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A predicted model-aided one-step classification–multireconstruction algorithm for X-ray free-electron laser single-particle imaging

Ultrafast, high-intensity X-ray free-electron lasers can perform diffraction imaging of single protein molecules. Various algorithms have been developed to determine the orientation of each single-particle diffraction pattern and reconstruct the 3D diffraction intensity. Most of these algorithms rely on the premise that all diffraction patterns originate from identical protein molecules. However, in actual experiments, diffraction patterns from multiple different molecules may be collected simultaneously. Here, we propose a predicted model-aided one-step classification–multireconstruction algorithm that can handle mixed diffraction patterns from various molecules. The algorithm uses predicted structures of different protein molecules as templates to classify diffraction patterns based on correlation coefficients and determines orientations using a correlation maximization method. Tests on simulated data demonstrated high accuracy and efficiency in classification and reconstruction.




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Crystal structures of two new high-pressure oxynitrides with composition SnGe4N4O4, from single-crystal electron diffraction

SnGe4N4O4 was synthesized at high pressure (16 and 20 GPa) and high temperature (1200 and 1500°C) in a large-volume press. Powder X-ray diffraction experiments using synchrotron radiation indicate that the derived samples are mixtures of known and unknown phases. However, the powder X-ray diffraction patterns are not sufficient for structural characterization. Transmission electron microscopy studies reveal crystals of several hundreds of nanometres in size with different chemical composition. Among them, crystals of a previously unknown phase with stoichiometry SnGe4N4O4 were detected and investigated using automated diffraction tomography (ADT), a three-dimensional electron diffraction method. Via ADT, the crystal structure could be determined from single nanocrystals in space group P63mc, exhibiting a nolanite-type structure. This was confirmed by density functional theory calculations and atomic resolution scanning transmission electron microscopy images. In one of the syntheses runs a rhombohedral 6R polytype of SnGe4N4O4 could be found together with the nolanite-type SnGe4N4O4. The structure of this polymorph was solved as well using ADT.




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Hard X-ray single-shot spectrometer of PAL-XFEL

A hard X-ray single-shot spectrometer comprising thin, bent Si crystals has been developed for the Pohang Accelerator Laboratory X-ray Free-Electron Laser (XFEL), for detailed analysis of ultrafast 4.5–17 keV XFEL pulses with a high spectral resolution. This instrument facilitates shot-to-shot spectral structure monitoring and optimization of the operating conditions of the XFEL owing to its ability to provide comprehensive data on the spectral properties and fluctuations of self-amplified spontaneous emission, monochromatic and seeded XFEL modes.




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Enhancing the Efficiency of a Wavelength-Dispersive Spectrometer based upon a Slit-less Design Using a Single-Bounce Monocapillary

A slit-less wavelength-dispersive spectrometer design using a single-bounce monocapillary that aligns the sample on the Rowland circle, enhancing photon throughput and maintaining resolution. The compact design supports flexibility and reconfiguration in facilities without complex beamline infrastructure, significantly improving detection efficiency.




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Texture measurements on quartz single crystals to validate coordinate systems for neutron time-of-flight texture analysis

In crystallographic texture analysis, ensuring that sample directions are preserved from experiment to the resulting orientation distribution is crucial to obtain physical meaning from diffraction data. This work details a procedure to ensure instrument and sample coordinates are consistent when analyzing diffraction data with a Rietveld refinement using the texture analysis software MAUD. A quartz crystal is measured on the HIPPO diffractometer at Los Alamos National Laboratory for this purpose. The methods described here can be applied to any diffraction instrument measuring orientation distributions in polycrystalline materials.




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A workflow for single-particle structure determination via iterative phasing of rotational invariants in fluctuation X-ray scattering

Fluctuation X-ray scattering (FXS) offers a complementary approach for nano- and bioparticle imaging with an X-ray free-electron laser (XFEL), by extracting structural information from correlations in scattered XFEL pulses. Here a workflow is presented for single-particle structure determination using FXS. The workflow includes procedures for extracting the rotational invariants from FXS patterns, performing structure reconstructions via iterative phasing of the invariants, and aligning and averaging multiple reconstructions. The reconstruction pipeline is implemented in the open-source software xFrame and its functionality is demonstrated on several simulated structures.




