I have a close circle of clued-on/tech savvy friends whose views I take seriously. For the last few weeks, one of these friends has been sending me emails extolling the merits of something called IOTA – which calls itself as the next generation Blockchain.  At first, I thought of IOTA as yet another cryptocurrency. A [...]

We are launching an AI lab. The goal is to learn unsupervised learning through Robotics (Cobots) Long seen as a poor cousin to supervised learning -  with Variational autoencoders, Reinforcement learning and  Generative-Adversarial networks , unsupervised learning techniques have moved beyond the limitations of autoencoders. From Oct 2018 to March 2019 , we are running a [...]

News24 brings you the top stories of the day, summarised into neat little packages. Read through quickly or listen to the articles via our customised text-to-speech feature.

While the DA may have found that George Deputy Mayor Raybin Figland had no criminal liability for allegedly sexting a teenage pupil – the Public Protector's office could still probe him for misconduct.

International Relations and Cooperation Minister Ronald Lamola threw diplomacy out the window on Tuesday and responded angrily when he was asked whether South Africa and the ANC were "protecting its friend" Frelimo in troubled Mozambique.

The Gauteng education department has postponed the appointment of new office-based staff as well as teachers who applied to be promoted as heads of department, deputy principals and principals until April because of budget constraints.

The World Meteorological Organisation (WMO) has raised the alarm over climate change, reporting 2024 is the world's hottest year yet.

Modern organizations regard data as a strategic asset that drives efficiency, enhances decision making, and creates new value for customers. Across the organization—product management, marketing, operations, finance, and more—teams are overflowing with ideas on how data can elevate the business. To bring these ideas to life, companies are eagerly hiring data scientists for their technical […]

Jon Stewart tears apart the denials and false claims Trump supporters make about their terrible candidate and wonders how anyone can remain undecided.

At the top of this piece by Stewart sits the strange claim MAGAs keep making that Vice President Harris isn't clear about her plans. — Read the rest

The post Jon Stewart on the bewildering denial in remaining an undecided voter appeared first on Boing Boing.

There are laptops sold with NVidia's high-end 4090 graphics chip, but it's a power-constrained mobile version appropriate for portable machines. A Chinese-language blogger modded themselves a beast of a machine with a full desktop-class 4090 card. This thing weighs 15 pounds and its battery life is a Borgesian map of nothing (one must plug it in). — Read the rest

The post "Laptop" with full 4090 GPU weighs 15 pounds appeared first on Boing Boing.

Update: Surveillance footage of the shooting shown in court

Letcher County Sheriff Shawn "Mickey" Stines shot dead District Judge Kevin Mullins in his chambers, was charged with first-degree murder, and has pleaded not guilty. It soon became apparent that Stines had no intention of resigning, despite the inconvenience of his incarceration, leaving authorities to deal with the long and difficult process of removing him from office. — Read the rest

The post Kentucky Sheriff who killed judge plans to retire; footage of shooting shown in court appeared first on Boing Boing.

An underwater rover deployed by the Minderoo-UWA Deep-Sea Research Centre captured remarkable footage 3,300-meters down at the bottom of the Tonga Trench. It shows a rarely-seen bigfin squid (Magnapinna) "taking a walk" on its 13-foot tentacles. Watch below.

There have only been around 20 documented sightings of this beautifully bizarre creature in two decades. — Read the rest

The post Deep sea video of weird sea creature walking around on its 13-foot "legs" appeared first on Boing Boing.

Time's arrow may not be as unidirectional as we were led to believe. According to Scientific American, a group of quantum physical researchers at the University of Toronto had observed evidence of what they're calling "negative time"—specifically, of photons exiting a material before they ever entered it, to begin with. — Read the rest

The post Physicists find evidence of "negative time" in photons appeared first on Boing Boing.

A Boring Day's DIY head speaker is a one-of-a-kind speaker straight out of the uncanny valley. It's fantastic. 

