Location: Electronic Resource- 

Vernon’s Song - a Christmas anthem for the homeless.

Bobby Springsteen is seeking an EDM Artist/DJ that already has a completed single. Please submit your best track(s) for consideration and state if you are a writer or producer.

Dreamwire is a multi-media company focusing on management, artist development, production, and publishing.

Compensation: Co-publishing and/or Record deal with Dreamwire Ent. - $1,000+

- Bobby Springsteen / Dreamwire Entertainment

French Horn Rebellion makes music that is an irresistible fusion of funky electronic pop and retro flair, where the grooves are as infectious as the melodies are catchy. Hailing from…

a comic about really liking one song

Special guest Michael Simmons joins the show. Topics include the release of iOS 14, widgets and home screen customization, pricing models for indie apps in the App Store era, and, of course, flying robot cameras.

Celebrated jazz Hammond organist Vel Lewis is set to release his highly anticipated new single, "I Paid The Price," a soulful and introspective piece showcasing his signature mastery of jazz, soul, and blues. The single will be available on all major streaming platforms on November 15, 2024. Lewis, known for his unmistakable touch on the Hammond organ, has crafted "I Paid The Price" to reflect his deep connection to life’s lessons, love, and the resilience that comes from overcoming challenges...

This is just a quick little snippet to display a word count on the single post page in WordPress. Since it requires being placed within the loop, you can actually…

The post Add Word Count to Single Posts in WordPress appeared first on bavotasan.com.

Gypsy Pistoleros just released a new video and single called 'Like Tears In The Rain' from their forthcoming album, 'Duende A Go Go Loco!'. Gypsy Lee Pistolero tells us about the new song to celebrate

Divine Grave just released the new single 'Grief' and to celebrate we asked the mysterious metalcore act to tell us about the track

They've got plans for a new record in 2025, too.

"The communities I identify with still struggle with the aftermath of historical events."

The New York City unit returns for the first time since 2000!

Bermuda Open Singles Snooker Second Round action continued, with Peter Bromby defeating Gary Bascombe 2-0 at Sandys Boat Club. Bromby won the first game 62–21, he would win the second game 85–26, Bromby now meets Paul Fedden in the quarter-final. At the Warwick Workmen’s Club, Todd Dziak defeated Winslow Smith 2 – 1, Smith took a […]

Peter Bromby edged Paul Fedden 2-1 at Spanish Point Boat Club. Fedden was first to score when he defeated Bromby 61-44 in the 1st game, but Bromby stormed back in the 2nd when he defeated Fedden 54-44. It was Bromby who prevailed in the 3rd game when he sealed the victory, taking it 57-51. Aaron […]

Action in Bermuda snooker open singles and open doubles took place. Jacal Washington advanced in his open singles 2nd round match defeating Chris Dakin 2 – 0. Washington won the 1st game 37-36, he then won the 2nd game 48-26. Washington will face Todd Dziak in the quarter-finals. Steven Bremar advanced to the open singles […]

The Snooker Season came to an end with the playing of the Tony DeSilva Memorial Cup & the Bermuda Open Singles Finals. Peter Bromby defeated Steven Bremar 4 – 3 to claim the Tony DeSilva Memorial Cup. Bromby stormed into an early lead when he took the 1st game 64-16, Bremar leveled the scores at […]

Arizona State University men’s tennis player Daniel Phillips opened the US College Cup Invitational with a victory in singles competition Wednesday. He defeated Tommy Czaplinski of Rice University 6-1, 7-6. Phillips and doubles partner Mikey Anderson lost their match 6-2 to the Rice duo of Eduardo Morais and Yair Sarouk. Related Stories Photos: Manders & […]

Trey Mallory and his UNC Wilmington [UNCW] men’s tennis teammates opened their campaign at the UNCW team invitational with a convincing 7 – 2 victory over Coastal Carolina. Mallory contributed significantly to UNC Wilmington’s success, securing victories in both doubles and singles competition. He partnered with Oscar Baumgartner in doubles play, defeating the Coastal Carolina […]

