Friedrich Haslinger
Proc. Amer. Math. Soc. 152 (), 5219-5227.
Abstract, references and article information

Jian Li
Proc. Amer. Math. Soc. 152 (), 5207-5217.
Abstract, references and article information

Benjamin Jaye and Manasa N. Vempati
Proc. Amer. Math. Soc. 152 (), 5105-5116.
Abstract, references and article information

T. A. Suslina
St. Petersburg Math. J. 35 (), 537-570.
Abstract, references and article information

Stromal interaction molecule 1 (STIM1) plays a pivotal role in store-operated Ca2+ entry (SOCE), an essential mechanism in cellular calcium signaling and in maintaining cellular calcium balance. Because O-GlcNAcylation plays pivotal roles in various cellular function, we examined the effect of fluctuation in STIM1 O-GlcNAcylation on SOCE activity. We found that both increase and decrease in STIM1 O-GlcNAcylation impaired SOCE activity. To determine the molecular basis, we established STIM1-knockout HEK293 (STIM1-KO-HEK) cells using the CRISPR/Cas9 system and transfected STIM1 WT (STIM1-KO-WT-HEK), S621A (STIM1-KO-S621A-HEK), or T626A (STIM1-KO-T626A-HEK) cells. Using these cells, we examined the possible O-GlcNAcylation sites of STIM1 to determine whether the sites were O-GlcNAcylated. Co-immunoprecipitation analysis revealed that Ser621 and Thr626 were O-GlcNAcylated and that Thr626 was O-GlcNAcylated in the steady state but Ser621 was not. The SOCE activity in STIM1-KO-S621A-HEK and STIM1-KO-T626A-HEK cells was lower than that in STIM1-KO-WT-HEK cells because of reduced phosphorylation at Ser621. Treatment with the O-GlcNAcase inhibitor Thiamet G or O-GlcNAc transferase (OGT) transfection, which increases O-GlcNAcylation, reduced SOCE activity, whereas treatment with the OGT inhibitor ST045849 or siOGT transfection, which decreases O-GlcNAcylation, also reduced SOCE activity. Decrease in SOCE activity due to increase and decrease in O-GlcNAcylation was attributable to reduced phosphorylation at Ser621. These data suggest that both decrease in O-GlcNAcylation at Thr626 and increase in O-GlcNAcylation at Ser621 in STIM1 lead to impairment of SOCE activity through decrease in Ser621 phosphorylation. Targeting STIM1 O-GlcNAcylation could provide a promising treatment option for the related diseases, such as neurodegenerative diseases.

The actin cytoskeleton is of profound importance to cell shape, division, and intracellular force generation. Profilins bind to globular (G-)actin and regulate actin filament formation. Although profilins are well-established actin regulators, the distinct roles of the dominant profilin, profilin 1 (PFN1), versus the less abundant profilin 2 (PFN2) remain enigmatic. In this study, we use interaction proteomics to discover that PFN2 is an interaction partner of the actin N-terminal acetyltransferase NAA80, and further confirm this by analytical ultracentrifugation. Enzyme assays with NAA80 and different profilins demonstrate that PFN2 binding specifically increases the intrinsic catalytic activity of NAA80. NAA80 binds PFN2 through a proline-rich loop, deletion of which abrogates PFN2 binding. Small-angle X-ray scattering shows that NAA80, actin, and PFN2 form a ternary complex and that NAA80 has partly disordered regions in the N-terminus and the proline-rich loop, the latter of which is partly ordered upon PFN2 binding. Furthermore, binding of PFN2 to NAA80 via the proline-rich loop promotes binding between the globular domains of actin and NAA80, and thus acetylation of actin. However, the majority of cellular NAA80 is stably bound to PFN2 and not to actin, and we propose that this complex acetylates G-actin before it is incorporated into filaments. In conclusion, we reveal a functionally specific role of PFN2 as a stable interactor and regulator of the actin N-terminal acetyltransferase NAA80, and establish the modus operandi for NAA80-mediated actin N-terminal acetylation, a modification with a major impact on cytoskeletal dynamics.

