Irrfan Khan, the supremely talented actor bid goodbye to this world today (April 29, 2020). The news of the actor's demise came out as a great shock for both his fans and the industry members. Dulquer Salmaan and Parvathy Thiruvothu, the

A young Malayalam actor, Basil George, who played the lead role in Poovalliyum Kunjadum, died in a road accident near Muvattupuzha. As per the reports, he was among the three killed, and five people were grievously injured. Basil was born to

Priyadarshan, the senior filmmaker is a great admirer of Fahadh Faasil, the National award-winning actor. The director has heaped praises over Fahadh in most of his recent interviews, and has always stated that he is his favourite from the younger generation.

Dedicating a whole day to honour and celebrate the women who raised us is beyond special. Mothers are selfless, they are strong and can overcome any obstacle with all their might to protect their family. With Mother's Day just round the

The news of Irrfan Khan's shocking demise is yet to sink in. The actor died of neuroendocrine tumour on April 29, 2020. One of his films Piku, co-starring Amitabh Bachchan and Deepika Padukone, completes 5 years today (May 8, 2020).

Rocking Star Yash is one of the best dancers in the Kannada film industry. Way back in 2013, the KGF actor had done a special song with Shriya Saran in Roopa Iyer's film, Chandra, which also starred Prem. But the special

Vinay Rajkumar recently unveiled the teaser and posters of his upcoming movies (Gramayana and 10) as birthday gifts to his fans. The actor who turned 31-years-old on May 7, also opened up about the life under lockdown in a recent interview

Kamal Haasan, the senior actor is all praise for Vijay Sethupathi, the supremely talented actor. To the uninitiated, the Ulaganayagan and Makkal Selvan had recently had a live chat on social media. During the chat, Vijay Sethupathi asked Kamal Haasan a

Novel Coronavirus is spreading rapidly across India. The country has so far reported 56,409 COVID-19 positive cases. Due to the Coronavirus outbreak, the government announced a lockdown to curb the spread of the deadly disease. Amidst all, Simbu's fan has recently

Chagas diseases (CD) is a silent yet life-threatening disease caused by a protozoan parasite named Trypanosoma cruzi (T.cruzi). The disease is also known as American trypanosomiasis as it is found mainly in rural areas of America where there is a lack

Thyroid cancer is the cancer of the thyroid gland which is found at the neck's front just below Adam's apple. The thyroid gland is a butterfly-shaped gland with two lobes, one at each side joined by a narrow tissue called the

Thalassemia is a group of inherited blood disorders that has a similar characteristic: defective and reduced production of haemoglobin, the iron-containing protein in red blood cells that helps in carrying oxygen throughout the body. Beta-thalassemia (BT) is one of

Tesla Inc sued local authorities in California on Saturday as the electric carmaker pushed to re-open its factory there and Chief Executive Elon Musk threatened to move Tesla's headquarters and future programs from the state to Texas or Nevada.

He reportedly made the comments during an interview to a Kannada TV channel.

The actress came in support of the artists who were backlashed for their 'intolerance' comment.

Archives, Room Use Only - UG607.U55 1905

McKinnon appears to be on pace for a strong recovery, but 49ers general manager John Lynch is taking a wait-and-see approach.

Work resumes in Greater Noida’s Oppo mobile factory

TIRUVARUR Power tillers and tractors purchased by the Farmers Producers Organisations in Tiruvarur district for deployment in the forthcoming crop sea

‘Management violated all norms’

Parking usage down, contractors seek fee relief

Actor Rajshri Deshpande reaches out to the needy via a crowdfunding platform

The electric car company told employees to report to work Friday, but a county official said Tesla did not meet the requirements for resuming production.

Tesla said the county's position left it no choice but to take legal action to ensure Tesla and its employees can go back to work

A fire broke out in a cardboard factory in outer Delhi’s Bawana on Sunday morning, Delhi Fire Service officials said. However, no one is trapped or in

'Having lived through cancer, through so many ups and downs, I'm not particularly attached to attention or success -- it's lovely if it's there, it's fine if it's not.'

