In May 2024, CC organized a strategic workshop in Lisbon to develop a roadmap for future action to advance our work towards a UNESCO instrument on open cultural heritage. In this blog post, we share the full report and some of its key highlights.

The post CC strategic workshop reveals big opportunities for open access to cultural heritage appeared first on Creative Commons.

Creative Commons (CC) is proud to launch the TAROCH Coalition (Towards a Recommendation on Open Cultural Heritage), a collaborative effort to achieve the adoption of a UNESCO standard-setting instrument to improve open access to cultural heritage. We are grateful to the Arcadia Fund for supporting this initiative. Below we share an overview of TAROCH and…

The post Creative Commons Launches TAROCH Coalition for Open Access to Cultural Heritage appeared first on Creative Commons.

Shopping for clothing is one of my favorite past times — and with all the early Black Friday apparel sales happening now, it's starting to feel like Christmas morning! Apparel and shoes are seeing some of the biggest markdowns with popular brands like Lululemon, Nike, Adidas, Patagonia and more…

European stock indexes traded lower in Wednesday's premarket session, with the US Consumer Price Index (CPI) report firmly on investors' radar ahead of its release at 2:30 pm CET. Analysts anticipate ...

European stock indexes traded lower in Tuesday's premarket session, with the US Consumer Price Index (CPI) report firmly on investors' radar ahead of its release at 2:30 pm CET. Analysts anticipate in...

Making use of the Hashtag for Twitter You probably already have a twitter account up and running for your business. If not, you may want to consider one now. Twitter is a great platform for sharing links to your site and it’s also a great way to connect with your customers on a more personal […]

One of the oldest conspiracy and 'black helicopter' frauds is now facing the hand of the law. Alex Jones is reaping what he sowed.

During a portion of his Info Wars program, Alex Jones lamented over how his show is being stripped and sold off after a just verdict against him for his lies about Sandy Hook victims and their families.

A verdict he described as a "Nazi show trial."

Sucks to be you, Alex. And it couldn't happen to a more deserving guy.

JONES: InfoWars, the equipment, InfoWars.com, InfoWarsStore.com, and a whole bunch of other stuff is at a federal bankruptcy auction from the fake judgments and the rigged trials where I was found guilty beforehand and they had literal show trials out of the Soviet Union or Nazi Germany.

Robin, I walked in here during the break after getting a glass of water, saw the auctioneers inside the building, going around surveying from the last time they were here to make sure all the stuff's here. Everything tagged, everything marked, this death.

Good riddance, you motherfrakker.

Rudy Giuliani is trying to avoid the same fate.

We need more of these creeps to face the same punishment.

read more

With a current zeitgeist around environmental protections and sustainability, more homeowners than ever are focused on green design and eco-friendly features. Add in the benefit of energy and water savings and it’s easy to see why green homes are in high demand. But all is not equal with sustainable architecture from one area of the country to another. As a report sponsored by Payless Power shows, there’s a wide variation in the value of a green home, meaning if the goal is to benefit the environment and your pocketbook you may want to consider the location of your new abode. [...]

The National Science Foundation has awarded a $52.66 million grant to fund a major infrastructure upgrade to the Simons Observatory in the Atacama Desert of Chile. Upgrades are expected to take about five years; the resulting facility will be known as the Advanced Simons Observatory (ASO).

William Dudley, senior advisor, Griswold Center for Economic Policy Studies, Princeton University; and former president of the Federal Reserve Bank of New York In conversation with Markus Brunnermeier, Edwards S. Sanford Professor of Economics, and Director of the Bendheim Center for Finance, Princeton University Wednesday, November 20, 2024, from 4:30-5:30 p.m. Julis Romo Rabinowitz Building, Room 399 Co-sponsored by The Griswold Center for Economic Policy Studies (GCEPS) and Bendheim Center for Finance (BCF) Open to the Princeton University Community Group of 30 Report Publication, G30 Working Group on the 2023 Banking Crisis, chaired by William Dudley

  You can hardly find a person who doesn’t know what Instagram is. And you can barely find internet marketers who don’t use Instagram in their marketing campaigns. Instagram is very popular and some even think that it will overtake Facebook. Actually, Facebook and Instagram are now closely collaborated, which only strengthens both of them.  […]

The post Why Is Instagram Content So Important? appeared first on RSSground.com.

