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SAD phasing of XFEL data depends critically on the error model

A nonlinear least-squares method for refining a parametric expression describing the estimated errors of reflection intensities in serial crystallographic (SX) data is presented. This approach, which is similar to that used in the rotation method of crystallographic data collection at synchrotrons, propagates error estimates from photon-counting statistics to the merged data. Here, it is demonstrated that the application of this approach to SX data provides better SAD phasing ability, enabling the autobuilding of a protein structure that had previously failed to be built. Estimating the error in the merged reflection intensities requires the understanding and propagation of all of the sources of error arising from the measurements. One type of error, which is well understood, is the counting error introduced when the detector counts X-ray photons. Thus, if other types of random errors (such as readout noise) as well as uncertainties in systematic corrections (such as from X-ray attenuation) are completely understood, they can be propagated along with the counting error, as appropriate. In practice, most software packages propagate as much error as they know how to model and then include error-adjustment terms that scale the error estimates until they explain the variance among the measurements. If this is performed carefully, then during SAD phasing likelihood-based approaches can make optimal use of these error estimates, increasing the chance of a successful structure solution. In serial crystallography, SAD phasing has remained challenging, with the few examples of de novo protein structure solution each requiring many thousands of diffraction patterns. Here, the effects of different methods of treating the error estimates are estimated and it is shown that using a parametric approach that includes terms proportional to the known experimental uncertainty, the reflection intensity and the squared reflection intensity to improve the error estimates can allow SAD phasing even from weak zinc anomalous signal.




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Shipping industry sends help as project in Panama tackles amphibian crisis

The rescue pods will be part of the project’s Amphibian Rescue Center at Summit Municipal Park, which will also include a lab with a quarantine facility.

The post Shipping industry sends help as project in Panama tackles amphibian crisis appeared first on Smithsonian Insider.




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Facebook friends help scientists quickly identify nearly 5,000 fish specimens collected in Guyana

Faced with insufficient time and inadequate library resources to tackle the problem on their own, they instead posted a catalog of specimen images to Facebook and turned to their network of colleagues for help.

The post Facebook friends help scientists quickly identify nearly 5,000 fish specimens collected in Guyana appeared first on Smithsonian Insider.




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Research team to explore how microbial diversity defends against disease

Researchers who will study the microbial communities living on the skins of frogs that are surviving the fungal scourge of chytridiomycosis, deadly to the frogs.

The post Research team to explore how microbial diversity defends against disease appeared first on Smithsonian Insider.




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Jaguar conservation depends on neighbor attitudes

According to a new survey of residents living near two major national parks in Panama, jaguars deserve increased protection. Nature and wildlife are considered national […]

The post Jaguar conservation depends on neighbor attitudes appeared first on Smithsonian Insider.




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Kids’ video: “Red Bird” – Dan Zanes & Friends

“Red Bird” from Dan Zanes and Friends’ album ‘Lead Belly, Baby’ featuring Ashley Phillips and Shareef Swindell, Smithsonian Folkways Recordings. To learn more click here: […]

The post Kids’ video: “Red Bird” – Dan Zanes & Friends appeared first on Smithsonian Insider.



  • History & Culture
  • Video



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How Carnivorous Plants avoid eating their pollinating insect friends

Carnivorous plants are a fascinating example of nature at its best. Living in habitats with nutrient-poor soil, carnivorous plants evolved to attract some insects as […]

The post How Carnivorous Plants avoid eating their pollinating insect friends appeared first on Smithsonian Insider.




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[GUIDE] How To Chat With Friends On Skype in PSP




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IOM Report Recommends Eight Additional Preventive Health Services to Promote Womens Health

A new report from the Institute of Medicine (IOM) recommends that eight preventive health services for women be added to the services that health plans will cover at no cost to patients under the Patient Protection and Affordable Care Act of 2010 (ACA).




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Report Urges Caution in Handling and Relying Upon Eyewitness Identifications in Criminal Cases, Recommends Best Practices for Law Enforcement and Courts

A new report from the National Research Council recommends best practices that law enforcement agencies and courts should follow to improve the likelihood that eyewitness identifications used in criminal cases will be accurate.




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Increase in the Number of Children Who Receive Federal Disability Benefits for Speech and Language Disorders Similar to Trends in the General Population, Says New Report

The increase in the number of children from low-income families who are receiving federal disability benefits for speech and language disorders over the past decade parallels the rise in the prevalence of these disorders among all U.S. children, says a new report by the National Academies of Sciences, Engineering, and Medicine.




