The renewal deadline is May 15 with 5% down to start. Refunds will be provided for unplayed games

The International Monetary Fund has approved a $226 million loan to help Cameroon to combat the coronavirus as it faced plunging oil revenues.

The following article, Fed Judge Releases 21-Yr-Old Man To “Clean and Sober House” After Appearing In Court For Sexually Assaulting 12-Yr-Old Girl For One Month While Hiding In Her Bedroom, was first published on 100PercentFedUp.com.

The normalizing of pedophilia is not a far-right conspiracy theory...

Continue reading: Fed Judge Releases 21-Yr-Old Man To “Clean and Sober House” After Appearing In Court For Sexually Assaulting 12-Yr-Old Girl For One Month While Hiding In Her Bedroom ...

The CRISPR/Cas9 nucleases have been widely applied for genome editing in various organisms. Cas9 nucleases complexed with a guide RNA (Cas9–gRNA) find their targets by scanning and interrogating the genomic DNA for sequences complementary to the gRNA. Recognition of the DNA target sequence requires a short protospacer adjacent motif (PAM) located outside this sequence. Given that the efficiency of target location may depend on the strength of interactions that promote target recognition, here we sought to compare affinities of different Cas9 nucleases for their cognate PAM sequences. To this end, we measured affinities of Cas9 nucleases from Streptococcus pyogenes, Staphylococcus aureus, and Francisella novicida complexed with guide RNAs (gRNAs) (SpCas9–gRNA, SaCas9–gRNA, and FnCas9–gRNA, respectively) and of three engineered SpCas9–gRNA variants with altered PAM specificities for short, PAM-containing DNA probes. We used a “beacon” assay that measures the relative affinities of DNA probes by determining their ability to competitively affect the rate of Cas9–gRNA binding to fluorescently labeled target DNA derivatives called “Cas9 beacons.” We observed significant differences in the affinities for cognate PAM sequences among the studied Cas9 enzymes. The relative affinities of SpCas9–gRNA and its engineered variants for canonical and suboptimal PAMs correlated with previous findings on the efficiency of these PAM sequences in genome editing. These findings suggest that high affinity of a Cas9 nuclease for its cognate PAM promotes higher genome-editing efficiency.

The CRISPR/Cas9 nucleases have been widely applied for genome editing in various organisms. Cas9 nucleases complexed with a guide RNA (Cas9–gRNA) find their targets by scanning and interrogating the genomic DNA for sequences complementary to the gRNA. Recognition of the DNA target sequence requires a short protospacer adjacent motif (PAM) located outside this sequence. Given that the efficiency of target location may depend on the strength of interactions that promote target recognition, here we sought to compare affinities of different Cas9 nucleases for their cognate PAM sequences. To this end, we measured affinities of Cas9 nucleases from Streptococcus pyogenes, Staphylococcus aureus, and Francisella novicida complexed with guide RNAs (gRNAs) (SpCas9–gRNA, SaCas9–gRNA, and FnCas9–gRNA, respectively) and of three engineered SpCas9–gRNA variants with altered PAM specificities for short, PAM-containing DNA probes. We used a “beacon” assay that measures the relative affinities of DNA probes by determining their ability to competitively affect the rate of Cas9–gRNA binding to fluorescently labeled target DNA derivatives called “Cas9 beacons.” We observed significant differences in the affinities for cognate PAM sequences among the studied Cas9 enzymes. The relative affinities of SpCas9–gRNA and its engineered variants for canonical and suboptimal PAMs correlated with previous findings on the efficiency of these PAM sequences in genome editing. These findings suggest that high affinity of a Cas9 nuclease for its cognate PAM promotes higher genome-editing efficiency.

Telehealth and GP service Babylon has rolled out a "COVID-19 Care Assistant" with a raft of features designed to help subscribers cope with symptoms during the pandemic, from the safety of their phone screen

(Association for Computing Machinery) Today, the ACM Europe Technology Policy Committee (Europe TPC) of the world's largest society of computing professionals, the Association for Computing Machinery (ACM), has released detailed principles and practices for the development and deployment of 'contact tracing' technology intended to track and arrest the spread of COVID-19.

