Method is possible replacement for phosphoramidite-based DNA synthesis

Una estructura sintética podría reemplazar algún día las partes dañadas por enfermedad.

合成的结构体有朝一日或可替代疾病中受损的结构体

Synthetic construct could someday replace damaged counterparts in disease

Shiv Sena (UBT) posted a video of the poll authorities' act on its X handle.

Former captain Paul O'Connell says it will be vital for the British & Irish Lions to pick a leader capable of beating the Springboks at their own game.

The following article, Mark Levin on Michael Flynn Bombshell Documents: This Is “Barack Obama’s Blue Dress” Without The DNA, was first published on 100PercentFedUp.com.

Mark Levin nailed the importance of the newly released documents that cleared Michael Flynn and pinned Obama to the wall. He says that the documents are “Barack Obama’s Blue Dress” without the “DNA” alluding to the blue dress from Monica Lewinsky that proved Bill Clinton’s guilt. Levin begins by praising AG Bill Barr and then […]

Continue reading: Mark Levin on Michael Flynn Bombshell Documents: This Is “Barack Obama’s Blue Dress” Without The DNA ...

Workers in the OM Near East Field structure a new team around church planting principles.

Officials in Italy say an Italian aid worker who was kidnapped in Kenya in late 2018 has been freed in Somalia

Western African maritime regions are prone to pirate activity, and it is expected that the number of incidents, notably those involving kidnapping for ransom, will increase in upcoming years. ......

The Rivers State Police Command has rescued a Lebanese, Bilal Nosser from a kidnappers’ den in Ndashi forest, Etche Local Government Area of the state. Bilal, popularly known as Mister Bee is said to be a renowned Nollywood actor, who was kidnapped in the early hours of Friday in Imo State and taken to a […]

Police rescue popular Nollywood actor from kidnappers’ den

Silvia Romano was 23 and working as a volunteer in the orphanage in Chakama village in southeast Kenya when she was seized by gunmen in November 2018

Italian PM says the volunteer, who was taken from Kilifi in 2018, has been released.

Will speedy DNA tests help convict violent offenders or curb privacy?

ROME: A young Italian woman who was kidnapped in late 2018 from an orphanage in Kenya, has been freed, Prime Minister Giuseppe Conte announced on Saturday.Silvia Romano was 23 and working as a...

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ROME: A young Italian woman who was kidnapped in late 2018 from an orphanage in Kenya, has been freed, Prime Minister Giuseppe Conte announced on Saturday.Silvia Romano was 23 and working as a volunteer in the orphanage in Chakama village in southeast Kenya when she was seized by gunmen in...

Greg James missed his second Radio 1 show on Thursday after being 'kidnapped' from a BRIT Awards after-party on Tuesday as part of an elaborate stunt.

Minister for Industries, mines and parliamentary affairs Prakash Mehta will attend the programmes regarding 26/11 on behalf of chief minister Devendra Fadnavis.

CM has assured safe passage for migrant workers, says Sharad Pawar

Arti Singh remembered her first letter to mother on the occasion of Mothers' Day

The coronavirus lockdown has separated many of us from our families, including our mothers. However, Sophie Choudhry is one lucky celeb who is able to spend her quarantine and Mothers' Day with her supermom. In fact, she told us all about her plans for the day.

The structure and function of dioxygenases in histone demethylation and DNA/RNA dimethylation are discussed.

'Rumbling' felt during Ardnamurchan earthquake  BBC News

TatD has been thoroughly investigated as a DNA-repair enzyme and an apoptotic nuclease, and still-unknown TatD-related DNases are considered to play crucial cellular roles. However, studies of TatD from Gram-positive bacteria have been hindered by an absence of atomic detail and the resulting inability to determine function from structure. In this study, an X-ray crystal structure of SAV0491, which is the TatD enzyme from the Gram-positive bacterium Staphylococcus aureus (SaTatD), is reported at a high resolution of 1.85 Å with a detailed atomic description. Although SaTatD has the common TIM-barrel fold shared by most TatD-related homologs, and PDB entry 2gzx shares 100% sequence identity with SAV0491, the crystal structure of SaTatD revealed a unique binding mode of two phosphates interacting with two Ni2+ ions. Through a functional study, it was verified that SaTatD has Mg2+-dependent nuclease activity as a DNase and an RNase. In addition, structural comparison with TatD homologs and the identification of key residues contributing to the binding mode of Ni2+ ions and phosphates allowed mutational studies to be performed that revealed the catalytic mechanism of SaTatD. Among the key residues composing the active site, the acidic residues Glu92 and Glu202 had a critical impact on catalysis by SaTatD. Furthermore, based on the binding mode of the two phosphates and structural insights, a putative DNA-binding mode of SaTatD was proposed using in silico docking. Overall, these findings may serve as a good basis for understanding the relationship between the structure and function of TatD proteins from Gram-positive bacteria and may provide critical insights into the DNA-binding mode of SaTatD.

When songwriter Oscar Hammerstein penned the lyrics “fish gotta swim, birds gotta fly,” he clearly did not have the ratites in mind. Large flightless birds, the ratites include ostriches, emus, rheas and cassowaries, along with kiwis and several extinct species.

The post DNA evidence is rearranging the branches of the avian family tree appeared first on Smithsonian Insider.

The first DNA barcoding survey of crustaceans living on samples of dead coral taken from the Indian, Pacific and Caribbean oceans suggests that the diversity of organisms living on the world’s coral reefs—one of the most endangered habitats on Earth—is seriously underestimated.

The post New DNA study suggests coral reef biodiversity is seriously underestimated appeared first on Smithsonian Insider.