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New Report Recommends Research to Improve Understanding of Relationship Between Fatigue and Crash Risk for Truck and Bus Drivers

Insufficient sleep can decrease a commercial motor vehicle (CMV) driver’s level of alertness, which may increase the risk of a crash, yet little is known about effective ways to minimize that risk, says a new report from the National Academies of Sciences, Engineering, and Medicine.




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New Report Recommends Research Agenda for Effective Science Communication

A new report from the National Academies of Sciences, Engineering, and Medicine highlights the complexity of communicating about science effectively, especially when dealing with contentious issues, and proposes a research agenda to help science communicators and researchers identify effective methods.




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Report Recommends New Framework for Estimating the Social Cost of Carbon

To estimate the social cost of carbon dioxide for use in regulatory impact analyses, the federal government should use a new framework that would strengthen the scientific basis, provide greater transparency, and improve characterization of the uncertainties of the estimates, says a new report by the National Academies of Sciences, Engineering, and Medicine.




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New Report Recommends Priority Actions to Achieve Global Health Security, Protect U.S. Position as Global Health Leader

A new report from the National Academies of Sciences, Engineering, and Medicine identifies global health priorities in light of current and emerging challenges and makes 14 recommendations for the U.S. government and other stakeholders to address these challenges, while maintaining U.S. status as a world leader in global health.




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New Report Finds FMCSA’s Safety Measurement System to Be Conceptually Sound, Recommends Implementation Improvements

While the Federal Motor Carrier Safety Administration’s (FMCSA) Safety Measurement System (SMS) used to identify commercial motor vehicle carriers at high risk for future crashes is conceptually sound, several features of its implementation need improvement, says a new congressionally mandated report from the National Academies of Sciences, Engineering, and Medicine.




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New Report Recommends Construction of Four New Polar Icebreakers of the Same Design as the Lowest-Cost Strategy for Protecting U.S. Interests in Arctic and Antarctic

The U.S. lacks icebreaking capability in the Arctic and Antarctic and should build four polar icebreakers with heavy icebreaking capability to help minimize the life-cycle costs of icebreaker acquisition and operations, says a new congressionally mandated letter report from the National Academies of Sciences, Engineering, and Medicine.




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New Report Recommends Methods and Guiding Principles for Developing Dietary Reference Intakes Based on Chronic Disease

A new report from the National Academies of Sciences, Engineering, and Medicine outlines how to examine whether specific levels of nutrients or other food substances (NOFSs) can ameliorate the risk of chronic disease and recommends ways to develop dietary reference intakes (DRI) based on chronic disease outcomes.




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New Report Recommends Changes to County Crop and Cash Rent Estimation Methods Used by the National Agricultural Statistics Service

Producing more precise county-level estimates of crops and farmland cash rents will require integrating multiple data sources using model-based predictions that are more transparent and reproducible, says a new report from the National Academies of Sciences, Engineering, and Medicine.




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The Quality of Abortion Care Depends on Where a Woman Lives, Says One of Most Comprehensive Reviews of Research on Safety and Quality of Abortion Care in the U.S.

While legal abortions in the U.S. are safe, the likelihood that women will receive the type of abortion services that best meet their needs varies considerably depending on where they live, says a new report from the National Academies of Sciences, Engineering, and Medicine.




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New Report Recommends a Nationwide Effort to Better Estimate Methane Emissions

The U.S. should take bold steps to improve measurement, monitoring, and inventories of methane emissions caused by human activities, says a new report from the National Academies of Sciences, Engineering, and Medicine.




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Academic Institutions Should Prepare Undergraduates for a Data-Driven Workplace, New Report Recommends

All U.S. undergraduate students should develop a basic understanding of data science to prepare them adequately for the workforce, says a new report from the National Academies of Sciences, Engineering, and Medicine.




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New Report Says Individual Research Results Should Be Shared With Participants More Often - Recommends Framework for Decision-Making

When conducting research involving the testing of human biospecimens, investigators and their institutions should routinely consider whether and how to return individual research results on a study-specific basis through an informed decision-making process, says a new report from the National Academies of Sciences, Engineering, and Medicine.




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New Report Examines Reproducibility and Replicability in Science, Recommends Ways to Improve Transparency and Rigor in Research

While computational reproducibility in scientific research is generally expected when the original data and code are available, lack of ability to replicate a previous study -- or obtain consistent results looking at the same scientific question but with different data -- is more nuanced and occasionally can aid in the process of scientific discovery, says a new congressionally mandated report from the National Academies of Sciences, Engineering, and Medicine.




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New Report Recommends Ways to Strengthen the Resilience of Supply Chains After Hurricanes, Based on Lessons Learned From Hurricanes Harvey, Irma, Maria

A new report from the National Academies of Sciences, Engineering, and Medicine recommends ways to make supply chains -- the systems that provide populations with critical goods and services, such as food and water, gasoline, and pharmaceuticals and medical supplies – more resilient in the face of hurricanes and other disasters, drawing upon lessons learned from the 2017 hurricanes Harvey, Irma, and Maria.




