This study investigated the effects of long-term soil fertilization on the composition and potential for phosphorus (P) and nitrogen (N) cycling of bacterial communities associated with hyphae of the P-solubilizing fungus Penicillium canescens. Using a baiting approach, hyphosphere bacterial communities were recovered from three soils that had received long-term amendment in the field with mineral or mineral plus organic fertilizers. P. canescens hyphae recruited bacterial communities with a decreased diversity and an increased abundance of Proteobacteria relative to what was observed in soil communities. As core bacterial taxa, Delftia and Pseudomonas spp. were present in all hyphosphere samples irrespective of soil fertilization. However, the type of fertilization showed significant impacts on the diversity, composition, and distinctive taxa/operational taxonomic units (OTUs) of hyphosphere communities. The soil factors P (Olsen method), exchangeable Mg, exchangeable K, and pH were important for shaping soil and hyphosphere bacterial community compositions. An increased relative abundance of organic P metabolism genes was found in hyphosphere communities from soil that had not received P fertilizers, which could indicate P limitation near the fungal hyphae. Additionally, P. canescens hyphae recruited bacterial communities with a higher abundance of N fixation genes than found in soil communities, which might imply a role of hyphosphere communities for fungal N nutrition. Furthermore, the relative abundances of denitrification genes were greater in several hyphosphere communities, indicating an at least partly anoxic microenvironment with a high carbon-to-N ratio around the hyphae. In conclusion, soil fertilization legacy shapes P. canescens hyphosphere microbiomes and their functional potential related to P and N cycling.

IMPORTANCE P-solubilizing Penicillium strains are introduced as biofertilizers to agricultural soils to improve plant P nutrition. Currently, little is known about the ecology of these biofertilizers, including their interactions with other soil microorganisms. This study shows that communities dominated by Betaproteobacteria and Gammaproteobacteria colonize P. canescens hyphae in soil and that the compositions of these communities depend on the soil conditions. The potential of these communities for N and organic P cycling is generally higher than that of soil communities. The high potential for organic P metabolism might complement the ability of the fungus to solubilize inorganic P, and it points to the hyphosphere as a hot spot for P metabolism. Furthermore, the high potential for N fixation could indicate that P. canescens recruits bacteria that are able to improve its N nutrition. Hence, this community study identifies functional groups relevant for the future optimization of next-generation biofertilizer consortia for applications in soil.

A fosfomycin-resistant and carbapenemase (OXA-48)-producing Klebsiella pneumoniae isolate was recovered, and whole-genome sequencing revealed ISEcp1-bla_CTX-M-14b tandemly inserted upstream of the chromosomally encoded lysR-fosA locus. Quantitative evaluation of the expression of lysR and fosA genes showed that this insertion brought a strong hybrid promoter leading to overexpression of the fosA gene, resulting in fosfomycin resistance. This work showed the concomitant acquisition of resistance to broad-spectrum cephalosporins and fosfomycin due to a single genetic event.

Although ample evidence indicates that immune cell homeostasis is an important prognostic outcome determinant in patients with cancer, few studies have examined whether it also determines cancer risk among initially healthy individuals. We performed a case–cohort study including incident cases of breast (n = 207), colorectal (n = 111), lung (n = 70), and prostate (n = 201) cancer as well as a subcohort (n = 465) within the European Prospective Investigation into Cancer and Nutrition-Heidelberg cohort. Relative counts of neutrophils, monocytes, and lymphocyte sublineages were measured by qRT-PCR. HRs and 95% confidence intervals were used to measure the associations between relative counts of immune cell and cancer risks. When relative counts of immune cell types were taken individually, a significant positive association was observed between relative counts of FOXP3+ regulatory T cells (Tregs) and lung cancer risk, and significant inverse associations were observed between relative CD8+ counts and risks of lung and breast cancer (overall and ER+ subtype). Multivariable models with mutual adjustments across immune markers showed further significant positive associations between higher relative FOXP3+ T-cell counts and increased risks of colorectal and breast cancer (overall and ER− subtype). No associations were found between immune cell composition and prostate cancer risk. These results affirm the relevance of elevated FOXP3+ Tregs and lower levels of cytotoxic (CD8+) T cells as risk factors for tumor development.Significance:This epidemiologic study supports a role for both regulatory and cytotoxic T cells in determining cancer risk among healthy individuals.See related commentary by Song and Tworoger, p. 1801

BACKGROUND AND PURPOSE:

Detailed insight into the composition of thrombi retrieved from patients with ischemic stroke by mechanical thrombectomy might improve pathophysiologic understanding and therapy. Thus, this study searched for links between histologic thrombus composition and stroke subtypes and mechanical thrombectomy results.