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Coherent X-ray diffraction imaging of single particles: background impact on 3D reconstruction

Coherent diffractive imaging with X-ray free-electron lasers could enable structural studies of macromolecules at room temperature. This type of experiment could provide a means to study structural dynamics on the femtosecond timescale. However, the diffraction from a single protein is weak compared with the incoherent scattering from background sources, which negatively affects the reconstruction analysis. This work evaluates the effects of the presence of background on the analysis pipeline. Background measurements from the European X-ray Free-Electron Laser were combined with simulated diffraction patterns and treated by a standard reconstruction procedure, including orientation recovery with the expand, maximize and compress algorithm and 3D phase retrieval. Background scattering did have an adverse effect on the estimated resolution of the reconstructed density maps. Still, the reconstructions generally worked when the signal-to-background ratio was 0.6 or better, in the momentum transfer shell of the highest reconstructed resolution. The results also suggest that the signal-to-background requirement increases at higher resolution. This study gives an indication of what is possible at current setups at X-ray free-electron lasers with regards to expected background strength and establishes a target for experimental optimization of the background.




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Use of a confocal optical device for centring a diamond anvil cell in single-crystal X-ray diffraction experiments

High-pressure crystallographic data can be measured using a diamond anvil cell (DAC), which allows the sample to be viewed only along a cell vector which runs perpendicular to the diamond anvils. Although centring a sample perpendicular to this direction is straightforward, methods for centring along this direction often rely on sample focusing, measurements of the direct beam or short data collections followed by refinement of the crystal offsets. These methods may be inaccurate, difficult to apply or slow. Described here is a method based on precise measurement of the offset in this direction using a confocal optical device, whereby the cell centre is located at the mid-point of two measurements of the distance between a light source and the external faces of the diamond anvils viewed along the forward and reverse directions of the cell vector. It is shown that the method enables a DAC to be centred to within a few micrometres reproducibly and quickly.




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No Single Solution for Protecting Kids From Internet Pornography

No single approach -- technical, legal, economic, or educational -- will be sufficient to protect children from online pornography.




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Single Breakthrough Discovery for Citrus Greening Disease in Florida Unlikely, Says New Report

A single breakthrough discovery for managing citrus greening in Florida in the future is unlikely, says a new report by the National Academies of Sciences, Engineering, and Medicine.




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Organohalogen Flame Retardants Used in Consumer Products Cannot Be Assessed for Hazards as a Single Class, But Can Be Assessed in Subclasses, Says New Report

A new report from the National Academies of Sciences, Engineering, and Medicine offers guidance to the Consumer Product Safety Commission (CPSC) on how to conduct a hazard assessment of nonpolymeric, additive organohalogen flame retardants (OFRs), which are used in some consumer products.




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Heritable Genome Editing Not Yet Ready to Be Tried Safely and Effectively in Humans - Initial Clinical Uses, If Permitted, Should Be Limited to Serious Single-Gene Diseases

Human embryos whose genomes have been edited should not be used to create a pregnancy until it is established that precise genomic changes can be made reliably without introducing undesired changes — a criterion that has not yet been met by any genome editing technology, says a new report by an international commission of the U.S. National Academy of Medicine, U.S. National Academy of Sciences, and the U.K.’s Royal Society.




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Case Study - Telepresence enables SASOL to operate as a single global community

SASOL has adopted telepresence for collaboration between far-flung executives in Europe, North America and Africa. Running over the BT IP Connect global network it also enables federation with customers and suppliers. The solution’s earned Alec’s team a special recognition award from the CEO for bringing the company’s One SASOL philosophy and vision vibrantly to life




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Industry urges TRAI to adopt single technology for digital radio roll-out

The association suggested that All India Radio separately assess DRM and HD Radio for MW and SW bands, which fall under its exclusive remit. It also recommended that analogue transmission should not be interrupted until digital receivers are adopted by more than 80% of the population to ensure accessibility.




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X-ray inspection system for single pack products, from Mettler-Toledo

Mettler Toledo's X34C delivers a unique X-ray solution to the market: cost-effective inspection of individual packs and bars, immediately after flow-wrapping or pack sealing.