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Mercado Pago launches feature to split payments between friends

Argentina-based fintech Mercado Pago has announced it will now be possible to share...




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In Parkland, Another Senior Year Ends In Turmoil. But This Time, 'It's Not Just Us'

; Credit: /Dani Pendergast for NPR

Caitie Switalski | NPR

Friday, March 13, was the last time Alexandra Sullivan saw her fellow yearbook staffers in person.

"We were trying to get as many pictures of people as possible 'cause we knew we wouldn't be able to take any more," Sullivan, 18, says.

Like most U.S. public school students, Sullivan is learning from home now. And much like her lessons, her work on the yearbook continues.

Sullivan is the yearbook profiles editor at Marjory Stoneman Douglas High School in Parkland, Fla. She's one of 10 seniors who were also on staff two years ago, when a gunman opened fire at their school. Back then, she and her classmates had to adapt to an unimaginable tragedy. Now, they have to adapt again – this time, to the pandemic.

"This book has to get done and we'll do whatever we have to do to finish it," she says, "which is exactly how we approached the '18 book."

Senior Caitlynn Tibbetts, the yearbook's co-editor-in-chief, was also on staff when the shooting happened. She says there's a collective grief among seniors over what their class — which has already lost so much — is losing now. They won't be able to dance together at prom, or walk across the stage at graduation.

"This class especially has gotten screwed over so much through the past four years," Tibbetts, 18, says. "The last two months were supposed to be the best, and they were supposed to make up for everything that we've been through. And it's really hard on us to kind of just watch it all disappear."

Amid all the uncertainty, she says, one thing is clear: The yearbook must get done, and it must get to students.

High school yearbooks are like time capsules. They record theater productions, which teams went to state finals, who was voted most likely to succeed.

And when a news event makes history – leaving a mark on students and society – it's the yearbook's job to document it. At Stoneman Douglas, that's meant changing plans just weeks before the yearbook is due.

Yearbook advisor Sarah Lerner says, "Having done one under unthinkable circumstances before, I hate to say that we're kind of, you know, used to it, but, for the seniors on staff, we are."

Two years ago, after the shooting, the yearbook staff pivoted to include remembrances of the victims. Tibbetts and Sullivan stepped up to help write them, and anything else that was needed at the last minute, while other yearbook staffers took time to attend funerals.

This year, they're making room for two new spreads about the pandemic.

"One of them is more of a factual-based one, how it's affected our community, including businesses," Tibbetts explains. "The other spread is focused on the effect it's had on us personally, both with online schooling and especially with seniors."

Logistically, putting the yearbook together and writing the new sections has been a challenge. Unlike 2018, they can't be in the same room with each other to finish the design.

"We have to social distance and our parents wouldn't let us go out," Tibbetts says.

They mainly rely on a group chat with everyone on the staff. "It can get hectic," Tibbetts says, "especially when it's all happening at like 12 a.m."

Lerner and her students missed the original deadline to finish the book, on April 6. But the printer, Walsworth, says the company is being flexible with Stoneman Douglas and other yearbook staffs across the country. Lerner says she's aiming to get the book in by the end of April.

Once the printed copies come back, more than 1,200 books will somehow have to be distributed to students. Lerner has some ideas for how to do that safely.

However, there's one important yearbook tradition they may not be able to save.

"We may not actually get to sign books this year," Lerner says. And that's been hard to accept.

"As a teacher, I really like to get my students to sign my book, you know, and I like to sign theirs and I like to see the kids carrying them around at school."

Lerner says she's sad that might not happen this year. But at least this time, the students of Marjory Stoneman Douglas High School aren't on their own.

"Unlike the 2018 books, this situation is not unique to us," she says. "So there's comfort in knowing that all staffs are going through the same issue. It's not just us."

Copyright 2020 WLRN 91.3 FM. To see more, visit WLRN 91.3 FM.

This content is from Southern California Public Radio. View the original story at SCPR.org.




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30% off GridinSoft Trojan Killer - Ends July 3rd, 2014




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50% discount off a 2 years license of Trojan Killer - Ends August 10, 2014




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New Deal: Aduro Surge Protector: 6-Outlet & 2-USB Port at 52% off. Ends Oct. 3rd




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A happy neighbourhood depends on interplay of local environmental features

A new German study suggests that residents??? level of satisfaction with their urban neighbourhood can be predicted from their perceptions of multiple and co-occurring burdens, such as poor air quality, lack of green space, noise and low cleanliness.