Sushmita Sen won Miss India with a dress selected by a mother, and similarly, Debina played an important role resembling Kajol from Baazigar thanks to her mom

Two in situ `nanoreactors' for high-resolution imaging of catalysts have been designed and applied at the hard X-ray nanoprobe endstation at beamline P06 of the PETRA III synchrotron radiation source. The reactors house samples supported on commercial MEMS chips, and were applied for complementary hard X-ray ptychography (23 nm spatial resolution) and transmission electron microscopy, with additional X-ray fluorescence measurements. The reactors allow pressures of 100 kPa and temperatures of up to 1573 K, offering a wide range of conditions relevant for catalysis. Ptychographic tomography was demonstrated at limited tilting angles of at least ±35° within the reactors and ±65° on the naked sample holders. Two case studies were selected to demonstrate the functionality of the reactors: (i) annealing of hierarchical nanoporous gold up to 923 K under inert He environment and (ii) acquisition of a ptychographic projection series at ±35° of a hierarchically structured macroporous zeolite sample under ambient conditions. The reactors are shown to be a flexible and modular platform for in situ studies in catalysis and materials science which may be adapted for a range of sample and experiment types, opening new characterization pathways in correlative multimodal in situ analysis of functional materials at work. The cells will presently be made available for all interested users of beamline P06 at PETRA III.

The bond-valence method was performed on 51 crystal data sets from nitrogenase proteins, indicating the presence of molybdenum(III) in FeMo cofactors and vanadium(III) with more reduced iron complements in FeV cofactors.

Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases. The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1. As such, recombinant LAP is promising as a protein-based therapeutic for modulating TGFβ-1 activity; however, the mechanism of binding is incompletely understood. Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex shows that LAP is in a more open and extended conformation when unbound to TGFβ-1. Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the `straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold. This conformational change does not appear to include a repositioning of the integrin-binding motif as previously proposed. X-ray scattering-based modelling supports this mechanism and reveals possible orientations and ensembles in solution. Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification. The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.

The Y128F single mutant of p-hydroxymandelate oxidase (Hmo) is capable of oxidizing mandelate to benzoate via a four-electron oxidative decarboxylation reaction. When benzoylformate (the product of the first two-electron oxidation) and hydrogen peroxide (an oxidant) were used as substrates the reaction did not proceed, suggesting that free hydrogen peroxide is not the committed oxidant in the second two-electron oxidation. How the flavin mononucleotide (FMN)-dependent four-electron oxidation reaction takes place remains elusive. Structural and biochemical explorations have shed new light on this issue. 15 high-resolution crystal structures of Hmo and its mutants liganded with or without a substrate reveal that oxidized FMN (FMNox) possesses a previously unknown electrophilic/nucleophilic duality. In the Y128F mutant the active-site perturbation ensemble facilitates the polarization of FMNox to a nucleophilic ylide, which is in a position to act on an α-ketoacid, forming an N5-acyl-FMNred dead-end adduct. In four-electron oxidation, an intramolecular disproportion­ation reaction via an N5-alkanol-FMNred C'α carbanion intermediate may account for the ThDP/PLP/NADPH-independent oxidative decarboxylation reaction. A synthetic 5-deaza-FMNox cofactor in combination with an α-hydroxyamide or α-ketoamide biochemically and structurally supports the proposed mechanism.

Good prior estimates of the effective root-mean-square deviation (r.m.s.d.) between the atomic coordinates of the model and the target optimize the signal in molecular replacement, thereby increasing the success rate in difficult cases. Previous studies using protein structures solved by X-ray crystallography as models showed that optimal error estimates (refined after structure solution) were correlated with the sequence identity between the model and target, and with the number of residues in the model. Here, this work has been extended to find additional correlations between parameters of the model and the target and hence improved prior estimates of the coordinate error. Using a graph database, a curated set of 6030 molecular-replacement calculations using models that had been solved by X-ray crystallography was analysed to consider about 120 model and target parameters. Improved estimates were achieved by replacing the sequence identity with the Gonnet score for sequence similarity, as well as by considering the resolution of the target structure and the MolProbity score of the model. This approach was extended by analysing 12 610 additional molecular-replacement calculations where the model was determined by NMR. The median r.m.s.d. between pairs of models in an ensemble was found to be correlated with the estimated r.m.s.d. to the target. For models solved by NMR, the overall coordinate error estimates were larger than for structures determined by X-ray crystallography, and were more highly correlated with the number of residues.

Most articles dealing with R2TSi3 compounds are only interested in one specific composite or in a series of composites with varying T elements while keeping R fixed (or vice versa). The present work gives an overview of the complete range of 2:1:3 silicides, similar those of Hoffmann & Pöttgen (2001) and Pan et al. (2013). In contrast to the work of Hoffmann & Pöttgen (2001), reasons for formation of the different symmetries and superstructures are discussed. Here, crystallographic properties are in[the] focus, whereas physical and magnetic properties are omitted because those are given by Pan et al. (2013). READS LIKE AN ABSTRACT, please re-write and remove references if possible. Should be two sentences max.