Both investigations were carried out through DNA analysis of fish tissue performed in a laboratory using a U.S. Food and Drug Administration protocol that originated largely at the Smithsonian’s National Museum of Natural History. DNA from the fish in question was identified by matching it against a database of DNA fish barcodes that again, has its origins at the Smithsonian.

The post Smithsonian research with DNA barcoding is making seafood substitution easier to catch appeared first on Smithsonian Insider.

The western long-beaked echidna, one of the world's five egg-laying species of mammal, became extinct in Australia thousands of years ago…or did it?

The post Scientists discover long-beaked echidna may not be a thing of the past in Australia appeared first on Smithsonian Insider.

A new DNA study of ancient corn kernels and cobs from archaeological sites across North America has settled a long debate as to exactly where […]

The post Corn entered Southwest U.S. first along highland route, DNA shows appeared first on Smithsonian Insider.

DNA analysis is proving to be a valuable tool for scientists trying to gauge the environmental impact that invasive blue catfish (Ictalurus furcatus) and flathead […]

The post DNA solves puzzle of catfish dinners appeared first on Smithsonian Insider.

Smothered in tartar sauce and cheese it’s difficult to know just what species of fish lurks beneath the breaded surface of a fast-food fish sandwich. […]

The post Smithsonian scientist creating DNA database to track Caribbean conch and lobster appeared first on Smithsonian Insider.

It is rare but not entirely uncommon to see a manatee swimming in the Atlantic waters of Maryland and Virginia. This one was dead, however, […]

The post In face of mass extinctions, Smithsonian’s Global Genome Initiative quietly saves world’s DNA appeared first on Smithsonian Insider.

A bite mark on a homicide victim’s skin is not an unusual discovery. A criminal’s teeth marks are also occasionally found on food at a […]

The post Crime bite: DNA on half-eaten food may someday send crooks to jail appeared first on Smithsonian Insider.

A bat specimen collected in France at the end of World War I, since housed in the collections of the Smithsonian’s National Museum of Natural […]

The post DNA on 100-year-old bat from France may help fight deadly fungus in North America appeared first on Smithsonian Insider.

Imke K. Mandemaker, Di Zhou, Serena T. Bruens, Dick H. Dekkers, Pernette J. Verschure, Raghu R. Edupuganti, Eran Meshorer, Jeroen A. Demmers, and Jurgen A. Marteijn

Many chromatin remodeling and modifying proteins are involved in the DNA damage response by stimulating repair or inducing DNA damage signaling. Interestingly, here we identified that down regulation of the H1-interacting protein SET results in increased resistance to a wide variety of DNA damaging agents. We found that this increased resistance is not the result of an inhibitory effect of SET on DNA repair, but rather the consequence of a suppressed apoptotic response to DNA damage. We further provide evidence that the histone chaperone SET is responsible for the eviction of H1 from chromatin. Knock down of H1 in SET-depleted cells resulted in re-sensitization of cells to DNA damage, suggesting that the increased DNA damage resistance in SET-depleted cells is the result of enhanced retention of H1 on chromatin. Finally, clonogenic survival assays show that SET and p53 are epistatic in attenuating DNA damage-induced cell death. Altogether, our data show a role for SET in the DNA damage response as a regulator of cell survival following genotoxic stress.

Isabella Guardamagna, Elisabetta Bassi, Monica Savio, Paola Perucca, Ornella Cazzalini, Ennio Prosperi, and Lucia A. Stivala

Assessing DNA repair is an important endpoint to study the DNA damage response for investigating the biochemical mechanisms of this process and the efficacy of chemotherapy, which often uses DNA damaging compounds. Numerous in vitro methods to biochemically characterize DNA repair mechanisms have been developed so far. However, they show some limitations mainly due to the lack of chromatin organization. Here we describe a functional cell-free system to study DNA repair synthesis in vitro, using G1-phase nuclei isolated from human cells treated with different genotoxic agents. Upon incubation in the correspondent damage-activated cytosolic extracts, containing biotin-16-dUTP, nuclei are able to initiate DNA repair synthesis. The use of specific DNA synthesis inhibitors markedly decreased biotinylated dUTP incorporation, indicating the specificity of the repair response. Exogenously added human recombinant PCNA protein, but not the sensors of UV-DNA damage DDB2 or DDB1, stimulated UVC induced dUTP incorporation. In contrast, a DDB2^PCNA- mutant protein, unable to associate with PCNA, interfered with DNA repair synthesis. Given its responsiveness to different type of DNA lesions, this system offers an additional tool to study DNA repair mechanisms.

The humble salamander may provide evidence to support a controversial claim that North and South America were joined together much earlier than previously thought. The […]

The post Salamander DNA reveals evidence of older land connection between Central and South America appeared first on Smithsonian Insider.

When Smithsonian Conservation Biology Institute conservation biologist Jessica Deichmann joined a project to determine how the construction of a road in Gabon’s Moukalaba-Doudou National Park […]

The post DNA untangles Gabon’s complex web of frog species appeared first on Smithsonian Insider.

Researchers at the Smithsonian's National Zoo's new genetics lab use animal DNA to diagnose new diseases, discover new species help in conservation efforts and solve mysteries.

The post Scientists at a new National Zoo laboratory use DNA to diagnose illnesses and discover new species of animals appeared first on Smithsonian Insider.

Recent analysis using DNA sequencing of ancient Roman pills found aboard a ship that sank in Italy’s Gulf of Baratti between 140 -120 B.C, has revealed that the medicines consist of material from simple garden plants, namely carrot, radish, parsley, celery, wild onion and cabbage.

The post DNA sequencing reveals simple vegetables in ancient Roman medicines appeared first on Smithsonian Insider.