Bermudian author Ravoshia Whaley has simultaneously released her debut book, The Mastermind: Introducing Lady Einstein, and companion music single. Ms Whaley, whose father is Bermudian, was born in Merrillville, Indiana, but was raised in Bermuda and Texas. A synopsis of the science fiction book on Amazon reads: “Samara Gumbs aka ‘The Mastermind/Lady Einstein’ is a […]

[Written by Stephen Wright] Bermudian recording artist Charmarie Woolridge-Virgin has released her new single as she looks to “inspire and touch people” through gospel music. Ms Woolridge-Virgin, the daughter of renowned Bermudian musician and producer John Woolridge, released her single, Been There, on all streaming platforms last week. The 38-year-old, who performs under the name […]

[Updated] A truck crashed on Middle Road in Devonshire this morning. A police spokesperson said, “Police officers are currently at the scene of a reported single vehicle collision in Devonshire. Details remain limited at this time. However, it is understood that a truck overturned along Middle Road, Devonshire, in the vicinity of Saltus Grammar [Lower […]

[Updated] Police responded to a single vehicle collision on North Shore Road in Hamilton parish this morning. A police spokesperson said, “Around 8:55am Monday, 30th September 2024, police officers responded to a reported single vehicle collision on North Shore Road in Hamilton parish. “Details remain limited at this time. “However, it is understood that a woman […]

Abstract: The walls of Tokamaks are heavily eroded by plasma which suggests that a mysterious force pushes the plasma to the wall. We have theoretically discovered this force and named it extra-force. This force appears only on single charges but not on current carrying wire, which is why the Lorentz force law does not contain...

All the tech from the last <i>two</i> months.
( Photos: 33, Comments: 35 )

7-minute video shows how you can a single line of text from either a .txt file or a database, for example, displaying name of duty manager. The ability to display database information is available in the Corporate and Media Wall editions of Repeat Signage digital signage software. Ideal for displaying company information on any screen anywhere.

Repeat Signage software has a number of free tools including the Batch PDF splitter. This shows you how to add your PDF to a folder and create an empty output folder. Then click run and in seconds, individual PDF pages appear in your output folder, leaving the original PDF as it was.

velocityconf: Free webcast from our friends at @citrix 5/29 http://t.co/IOeY4U0wUP Learn to consolidate 40 load balancers and ADCs into single platform

Financial Realities for Single Female Home Buyers

Dynamic singer Tosh Alexander has been lighting up the music scene with her latest track, ' My Ting Different', a thrilling collaboration with American rapper and songwriter Lady London. The song fuses R...

Lipoprotein (a) [Lp(a)] is a risk factor for CVD and a target of therapy, but Lp(a) measurements are not globally standardized. Commercially available assays generally use polyclonal antibodies that detect multiple sites within the kringle (K)IV₂ repeat region of Lp(a) and may lead to inaccurate assessments of plasma levels. With increasing awareness of Lp(a) as a cardiovascular risk factor and the active clinical development of new potential therapeutic approaches, the broad availability of reagents capable of providing isoform independence of Lp(a) measurements is paramount. To address this issue, we generated a murine monoclonal antibody that binds to only one site on apo(a). A BALB/C mouse was immunized with a truncated version of apo(a) that contained eight total KIV repeats, including only one copy of KIV₂. We generated hybridomas, screened them, and successfully produced a KIV₂-independent monoclonal antibody, named LPA-KIV9. Using a variety of truncated apo(a) constructs to map its binding site, we found that LPA-KIV9 binds to KIV₉ without binding to plasminogen. Fine peptide mapping revealed that LPA-KIV9 bound to the sequence ⁴⁰⁷⁶LETPTVV⁴⁰⁸² on KIV₉. In conclusion, the generation of monoclonal antibody LPA-KIV9 may be a useful reagent in basic research studies and in the clinical application of Lp(a) measurements.