Obese Europeans who are treated with cholesterol lowering drugs have not only lower values of blood LDL cholesterol and markers of inflammation but in addition a more healthy gut bacteria profile than those obese who are not prescribed statins.

The COVID-19 pandemic has placed unprecedented pressure on societies worldwide, given the pandemic's rapid, often deadly spread. In health care, the pandemic has raised the pressing question of how society should allocate scarce resources during a crisis.

Sierra Leone's president Julius Maada Bio has accused the main opposition party of orchestrating a spate of violent incidents, deepening a political standoff that risks undermining the country's efforts to contain a coronavirus outbreak.

Tara Reade has claimed in a new interview that Joe Biden used graphic language to proposition her for sex when he allegedly assaulted her in 1993.

Read our live coronavirus updates HERE

Teenager's parents criticise Foreign Secretary Dominic Raab's comments on son's death as "misleading"

The first known comet to visit us from another star system has an unusual make-up.

Manchester United legend Roy Keane has offered his support to players like Mesut Ozil, insisting he would have refused to take a pay cut if he found himself in the situation Premier League stars are currently faced with.

Manchester United fans have identified attacking midfield as the position which needs addressing as a priority this summer.

Chelsea fans have identified attack as the position which needs addressing as a priority this summer.

Manchester City fans have called on their club to strengthen in central defence when the transfer window opens.

The 2019/20 season has been a tough one for Tottenham - both on and off the pitch.

Liverpool are enjoying a season few have bettered over the years and should be celebrating their first ever Premier League title.

Ask any fan and Jordan Ayew will rank among their candidates for Crystal Palace player of the season.

Arsenal fans have, somewhat unsurprisingly, identified centre-back as Mikel Arteta's problem position ahead of this summer's transfer window.

Before the suspension of the 2019-20 Premier League campaign, West Ham were embroiled in a fierce fight to retain their top-flight status.

Gladys Berejiklian has again ruled out the prospect of pill testing at music festivals despite a leaked draft coroner's report recommending the move.

Josh Rebholz, who helped with UCLA's fundraising efforts and was a point man on recent coaching hires, is not a finalist for the athletic director job.

Researchers at the Medical University of South Carolina show in a new report that the RNA interference machinery, normally thought to reside in the nucleus or cytoplasm, predominantly localizes to these apical junctions and influences cell biology in the colon. Interestingly, in colon tumors, this localization is dysregulated and may shift the balance of RNAs to promote tumorigenesis.

The modelling predicts more than a quarter of a million jobs could be lost in Victoria due to the coronavirus pandemic in what Premier Daniel Andrews says is the perhaps the "biggest economic and employment challenge" in the state's history.

New Delhi (Sputnik) Apr 22, 2020
Due to the unavailability of a reliable model to predict the electron density of the ionosphere, navigation errors remain, creating technological hurdles. A new model developed by Indian researchers has potential applications in calculating these Global Navigation Satellite System (GNSS) positioning errors. In a major development that could have a wider impact, ranging from accurate aiming

Driving the need for strategic positioning is not a marketing game - it’s a game-changing CEO-level initiative.

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With the recent flurry of changes in Rugby Australia leadership, confusion increases for those involved with a game already in crisis as concerns continue that an expected loan won't be large enough to help.

Attorney General Eric Holder wrote to Senate Majority Leader Harry Reid and Senate Minority Leader Mitch McConnell today in opposition to language in the proposed 2011 Full-Year Continuing Appropriations Act.

"From key federal partners, to policy experts, researchers, and front-line practitioners, each of you is an essential part of the incident response community," said Attorney General Holder.

"Our resilience is due, in great part, to our ability to come together at all levels of government to build and bolster a solid public safety infrastructure," said Assistant Attorney General Robinson.

Ignacia S. Moreno, Assistant Attorney General for the Justice Department’s Environment and Natural Resources Division, today announced two new members of her senior leadership team.

Congress is starting to consider ways to address the budget cap problem that hangs over the entire FY 21 appropriations process for non-defense discretionary (NDD) programs. Last year, Congress broke a long-running stalemate by agreeing to budget caps for FY 20 and FY 21. They decided to front-load the increases, making spending decisions (relatively) easier […]

Rick Bright confirmed as having left his position as director of BARDA as the agency provides aid to develop solutions for COVID-19.

