As the COVID-19 pandemic continues to spread, thousands of scientists around the globe have changed research direction to understand better how the virus works and to find out how it may be tackled. The number of manuscripts on preprint servers is soaring and peer-reviewed publications using MS-based proteomics are beginning to emerge. To facilitate proteomic research on SARS-CoV-2, the virus that causes COVID-19, this report presents deep-scale proteomes (10,000 proteins; >130,000 peptides) of common cell line models, notably Vero E6, Calu-3, Caco-2, and ACE2-A549 that characterize their protein expression profiles including viral entry factors such as ACE2 or TMPRSS2. Using the 9 kDa protein SRP9 and the breast cancer oncogene BRCA1 as examples, we show how the proteome expression data can be used to refine the annotation of protein-coding regions of the African green monkey and the Vero cell line genomes. Monitoring changes of the proteome on viral infection revealed widespread expression changes including transcriptional regulators, protease inhibitors, and proteins involved in innate immunity. Based on a library of 98 stable-isotope labeled synthetic peptides representing 11 SARS-CoV-2 proteins, we developed PRM (parallel reaction monitoring) assays for nano-flow and micro-flow LC–MS/MS. We assessed the merits of these PRM assays using supernatants of virus-infected Vero E6 cells and challenged the assays by analyzing two diagnostic cohorts of 24 (+30) SARS-CoV-2 positive and 28 (+9) negative cases. In light of the results obtained and including recent publications or manuscripts on preprint servers, we critically discuss the merits of MS-based proteomics for SARS-CoV-2 research and testing.

SA's financial regulator is investigating allegations that agents of online brokerage Banxso, whose licence has been suspended, contacted clients claiming it has been "cleared".

Disguised in different roles–from rebellious teenagers to homeless people to prostitutes and pimps–community members responsible for the onshore segment of The Agency experience act out the types of least reached people in society.

Recent reports of misleading comments in response to consumer questions prompt bulletin re-issuance The Delaware Department of Insurance has been made aware of recent misrepresentations related to the basis for premium changes in consumer-facing communications. In response, the department is reminding agents, producers, and insurers of their obligations to provide full explanations of rate changes […]

Exogenous substances, including drugs and chemicals, can transfer into human seminal fluid and influence male fertility and reproduction. In addition, substances relevant in the context of sports drug testing programs, can be transferred into the urine of a female athlete (after unprotected sexual intercourse) and trigger a so-called adverse analytical finding. Here, the question arises as to whether it is possible to distinguish analytically between intentional doping offenses and unintentional contamination of urine by seminal fluid. To this end, 480 seminal fluids from nonathletes were analyzed to identify concentration ranges and metabolite profiles of therapeutic drugs that are also classified as doping agents. Therefore, a screening procedure was developed using liquid chromatography connected to a triple quadrupole mass spectrometer, and suspect samples (i.e., samples indicating the presence of relevant compounds) were further subjected to liquid chromatography-high-resolution accurate mass (tandem) mass spectrometry. The screening method yielded 90 findings (including aromatase inhibitors, selective estrogen receptor modulators, diuretics, stimulants, glucocorticoids, beta-blockers, antidepressants, and the nonapproved proliferator-activated receptor delta agonist GW1516) in a total of 81 samples, with 91% of these suspected cases being verified by the confirmation method. In addition to the intact drug, phase-I and -II metabolites were also occasionally observed in the seminal fluid. This study demonstrated that various drugs including those categorized as doping agents partition into seminal fluid. Monitoring substances and metabolites may contribute to a better understanding of the distribution and metabolism of exogenous substances in seminal fluid that may be responsible for the impairment of male fertility.

There is a growing interest in the use of medicinal plants to treat a variety of diseases, and one of the most commonly used medicinal plants globally is Cannabis sativa. The two most abundant cannabinoids (⁹-tetrahydrocannabinol and cannabidiol) have been governmentally approved to treat selected medical conditions; however, the plant produces over 100 cannabinoids, including cannabichromene (CBC). Although the cannabinoids share a common precursor molecule, cannabigerol, they are structurally and pharmacologically unique. These differences may engender differing therapeutic potentials. In this review, we will examine what is currently known about CBC with regards to pharmacodynamics, pharmacokinetics, and receptor profile. We will also discuss the therapeutic areas that have been examined for this cannabinoid, notably antinociceptive, antibacterial, and anti-seizure activities. Finally, we will discuss areas where new research is needed and potential novel medicinal applications for CBC.