MS-based proteome profiling has become increasingly comprehensive and quantitative, yet a persistent shortcoming has been the relatively large samples required to achieve an in-depth measurement. Such bulk samples, typically comprising thousands of cells or more, provide a population average and obscure important cellular heterogeneity. Single-cell proteomics capabilities have the potential to transform biomedical research and enable understanding of biological systems with a new level of granularity. Recent advances in sample processing, separations and MS instrumentation now make it possible to quantify >1000 proteins from individual mammalian cells, a level of coverage that required an input of thousands of cells just a few years ago. This review discusses important factors and parameters that should be optimized across the workflow for single-cell and other low-input measurements. It also highlights recent developments that have advanced the field and opportunities for further development.

Antibodies play essential roles in both diagnostics and therapeutics. Epitope mapping is essential to understand how an antibody works and to protect intellectual property. Given the millions of antibodies for which epitope information is lacking, there is a need for high-throughput epitope mapping. To address this, we developed a strategy, Antibody binding epitope Mapping (AbMap), by combining a phage displayed peptide library with next generation sequencing. Using AbMap, profiles of the peptides bound by 202 antibodies were determined in a single test, and linear epitopes were identified for >50% of the antibodies. Using spike protein (S1 and S2)-enriched antibodies from the convalescent serum of one COVID-19 patient as the input, both linear and conformational epitopes of spike protein specific antibodies were identified. We defined peptide-binding profile of an antibody as the Binding Capacity (BiC). Conceptually, the BiC could serve as a systematic and functional descriptor of any antibody. Requiring at least one order of magnitude less time and money to map linear epitopes than traditional technologies, AbMap allows for high-throughput epitope mapping and creates many possibilities.

This analysis aimed to identify clinical factors associated with positivity on repeat ⁶⁸Ga-PSMA-11 PET/CT after a negative scan in patients with recurrent prostate cancer (PCa) under observation. Methods: This single-center, retrospective analysis included patients who underwent at least 2 ⁶⁸Ga-PSMA-11 PET/CT scans (PET1 and PET2) at UCLA between October 2016 and June 2021 for recurrent PCa with negative PET1 and no PCa-related treatments between the 2 scans. Using Prostate Cancer Molecular Imaging Standardized Evaluation criteria to define negative and positive scans, the final cohort was divided into PET2-negative (PET2-Neg) and PET2-positive (PET2-Pos). The same PET1 was used twice in the more than 2 PET cases with inclusion criteria fulfilled. Patient characteristics and clinical parameters were compared between the 2 cohorts using Mann–Whitney U test and Fisher exact test. Areas under the curve (AUCs) of the receiver operating characteristic and the Youden index were computed to determine the discrimination ability of statistically significant factors and specific cut points that maximized sensitivity and specificity, respectively. Results: The final analysis included 83 sets of 2 PET/CT scans from 70 patients. Thirty-nine of 83 (47%) sets were PET2-Neg, and 44 of 83 (53%) sets were PET2-Pos. Prostate-specific antigen (PSA) increased from PET1 to PET2 for all 83 (100%) sets of scans. Median PSA at PET1 was 0.4 ng/mL (interquartile range, 0.2–1.0) and at PET2 was 1.6 ng/mL (interquartile range, 0.9–3.8). We found higher serum PSA at PET2 (median, 1.8 vs. 1.1 ng/mL; P = 0.015), absolute PSA difference (median, 1.4 vs. 0.7 ng/mL; P = 0.006), percentage of PSA change (median, +270.4% vs. +150.0%: P = 0.031), and median PSA velocity (0.044 vs. 0.017 ng/mL/wk, P = 0.002) and shorter PSA doubling time (DT; median, 5.1 vs. 8.3 mo; P = 0.006) in the PET2-Pos cohort than in the PET2-Neg cohort. Receiver operating characteristic curves showed cutoffs for PSA at PET2 of 4.80 ng/mL (sensitivity, 34%; specificity, 92%; AUC, 0.66), absolute PSA difference of 0.95 ng/mL (sensitivity, 62%; specificity, 71%; AUC, 0.68), percentage of PSA change of a positive 289.50% (sensitivity, 48%; specificity, 82%; AUC, 0.64), PSA velocity of 0.033 ng/mL/wk (sensitivity, 57%; specificity, 80%; AUC, 0.70), and PSA DT of 7.91 mo (sensitivity, 71%; specificity, 62%; AUC, 0.67). Conclusion: Patients with recurrent PCa under observation after a negative ⁶⁸Ga-PSMA-11 PET/CT scan with markedly elevated serum PSA levels and shorter PSA DT are more likely to have positive findings on repeat ⁶⁸Ga-PSMA-11 PET/CT.