The speaker is built inside of a plastic mannequin head, with exposed wires that look like the mannequin's hairdo. The sound comes out of the mannequins eye holes which look like bug eyes.To — Read the rest

The post Bluetooth speaker inside a mannequin head appeared first on Boing Boing.

Surveillance footage of Sheriff Shawn "Mickey" Stines shooting and killing District Judge Kevin Mullins in his own chambers was played in a Kentucky court yesterday, shocking onlookers. Judge Rupert Wilhoit found probable cause to refer Stines' first-degree murder charge to a grand jury. — Read the rest

The post Surveillance footage of Kentucky Sheriff killing judge shown in court appeared first on Boing Boing.

Nintendo is cracking down on emulators of its hardware and media depicting their use. First Yuzu was taken down with a lawsuit, and now departs Ryujinx, a similar project that had sought to avoid the legal landmines Yuzu stepped on. — Read the rest

The post Another emulation project disappears amid Nintendo crackdown appeared first on Boing Boing.

Announcing the brand new Tom the Dancing Bug book: Volume 8 of The Complete Tom the Dancing Bug book program is "IT'S THE GREAT STORM, TOM THE DANCING BUG!" Now accepting orders right HERE! Get your personalized / signed / sketched / swagged copy today! — Read the rest

The post Tom the Dancing Bug: "Hey, Ladies! Trump will be your protector!" appeared first on Boing Boing.

Jason M. Allen's "Theatre D'Opera Spatial" won an art competition at the 2022 Colorado State Fair, but the work was subsequently denied copyright protection due to his use of AI software to generate it and his unwillingness to disclaim that contribution to the whole. — Read the rest

The post AI artist appeals denial of copyright protection appeared first on Boing Boing.

A North Texas man recently spent $4000 on a guitar he believed was signed by Taylor Swift at the Ellis County Wild Game Dinner in Waxahachie, Texas, and then immediately destroyed it with a hammer. Sounds like the guitar was an imposter. — Read the rest

The post Texas man wastes $4k smashing a Taylor Swift "themed" guitar appeared first on Boing Boing.

When the Baby Boomers retire they are going to be taking their substantial knowledge with them. The younger generation to whom that knowledge should be transferred likes to consume video.  This has some very interesting implications for organizations that want to capture that knowledge, organize it, and make it easy for employees to find it and benefit from it.

Here is a link to a webcast on June 1 where Dan Rasmus, a technology strategist and author of Listening to the Future , will discuss the potential for video to serve as an all important medium for knowledge transfer and organizational learning.

Topics to be Covered:
• Knowledge Acquisition via Video
• Video as a Tool for Knowledge Management
• Use of Video or Operational Efficiency
• Practical Applications of Video for Learning in the Enterprise

Click here to register for the webcast

What Is "Demure and Mindful" to Real Estate?

The following article, Biden’s Corrupt FEMA Told Workers Not to Help Hurricane Victims Who Had Trump Signs, was first published on Conservative Firing Line.

Joe Biden’s corrupt Federal Emergency Management Administration (FEMA) has been caught telling its on-the-ground operatives not to help anyone with a Donald Trump campaign sign in their yard. The news broke late last week when a whistleblower revealed agency messages that told workers to refuse to help Trump supporters in the wake of Hurricane Milton …

Continue reading Biden’s Corrupt FEMA Told Workers Not to Help Hurricane Victims Who Had Trump Signs ...

The following article, Forty-Seven Percent Of Harris Voters Believe Trump Will Not Be A Legitimate President; 54 Percent Want To Leave The Country, was first published on Conservative Firing Line.

I really wish that everyone would just calm down.  Emotions always run high immediately after an election, but what we are witnessing this time around is truly frightening.  We live at a time when people feel free to express their deepest, darkest emotions on social media, and right now “freak out video” after “freak out …

Continue reading Forty-Seven Percent Of Harris Voters Believe Trump Will Not Be A Legitimate President; 54 Percent Want To Leave The Country ...