²²⁶Ac (t_1/2 = 29.37 h) has been proposed as a theranostic radioisotope leveraging both its diagnostic -emissions and therapeutic α-emissions. ²²⁶Ac emits 158 and 230 keV -photons ideal for quantitative SPECT imaging and acts as an in vivo generator of 4 high-energy α-particles. Because of these nuclear decay properties, ²²⁶Ac has potential to act as a standalone theranostic isotope. In this proof-of-concept study, we evaluated a preclinical ²²⁶Ac-radiopharmaceutical for its theranostic efficacy and present the first ²²⁶Ac-targeted α-therapy study. Methods: ²²⁶Ac was produced at TRIUMF and labeled with the chelator-peptide bioconjugate crown-TATE. [²²⁶Ac]Ac-crown-TATE was selected to target neuroendocrine tumors in male NRG mice bearing AR42J tumor xenografts for SPECT imaging, biodistribution, and therapy studies. A preclinical SPECT/CT scanner acquired quantitative images reconstructed from both the 158 and the 230 keV emissions. Mice in the biodistribution study were euthanized at 1, 3, 5, 24, and 48 h after injection, and internal radiation dosimetry was derived for the tumor and organs of interest to establish appropriate therapeutic activity levels. Mice in the therapy study were administered 125, 250, or 375 kBq treatments and were monitored for tumor size and body condition. Results: We present quantitative SPECT images of the in vivo biodistribution of [²²⁶Ac]Ac-crown-TATE, which showed agreement with ex vivo measurements. Biodistribution studies demonstrated high uptake (>30%IA/g at 5 h after injection) and retention in the tumor, with an estimated mean absorbed dose coefficient of 222 mGy/kBq. [²²⁶Ac]Ac-crown-TATE treatments significantly extended the median survival from 7 d in the control groups to 16, 24, and 27 d in the 125, 250, and 375 kBq treatment groups, respectively. Survival was prolonged by slowing tumor growth, and no weight loss or toxicities were observed. Conclusion: This study highlights the theranostic potential of ²²⁶Ac as a standalone therapeutic isotope in addition to its demonstrated diagnostic capabilities to assess dosimetry in matched ²²⁵Ac-radiopharmaceuticals. Future studies will investigate maximum dose and toxicity to further explore the therapeutic potential of ²²⁶Ac-radiopharmaceuticals.

Jade Ho explains what is possible for university students when they are given opportunities to learn about –and get involved with– social justice and labour issues in their own communities.

The post Students can be agents of change: Talking about activism in universities with Jade Ho appeared first on rabble.ca.

Chris develops a Yakuza game with all Pokémon. Josh is surprised by microtransactions. And Kelley get surprised by a secret mobile game.

The post RPG Cast – Episode 622: “Secret Maple Syrup Agent” appeared first on RPGamer.

Agents are being offered double the normal commission to help shift apartments throughout capital cities.

Farmers Insurance "We Know From Experience" Ad Campaign Spot

They created their own path. Will you create yours?

The internet has been buzzing with excitement over what many fans initially thought was an official Agent Venom trailer from Sony. The clip, featuring Tony Revolori as Flash Thompson transforming into Agent Venom, quickly went viral and sparked discussions about a possible new Venom movie. Fans seem to be excited about the prospect of seeing […]

The post Why Fans Think Sony’s Agent Venom Trailer Is Real appeared first on ComingSoon.net - Movie Trailers, TV & Streaming News, and More.

Highlights: Researchers at JNU have discovered a novel chemical with strong transmission-blocking activity for

medlinkZebrafish/medlink exhibits a crucial role in the fields of solid organ preservation and transplantation, especially with respect to experimental transplantation efforts.

New real estate norms have jazzed up the monotonous property market. This has encouraged salesmen and agents to sell the property by offering freebies and discounts.

The strategy is to create consumer agents, but then use those to inform how they train agents in the enterprise.

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LinkedIn, the social platform used by professionals to connect with others in their field, hunt for jobs, and develop skills, is taking the wraps off its latest effort to build artificial intelligence tools for users. Hiring Assistant is a new product designed to take on a wide array of recruitment tasks, from ingesting scrappy notes […]

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AI code assistant company Tabnine is launching a code review agent today that aims to help developers stick to their organization’s best practices and standards. It allows organizations to codify these rules either by providing the agent with documentation or by pointing it at a set of “golden code repos.” The agent will passively review […]

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AgentOps uses the Gemini API to provide cost-effective and powerful LLM-powered agent observability for enterprises.