We are glad to announce a new tool added to RSS Ground collection - Instagram Feeds.

The post New Instagram Feeds Generator! appeared first on RSSground.com.

Now you can specify a pool of hashtags, and random hashtags from your pool will be added to your posts.

The post Advanced Hashtags Feature Will Enhance Your Automated Posting appeared first on RSSground.com.

  We are glad to introduce our brand new automated Instagram Poster!  Instagram is a very popular social network that allows you to attract more visitors and customers with your marketing campaigns. You can now send images from your favorite content feeds to your Instagram accounts. Read: Instagram Poster user guidePlease note, that you are able to […]

The post Meet Brand New Instagram Poster appeared first on RSSground.com.

How pheromones and emotional contagion can make fear spread through a group–for better or worse.

7-minute video shows you how to filter and display data in a datagrid as part of your digital signage software presentations for display on any screen, anywhere. This feature is available in Repeat Signage Corporate or Media Wall editions and is ideal for corporate offices to help keep staff updated on relevant information

Photographer Zion Ozeri, whose parents immigrated to Israel from Yemen, documents the last generation of a bygone era in The Jews of Yemen – The Last Generation.

Virgin Atlantic and British Airways have announced further suspension of routes

40% of AI data centers will face operational constraints due to power shortages by 2027 as AI drives unprecedented energy consumption, research firm Gartner said on Tuesday. Data center power requirements for AI-optimized servers are projected to reach 500 terawatt-hours annually by 2027, more than double 2023 levels, as companies rapidly expand facilities to handle large language model training and implementation. The surge in power demand will outpace utility providers' ability to expand capacity, Gartner analyst Bob Johnson said, leading to higher electricity costs that will cascade through the AI industry. Some operators are already seeking direct agreements with power producers to secure guaranteed supply.

Read more of this story at Slashdot.

Sinwar, who topped Israel's most-wanted list, was a key figure believed to have been behind the 7 October attacks.

The BBC's analysis editor Ros Atkins looks at footage said to show Yahya Sinwar's final moments.

With her eye in the sky, Joanna Steidle captures images of the elusive creatures.

Police bodycam footage of Trump 2020 campaign Advisor Brad Parscale’s arrest was released on Monday showing officers bodyslamming Parscale once he emerged outside his home shirtless with a beer in his hand during a tense scene after his wife told the police that he was suicidal. “Listen, I’m not trying to kill myself. She’s lying, I didn’t […]

The post Police Bodycam Footage Released Shows Trump 2020 Adviser Brad Parscale Bodyslammed During Arrest appeared first on Hispolitica.

Generative AI Is Your Ally for Smart Staging, Selling

How Far Is Too Far With Home Staging?

Meta Platforms is rolling out enhanced privacy and parental controls for Instagram accounts of users under 18 in a significant overhaul aimed at addressing growing concerns around the negative effects of social media.

Meta launched AI chatbots voiced by Hollywood celebrities including Judi Dench and John Cena on Wednesday, betting its billions of users are eager to embrace artificial intelligence.

Update: Surveillance footage of the shooting shown in court

Letcher County Sheriff Shawn "Mickey" Stines shot dead District Judge Kevin Mullins in his chambers, was charged with first-degree murder, and has pleaded not guilty. It soon became apparent that Stines had no intention of resigning, despite the inconvenience of his incarceration, leaving authorities to deal with the long and difficult process of removing him from office. — Read the rest

The post Kentucky Sheriff who killed judge plans to retire; footage of shooting shown in court appeared first on Boing Boing.

Surveillance footage of Sheriff Shawn "Mickey" Stines shooting and killing District Judge Kevin Mullins in his own chambers was played in a Kentucky court yesterday, shocking onlookers. Judge Rupert Wilhoit found probable cause to refer Stines' first-degree murder charge to a grand jury. — Read the rest

The post Surveillance footage of Kentucky Sheriff killing judge shown in court appeared first on Boing Boing.