Creating transgenic mosquitoes allows for mechanistic studies of basic mosquito biology and the development of novel vector control strategies. CRISPR–Cas9 gene editing has revolutionized gene editing, including in mosquitoes. This protocol details part of the gene editing process of Aedes aegypti mosquitoes via CRISPR–Cas9, through testing and validating single-guide RNAs (sgRNAs). Gene editing activity varies depending on the sequence of sgRNAs used, so validation of sgRNA activity should be done before large-scale generation of mutants or transgenics. sgRNA is designed using online tools and synthesized in <1 h. Once mutants or transgenics are generated via embryo microinjection, sgRNA activity is validated by quick genotyping polymerase chain reaction (PCR) and DNA sequencing.

In insects, gustatory neurons sense chemicals upon contact and directly inform many behaviors critical for survival and reproduction, including biting, feeding, mating, and egg laying. However, the taste sensory system is underexplored in many anthropophilic disease vectors such as mosquitoes, which acquire and transmit human pathogens during blood feeding from human hosts. This results in a big gap in vector biology—the study of organisms that spread disease by transmitting pathogens—because insect vectors closely interact with humans while selecting suitable individuals and appropriate bite sites for blood meals. Human sweat and skin-associated chemistries are rich in nonvolatile compounds that can be sensed by the mosquito's taste system when she lands on the skin. Taste sensory units, called sensilla, are distributed in many organs across the mosquito body, including the mouthparts, legs, and ovipositors (female-specific structures used to lay eggs). Each sensillum is innervated by as many as five taste neurons, which allow detection and discrimination between various tastants such as water, sugars, salts, amino acids, and plant-derived compounds that taste bitter to humans. Single-sensillum recordings provide a robust way to survey taste responsiveness of individual sensilla to various diagnostic and ecologically relevant chemicals. Such analyses are of immense value for understanding links between mosquito taste responses and behaviors to specific chemical cues and can provide insights into why mosquitoes prefer certain hosts. The results can also aid development of strategies to disrupt close-range mosquito–human interactions to control disease transmission. Here we describe a protocol that is curated for electrophysiological recordings from taste sensilla in mosquitoes and sure to yield exciting results for the field.

Canada's Roman Sadovsky is in third place after the men's short program with 84.59 points, meanwhile Canadian ice dancing duo Laurence Fournier Beaudry and Nikolaj Soerensen.

Kyle Singler's videos have left the NBA fraternity concerned with many of his former teammates offering support.