A couple weeks ago, my pals at Twitter were kind enough to invite me in to visit with their (rapidly growing) team. The topic was meetings, so I used it as an opportunity to publicly premiere a talk I've been presenting to private clients over the past few months.

I hope you'll enjoy, Broken Meetings (and how you'll fix them).

Slides:

Supplementary links and commentary forthcoming, but I wanted to go ahead and post the talk as quickly as the video was available. Special thanks to Michelle, Jeremy, and the crackerjack Twitter crew for a swell afternoon.

I really like this talk and sincerely hope you will find it useful in helping to un-break your own meetings.

[update 2011-01-18 @ 16:07:40: We're up!]

5by5 Live

Before Christ was a corporal, Dan Benjamin was already a bit of a hero to me.

Since the early aughts–long before his insanely great 5by5.tv podcast network–Dan’s Hivelogic Enkoder was saving us millions of spam messages. His thoughtful tutorials on OS X (including unmissable advice on doing sane installs of MySQL and Rails, among others) are among the best on the web. His CSS has been widely stolen and reused without acknowledgment by thieves as diverse as other people and me. And his polymath posts on everything from Buddhism to The Paleo Diet to how to record a “Double-ender” have shown a charming combination of curiosity and empathy that, amongst numerous other reasons, clearly makes Dan a better human than me.

A propos of nothing, Dan’s also the guy who conducted one of (mp3) the three best interviews with me in which it’s been my good fortune to participate.1

Today, I’m honored to say that Dan and I are starting a thing together.

If it suits you, drop by 5by5.tv/live in about 35 minutes–at Noon Eastern/9am Pacific–to find out what we’re up to. I think it might be good. I’ll just say I’m as excited about this as I’ve been about any new project I’ve started in the past year or so.

Anyway. You can judge for yourself. Whether you can tolerate me or otherwise, definitely do not miss the work Dan’s doing at 5by5. Because it really is outstanding and very polished stuff.

As for our thing? My own goal, to paraphrase a bit from that interview with Dan, is to help you get excited, get better–and then?–Back to Work.

More soon. Thanks.

And...we're up

Back to Work | Ep.#1: Alligator in the Bathroom

Download MP3 of "'Back to Work,' Ep. 1"

In the inaugural episode of Back to Work, Merlin Mann and Dan Benjamin discuss why they’re doing this show, getting back to work instead of buying berets, the lizard brain, and compare the Shadow of the Mouse to San Francisco, and eventually get to some practical tips for removing friction.

It's a start.

Sexy Audio RSS Feed
Sexy Subscription via iTunes

Episode Links

• Welcome to BrettTerpstra.com, home of Brett Terpstra and his nerdery
• carlhuda/janus – GitHub
• practically efficient — technology, workflows, life
• MacSparky – Blog
• The Brooks Review
• And now it’s all this
• Dan Rodney’s List of Mac OS X Keyboard Shortcuts & Keystrokes
• 43f Podcast: John Gruber & Merlin Mann’s Blogging Panel at SxSW | 43 Folders
• waffle software · ThisService
• One Thing Well
• html2text: THE ASCIINATOR (aka html2txt)
• The Conversation #27: Missionless Statements – 5by5

Have you heard of Locorotondo, Italy? Locorotondo is one of Southern Italy’s most beautiful villages, in my opinion, but it isn’t yet overwhelmed with tourist crowds. A true hidden gem, Locorotondo is just a 12-minute drive from busy Alberobello in the Puglia region, but feels like a world away. Planning your trip to Locorotondo last […]

The post Visiting Locorotondo, Italy – A Guide to the Trulli Town appeared first on Adventurous Kate.

If killjoy leftists can wear their self-parodying and ridiculous 'Handmaid's Tale' outfits, then they can't complain when a MAGA voter plays the same game... only better.

The post MUST-WATCH: MAGA Voter Finds Hilarious Way To Sidestep Election Clothing Rules appeared first on Clash Daily.

The vote was a bloodless revolution that showed us that The People want traditionalism, conservatism, populism, and nationalism, i.e., liberty and justice for all.