Known for her creative dance moves and twerking style, dancer-turned-artiste Daniiboo dropped a new twerking song, titled ' Drop It N Pop It', in an attempt to put a smile on her fans' faces in a time of unprecedented uncertainty. The song is...

Gather round as three men spend 30 minutes talking about tech. Wait, what do you mean 'no thanks'?! Matt Egan shares the mic with David Price and Henry Burrell to thrash out why Apple deemed a press release adequate to announce the successor to 2014's iPad Air 2. There's also a slick new red iPhone, and a black OnePlus 3T. What's with the limited editions, eh? Or are they even limited? The gang also chat about the developer preview of Android O, out now while Android N is barely months old. Is it too early to say if it'll kick ass, and why do Google and Apple need such long public lead time with new OS builds?  

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The NFL revealed its 2020 regular-season schedule Thursday, with the Kansas City Chiefs kicking off their Super Bowl title defense against the Houston Texans in Week 1.

The Pentagon announced Tuesday that Sgt. Christopher Wesley Curry died Monday in Iraq in what officials describe as a non-combat-related incident.

The Department of Health and Human Services released Nov. 25 a five-minute video explaining how to search its list of excluded individuals and entities, called LEIE.

The handout covers strategies for helping prevent the transmission of suspected respiratory disease in the dental health care setting and answers frequently asked questions related to the virus, based on guidelines from the Centers for Disease Control and Prevention.

Washington — The Environmental Protection Agency announced March 13 that it was adding nearly 200 additional products that have qualified for use against SARS-CoV-2, the novel coronavirus that causes the disease COVID-19.

The U.S. Department of Labor Wage and Hour Division released new guidance March 26 to help workers and employers understand provisions included in the Families First Coronavirus Response Act.

The American Dental Association has released interim guidance for dentists on how to minimize the risk of COVID-19 transmission before, during and after treating dental emergencies.

The Department of Labor released an FAQ April 27 that addresses common questions about work search and job refusal when an individual receives Pandemic Unemployment Assistance.

The ADA Health Policy Institute held a webinar April 29 with leading experts from across the dental industry to discuss how COVID-19 has affected the dental economy and what they think the future will bring.

As the novel coronavirus disease COVID-19 continues to spread, questions have been raised about the potential impact on food supply and availability and on livelihoods around the globe.

FAO

Following the release of the fourth betas of iOS 13.5 and iPadOS 13.5, Apple has followed through with fourth betas for macOS 10.15.5, watchOS 6.2.5, and tvOS 13.4.5.

PRISON governors have been handed the power to veto prisoners they have concerns over being released early - as plans are pushed forward for up to 450 prisoners to be freed to allow inmates to socially distance in cells.

Canon is lifting the lid on the tech inside its upcoming EOS-1D X Mark III SLR. It's a fully pro, and fully loaded camera, with 20fps Raw imaging and 5.5K Raw video capture.

To help teachers remotely engage their students during the coronavirus crisis, Penn State’s Holocaust, Genocide and Human Rights Education Initiative has released its first set of free learning resources.

Governor Jack Markell today joined Delaware State Housing Authority (DSHA) for the release of the Delaware Housing Needs Assessment 2015-2020. The report, prepared by GCR Incorporated of New Orleans, Louisiana and The Reinvestment Fund (TRF) of Philadelphia, Pennsylvania, will drive planning for housing resources at DSHA and in communities around the State. With this new analysis of housing needs and market conditions in the state for years 2015-2020, DSHA will be identifying priorities for the next five years and evaluating its programs and strategies.

Recommendations include expanded residential treatment, public education, and naloxone access The Delaware Drug Overdose Fatality Review Commission (DOFRC) has released its first annual report to Governor John Carney and the General Assembly with recommendations aimed at reducing overdose deaths in Delaware based on review of the circumstances of deaths over the past year. The 23-page […]

Newport, Del.– Discovering Delaware went digital to celebrate National American Beer Day as the Delaware Tourism Office (Visit Delaware) unveiled Delaware on Tap, the new smartphone application version of the state’s popular Beer, Wine & Spirits Trail. The office showcased the app, which is available for free download in the App Store and from Google […]

Working to preserve Delaware’s heritage for the future, the plan outlines six broad goals for the next five years.