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Valrhona vegan-certified Grand Cru single origin 'milk' chocolate, from the heart of Madagascar

Valrhona marks a milestone in chocolate-making with AMATIKA 46%, the first vegan Grand Cru chocolate available for both pastry chefs and home gourmets.




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Arcadia Biosciences breaks into breakfast category with Pancake & Waffle Mixes, Single-Serve Quikcakes

Arcadia Biosciences, Inc. announced it is expanding its better-for-you, fiber-forward brand, GoodWheat, with the upcoming launch of GoodWheat Quikcakes and GoodWheat Pancake & Waffle Mixes.




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Be Happy Snacks launches single-serve bags

The popcorn brand created by the "First Family of TikTok" continues the summer fun with new single-serve size and exciting theme park giveaway.




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Wonder Monday launches single-serve, retail-ready cakes

Wonder Monday has reimagined cheesecake with just 2g sugar, 3g net carbs, 10g clean protein, and nothing artificial. 




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Rich Products, Funfetti debut Cheesecake Single Slice

The 3.25-ounce Funfetti Cheesecake Slice comes 24 per case, with a shelf life of 365 days frozen and five days refrigerated.




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Wild About Bread launches single-pack flavors at Kroger stores in Dallas

Wild About Bread Home Baked Bread Made Simple is now even more easy and fun with the launch of new Single Pack Flavors available at Kroger stores in Dallas/Fort Worth Metroplex in Texas. 




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Kraft Singles slice into dessert space with Little Pie Company collaboration

The Kraft Singles Apple Pie tops a single-serve pie with a piece of American cheese.




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Entenmann’s refreshes Baker’s Delights single-serve treats branding

The sweet baked goods brand has renamed its Minis line with a new name and packaging.




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Hebenstreit single-screw extruders

Hebenstreit GmbH is now offering manufacturers new technology designed for the manufacture of extruded snacks: the SE 70 and SE 110 single-screw cooking extruders.




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3 reasons why a single-source of truth is vital to MEP contractors

It’s no secret that MEP contractors deliver massive value on every project out there. In 2019 alone, MEP contractors generated 46.3% ($70 billion) of total revenue delivered by the ENR Top 600 Specialty Contractors.




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Single coil solution

Some renewable energy heat sources require large thermal storage tanks. Examples include systems’ biomass boilers and solar thermal collectors.




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Ranpak’s Cut’it!™ EVO Multi-Lid allows four unique branded lids on a single machine

Packaging solution automatically shortens cartons to match their highest point of filling and then glues a lid securely in place, reducing material usage and costs.




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Blackline Safety unveils new and improved features for G6 Single-Gas Detector

G6 will be on display at a global marketing roadshow at both the National Safety Council (NSC) Safety Congress & Expo in New Orleans from October 23-35, and A+A in Dusseldorf, Germany from October 24-27.




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Busker Irish Whiskey’s Single Collection unveils new bottle designs

The Busker Irish Whiskey unveiled its new premium bottle designs for its Single Collection: Single Malt, Single Grain, and Single Pot Still. These new designs now are available and highlight the quality of The Busker's highly-awarded whiskey single expressions, the company says. 




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Hughes & Coleman Injury Lawyers Secures $400,000 Settlement for Single Mother Involved In Kentucky Auto Accident

$400,000 settlement is more than 4X the initial insurance company offer




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Marquis Who's Who Honors Nick Singleton, MBA, for Expertise in Data Engineering

Nick Singleton, MBA, is a seasoned leadership and business strategy expert in his leading role at Integra Connect LLC




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Voices Of Service to Release New Single 'Hope"

That feeling is what we wanted to capture in this song. That little spark of hope and inspiration to help turn your day, week and year around. said Producer David "DQ" Quinones




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South Fork releases new fiber optic based single-use UV absorbance system

Kemtrak DCP007 is designed to accurately measure UV absorbance of fluids in filtration and separation systems




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FreeDatingSitesUK Unveils Best Dating Sites Reviews for Over 50 Singles

The comprehensive new review offers an honest look at the top 9 dating sites for singles over 50 in the UK.




