Twitter Read more here. For more about this, go here. Keywords: Our systems all conform to bs6173, Commercial kitchen wall cladding, Commercial refrigeration equipment, Gas interlock system, Commercial dishwasher repairs, Commercial refrigeration equipment. The post Why Commercial Kitchen Maintenance Matters appeared first on RSS News Feed. Full Article Food and Drink
maintenance The Three Top Reasons to Invest in the Best Forklift Battery Handling and Maintenance Equipment By rssnewsfeed.net Published On :: Mon, 13 Oct 2014 06:51:30 +0000 Twitter Check out this website for more. Check out this site for more. Keywords: Material handling equipment, Battery room best practices, Traveling battery transporter, Battery service, Power drive gantry cranes, Forklift battery safety. Graphics: Post This Visual The post The Three Top Reasons to Invest in the Best Forklift Battery Handling and Maintenance Equipment appeared first on RSS News Feed. Full Article Business
maintenance Regular and Preventative Air Conditioning Maintenance Can Save Homeowners Money By www.24-7pressrelease.com Published On :: Mon, 29 Apr 2019 07:00:00 GMT Homeowners can extend the lifespan, prevent costly repairs and improve energy efficiency of their air conditioners with frequent, preventative maintenance. Full Article
maintenance Imperial Landscaping Provides Complete Lawn Maintenance Packages to Commercial and Residential Clients in the Winston Salem and Surrounding Area By www.24-7pressrelease.com Published On :: Mon, 10 Jun 2019 07:00:00 GMT At Imperial Landscaping, we understand that the outdoor appearance of your business or home is as important as your interior. We have a team of lawn care specialists that will consult with you to determine your exact needs. Full Article
maintenance MPulse Software Makes Single Sign-On Easy for Maintenance Management Software Customers By www.24-7pressrelease.com Published On :: Tue, 04 Sep 2018 07:00:00 GMT Company Launches Single-Sign-On (SSO)/Active Directory Integration for Cloud-Hosted Customers Full Article
maintenance Your Spring Auto Maintenance Checklist From Delta Auto Protect By www.24-7pressrelease.com Published On :: Fri, 27 Apr 2018 07:00:00 GMT Cleaning off all of the salt and dirt will help keep your car looking fresh, but with only a little more work you can make sure that your car will be running well too. Full Article
maintenance Important Summer Auto Maintenance Tips From Delta Auto Protect By www.24-7pressrelease.com Published On :: Thu, 05 Jul 2018 07:00:00 GMT We here at Delta Auto Protect see our fair share of car trouble that could have been prevented with a bit of routine maintenance, so we put together this list of maintenance tips that can prevent your car from any unnecessary trips to the mechanic. Full Article
maintenance Impact Introduces the BinTracker Container Repair & Maintenance App By www.24-7pressrelease.com Published On :: Fri, 03 May 2019 07:00:00 GMT Haulers Can Manage Their Container Inventory on Their Phone Full Article
maintenance 4 Eco Services Kick Off to Preseason HVAC Maintenance By www.24-7pressrelease.com Published On :: Tue, 12 Mar 2019 07:00:00 GMT 4 Eco Services shares new details about their 2019 preseason maintenance campaign for HVAC system inspections. Full Article
maintenance 4 Eco Services Announces Initiative in the Greater Kansas Area on Sump Pump Maintenance By www.24-7pressrelease.com Published On :: Fri, 05 Apr 2019 07:00:00 GMT 4 Eco Services announced a promotion offering a $50 credit towards sump pump maintenance to help homeowners prevent flooding. Full Article
maintenance 4 Eco Services Announces A Promotion For Toilet Maintenance By www.24-7pressrelease.com Published On :: Wed, 07 Aug 2019 07:00:00 GMT 4 Eco Services, a local plumbing, heating and air conditioning service company, is offering a 10-point toilet tune up promotion to help homeowners keep their unit in optimal condition and reduce costly water waste. Full Article
maintenance Delta Auto Protect's Essential Road Trip Maintenance Checklist By www.24-7pressrelease.com Published On :: Fri, 10 Aug 2018 07:00:00 GMT Many common automotive issues that can arise when out on the road could be prevented with a bit of maintenance before the trip. That's why we at Delta Auto Protect packaged this checklist you can use before heading out on that road trip. Full Article
maintenance Imperial Landscaping of Winston Salem, an Award Winning, Customer Service Driven Landscaping and Lawn Care Company is Now Accepting New Lawn Maintenance Customers By www.24-7pressrelease.com Published On :: Sun, 03 May 2020 07:00:00 GMT A strong commitment to customer satisfaction and service is why Imperial Landscaping continues to receive Outstanding Recognition among landscaping and lawn care companies. Full Article