HONG KONG — Merchants and consumers alike found the Singles' Day shopping festival Monday less shiny than in years past as e-commerce firms look abroad for growth. The annual event named by the numeric form of its Nov. 11 date was started by e-commerce platform Alibaba, which offered attractive discounts to entice shoppers to spend big. The extravaganza has since expanded to other platforms like JD.com and Pinduoduo in China as well as abroad. While Singles’ Day was previously a one-day event, shopping platforms in China now kickstart the festival weeks ahead to drum up sales volume. The festival has also traditionally been regarded as a barometer of consumer sentiment. But amid China’s lagging domestic economy, dragged down by a real estate crisis and deflationary pressures, consumers no longer go all out on purchases during the shopping extravaganza. “I only spent a few hundred yuan on daily necessities,” said Wang Haihua, who owns a fitness center in Beijing. Wang said that the prices offered on e-commerce platforms during Singles’ Day are not necessarily cheaper than usual. “They’re all tricks and we’ve seen through it over the years,” she said. Zhang Jiewei, a 34-year-old who runs a barber shop in Xi’an city, echoed Wang’s sentiments, saying that he no longer trust Singles’ Day promotions as some merchants tend to raise the usual price of a product before offering a discount, giving consumers the illusion they are getting a deal. “I used to buy a lot two or three years ago and I even purchased a mobile phone (during Singles’ Day),” he said. “I stopped doing that following the pandemic because of less income. I am not going to buy anything this year,” Zhang added. Some experts say that Beijing’s recent stimulus measures have had little impact to boost consumer confidence. “People are not interested in spending and are cutting back on big-ticket items,” said Shaun Rein, founder and managing director of China Market Research Group in Shanghai. “Since October 2022, the weak economy means that everything has been on discount year-round, 11.11 is not going to bring in more discounts that the month before.” Rein said he expects low growth for the Singles’ Day shopping festival as consumers tighten their spending in anticipation of difficult economic times ahead. Categories such as sportswear and fitness, however, have been doing well as customers “trade down a Gucci bag for Lululemon sportswear,” he said. Platforms like JD.com and Alibaba, which operates e-commerce platforms Taobao and Tmall, previously used to publish the value of transactions made during the festival, but have since stopped revealing the total figure. While yearly growth used to be in the double digits, estimates of recent figures have dwindled to low single-digit growth. Syntun, a data provider, estimated that last year’s gross merchandising volume sales across major e-commerce platforms grew just 2% to $156.40 billion, a far cry from double-digit growth before COVID-19. Merchants who typically take part in the Singles’ Day shopping festivals say the costs of participation no longer pay off, amid high advertising fees and unsatisfactory sales. Zhao Gao, who owns a garment factory in eastern Zhejiang province, said that after paying advertising costs to e-commerce platforms he would only break even after sales. “The platforms have so many rules for promotions and customers have become more skeptical,” he said. “As a merchant, I no longer participate in the Singles’ Day promotions.” Another merchant, Du Baonian who runs a food company processing mutton in Inner Mongolia, said that overall sales in the past year have fallen 15% as consumers downgraded and reduced consumption. Du said that while he still takes part in the Singles’ Day promotions, the higher expenses do not typically generate returns because of sluggish sales. “We are seeing shrinking revenue, but advertisement on the platform can help us to maintain our leading sales position,” he said, adding that he was considering advertising on more e-commerce platforms to target more consumers. Meanwhile, e-commerce platforms grappling with a slowing domestic market have also turned to overseas markets to seek new growth, offering promotions like global free shipping and allowing merchants to sell globally with ease. Alibaba, for example, said in a blog post on its Alizila site that some 70,000 merchants saw sales double with global free shipping. In markets like Singapore and Hong Kong, new customers also doubled, Alibaba said.

Never underestimate what a single gopher can achieve in a day: one of the burrowing mammals helped boost soil fungi in an area blanketed by ash from the explosive eruption of Mount St Helens in Washington state

In the 1920s, Erwin Schrödinger wrote an equation that predicts how particles-turned-waves should behave. Now, researchers are perfectly recreating those predictions in the lab

Never underestimate what a single gopher can achieve in a day: one of the burrowing mammals helped boost soil fungi in an area blanketed by ash from the explosive eruption of Mount St Helens in Washington state

Title: 6 in 10 of America's Single Guys 'Take Responsibility' for Contraception
Category: Health News
Created: 8/31/2017 12:00:00 AM
Last Editorial Review: 8/31/2017 12:00:00 AM