National Zoo lion keeper Rebecca Stites, at right in photo, was joined by Georgie Henley, at left in photo, and Skandar Keynes, actors in the […]

The post Zoo lion cub named “Aslan” by actors Georgie Henley and Skandar Keynes appeared first on Smithsonian Insider.

A local supernova factory has recently started production, according to a wealth of new data from NASA's Chandra X-ray Observatory on the Carina Nebula.

The post The Carina Nebula, a local supernova factory, is ramping up production appeared first on Smithsonian Insider.

With its homely featherless head, undertaker’s charcoal coloring and association with death and decay, the turkey vulture (Cathartes aura) tops few lists as a favorite […]

The post Scientists in awe of huge olfactory bulb found in turkey vulture brain appeared first on Smithsonian Insider.

The bond-valence method has been used for valence calculations of FeMo/V cofactors in FeMo/V proteins using 51 crystallographic data sets of FeMo/V proteins from the Protein Data Bank. The calculations show molybdenum(III) to be present in MoFe7S9C(Cys)(HHis)[R-(H)homocit] (where H4homocit is homocitric acid, HCys is cysteine and HHis is histidine) in FeMo cofactors, while vanadium(III) with a more reduced iron complement is obtained for FeV cofactors. Using an error analysis of the calculated valences, it was found that in FeMo cofactors Fe1, Fe6 and Fe7 can be unambiguously assigned as iron(III), while Fe2, Fe3, Fe4 and Fe5 show different degrees of mixed valences for the individual Fe atoms. For the FeV cofactors in PDB entry 5n6y, Fe4, Fe5 and Fe6 correspond to iron(II), iron(II) and iron(III), respectively, while Fe1, Fe2, Fe3 and Fe7 exhibit strongly mixed valences. Special situations such as CO-bound and selenium-substituted FeMo cofactors and O(N)H-bridged FeV cofactors are also discussed and suggest rearrangement of the electron configuration on the substitution of the bridging S atoms.

Su Ming Sun, Amandine Batte, Mireille Tittel-Elmer, Sophie van der Horst, Tibor van Welsem, Gordon Bean, Trey Ideker, Fred van Leeuwen, and Haico van Attikum

Eukaryotic chromosomes are replicated in interphase and the two newly duplicated sister chromatids are held together by the cohesin complex and several cohesin auxiliary factors. Sister chromatid cohesion is essential for accurate chromosome segregation during mitosis, yet has also been implicated in other processes, including DNA damage repair, transcription and DNA replication. To assess how cohesin and associated factors functionally interconnect and coordinate with other cellular processes, we systematically mapped genetic interactions of 17 cohesin genes centered on quantitative growth measurements of >52,000 gene pairs in budding yeast. Integration of synthetic genetic interactions unveiled a cohesin functional map that constitutes 373 genetic interactions, revealing novel functional connections with post-replication repair, microtubule organization and protein folding. Accordingly, we show that the microtubule-associated protein Irc15 and the prefoldin complex members Gim3, Gim4 and Yke2 are new factors involved in sister chromatid cohesion. Our genetic interaction map thus provides a unique resource for further identification and functional interrogation of cohesin proteins. Since mutations in cohesin proteins have been associated with cohesinopathies and cancer, it may also identify cohesin interactions relevant in disease etiology.

Structural investigations of amorphous and nanocrystalline phases forming in solution are historically challenging. Few methods are capable of in situ atomic structural analysis and rigorous control of the system. A mixed-flow reactor (MFR) is used for total X-ray scattering experiments to examine the short- and long-range structure of phases in situ with pair distribution function (PDF) analysis. The adaptable experimental setup enables data collection for a range of different system chemistries, initial supersaturations and residence times. The age of the sample during analysis is controlled by adjusting the flow rate. Faster rates allow for younger samples to be examined, but if flow is too fast not enough data are acquired to average out excess signal noise. Slower flow rates form older samples, but at very slow speeds particles settle and block flow, clogging the system. Proper background collection and subtraction is critical for data optimization. Overall, this MFR method is an ideal scheme for analyzing the in situ structures of phases that form during crystal growth in solution. As a proof of concept, high-resolution total X-ray scattering data of amorphous and crystalline calcium phosphates and amorphous calcium carbonate were collected for PDF analysis.