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WHO recommends setting night noise limits at 40 decibels

The World Health Organisation (WHO) has set the European target limit of outdoor night noise levels at annual average of 40 decibels (dB) in its new guidelines. This would protect the public, including the most vulnerable, such as children and the elderly.




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A circular economy for mobile phones: study recommends improved waste collection and longer lifespans for handsets

Around 50% of gold in used mobile phones is not recovered for future use, a new study finds. The researchers suggest that a global circular economy in mobile phones could be created by improving recycling of precious metals in phones in developing countries, as well as increasing the lifespan of phones and improving collection after use. These changes will reduce pressures on non-renewable resources and close ‘metal flow loops’.




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Indian IT leans on govt spends, managed services as deals slow

IT advisory IDC estimates that domestic IT spending will decline 4.5% to $55.5 billion this fiscal




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Covid-19 Heroes: Digital-led approach has reaped rich dividends for MG Motors

Digital initiatives that began pre lockdown are giving the carmarker confidence that it will emerge stronger when the dust settles.




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Relationships with family members, but not friends, decrease likelihood of death

For older adults, having more or closer family members in one's social network decreases his or her likelihood of death, but having a larger or closer group of friends does not, finds a new study that will be presented at the 111th Annual Meeting of the American Sociological Association (ASA).

read more



  • Psychology & Sociology

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EU migration under environmental change: impact depends on current infrastructure

Environmental changes in the future, such as an increase in floods, land degradation and drought could result in changes in migration patterns in Europe, researchers write in a recent analysis. It is difficult to predict these exact migration patterns, however, as they are determined by a complex interplay of economic, political and social factors with environmental change, as well as adaptive capacity.




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Food security depends on sustainable nutrient management of soils

Food security is being threatened by loss of soil nutrients that are essential for the high yield of crops. A recent study outlines strategies to ensure the sustainable production of food through a holistic approach to soil nutrient management.




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Implementation of innovative, resource-efficient urban water systems depends on wide-ranging cooperation

New technology that makes energy capture from waste water and re-use of grey water possible can contribute to energy- and resource efficiency — but the widespread application of such technology requires a new, collaborative approach, shows a new study. Taking radical innovation in urban water systems beyond the pilot stage will require cooperation between a variety of stakeholders, suggest the findings of expert interviews and workshops.




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How Facebook’s Reliance deal upends a $1 trillion digital arena

Reliance and Facebook know a friction-less payments service is key to successful online commerce — but so are mom and pop shops. JioMart and WhatsApp’s embryonic Mumbai service is intended to first get Indians accustomed to messaged transactions with local businesses known as kirana: the tiny neighborhood stores where most Indians buy daily essentials.




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Indian IT leans on govt spends, managed services as deals slow

IT advisory IDC estimates that domestic IT spending will decline 4.5% to $55.5 billion this fiscal




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Tech trends which are transforming the fashion retail sector

India has tried its hands out in 4.0 technologies to pace up with the rapidly changing retail landscape. The use of AI, ML and data analytics has increased which are being used in functions like BOPIS, Click & Collect and Self-Kiosk.




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Banking analytics trends to look in 2020

The future challenge is to develop analytic strengths that span the organisation and not just areas of expertise.




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Successful re-introduction of waterfowl depends on month of release

New EU-funded research has provided useful guidelines for re-introducing threatened species, using a waterfowl case study. The Spanish researchers developed a method for deciding the best time of year to release the species into their original habitat.




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Improved population trends for Eastern European birds protected by recent legislation

National bird conservation policies implemented in Eastern Europe in the 1990s have substantially benefited bird populations across the region, a new study has shown. Compared with the period 1970-1990, the population trends of species protected by national legislation improved during 1990-2000, particularly for those species receiving focused attention. Across the 306 species studied, the average rate of decline was much lower after protection than before. This suggests that modern conservation policies in the region were already taking effect.




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Are endocrine disrupting chemicals responsible for downward trends in male fertility?

A growing body of evidence suggests that endocrine-disrupting compounds (EDCs) are contributing to declines in fertility. This case-control study found that EDCs were associated with changes to sex hormones and risk of subfertility in men. The researchers say environmental levels of these chemicals should be reduced to protect male fertility.




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Chemicals risk assessment: Baltic study recommends more monitoring of emerging pollutants

Chemicals are everywhere and new substances are regularly being introduced to the market. However, only some pose a risk to the environment. How do we decide which of them to monitor? A new study using a database of chemicals found in fish in the Baltic Sea has assessed which chemicals are commonly monitored. The researchers suggest that monitoring is biased towards known, already regulated hazardous chemicals, and recommend changes to address other chemicals.