maintenance Junior Arena Maintenanceperson EX20-035 (Recruitment Pool) By www.greatersudbury.ca Published On :: Fri, 17 Apr 2020 18:33:38 GMT Full Article Collections Elections 2014 Engagement Leisure Adult Ages 13 to 18 Ages 6 to 12 Birth to Age 5 Senior Newsroom Mastheads Backgrounder Emergency Advisory Emergency Bulletin Emergency Warning Media Advisory News Release Public Service Announcement Traffic Report Newsroom Streams City Hall Move Play Prepare Work Video Archives Upcoming Event
maintenance Tim Jarrell Named Chief of Maintenance at Grand Canyon National Park By www.nps.gov Published On :: Wed, 18 Mar 2009 20:00:00 EST Tim Jarrell, a 32 year veteran of the National Park Service, was recently named Chief of the Facilities Management Division at Grand Canyon National Park. https://www.nps.gov/grca/learn/news/3009-03-19-jarrell.htm Full Article
maintenance Repair and Maintenance to Temporarily Close Ribbon Falls Bridge By www.nps.gov Published On :: Tue, 31 Jan 2012 04:11:00 EST Grand Canyon National Parkâs Trail Crew will soon begin repairs to the bridge across Bright Angel Creek on the Ribbon Falls spur trail in the Inner Canyon. https://www.nps.gov/grca/learn/news/2012-01-31_bridge.htm Full Article
maintenance Repair and Maintenance to Temporarily Close Walkway between Train Depot and El Tovar Hotel By www.nps.gov Published On :: Wed, 24 Jun 2015 07:20:00 EST The trail between the El Tovar Hotel and the train depot will close for repairs July 1. https://www.nps.gov/grca/learn/news/el-tovar-walkway-detour.htm Full Article
maintenance Grand Canyon National Park to Temporarily Close Colorado River Trail for Maintenance Starting Monday, April 3 By www.nps.gov Published On :: Fri, 31 Mar 2017 08:22:00 EST Due to an unanticipated rockslide the National Park Service (NPS) will close the Colorado River Trail between Pipe Creek and the Silver Bridge for trail repair starting Monday, April 3. https://www.nps.gov/grca/learn/news/repairs-close-river-trail.htm Full Article
maintenance Grand Canyon National Park Initiates Water Conservation Measures for Maintenance By www.nps.gov Published On :: Fri, 06 Dec 2019 08:38:00 EST Grand Canyon National Park is initiating water conservation measures for the South Rim, including Desert View, from Dec. 9-19, 2019, for scheduled maintenance at the Indian Garden North pump house. https://www.nps.gov/grca/learn/news/grand-canyon-initiates-water-conservation-measures-for-maintenance-20191206.htm Full Article
maintenance Escaping the maintenance mode trap By wpengineer.com Published On :: Mon, 06 Jan 2014 10:00:13 +0000 WordPress makes upgrading very easy . You simply click “Update now”, wait for a minute or two and your system […] Full Article WordPress Tutorials Maintenance Mode upgrade
maintenance Apparatus for holding a ski or snowboard during repair and maintenance By www.freepatentsonline.com Published On :: Tue, 14 Apr 2015 08:00:00 EDT A ski tip hold down assembly for holding the tip end of the ski. The ski tip hold down assembly having a base attached to a work bench and an upright stand affixed to the base. The upright stand having a ski rest on a top surface thereof and a ski tip hold down bar secured to the upright stand in spaced apart relation to the ski rest defining an opening adapted to receive a ski tip, with the ski oriented in a base-up configuration, with the ski tip extending underneath the ski tip hold down bar. The apparatus further including a tail rest assembly having a base adapted to be used on the work bench in spaced apart relation to the ski tip hold down assembly and further including an upright stand affixed to the base with a ski rest provided on a top surface thereof. Full Article
maintenance Automating predictive maintenance for automobiles By www.freepatentsonline.com Published On :: Tue, 26 May 2015 08:00:00 EDT An approach is provided to automate predictive vehicle maintenance. In the approach, a vehicle's information handling system receives vehicle data transmissions from a number of other vehicles in geographic proximity to the vehicle. Both the vehicle and the other vehicles correspond to various vehicle types that are used to identify those other vehicles that are similar to the vehicle. The sets of received vehicle data transmissions that are received to similar vehicles are analyzed with respect to a plurality of vehicle maintenance data corresponding to the vehicle. The analysis of the vehicle data transmissions resulting in predictive vehicle maintenance recommendations pertaining to the first vehicle. Full Article