Cambridge, MA. : National Bureau of Economic Research, 2023

The study says that GenAI use cases have mostly been limited to standalone applications

Former Secret Service Agent Jonathan Wackrow, now managing director at Teneo Risk, explains how the Service protects the President and other VIPs. Wackrow details the many steps required to ensure the safety of protectees, and describes how the Service has changed due to changing technology and lessons learned from historical events. Wackrow served in the Presidential Protection Division in Washington, DC, and managed numerous high-level security operations in the U.S. and abroad.

According to Jim Clemente, retired FBI behavioral analyst and criminal profiler, when it comes to the "nature vs. nurture" debate, "Genetics loads the gun, personality and psychology aim it, and your experiences pull the trigger..." As a criminal profiler, it was Jim's job to catch murderers, serial killers and rapists and pick up the slack where forensic evidence failed.

Former Secret Service Agent Jonathan Wackrow explains how the Service keeps counterfeit currency out of circulation. The Secret Service was installed to combat counterfeit money during the Civil War, and the Service still to this day works tirelessly to suppress counterfeits. Wackrow served in the Presidential Protection Division in Washington, DC, and managed numerous high-level security operations in the U.S. and abroad.

There are a number of myths about detecting deception. Fidgeting, looking away, touching your mouth, all of these things are commonly thought to be practices that indicate deception. Jim Clemente, former Supervisory Special Agent for the FBI, explains why that isn't always the case and how people like him can decipher what these indicators really mean.

Former FBI agent and body language expert Joe Navarro is back, this time to breaks down the non-verbal ways we communicate using facial expressions. What does it mean when we scrunch up our noses or show tension in the glabella? Joe also goes deep into chirality, and equates this concept with some of the most quizzical of human expressions. Check out Joe's book "The Dictionary of Body Language" https://www.jnforensics.com/

Retired FBI Special Agent Rhonda Glover Reese takes a look at some scenes featuring surveillance in movies and television and explains how accurate they really are.

Former FBI agent and body language expert Joe Navarro breaks down the intentional (and unintentional) non-verbals displayed by politicians. Why do some politicians point with their entire hand? What does it mean when politicians wave to a large crowd? How do Donald Trump and Joe Biden's non-verbals compare to each other? Check out Joe's book "The Dictionary of Body Language" https://www.jnforensics.com/ Books By Joe Navarro: https://www.jnforensics.com/books Joe Navarro Body Language Academy: https://jnbodylanguageacademy.com

Former FBI agent and body language expert Joe Navarro breaks down the unintentional non-verbals that are displayed by human beings around the globe. Joe explains how the limbic system functions and why we act the way we do in certain situations. Check out Joe's book "The Dictionary of Body Language" https://www.jnforensics.com/ Books By Joe Navarro: https://www.jnforensics.com/books Joe Navarro Body Language Academy: https://jnbodylanguageacademy.com

Former FBI agent and body language expert Joe Navarro breaks down how he approached interrogating during his time with the FBI. Joe sets the record straight; there is no simple way to tell if a human being is lying. When looking for deceit, Joe focuses on the subject's discomfort. He also does is best to make sure the subject feels comfortable enough to open up...a far cry from the images we see in many police procedurals. Check out Joe's book "The Dictionary of Body Language" https://www.jnforensics.com/ Books By Joe Navarro: https://www.jnforensics.com/books Joe Navarro Body Language Academy: https://jnbodylanguageacademy.com

Former FBI agent and body language expert Joe Navarro breaks down the intentional (and unintentional) non-verbals displayed during dating. What kind of signs do we show during dates that we don't even realize? Can we display our affection for someone without even explaining it verbally? Check out Joe's book "The Dictionary of Body Language" https://www.jnforensics.com/ Books By Joe Navarro: https://www.jnforensics.com/books Joe Navarro Body Language Academy: https://jnbodylanguageacademy.com

Former FBI agent and body language expert Joe Navarro answers the internet's burning questions about body language. Why is being stared at so intimidating? How do you have a good poker face? Can body language be admitted as evidence in court? How can you end a conversation politely? Joe answers all these questions and much more! Check out Joe's book "Be Exceptional" https://www.jnforensics.com/ Books By Joe Navarro: https://www.jnforensics.com/books Joe Navarro Body Language Academy: https://jnbodylanguageacademy.com