Policy will allow state to consistently address discrimination in the workplace WILMINGTON, Del. – Governor Carney on Monday released a uniform, anti-discrimination policy that will allow state agencies to consistently respond to reports of discrimination, harassment and retaliation in the workplace – and clearly outlines a consistent policy for state employees to report and resolve […]

Board presents recommendations across focus areas to improve efficiency and accountability in state government WILMINGTON, Del. – Governor John Carney on Thursday released the second annual report of the Government Efficiency and Accountability Review (GEAR) Board. Governor Carney established GEAR by Executive Order in February 2017 to identify ways for state government to operate more […]

NEW CASTLE (May 30, 2018) – As a way to better support the needs of children with medical complexity and their families, the Division of Medicaid and Medical Assistance this week released Delaware’s Plan for Managing the Health Care Needs of Children with Medical Complexity and announced that stakeholders will continue work to develop longer-term […]

Cancer screening and early detection efforts continue to drive down Delaware’s all-site cancer mortality rates, say Delaware public health officials. From 2000-2004 to 2010-2014, Delaware’s cancer death rate decreased 12 percent, an impressive improvement though slightly lower than the decline seen nationally (14 percent), according to the latest cancer data announced by the Division of Public Health (DPH).

NEW CASTLE (July 24, 2018) – Following a 14-month review of Delaware’s opioid use disorder treatment system, a research team from the Johns Hopkins Bloomberg School of Public Health and the Bloomberg American Health Initiative today recommended four major strategies to achieve the state’s goal of a system of care that is accessible, evidence-based, individualized, […]

Delaware one of 11 participating states vetting strategies around crises such as Hepatitis C and opioid use disorder WILMINGTON, Del. — Delaware was one of 11 states that vetted potential strategies through the National Governors Association (NGA) Center for Best Practices to address public health crises such as Hepatitis C and opioid use disorder by […]

NEW CASTLE (Jan. 2, 2019) – The number of full-time equivalent primary care physicians providing direct patient care in Delaware in 2018 declined about 6 percent from 2013, a trend that resulted in a slightly lower percentage of physicians statewide who are accepting new patients, according to a new University of Delaware study of the […]

DOVER – Cancer screening and early detection efforts continue to drive down Delaware’s cancer death rates, say state public health officials. In particular, officials are seeing dramatic improvements in the all-site mortality rate among non-Hispanic African-American men, which decreased by 30 percent between the five-year periods of 2001-2005 and 2011-2015, according to the latest cancer […]

WILMINGTON  – For the first time, the Delaware Division of Public Health (DPH) through the integration of 12 multi-agency datasets, has developed a demographic picture of the Delawareans who died from drug overdoses in 2017. DPH released the Drug Overdose Mortality Surveillance Report, Delaware, 2017, in Wilmington on Wednesday, Aug. 14, 2019. In addition to […]

The Coronavirus Aid, Relief, and Economic Security (CARES) Act recently passed by the United States Congress and signed into law on March 27th provides unemployment assistance to independent contractors and those self-employed who have been affected by the Coronavirus.

The federal Coronavirus Aid, Relief, and Economic Stability Act (CARES Act) created a new program called Pandemic Emergency Unemployment Compensation (PEUC), which provides 13 weeks of extended unemployment benefits to eligible workers.

Governor announces rolling reopening; State of Emergency extended through May 31 WILMINGTON, Del. – In coordination with public health experts at the Delaware Division of Public Health (DPH), Governor John Carney on Friday announced June 1 as the target date for Phase I of Delaware’s economic reopening. Governor Carney has extended Delaware’s State of Emergency declaration, […]

Texas salon owner Shelley Luther has been ordered released from jail after the governor and attorney general protested her imprisonment over violating the state’s lockdown and modified the shutdown orders to exclude imprisonment.
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The Centre has released Rs 11,092 crore as the first instalment in FY21 to State Disaster Response Mitigation Fund of 28 states.