maintenance Methods and systems for global knowledge sharing to provide corrective maintenance By www.freepatentsonline.com Published On :: Tue, 26 May 2015 08:00:00 EDT Described herein are methods and systems for providing corrective maintenance using global knowledge sharing. A method to provide corrective maintenance with a CM system includes performing a query to generate a ranking of fixable causes based on factors (e.g., symptoms, configuration, test). The ranking may be determined based on a fixable cause percent match with the factors. The ranking of fixable causes may be associated with one or more solutions for each fixable cause. The ranking can be updated based on performing tests or solutions. Full Article
maintenance DYNAMIC RECONNECT OF WORK PROCESSES IN A ZERO DOWNTIME MAINTENANCE PROCEDURE By www.freepatentsonline.com Published On :: Thu, 29 Jun 2017 08:00:00 EDT The disclosure generally describes methods, software, and systems, including a method for updating an application. At least one application instance of an application is managed. Each application instance is associated with a plurality of executing work processes connected with a first database schema. A bridge database schema is generated that is related to the first database schema. The bridge database schema represents a copy of the first database schema and is generated in response to initiation of an update to the application. In response to determining that the generation of the bridge database schema is complete, for each of the plurality of work processes, a commit work action performed by the particular work process is determined. In response to determining performance of the commit work action, the particular work process is connected to the bridge database schema. Full Article
maintenance SYSTEM AND METHOD FOR IMPROVED HEALTH MANAGEMENT AND MAINTENANCE DECISION SUPPORT By www.freepatentsonline.com Published On :: Thu, 29 Jun 2017 08:00:00 EDT A method of health management of a monitored system includes collecting component condition indicator data used to calculate a plurality of component health indicators. Component fault severity and potential failure modes are determined utilizing the component condition indicator data. The potential failure modes are ranked in order of likelihood to isolate the failure mode. A system of health management for monitored apparatus includes a fault severity module to derive a plurality of component health indicators from collected component condition indicator data, the plurality of component health indicators indicative of fault severity of a plurality of components. A fault isolation module separately derives a ranked listing of potential fault/failure modes utilizing the component condition indicator data. The system further includes a communication portion to which the plurality of component health indicators and the ranked listing of fault/failure modes are transmitted for review and/or action by cognizant personnel. Full Article
maintenance Aircraft turbojet engine nacelle air intake maintenance trolley By www.freepatentsonline.com Published On :: Tue, 12 May 2015 08:00:00 EDT This maintenance trolley (17) for the air intake (9) of the nacelle of an aircraft turbojet engine is notable in that it comprises a base (19) and a platform (23) cantilever-mounted on this base (19) and designed to enter said air intake (9) without contact therewith. Full Article
maintenance REMOTE MAINTENANCE SYSTEM By www.freepatentsonline.com Published On :: Thu, 29 Jun 2017 08:00:00 EDT A remote maintenance system includes a maintenance management apparatus connected to a user apparatus and a communication relay apparatus connected to a remote-controlled apparatus. The maintenance management apparatus transmits, to the communication relay apparatus, message data whose destination is unique identification information of a mobile communication network assigned in advance to the communication relay apparatus. The communication relay apparatus notifies the maintenance management apparatus of an IP address that is dynamically assigned to itself upon reception of the message data. The maintenance management apparatus transmits, to the communication relay apparatus, information on remote operation received from the user apparatus whose destination is the notified IP address. The communication relay apparatus relays, to the remote-controlled apparatus, the information on the remote control received from the maintenance management apparatus. Full Article
maintenance Maintenance Decision Support System and Method for Vehicular and Roadside Applications By www.freepatentsonline.com Published On :: Thu, 29 Jun 2017 08:00:00 EDT A method and system are provided in which maintenance vehicles collect information from sensors and operators, forward the collected information to a server, and, is response, receive maps and operator instructions. Full Article