2 September 2020

When you log on to the SAS Risk Governance Framework and choose a solution, the web application might fail to load the solution content. When the problem occurs, you continue to see "Loading..." on the screen, an

Nigeria's 2023 elections: Security, economic and foreign policy imperatives 5 December 2022 — 1:00PM TO 2:00PM Anonymous (not verified) 23 November 2022 Chatham House and Online

Bola Ahmed Tinubu, presidential candidate for the All-Progressives Congress, discusses his vision and recently-unveiled manifesto for ‘renewing hope’ in Nigeria.

Nigeria is scheduled to hold presidential and national assembly elections on 25 February 2023 as well as governorship and other subnational elections on 11 March 2023.

The elections will end President Muhammadu Buhari’s two terms in office since his election in 2015 and will mark the first time that he is not engaging in a presidential poll since Nigeria’s transition to civilian rule in 1999 – an important marker in Nigeria’s trajectory of democratic consolidation.

Nigeria’s recently enacted Electoral Act has contributed to improved hope around the election process, reflected in the addition of 12.29 million new voters in Nigeria’s voter registration exercise across the federation’s 36 states and 1,491 constituencies.

Yet Nigeria stands at a critical juncture, having suffered from two recessions in the past six years, unprecedented levels of food insecurity, persistent fuel scarcity and high levels of crude oil theft.

Civic fatigue also remains an important challenge and President Muhammadu Buhari’s three main policy pillars of security, economy and corruption continue to be defining issues for citizens.

At this event, Bola Ahmed Tinubu, presidential candidate for the All-Progressives Congress, discusses his vision and recently unveiled manifesto for ‘renewing hope’ in Nigeria including his policy proposals for economic reform and revival and how to deliver secure and inclusive job opportunities for Nigerian citizens.

Download a transcript

This event is a members and Africa programme event and is part of a series of events and outputs examining Nigeria’s 2023 elections and political developments.

As with all Chatham House member events, questions from members drive the conversation.

Africa and Europe: Cooperation on digital transitions and new technologies 26 May 2022 — 8:00AM TO 12:30PM Anonymous (not verified) 12 May 2022 Online

The 11th Africa Day International Conference takes place under the auspices of the president of the Republic of Slovenia, HE Mr Borut Pahor, and within the framework of the Bled Strategic Forum.

Slovenia’s annual high-level Africa event seeks to improve policy outcomes for citizens in Europe and Africa as a result of a mutual understanding and strengthened cooperation between the two regions.

The event is co-hosted by the Ministry of Foreign Affairs of the Republic of Slovenia, the Chatham House Africa Programme and the European Commission.

Expert discussions at this year’s edition will examine collaborative links between Africa and Europe in promoting responsible innovation and governance of emerging technologies, as well as the role of technology in shaping creative and cultural economies.

The conference will be broadcast live on this website, on the Slovenian Ministry of Foreign Affairs website and on the Africa Programme Facebook page.

Earlier this month, the United States and the Republic of Korea reached an agreement on greater cooperation on civil nuclear energy projects, the U.S. Department of Energy said this week.

Zapata Computing, which was founded in 2017 as a Harvard spinout specializing in quantum software and later pivoted to an AI focus, is ceasing operations, according to an SEC filing […]

The post Zapata Computing, Early Quantum-AI Software Specialist, Ceases Operations appeared first on HPCwire.

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The American Ingenuity Award winner on the connection between history and music

Theater students in Scunthorpe, England, rehearse for their performance celebrating the life of homegrown cyclist Lal White

The world’s slowest mammal is at risk of extinction by the end of the century due to their low metabolic rate and climate change

Sault Ste. Marie has the highest rate of opioid deaths in Ontario so far this year, according to new numbers from the province's coroner's office.

A Winnipeg business owner is suing a man she alleges swindled her out of ownership of two properties after promising financial help — the latest in a string of court proceedings against the man, whom a judge previously described as showing the signs of a "predator" who seeks out financially vulnerable clients.

StandardAero says new funding from the provincial and federal governments will help expand its Winnipeg operations by training more specialized technicians, managers, support personnel and engineers.

CBC Sports' daily newsletter breaks down whose fortunes are looking up and whose are down after the weekend's winter Olympic sports action.

