Nanocrystallography has transformed our ability to interrogate the atomic structures of proteins, peptides, organic molecules and materials. By probing atomic level details in ordered sub-10 nm regions of nanocrystals, scanning nanobeam electron diffraction extends the reach of nanocrystallography and in principle obviates the need for diffraction from large portions of one or more crystals. Scanning nanobeam electron diffraction is now applied to determine atomic structures from digitally defined regions of beam-sensitive peptide nanocrystals. Using a direct electron detector, thousands of sparse diffraction patterns over multiple orientations of a given crystal are recorded. Each pattern is assigned to a specific location on a single nanocrystal with axial, lateral and angular coordinates. This approach yields a collection of patterns that represent a tilt series across an angular wedge of reciprocal space: a scanning nanobeam diffraction tomogram. Using this diffraction tomogram, intensities can be digitally extracted from any desired region of a scan in real or diffraction space, exclusive of all other scanned points. Intensities from multiple regions of a crystal or from multiple crystals can be merged to increase data completeness and mitigate missing wedges. It is demonstrated that merged intensities from digitally defined regions of two crystals of a segment from the OsPYL/RCAR5 protein produce fragment-based ab initio solutions that can be refined to atomic resolution, analogous to structures determined by selected-area electron diffraction. In allowing atomic structures to now be determined from digitally outlined regions of a nanocrystal, scanning nanobeam diffraction tomography breaks new ground in nanocrystallography.

Digitonin has long been used as a mild detergent for extracting proteins from membranes for structure and function studies. As supplied commercially, digitonin is inhomogeneous and requires lengthy pre-treatment for reliable downstream use. Glyco-diosgenin (GDN) is a recently introduced synthetic surfactant with features that mimic digitonin. It is available in homogeneously pure form. GDN is proving to be a useful detergent, particularly in the area of single-particle cryo-electron microscopic studies of membrane integral proteins. With a view to using it as a detergent for crystallization trials by the in meso or lipid cubic phase method, it was important to establish the carrying capacity of the cubic mesophase for GDN. This was quantified in the current study using small-angle X-ray scattering for mesophase identification and phase microstructure characterization as a function of temperature and GDN concentration. The data show that the lipid cubic phase formed by hydrated monoolein tolerates GDN to concentrations orders of magnitude in excess of those used for membrane protein studies. Thus, having GDN in a typical membrane protein preparation should not deter use of the in meso method for crystallogenesis.

Using 2-dimensional and 3-dimensional CT scans, Natural History Museum anthropologists found that the brain and major organs were removed and rolls of linen filled out the abdominal cavity. This mummification method is evidence of superior embalming, indicating a person of higher status.

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In 2011, scholars from three institutions—National Museum of American History Curators Carlene Stephens and Shari Stout, Library of Congress Digital Conversion Specialist Peter Alyea and Lawrence Berkeley National Laboratory Scientists Carl Haber and Earl Cornell—came together in a newly designed preservation laboratory at the Library of Congress to recover sound from those recordings made more than 100 years ago.

The post Digital technology allows Alexander Graham Bell’s 1880s disc recordings to be played again appeared first on Smithsonian Insider.

The center of the Milky Way galaxy, with The supermassive black hole Sagittarius A–at center–is revealed in this image. Astronomers have used NASA’s Chandra X-ray Observatory to […]

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Today the Smithsonian launches its Transcription Center website to the public. The website is designed to leverage the power of crowds to help the Smithsonian […]

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Like other kids at summer camp, a group of youngsters in the cities of Miami, Okla. and Fort Wayne, Ind. play games, work on crafts […]

The post Digitized, Searchable Archives Help Revive ‘Sleeping’ Languages appeared first on Smithsonian Insider.

The Smithsonian Research Online program recently surpassed the mark of 10,000 publications in the Digital Repository. This collection of digital publications by Smithsonian staff represents a broad review of research done by researchers at the Institution.

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The Pan-STARRS project at the University of Hawai’i Institute for Astronomy (UH IfA) is publicly releasing the world’s largest digital sky survey today from the […]

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It takes a Tlingit artist up to 2,000 hours, or 83 days, to weave just one ceremonial robe. Not surprisingly, this art form is practiced by a dedicated few including Tlingit artists Teri Rofkar and Shelly Laws of Alaska. In their presentation for the Smithsonian Spotlight series hosted by the Arctic Studies Center at the Anchorage Museum, Rofkar and Laws discuss the methods and cultural significance of robes, spruce root baskets and more.
For more information, go to http://www.mnh.si.edu/arctic/html/alaska.htm

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Given its scale and diversity, the Smithsonian’s collection of 154 million items presents a unique digitization challenge. This video showcases a conveyor belt driven imaging […]

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Millions, if not billions, of specimens reside in the world’s natural history collections, but most of these have not been carefully studied, or even looked […]

The post Using digitized Botany specimens, AI excels in simple curatorial tasks appeared first on Smithsonian Insider.

 Mass Digitization of the living orchid collection maintained by Smithsonian Gardens.