maintenance PREDICTION OF COMPONENT MAINTENANCE By www.freepatentsonline.com Published On :: Thu, 13 Apr 2017 08:00:00 EDT One or more processors determine wear for a robotic device. The one or more processors divide a direction of travel of a robotic device into zones. Each zone has an associated counter that counts how many times the robotic device has entered a given zone. The one or more processors update a count for a zone in response to the robotic device entering that zone. The one or more processors determine a level of wear for a component associated with the robotic device. The level of wear is based, at least in part, on a total of counts for the zones. Full Article
maintenance Multifunctional tool for automotive air conditioning system testing and maintenance By www.freepatentsonline.com Published On :: Tue, 28 Oct 2014 08:00:00 EDT An automotive tool for servicing air-conditioning (AC) systems equipped with quick-connect adaptors. May be used to service modern refrigerant R132A-compatible systems or retrofitted older systems. The tool is adapted for attaching, detaching, opening, and closing valved T-adaptors used to attach gas lines and pressure gauges such as required for adjusting pressure in AC systems at full operational pressure and temperature, and particularly finds use when the “hi-tap” and “lo-tap” fittings are in hard to reach places around the engine block or chassis. The tool includes swappable extension arms so that length and angular geometry may be adjusted as needed. The tool aids in avoiding injury on dangerously hot surfaces and moving fan blades or belts. Surprisingly, using two such tools, one for the high tap and one for the low tap, a mechanic may work each tool singlehandedly to complete any necessary pressure adjustments. Full Article
maintenance ROTARY MACHINE, METHOD OF ASSEMBLING ROTARY MACHINE, AND METHOD OF PERFORMING MAINTENANCE OF ROTARY MACHINE By www.freepatentsonline.com Published On :: Thu, 29 Jun 2017 08:00:00 EDT In a rotary machine, a method of assembling a rotary machine, and a method of performing maintenance of a rotary machine, a combustor casing as an outer casing configured from upper and lower casings forming a half split shape, an intermediate shaft cover as an inner casing configured from upper and lower casings forming a half split shape and supporting a combustor in the combustor casing, and a pressing support mechanism that presses and supports a portion to be fit of the combustor casing and a fitting portion of the intermediate shaft cover in an axial direction of a rotor are provided, and thus, detachment of the casing is made easy and maintainability of an inside thereof is improved in the method of performing maintenance of a rotary machine. Full Article
maintenance In response to West Seattle Bridge closure, city to audit maintenance and spending on its aging bridges By www.seattletimes.com Published On :: Thu, 23 Apr 2020 15:43:13 -0700 Last month’s emergency shutdown of the West Seattle Bridge has prompted Seattle City Council member Alex Pedersen to request an audit of the city’s 124 bridges, he said Thursday. Pedersen, who chairs the council’s Transportation and Utilities Committee, said City Auditor David G. Jones will compile data about maintenance spending and comparisons to peer cities. […] Full Article Local News Puget Sound Traffic Lab
maintenance Documents reveal maintenance of Collins Class submarines could be scrapped from SA by 2024 By www.abc.net.au Published On :: Fri, 04 Oct 2019 18:51:00 +1000 Full-cycle docking of Collins Class submarines could disappear from its current site in South Australia by 2024, Freedom of Information (FOI) documents reveal. Full Article ABC Radio Adelaide adelaide perth Business Economics and Finance:All:All Business Economics and Finance:Industry:All Business Economics and Finance:Industry:Defence and Aerospace Industries Defence and National Security:All:All Defence and National Security:Defence Industry:All Government and Politics:All:All Government and Politics:Federal - State Issues:All Government and Politics:Federal Government:All Government and Politics:States and Territories:All Australia:All:All Australia:SA:Adelaide 5000 Australia:SA:All Australia:SA:Osborne 5017 Australia:WA:All Australia:WA:Henderson 6166 Australia:WA:Perth 6000
maintenance Senate inquiry following Angel Flight crashes urges CASA to relax maintenance standards By www.abc.net.au Published On :: Mon, 07 Oct 2019 13:03:00 +1100 The aviation safety regulator is being urged to relax maintenance requirements for Angel Flight and other charity flight services, after a Senate inquiry slammed an earlier ATSB report into the charity's fatal crash rate. Full Article ABC Radio Adelaide adelaide southeastsa westernvic melbourne Disasters and Accidents:Accidents:Air and Space Disasters and Accidents:Accidents:All Disasters and Accidents:All:All Government and Politics:All:All Government and Politics:Parliament:All Government and Politics:Parliament:Federal Parliament Australia:All:All Australia:SA:Adelaide 5000 Australia:SA:All Australia:SA:Mount Gambier 5290 Australia:VIC:All Australia:VIC:Horsham 3400 Australia:VIC:Melbourne 3000