ABOUT CIBR
The Chinese Institute for Brain Research, Beijing (CIBR) was established in March, 2018 by the Beijing Municipal Government and seven institutions in Beijing. Founded to be a pillar of the China Brain Initiative, CIBR aims to support a vibrant interdisciplinary program in neuroscience and its related disciplines, with both basic and applied research focused ultimately on improving human health. CIBR will also take responsibility for coordinating research institutes and managing …

About us

Southeast University (SEU), located at Nanjing, is a prestigious higher education institution with its origin traced back to 1902. As one of the national key universities under direct administration of the Ministry of Education of China and jointly established with Jiangsu Province, it is selected in various programs, such National “Project 211” , “Program 985” and “Class A first-rate world universities” sponsored by the Central Government to build world-class universities.

*M…

About us
University of Shanghai for Science and Technology (USST) emerged from the University of Shanghai founded in 1906 and the German Medical and Engineering School set up in 1907. Carrying on its motto of “Integrity, Righteousness, Diligence, Love, Pondering, Learning, Aspiration, Ambition”, USST aims to cultivate students with knowledge, ambitions, as well as socialist core values. Adhering to the strategy of “developing in partnership with outstanding faculty”, the university now has 16…

Applications are invited for Principal Investigator (PI) positions at IDG/McGovern Institute for Brain Research at PKU. Positions are open at all ranks from Assistant, Associate to Full Investigator ship.
The Institute was established in 2012 with support from Peking University, IDG and Patrick J. McGovern and Lore Harp McGovern, who are committed to improving human welfare, communication and understanding through their support for neuroscience research. The Institute consists of neural and c…

ABOUT CIBR
The Chinese Institute for Brain Research, Beijing (CIBR) was established in March, 2018 by the Beijing Municipal Government and seven institutions in Beijing. Founded to be a pillar of the China Brain Initiative, CIBR aims to support a vibrant interdisciplinary program in neuroscience and its related disciplines, with both basic and applied research focused ultimately on improving human health. CIBR will also take responsibility for coordinating research institutes and managing …

About CNU
Capital Normal University (CNU) was founded in 1954. Being a national “Double-First Class” university and “Province-Ministry Co-sponsored” (Beijing and the Education Ministry) university, CNU has extensively broad disciplines including literature, sciences, engineering, management, law, education, foreign languages and arts. For over 60 years, CNU has cultivated more than 200 thousand graduates specializing in various fields. Currently, CNU is an important talent training base of Be…

About us

Southeast University (SEU), located at Nanjing, is a prestigious higher education institution with its origin traced back to 1902. As one of the national key universities under direct administration of the Ministry of Education of China and jointly established with Jiangsu Province, it is selected in various programs, such National “Project 211” , “Program 985” and “Class A first-rate world universities” sponsored by the Central Government to build world-class universities.

*M…

About us
University of Shanghai for Science and Technology (USST) emerged from the University of Shanghai founded in 1906 and the German Medical and Engineering School set up in 1907. Carrying on its motto of “Integrity, Righteousness, Diligence, Love, Pondering, Learning, Aspiration, Ambition”, USST aims to cultivate students with knowledge, ambitions, as well as socialist core values. Adhering to the strategy of “developing in partnership with outstanding faculty”, the university now has 16…

Applications are invited for Principal Investigator (PI) positions at IDG/McGovern Institute for Brain Research at PKU. Positions are open at all ranks from Assistant, Associate to Full Investigator ship.
The Institute was established in 2012 with support from Peking University, IDG and Patrick J. McGovern and Lore Harp McGovern, who are committed to improving human welfare, communication and understanding through their support for neuroscience research. The Institute consists of neural and c…

With a generous support from the Guangzhou City Government and the Guangdong Provincial Government, Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL) was launched in 2017. GRMH-GDL aims to bring together multiple leading scientific research units from Guangdong, Hong Kong, Macao and well-known international research institutes to achieve the vital goal of "Healthy China" and to cooperatively tackle the scientific and technical problems and challenges. GRMH-GDL is focused…

The HIT Center for Life Sciences (HCLS) was founded in 2016, which is a new strategic development of the university. According to 2020 US News Rankings of Best Global Universities, HIT’s Biology and Biochemistry was ranked 9th in China. As the first special academic zone of Harbin Institute of Technology(HIT), HCLS runs its own graduate program and enjoys unparalleled freedom in research, personnel employment and financial flexibility within the university. 9 Principal Investigators (PIs) star…

The HIT Center for Life Sciences (HCLS) was founded in 2016, which is a new strategic development of the university. According to 2020 US News Rankings of Best Global Universities, HIT’s Biology and Biochemistry was ranked 9th in China. As the first special academic zone of Harbin Institute of Technology(HIT), HCLS runs its own graduate program and enjoys unparalleled freedom in research, personnel employment and financial flexibility within the university. 9 Principal Investigators (PIs) star…