The following article, INTERNal Stupidity: Pentagon Interns Post Pic of Chinese Plane on Veteran’s Day, was first published on Conservative Firing Line.

A knock-off Chinese plane similar to the US F-35 was inadvertently posted by the F-35 JPO (Joint Program Office) on Veteran’s Day. Somebody needs to help this administration figure out some basics …oh wait, a new administration is coming in January. Good. The JPO Strike Force account had no clue what they did until an …

Continue reading INTERNal Stupidity: Pentagon Interns Post Pic of Chinese Plane on Veteran’s Day ...

There appeared to be a definite frost between first lady Jill Biden and Vice President Kamala Harris during a Veterans Day event at Arlington National Cemetery on Monday. A close-up […]

The post Footage of Jill Biden and Kamala Harris Sitting Side-by-Side at Veterans Day Event Goes Viral appeared first on The Western Journal.

Left-wing networks had plenty of bad news for their viewers as former President Donald Trump stormed to victory in the Nov. 5 election. Now, they’re getting plenty of bad news […]

The post Insult to Injury: MSNBC and CNN Suffer Staggering Ratings Plunge Following Trump Victory appeared first on The Western Journal.

Legendary Christian writer C.S. Lewis gave us an analogy to help explain otherwise inexplicable moments such as this. Tolerance, Lewis once wrote, “parodies love as flippancy parodies merriment.” In a […]

The post Biden Asked If He'll Get a Hostage Deal by the End of His Term, and His Response Is Telling appeared first on The Western Journal.

The rare feat of winning both the Two Oceans and Soweto marathons has marked Onalenna Khonkhobe’s breakthrough season, establishing him as one of South Africa’s rising road-running stars.

Christian Tagwerker
Apr 1, 2006; 5:737-748
Research

Eiji Kinoshita
Apr 1, 2006; 5:749-757
Technology

Philip L. Ross
Dec 1, 2004; 3:1154-1169
Research

Expert

TNF is a highly pro-inflammatory cytokine that contributes not only to the regulation of immune responses but also to the development of severe inflammatory diseases. TNF is synthesized as a transmembrane protein, which is further matured via proteolytic cleavage by metalloproteases such as ADAM17, a process known as shedding. At present, TNF is mainly detected by measuring the precursor or the mature cytokine of bulk cell populations by techniques such as ELISA or immunoblotting. However, these methods do not provide information on the exact timing and extent of TNF cleavage at single-cell resolution and they do not allow the live visualization of shedding events. Here, we generated C-tag TNF as a genetically encoded reporter to study TNF shedding at the single-cell level. The functionality of the C-tag TNF reporter is based on the exposure of a cryptic epitope on the C terminus of the transmembrane portion of pro-TNF on cleavage. In both denatured and nondenatured samples, this epitope can be detected by a nanobody in a highly sensitive and specific manner only upon TNF shedding. As such, C-tag TNF can successfully be used for the detection of TNF cleavage in flow cytometry and live-cell imaging applications. We furthermore demonstrate its applicability in a forward genetic screen geared toward the identification of genetic regulators of TNF maturation. In summary, the C-tag TNF reporter can be employed to gain novel insights into the complex regulation of ADAM-dependent TNF shedding.

Visual Abstract

Felicia Grasso
Dec 1, 2020; 19:1986-1996
Research

Juntuo Zhou
Nov 30, 2020; 0:RA120.002384v1-mcp.RA120.002384
Research

Titles and footnotes do not span the entire width of the page when you use the COLUMNS= option with a value that is greater than 1 with the TAGSETS.RTF_SAMPLE tagset. When a value that is greater than 1 is specified for th

Coronavirus disease 2019 (COVID-19) is a highly contagious infection and threating the human lives in the world. The elevation of cytokines in blood is crucial to induce cytokine storm and immunosuppression in the transition of severity in COVID-19 patients. However, the comprehensive changes of serum proteins in COVID-19 patients throughout the SARS-CoV-2 infection is unknown. In this work, we developed a high-density antibody microarray and performed an in-depth proteomics analysis of serum samples collected from early COVID-19 (n = 15) and influenza (n = 13) patients. We identified a large set of differentially expressed proteins (n = 132) that participate in a landscape of inflammation and immune signaling related to the SARS-CoV-2 infection. Furthermore, the significant correlations of neutrophil and lymphocyte with the CCL2 and CXCL10 mediated cytokine signaling pathways was identified. These information are valuable for the understanding of COVID-19 pathogenesis, identification of biomarkers and development of the optimal anti-inflammation therapy.