maintenance The United Coal Mine entered care and maintenance in March 2010, while the Wambo Coal Mine has been in operation for nearly 50 years. By www.abc.net.au Published On :: Thu, 29 Aug 2019 19:13:00 +1000 Full Article 1233 ABC Newcastle newcastle Business Economics and Finance:Industry:Mining Rural:Mining:All Australia:NSW:Warkworth 2330
maintenance Mejia v. Merchants Building Maintenance By feeds.findlaw.com Published On :: 2019-08-13T08:00:00+00:00 (California Court of Appeal) - Affirmed denial of motion to compel arbitration. An employee bringing a Private Attorney General’s Act claim may not be compelled to arbitrate that portion of the claim that seeks to recover underpaid wages. Full Article Dispute Resolution & Arbitration Labor & Employment Law
maintenance Mejia v. Merchants Building Maintenance By feeds.findlaw.com Published On :: 2019-08-13T08:00:00+00:00 (California Court of Appeal) - Affirmed denial of motion to compel arbitration. An employee bringing a Private Attorney General’s Act claim may not be compelled to arbitrate that portion of the claim that seeks to recover underpaid wages. Full Article Dispute Resolution & Arbitration Labor & Employment Law
maintenance Existing and Emerging Highway Infrastructure Preservation, Maintenance, and Renewal Definitions, Practices, and Scenarios By www.trb.org Published On :: Thu, 23 Apr 2020 13:45:15 GMT The National Cooperative Highway Research Program's NCHRP Web-Only Document 272: Existing and Emerging Highway Infrastructure Preservation, Maintenance, and Renewal Definitions, Practices, and Scenarios provides appendices to NCHRP Report 750, Volume 7: Preservation, Maintenance, andRenewal of Highway Infrastructure . Full Article
maintenance Guidebook for Advanced Computerized Maintenance Management System Integration at Airports By www.trb.org Published On :: Mon, 12 Mar 2018 16:38:43 GMT TRB's Airport Cooperative Research Program (ACRP) Research Report 155: Guidebook for Advanced Computerized Maintenance Management System Integration at Airports explores the use of a Computerized Maintenance Management System (CMMS) to manage a variety of assets across a number of different airport systems. This report develops guidance on the steps necessary to implement a CMMS, factors for consideration in prioritizing which systems should be included in the CMMS using a phased approach, and the steps ... Full Article http://www.trb.org/Resource.ashx?sn=acrp_rpt_155cover
maintenance Maintenance cost savings for pavement surface treatments By www.trb.org Published On :: Thu, 30 Apr 2020 05:16:46 GMT Use of Surface Treatments to Extend Pavement Life: A Case Study on US 301, Sussex County, Virginia , released by the Virginia Department of Transportation Full Article
maintenance Mature myelin maintenance requires Qki to coactivate PPARβ-RXRα–mediated lipid metabolism By www.jci.org Published On :: Lipid-rich myelin forms electrically insulating, axon-wrapping multilayers that are essential for neural function, and mature myelin is traditionally considered metabolically inert. Surprisingly, we discovered that mature myelin lipids undergo rapid turnover, and quaking (Qki) is a major regulator of myelin lipid homeostasis. Oligodendrocyte-specific Qki depletion, without affecting oligodendrocyte survival, resulted in rapid demyelination, within 1 week, and gradually neurological deficits in adult mice. Myelin lipids, especially the monounsaturated fatty acids and very-long-chain fatty acids, were dramatically reduced by Qki depletion, whereas the major myelin proteins remained intact, and the demyelinating phenotypes of Qki-depleted mice were alleviated by a high-fat diet. Mechanistically, Qki serves as a coactivator of the PPARβ-RXRα complex, which controls the transcription of lipid-metabolism genes, particularly those involved in fatty acid desaturation and elongation. Treatment of Qki-depleted mice with PPARβ/RXR agonists significantly alleviated neurological disability and extended survival durations. Furthermore, a subset of lesions from patients with primary progressive multiple sclerosis were characterized by preferential reductions in myelin lipid contents, activities of various lipid metabolism pathways, and expression level of QKI-5 in human oligodendrocytes. Together, our results demonstrate that continuous lipid synthesis is indispensable for mature myelin maintenance and highlight an underappreciated role of lipid metabolism in demyelinating diseases. Full Article