The post The Vote & The Implications appeared first on Clash Daily.

Did you know that the Marines just celebrated their 249th birthday? This is one helluva tribute.

The post VETERAN’S DAY: You’re Gonna Love What One Patriotic Coffee Company Did For The Marines! appeared first on Clash Daily.

You know it's bad when you give the Democrats a whoopin and the corprate media clowns have no choice but to concede your point.

The post GLORIOUS: Scott Jennings Gets Van Jones To Cry ‘Uncle’ Live On CNN appeared first on Clash Daily.

HEY uses a branch deploy system that I’ve written about here on SvN and talked about frequently on Twitter. Plenty of other companies have implemented their own version of branch deploys (typically under a different name), but this was my own implementation, so I’m proud of it. First, a primer on how it works: Developer… keep reading

Chairman Klein and members of the Senate Industry, Business and Labor Committee- My name is David Heinemeier Hansson, and I’m the CTO and co-founder of Basecamp, a small internet company from Chicago that sells project-management software and email services. I first testified on the topic of big tech monopolies at the House Antitrust Subcommittee’s field… keep reading

There appeared to be a definite frost between first lady Jill Biden and Vice President Kamala Harris during a Veterans Day event at Arlington National Cemetery on Monday. A close-up […]

The post Footage of Jill Biden and Kamala Harris Sitting Side-by-Side at Veterans Day Event Goes Viral appeared first on The Western Journal.

Conservative activist Scott Presler, whose organization Early Vote Action helped swing Pennsylvania to the right in the general election, announced Monday he will be working to do the same in […]

The post Democrats Have Another Problem to Worry About as Man Who Helped Trump Win Has Eyes on Blue State appeared first on The Western Journal.

CNN reportedly will be making more cuts in the near future, including to its highly paid on-air personalities, following poor election ratings. The New York Post reported that Fox News […]

The post On-Air Talent Will Not Be Spared as CNN Prepares Mass Layoffs in Wake of Election: Report appeared first on The Western Journal.

President-elect Donald Trump is reportedly considering lawyer Alina Habba to be the White House press secretary. Habba often spoke to the media while she was on the legal team representing […]

The post Report Shows New Front-Runner for Trump's Press Secretary Spot: The Media Should Be Terrified appeared first on The Western Journal.

Now that there is a Republican majority in the next Congress, it’s time for the party to bare its internal fault lines. That is likely to take place Wednesday, according […]

The post Another House Speaker Battle? Mike Johnson's Position May Be at Risk as Conservative Anger Heats Up appeared first on The Western Journal.

Sir Kenny Dalglish hailed nine-time Major champion Gary Player and expressed his delight at Liverpool's position at the top of the Premier League during his visit to South Africa this weekend.

Sri Lanka's cricket board named former South African batsman Neil McKenzie as a consultant coach ahead of their Test tour of South Africa.

The first battle cry has sounded ahead of the showdown at Twickenham on Saturday, with English winger Tommy Freeman reckoning that the Springboks are 'still human' and 'still playing the same game'.

Presented by the Middle East Forum on Nov. 18, 2022 Panel discussion with Daniel Pipes, Cynthia Farahat, and Jonathan Schanzer:

Originally published in Gatestone Institute on Nov. 25, 2022 By Cynthia Farahat Bahgat Saber, a member of the Muslim Brotherhood, operates from his New York apartment and often streams live videos from Times Square. During his multi-hour videos, Saber routinely incites terrorism, assassinations, kidnapping and torture in an extremely graphic manner. The calls for violence […]

By SADF – Nov. 30, 2022 Online conversation on The West, a New Haven for the ‘Muslim Brotherhood’s Planetary Nebula’? On November the 29th at 16H.00 (CET), SADF promoted an online conversation in the web platform StreamYard titled ‘The West, a New Haven for the ‘Muslim Brotherhood’s Planetary Nebula’?’ The conversation included the Member of the European Parliament (EPP, […]