Relativistic electron diffraction depends on linear and quadratic terms in the electric potential, the latter being neglected in the frequently used relativistically corrected Schrödinger equation. The quadratic electric potential term modifies atomic scattering amplitudes in particular for large-angle scattering and backscattering. The respective correction increases with increasing scattering angle, increasing atomic number and increasing kinetic energy. Conventional tabulations for electron scattering and its large-angle extrapolations can be amended in closed form by a universal correction based on the screened Coulomb potential squared.

A new aspherical scattering factor formalism has been implemented in the crystallographic least-squares refinement program SHELXL. The formalism relies on Gaussian functions and can optionally complement the independent atom model to take into account the deformation of electron-density distribution due to chemical bonding and lone pairs. Asphericity contributions were derived from the electron density obtained from quantum-chemical density functional theory computations of suitable model compounds that contain particular chemical environments, as defined by the invariom formalism. Thanks to a new algorithm, invariom assignment for refinement in SHELXL is automated. A suitable parameterization for each chemical environment within the new model was achieved by metaheuristics. Figures of merit, precision and accuracy of crystallographic least-squares refinements improve significantly upon using the new model.

An error in an equation in the paper by Song et al. [Acta Cryst. (2019), C75, 1353–1358] is corrected.

Su Ming Sun
Apr 16, 2020; 0:jcs.237628v1-jcs.237628
Articles

Efforts to convert civilian research reactors from weapon-grade highly enriched uranium (HEU) to low enriched uranium (LEU) fuels are taking significantly longer than anticipated, says a congressionally mandated report from the National Academies of Sciences, Engineering, and Medicine.

Our ability to observe and predict severe weather events and other disasters has improved markedly over recent decades, yet this progress does not always translate into similar advances in the systems used in such circumstances to protect lives.

A new report from the National Academies of Sciences, Engineering, and Medicine examines to what extent and in which ways health care utilization -- such as in-patient hospitalizations, emergency department use, and hospital readmission -- reflects disease severity, disability, and ability to perform gainful activity.

Brian Dennehy, known for his interpretations of characters created by Eugene O'Neill and Arthur Miller, has died at the age of 81.; Credit: Mike Piscitelli /Dennehy Family

Brian Dennehy could go from viciously intimidating on film to weak and weary on stage. The actor died yesterday of cardiac arrest. His family says his death was not related to COVID-19. He was 81 years old.

Dennehy had a vast and varied body of work. On screen he was known for his roles in First Blood, Cocoon, and Tommy Boy. In theater, he earned wide acclaim for his depiction of the beleagured Willy Loman in the 1999 Broadway revival of Arthur Miller's Death of a Salesman. That performance earned him one of his two Best Actor Tony Awards. He earned the other in 2003 for his portrayal of family patriarch James Tyrone in Eugene O'Neill's Long Days Journey into Night.

Brian Manion Dennehy was born in Bridgeport, Connecticut, on July 9, 1938, and grew up on Long Island, New York. He was tall and broad-chested and played football in high school, but was also in love with theater. As an Irish Catholic, he didn't see much of a future for himself in acting until he saw Marlon Brando in On the Waterfront. "For the first time when I saw that picture, I realized there were people in the business who looked like me, and who sounded like me. And who came from places I came from" he said in an interview with WHYY's Fresh Air in 1999. "Before that time, acting was like ballet — something I could appreciate but never consider myself a part of."

On stage, Dennehy was a revered actor, particularly in the Chicago theater scene. His two Tony-award winning performances began at the famed Goodman Theatre, where he also performed in Samuel Beckett's Krapp's Last Tape. On Broadway, his credits include Translations, Love Letters, and Inherit the Wind.

Earlier in his career, Dennehy wasn't picky about his roles — and he wasn't shy about it either. "I had kids," he said in the Fresh Air interview. "I had kids who were ready to go to college, and I knew I had the responsibility — which I did not resent — to make sure they had good educations." Dennhey said his wide range of roles on television, where he acted in everything from Dynasty to M*A*S*H to Just Shoot Me, helped him become a more efficient actor. Dennehy worked all throughout his life, most recently appearing in the television series The Blacklist, as well the upcoming independent film Driveways.

This content is from Southern California Public Radio. View the original story at SCPR.org.

New research has investigated the many ways in which technological transfer occurs for wind power projects in developing countries. These range from trading with developed countries, to local innovation. In India and China, successful transfer was found to depend more on existing capabilities in these countries than international projects, such as the Clean Development Mechanism (CDM).