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SeniorMatch Reveals the Top 10 Cities for Casual Dates for Senior Singles in Their 50s

SeniorMatch Unveils the Top 10 Cities Redefining Senior Dating: Where Casual Connections Flourish for Singles in Their 50s




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MIGOS DOG LAUNCHES SINGLE-INGREDIENT JERKY TREATS

Migos Jerky is the newest offering to complement the Malibu-based brand's fresh dog food and nutritional supplement




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Harold Singleton III Celebrated for Dedication to the Financial Services Industry

Harold Singleton III channels years of expertise into board service with Hershey Trust Company and Fidelity Rutland Square Trust II at Fidelity Investments




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Wadena Sets The Stage Ablaze With Her Captivating New Single "Smoke-n-Chill" Hempstead, NY (August 30, 2024)

Wadena, a talented singer and songwriter based in New York, is known for her captivating voice, energetic performances, and genre-bending style that blends R&B, Hip-hop, Pop, and Trap Music.




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Abby Volta's new single is out this Halloween

Goth Rock Singer Abby Volta is releasing her single "Rabbit Catcher" October 31st on all major streaming platforms.




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Pop Star Jasmine Ortiz Shines The Spotlight On Colombia With Her Next Single "En La Disco" and Tour

"En La Disco" Produced by Multi-Platinum Producer SOG, the most sought-after Producer in Colombia!




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Michael Go releases limited edition singles through web3 music marketplace Serenade and Berklee Valencia's Disrupción Records

Artist profits donated to Grand'Anse, Haiti-based sustainable agriculture project led by the Julia&Jade - KOSÃHA Foundation




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Long lost 1970s Video of legendary NY York Rock Band, That Hideous Strength featuring Roy Wagner, discovered performing on The Danny Conrad 1975 TV Show & Posted on You Tube. Studio single sold online

Imagine Motorhead Meets the Ramones & You have the original "They Call Me Energy" while the whimsical original "A Night at The Space Opera" sounds a bit like Pink Floyd with Syd Barrett meets the early Who. Now available at Amazon & other fine sites




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Country Songstress Bekka Dowland Drops New Single "Malibu" Out Now!

Stream "Malibu" by Bekka Dowland and get a sneak peek of the "Malibu" music video filmed in Cape Coral and Fort Meyers, Florida!




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Karlie Redd Drops Explosive New Single "Bumper Heavy" Featuring Dance Hall Icon Beenie Man and Songstress Destra: A New Caribbean Anthem!

Rising Music Powerhouse is back with another reggae hit!




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'Well Played' Drops Debut Single 'Bitter' - A Melodic Masterpiece!

Paying homage to the golden age of Pop Punk, Northern California's newest band comes out swinging with their debut single.




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Prominent Social Content Creator and Rising American Reggae Talent Releases First Single

"Turkoise" showcases the vocal prowess of singer/songwriter Alyxandra while expressing her deep admiration for Turks and Caicos, where she calls home.




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Pop Sensation AnDy Darling Teams up with BDD Foundation to Raise Awareness About Body Dysmorphic Disorder in Her New Single "skinny"

New Meaningful Partnership Between the Singer-Songwriter and BDD Foundation




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Mesmerizing Follow-Up Single Unveiled by American Reggae and Pop Sensation

Alyxandra's new song "Float" takes listeners on a fun trip with plenty of euphoric feelings and good times.




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Chicago Music Award Nominee Dantès Alexander, The Prince Regent of EDM, reveals "They Won't Go When I Go," his latest single & album. Featuring captivating remixes by DJ Aaron Chase & more

Chicago Music Award Nominee Dantès Alexander, The Prince Regent of EDM, unveils "They Won't Go When I Go," his latest single & album. With captivating remixes by Aaron Chase & more, it promises an electrifying journey for fans worldwide.




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SCCMPod-442 Continuous Prediction of Mortality in the PICU: A Recurrent Neural Network Model in a Single-Center Dataset

As a proof of concept, a recurrent neural network (RNN) model was developed using electronic medical record (EMR) data capable of continuously assessing a child's risk of mortality throughout an ICU stay as a proxy measure of illness severity.