By Walter Donway, Savvy Street originally published on Dec. 4, 2022 Book Review: The Secret Apparatus: The Muslim Brotherhood’s Industry of Death by Cynthia Farahat, Post Hill Press, Bombardier Books (2022) A grim irony of twentieth-century history (but, of course, also predating it) is that the most horrendous threats to humanity have simply been too monstrously evil […]

By Cynthia Farahat On Friday 27th, 2023 at 11:00 am EST, my sweet dear younger brother Amir Farahat passed away. Amir was the most loving father I have ever seen, a great brother, son, husband, and brother-in-law. My little Miró, my little prince, my soul is shattered by your loss. I will forever mourn your […]

Richard Moeckel and Richard Montgomery
J. Amer. Math. Soc. 38 (), 225-241.
Abstract, references and article information

Nonphotochemical quenching (NPQ) is a mechanism of regulating light harvesting that protects the photosynthetic apparatus from photodamage by dissipating excess absorbed excitation energy as heat. In higher plants, the major light-harvesting antenna complex (LHCII) of photosystem (PS) II is directly involved in NPQ. The aggregation of LHCII is proposed to be involved in quenching. However, the lack of success in isolating native LHCII aggregates has limited the direct interrogation of this process. The isolation of LHCII in its native state from thylakoid membranes has been problematic because of the use of detergent, which tends to dissociate loosely bound proteins, and the abundance of pigment–protein complexes (e.g. PSI and PSII) embedded in the photosynthetic membrane, which hinders the preparation of aggregated LHCII. Here, we used a novel purification method employing detergent and amphipols to entrap LHCII in its natural states. To enrich the photosynthetic membrane with the major LHCII, we used Arabidopsis thaliana plants lacking the PSII minor antenna complexes (NoM), treated with lincomycin to inhibit the synthesis of PSI and PSII core proteins. Using sucrose density gradients, we succeeded in isolating the trimeric and aggregated forms of LHCII antenna. Violaxanthin- and zeaxanthin-enriched complexes were investigated in dark-adapted, NPQ, and dark recovery states. Zeaxanthin-enriched antenna complexes showed the greatest amount of aggregated LHCII. Notably, the amount of aggregated LHCII decreased upon relaxation of NPQ. Employing this novel preparative method, we obtained a direct evidence for the role of in vivo LHCII aggregation in NPQ.

China’s zero-COVID cannot continue, reopening is needed Expert comment NCapeling 1 December 2022

Unsure of how to loosen rules, local officials have doubled down on severe restrictions behind so much economic damage. Reopening will quell public anger.

When President Xi Jinping was seen unmasked at the G20 summit in Indonesia, he maintained a largely positive tone with President Joe Biden and other world leaders. This left an impression that China was on the verge of withdrawing its zero-COVID strategy.

A set of loosening policy measures introduced by Beijing seemed to further suggest that China was on track to reopen. As outlined in the 20th party congress, Xi wants to forge a pathway towards economic modernization and this means building economic resilience and a further increase in household incomes.

However, a series of displays of public defiance against the government’s zero-COVID policy has left the rest of the world perplexed. Some loosening of restrictions announced on 11 November by the central government, with the number of new cases still rising rapidly, left the provincial governments in confusion as to which direction to turn.

Absolute political loyalty

The persistent slogan of ‘zero-COVID’ was in stark contrast to the slackening of restrictions. Local officials decided to double down on stringent COVID measures as a way of displaying absolute political loyalty to the top, which inevitably caused daily agonies among large parts of the Chinese population.

This decision-making process sheds some light on the way the Chinese bureaucracy approaches crises at a time when the party leadership is tightening political control. Lower-level officials avoided making important decisions and instead decided to wait for instructions from the top. As the rules were unclear, they implemented policies according to past precedents, in this case zero-COVID, which had worked relatively well for China in 2020 and 2021.

For decades, local governments have been major political actors in China and have known what works best under local conditions. But with tighter regulation being exercised by lower-level bureaucrats and civil servants, there is less opportunity for the input of local knowledge, increasing the risk of ineffective policies being implemented.