maintenance Public Works Maintenance & Operations Manager By www.engineer.net Published On :: Thu, 27 Jul 2017 00:00:00 UTC The City of Glendora seeks a strong manager with a proven background as a leader to raise the bar in the Maintenance Services Division of our Public Works Department. This position oversees streets, traffic, storm drains, equipment, facilities and related operations including fleet maintenance. Re Full Article
maintenance Facility Maintenance Supervisor By www.engineer.net Published On :: Thu, 02 Nov 2017 00:00:00 UTC Job Overview: Supervises and is responsible for the management of building and equipment maintenance personnel, on all shifts to ensure the facility and equipment are maintained at the highest level of efficiency. Job Responsibilities and Accountabilities: Supervises and trains maintenanc Full Article
maintenance Confederation Line out of service for maintenance work By ottawa.ctvnews.ca Published On :: Sat, 9 May 2020 04:00:00 -0400 The City of Ottawa says the Confederation Line will be shutdown from Saturday, May 9 to Wednesday, May 13 so Rideau Transit Group can conduct maintenance work. Full Article
maintenance Role of liver in the maintenance of cholesterol and low density lipoprotein homeostasis in different animal species, including humans By feedproxy.google.com Published On :: 1993-10-01 JM DietschyOct 1, 1993; 34:1637-1659Reviews Full Article
maintenance Role of Physical Activity for Weight Loss and Weight Maintenance By spectrum.diabetesjournals.org Published On :: 2017-08-01 Carla E. CoxAug 1, 2017; 30:157-160From Research to Practice Full Article
maintenance Adipose Triglyceride Lipase is a Key Lipase for the Mobilization of Lipid Droplets in Human Beta Cells and Critical for the Maintenance of Syntaxin1a Level in Beta Cells By diabetes.diabetesjournals.org Published On :: 2020-03-31T14:12:19-07:00 Lipid droplets (LDs) are frequently increased when excessive lipid accumulation leads to cellular dysfunction. Distinct from mouse beta cells, LDs are prominent in human beta cells, however, the regulation of LD mobilization (lipolysis) in human beta cells remains unclear. We found that glucose increases lipolysis in non-diabetic human islets, but not in type 2 diabetic (T2D) islets, indicating dysregulation of lipolysis in T2D islets. Silencing adipose triglyceride lipase (ATGL) in human pseudoislets (shATGL) increased triglycerides, and the number and size of LDs indicating that ATGL is the principal lipase in human beta cells. In shATGL pseudoislets, biphasic glucose-stimulated insulin secretion (GSIS) and insulin secretion to IBMX and KCl were all reduced without altering oxygen consumption rate compared with scramble control. Like human islets, INS1 cells showed visible LDs, glucose responsive lipolysis, and impairment of GSIS after ATGL silencing. ATGL deficient INS1 cells and human pseudoislets showed reduced Stx1a, a key SNARE component. Proteasomal degradation of Stx1a was accelerated likely through reduced palmitoylation in ATGL deficient INS1 cells. Therefore, ATGL is responsible for LD mobilization in human beta cells and supports insulin secretion by stabilizing Stx1a. The dysregulated lipolysis may contribute to LD accumulation and beta cell dysfunction in T2D islets. Full Article
maintenance Adipose Triglyceride Lipase is a Key Lipase for the Mobilization of Lipid Droplets in Human Beta Cells and Critical for the Maintenance of Syntaxin1a Level in Beta Cells By diabetes.diabetesjournals.org Published On :: 2020-04-20T08:51:08-07:00 Lipid droplets (LDs) are frequently increased when excessive lipid accumulation leads to cellular dysfunction. Distinct from mouse beta cells, LDs are prominent in human beta cells, however, the regulation of LD mobilization (lipolysis) in human beta cells remains unclear. We found that glucose increases lipolysis in non-diabetic human islets, but not in type 2 diabetic (T2D) islets, indicating dysregulation of lipolysis in T2D islets. Silencing adipose triglyceride lipase (ATGL) in human pseudoislets (shATGL) increased triglycerides, and the number and size of LDs indicating that ATGL is the principal lipase in human beta cells. In shATGL pseudoislets, biphasic glucose-stimulated insulin secretion (GSIS) and insulin secretion to IBMX and KCl were all reduced without altering oxygen consumption rate compared with scramble control. Like human islets, INS1 cells showed visible LDs, glucose responsive lipolysis, and impairment of GSIS after ATGL silencing. ATGL deficient INS1 cells and human pseudoislets showed reduced Stx1a, a key SNARE component. Proteasomal degradation of Stx1a was accelerated likely through reduced palmitoylation in ATGL deficient INS1 cells. Therefore, ATGL is responsible for LD mobilization in human beta cells and supports insulin secretion by stabilizing Stx1a. The dysregulated lipolysis may contribute to LD accumulation and beta cell dysfunction in T2D islets. Full Article