Retrotransposable elements (RTEs) are common mobile genetic elements comprising ~42% of the human genome. RTEs play critical roles in gene regulation and function, but how they are specifically involved in complex diseases is largely unknown. Here, we investigate the cellular heterogeneity of RTEs using 12 single-cell transcriptome profiles covering three neurodegenerative diseases, Alzheimer's disease (AD), Parkinson's disease, and multiple sclerosis. We identify cell type marker RTEs in neurons, astrocytes, oligodendrocytes, and oligodendrocyte precursor cells that are related to these diseases. The differential expression analysis reveals the landscape of dysregulated RTE expression, especially L1s, in excitatory neurons of multiple neurodegenerative diseases. Machine learning algorithms for predicting cell disease stage using a combination of RTE and gene expression features suggests dynamic regulation of RTEs in AD. Furthermore, we construct a single-cell atlas of retrotransposable elements in neurodegenerative disease (scARE) using these data sets and features. scARE has six feature analysis modules to explore RTE dynamics in a user-defined condition. To our knowledge, scARE represents the first systematic investigation of RTE dynamics at the single-cell level within the context of neurodegenerative diseases.

Single maintenance and reliever therapy (SMART) is an asthma treatment approach that utilizes combined inhaled corticosteroids and long-acting β-agonists for maintenance and quick relief therapy. Despite the evidence for its benefits in asthma treatment and its adoption into American and international asthma guidelines and recommendations, SMART remains a practice of some debate. This article reviews the available evidence for SMART and offers guidance for its integration into comprehensive asthma management. Overall, short-acting β-agonist-only asthma therapy regimens should be avoided, regardless of condition severity (SOR A Recommendation). Family medicine clinicians should start SMART for patients requiring either GINA Step 3 or 4 therapy, especially if they have signs of poor adherence (SOR B Recommendation). Finally, use budesonide-formoterol over other inhaled corticosteroid/long-acting β-agonist combinations when implementing SMART (SOR B Recommendation).

In Canada and across the globe, access to PSMA PET/CT is limited and expensive. For patients with biochemical recurrence (BCR) after treatment for prostate cancer, novel strategies are needed to better stratify patients who may or may not benefit from a PSMA PET scan. The role of the free-to-total prostate-specific antigen (PSA) ratio (FPSAR) in posttreatment prostate cancer, specifically in the PSMA PET/CT era, remains unknown. Our aim in this study was to determine the association of FPSAR in patients referred for ¹⁸F-DCFPyL PSMA PET/CT in the BCR setting and assess the correlation between FPSAR and ¹⁸F-DCFPyL PSMA PET/CT positivity (local recurrence or distant metastases). Methods: This prospective study included 137 patients who were referred for ¹⁸F-DCFPyL PSMA PET/CT and had BCR with a total PSA of less than 1 ng/mL after radical prostatectomy (RP) (including adjuvant or salvage radiotherapy). Blood samples were collected on the day of ¹⁸F-DCFPyL PSMA PET/CT. FPSAR was categorized as less than 0.10 or as 0.10 or more. A positive ¹⁸F-DCFPyL PSMA PET/CT scan was defined by a PROMISE classification lesion score of 2 or 3, irrespective of the site of increased tracer uptake (e.g., prostate, pelvic nodes, bone, or viscera). Results: Overall, 137 blood samples of patients with BCR after RP were analyzed to calculate FPSAR. The median age at ¹⁸F-DCFPyL PSMA PET/CT was 68.6 y (interquartile range, 63.0–72.4 y), and the median PSA at ¹⁸F-DCFPyL PSMA PET/CT was 0.3 ng/mL (interquartile range, 0.3–0.6 ng/mL). Eighty-six patients (62.8%) had an FPSAR of less than 0.10, whereas 51 patients (37.2%) had an FPSAR of 0.10 or more. An FPSAR of 0.10 or more was identified as an independent predictor of a positive ¹⁸F-DCFPyL PSMA PET/CT scan, with an odds ratio of 6.99 (95% CI, 2.96–16.51; P < 0.001). Conclusion: An FPSAR of 0.10 or more after RP independently correlated with increased odds of a positive ¹⁸F-DCFPyL PSMA PET/CT scan among BCR post-RP patients. These findings may offer an inexpensive method by which to triage access to ¹⁸F-DCFPyL PSMA PET/CT in jurisdictions where availability is not replete.