Away from Beijing, those not employed by the state have been hit particularly hard by zero-COVID measures. The strategy has intensified economic pressures, exacerbated rising levels of youth unemployment, and tested the patience of the entire country.

Billions of Chinese people want to have their life return to normal without the fear of having the wrong colour – yellow or red – on their COVID health QR code or endless mandatory testing. Only a green code shows that a person is healthy and able to move around freely.

Economy is suffering

China is still balancing its twin aims of containing the spread of COVID and re-engineering its economy along similar lines to the rest of the world. It is difficult to see how China’s economy can crank up again until the country reduces its internal restrictions and reconnects with the world.

An important mechanism of resistance to β-lactam antibiotics is via their β-lactamase–catalyzed hydrolysis. Recent work has shown that, in addition to the established hydrolysis products, the reaction of the class D nucleophilic serine β-lactamases (SBLs) with carbapenems also produces β-lactones. We report studies on the factors determining β-lactone formation by class D SBLs. We show that variations in hydrophobic residues at the active site of class D SBLs (i.e. Trp105, Val120, and Leu158, using OXA-48 numbering) impact on the relative levels of β-lactones and hydrolysis products formed. Some variants, i.e. the OXA-48 V120L and OXA-23 V128L variants, catalyze increased β-lactone formation compared with the WT enzymes. The results of kinetic and product studies reveal that variations of residues other than those directly involved in catalysis, including those arising from clinically observed mutations, can alter the reaction outcome of class D SBL catalysis. NMR studies show that some class D SBL variants catalyze formation of β-lactones from all clinically relevant carbapenems regardless of the presence or absence of a 1β-methyl substituent. Analysis of reported crystal structures for carbapenem-derived acyl-enzyme complexes reveals preferred conformations for hydrolysis and β-lactone formation. The observation of increased β-lactone formation by class D SBL variants, including the clinically observed carbapenemase OXA-48 V120L, supports the proposal that class D SBL-catalyzed rearrangement of β-lactams to β-lactones is important as a resistance mechanism.

Numerous iron-sulfur (Fe-S) proteins with diverse functions are present in the matrix and respiratory chain complexes of mitochondria. Although [4Fe-4S] clusters are the most common type of Fe-S cluster in mitochondria, the molecular mechanism of [4Fe-4S] cluster assembly and insertion into target proteins by the mitochondrial iron-sulfur cluster (ISC) maturation system is not well-understood. Here we report a detailed characterization of two late-acting Fe-S cluster-carrier proteins from Arabidopsis thaliana, NFU4 and NFU5. Yeast two-hybrid and bimolecular fluorescence complementation studies demonstrated interaction of both the NFU4 and NFU5 proteins with the ISCA class of Fe-S carrier proteins. Recombinant NFU4 and NFU5 were purified as apo-proteins after expression in Escherichia coli. In vitro Fe-S cluster reconstitution led to the insertion of one [4Fe-4S]2+ cluster per homodimer as determined by UV-visible absorption/CD, resonance Raman and EPR spectroscopy, and analytical studies. Cluster transfer reactions, monitored by UV-visible absorption and CD spectroscopy, showed that a [4Fe-4S]2+ cluster-bound ISCA1a/2 heterodimer is effective in transferring [4Fe-4S]2+ clusters to both NFU4 and NFU5 with negligible back reaction. In addition, [4Fe-4S]2+ cluster-bound ISCA1a/2, NFU4, and NFU5 were all found to be effective [4Fe-4S]2+ cluster donors for maturation of the mitochondrial apo-aconitase 2 as assessed by enzyme activity measurements. The results demonstrate rapid, unidirectional, and quantitative [4Fe-4S]2+ cluster transfer from ISCA1a/2 to NFU4 or NFU5 that further delineates their respective positions in the plant ISC machinery and their contributions to the maturation of client [4Fe-4S] cluster-containing proteins.