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Pioneering Technology Allows Surgeons to Cut Precisely and Control Bleeding Without Causing Heat Damage to Tissue

Penn State School of Music announces the inaugural performance of its Opera Workshop, led by new faculty members Dawn Pierce and Parker Konkle. The performance, titled "Seasons of Love," will take place on Dec. 10, at 7:30 p.m. in the School of Music’s Recital Hall. This unique presentation will explore a range of lyric repertoire, offering an avant-garde experience that merges music, movement, and storytelling. 

Delhi's air quality remained "very poor" for a 14th consecutive day on Tuesday, with an AQI reading of 334, as vehicular emission was the biggest contributor to the city's pollution, accounting for 15.4 per cent.

A small amount of radioactive debris removed by a robot from Japan's stricken Fukushima nuclear plant has arrived at a research lab near Tokyo, the plant operator said Tuesday, after a journey kept secret for safety reasons.

Delhi's air quality remained "very poor" for a 14th consecutive day on Tuesday, with an AQI reading of 334, as vehicular emission was the biggest contributor to the city's pollution, accounting for 15.4 per cent.

Team India is set to feature in an intra-squad warm-up game, starting Friday. But, fans won't be able to see the players in action.

Delays impacting operations, including payments and insurance transactions Earlier this month, one of the nation’s largest health care companies experienced a cybersecurity attack immobilizing much of the industry. Change Healthcare, a subsidiary of UnitedHealth Group that also operates as Optum Solutions, states that it interacts with 1 in every 3 patient records. Their systems are […]

NDTV Profit, the business news channel of the New Delhi Television Ltd, is set to resume regular operations from December.

Defendants placed more than 1.3 billion deceptive fundraising calls claiming to support veterans, children, firefighters The Delaware Department of Justice, along with the Federal Trade Commission and 46 agencies from 38 states and the District of Columbia, has stopped a massive telefunding operation that bombarded 67 million consumers with 1.3 billion deceptive charitable fundraising calls, […]

Defendants sentenced to total of 47 years for gun, drug offenses Attorney General Kathy Jennings announced Friday that the Department of Justice secured drug and firearms sentences in February against three Kent County men who were arrested in connection with the joint state/federal Operation Rise-N-Shyne investigation. “Justice called for robust sentences in these cases,” said […]

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DOVER (Dec. 10, 2021) – On Thursday, Dec. 9,  2021, the Delaware Division of Public Health (DPH) ordered a Millsboro man to cease tattooing activities in his home, in violation of Title 16 of the Delaware Code. Body art activities should only be performed in DPH-permitted facilities. Private residences are not properly equipped for these activities to […]

NEW CASTLE (Julio 12, 2022) – El Departamento de Salud y Servicios Sociales (DHSS) anunció hoy que ha seleccionado tres compañias para operar su Programa de Atención Administrada de Medicaid. La División de Medicaid y Asistencia Médica (DMMA) del DHSS continuará su asociación de MCO con Highmark Health Options Blue Cross Blue Shield, que empezó […]

NEW CASTLE (July 12, 2022) – The Department of Health and Social Services (DHSS) announced today that it has selected three companies to operate its Medicaid Managed Care Program. DHSS’ Division of Medicaid and Medical Assistance (DMMA) will continue its MCO partnership with Highmark Health Options Blue Cross Blue Shield, which began in 2015, and […]

Due to a reduction in the Air Quality Index (AQI) CASH BOOTHS at the toll plazas OPENED at 4:45 pm today, June 8, 2023. The Delaware Division of Motor Vehicles (DMV) will continue to monitor the AQI forecast and determine the effects it may have, if any, on DMV operations for Friday, June 9, 2023. Residents […]

Attorney General Kathy Jennings today joined a coalition of 50 states and territories announcing two significant cooperation agreements and settlements with Heritage Pharmaceuticals and Apotex totaling $49.1 million to resolve allegations that both companies engaged in widespread, long-running conspiracies to artificially inflate and manipulate prices, reduce competition, and unreasonably restrain trade with regard to numerous generic prescription […]

DEPARTMENT OF STATE: Office of the Alcoholic Beverage Control Commissioner