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No green thumb? You don’t need to water these dazzling orchids to enjoy them. More than 8,000 living specimens in the Smithsonian Gardens Orchid Collection […]

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Digital onboarding begins the moment a customer wants to use your products and services and it requires a careful mix of technology and data

(The Paypers) Financial institutions (FIs) working in digital assets have been required by US regulatory bodies to pay attention to their anti-money laundering and...

(The Paypers) Scrive, a Nordic e-sign and eID services provider, and

(The Paypers) Acuant, a global identity verification solutions provider, has teamed up with

(The Paypers) A new survey suggests the majority of Canadians remain worried about the security of their personal information online.

Ingenico Group,

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After 16 years of dedicated planning and engineering, scientists at the U.S. Department of Energy's (DOE) Brookhaven National Laboratory have completed a 3.2 gigapixel sensor array for the camera that will be used in the Large Synoptic Survey Telescope (LSST), a massive telescope that will observe the universe like never before. The digital sensor array is composed of about 200 16-megapixel sensors, divided into 21 modules called "rafts." Each raft can function on its own, but when combined, they will view an area of sky that can fit more than 40 full moons in a single image. Researchers will stitch these images together to create a time-lapse movie of the complete visible universe accessible from Chile. Currently under construction on a mountaintop in Chile, LSST is designed to capture the most complete images of our universe that have ever been achieved. The project to build the telescope facility and camera is a collaborative effort among more than 30 institutions from around the world, and it is primarily funded by DOE's Office of Science and the National Science Foundation.

Image credit: SLAC National Accelerator Laboratory

UAE-based government services ecosystem TAMM has launched the Abu Dhabi Pay platform to...

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome -- commonly referred to as ME/CFS -- is a legitimate, serious, and complex systemic disease that frequently and dramatically limits the activities of affected individuals, says a new report from the Institute of Medicine.

Joint statements from the national science academies of the G7 nations were released today in advance of the G7 Summit to be held in La Malbaie, Canada, on June 8 and 9, 2018.

A new report by research company

Vodafone Egypt has teamed up with TPAY MOBILE, a digital...

Being at your beck and call is central to the "personality" of your digital friend, but there are situations when the device could use some time off

The post Work from home: Should your digital assistant be on or off? appeared first on WeLiveSecurity

The pandemic has highlighted the need for businesses to act with alacrity and prepare for the long haul – and to do so with cybersecurity in mind

The post Digital transformation could be accelerated by COVID‑19 appeared first on WeLiveSecurity

My new book LOSERTHINK, available now on Amazon https://tinyurl.com/rqmjc2a Content: Making the most of our lockdown opportunities The future of the coronavirus task force Testing our way out, versus reality The economy and recovery If you would like to enjoy this same content plus bonus content from Scott Adams, including micro-lessons on lots of useful […]

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Digital noise maps developed under the EU’s Environmental Noise Directive (END) are a useful way of assessing traffic noise exposure for local residents, according to a recent Swedish study. The END maps could also be used to standardise noise exposure information in noise and health research.

Digital initiatives that began pre lockdown are giving the carmarker confidence that it will emerge stronger when the dust settles.

Deployment of AI and cloud technologies is helping the energy firm maintain its operations without disruption.

Genpact sees over 100 clients closing their financial quarter digitally while others are doubling the pace of adoption of digital transformation.

A remote consultation app at Apollo is allowing safety for both the patients and the frontline doctors while using AI to improve diagnostics.

Several of Federal Bank’s digital initiatives are finally seeing massive adoption as the Kochi-based bank works on automation and self service tools to boost profits.

TONIK Financial, Asean’s first licensed digital-only bank, is leveraging cloud to mitigate the risks of Covid-19.

April was a month of heavy-lifting for Anand Natarajan, COO, DBS Bank India, but the company’s investments made towards building a robust tech infrastructure made pandemic substantially easier for him.

Several of Federal Bank’s digital initiatives are finally seeing massive adoption as the Kochi-based bank works on automation and self service tools to boost profits.

In this brave new world, an IP that can help a business quickly adapt to remote working conditions, provide reliable and safe supply chain, and safeguard security, will become the game-changer for businesses to survive and even thrive.

To tackle the Covid-19 situation, Kotak General Insurance is trying to prepare rapidly, test critical paths and scale up

April was a month of heavy-lifting for Anand Natarajan, COO, DBS Bank India, but the company’s investments made towards building a robust tech infrastructure made pandemic substantially easier for him.

Consumers have not been spending on several use-cases online, apart from getting essential services, as the economy takes a hit due to the Covid-19 lockdown.

The tax also applies to advertising revenue earned from companies overseas if those advertisements eventually target customers in India. Google is particularly concerned that it would not be able to swiftly identify countries where such advertising arrangements were in place, Reuters has reported.

The NABH Digital Health Standards aims to consider all relevant aspects of the application of patient interfacing technologies across the continuum of care applicable for outpatient, inpatient, and remote patient monitoring.