Plasmodium, the malaria parasite, undergoes a complex life cycle alternating between a vertebrate host and a mosquito vector of the genus Anopheles. In red blood cells of the vertebrate host, Plasmodium multiplies asexually or differentiates into gamete precursors, the male and female gametocytes, responsible for parasite transmission. Sexual stage maturation occurs in the midgut of the mosquito vector, where male and female gametes egress from the host erythrocytes to fuse and form a zygote. Gamete egress entails the successive rupture of two membranes surrounding the parasite, the parasitophorous vacuole membrane and the erythrocyte plasma membrane. In this study, we used the rodent model parasite Plasmodium berghei to design a label-free quantitative proteomic approach aimed at identifying gender-related proteins differentially released/secreted by purified mature gametocytes when activated to form gametes. We compared the abundance of molecules secreted by wild type gametocytes of both genders with that of a transgenic line defective in male gamete maturation and egress. This enabled us to provide a comprehensive data set of egress-related molecules and their gender specificity. Using specific antibodies, we validated eleven candidate molecules, predicted as either gender-specific or common to both male and female gametocytes. All of them localize to punctuate, vesicle-like structures that relocate to cell periphery upon activation, but only three of them localize to the gametocyte-specific secretory vesicles named osmiophilic bodies. Our results confirm that the egress process involves a tightly coordinated secretory apparatus that includes different types of vesicles and may put the basis for functional studies aimed at designing novel transmission-blocking molecules.

The choice for adjuvant chemotherapy in stage II colorectal cancer (CRC) is controversial as many patients are cured by surgery alone and it is difficult to identify patients with high-risk of recurrence of the disease. There is a need for better stratification of this group of patients. Mass spectrometry imaging could identify patients at risk. We report here the N-glycosylation signatures of the different cell populations in a group of stage II CRC tissue samples. The cancer cells, compared to normal epithelial cells, have increased levels of sialylation and high-mannose glycans, as well as decreased levels of fucosylation and highly branched N-glycans. When looking at the interface between cancer and its microenvironment, it seems that the cancer N-glycosylation signature spreads into the surrounding stroma at the invasive front of the tumor. This finding was more outspoken in patients with a worse outcome within this sample group.

Paramphistomosis, caused by the rumen fluke, Calicophoron daubneyi, is a parasitic infection of ruminant livestock which has seen a rapid rise in prevalence throughout Western Europe in recent years. Following ingestion of metacercariae (parasite cysts) by the mammalian host, newly-excysted juveniles (NEJs) emerge and invade the duodenal submucosa which causes significant pathology in heavy infections. The immature larvae then migrate upwards, along the gastrointestinal tract, and enter the rumen where they mature and begin to produce eggs. Despite their emergence, and sporadic outbreaks of acute disease, we know little about the molecular mechanisms used by C. daubneyi to establish infection, acquire nutrients and to avoid the host immune response. Here, transcriptome analysis of four intra-mammalian life-cycle stages, integrated with secretome analysis of the NEJ and adult parasites (responsible for acute and chronic disease respectively), revealed how the expression and secretion of selected families of virulence factors and immunomodulators are regulated in accordance with fluke development and migration. Our data show that whilst a family of cathepsins B with varying S2 sub-site residues (indicating distinct substrate specificities) are differentially secreted by NEJs and adult flukes, cathepsins L and F are secreted in low abundance by NEJs only. We found that C. daubneyi has an expanded family of aspartic peptidases, which is up-regulated in adult worms, although they are underrepresented in the secretome. The most abundant proteins in adult fluke secretions were helminth defence molecules (HDMs) that likely establish an immune environment permissive to fluke survival and/or neutralise pathogen-associated molecular patterns (PAMPs) such as bacterial lipopolysaccharide in the microbiome-rich rumen. The distinct collection of molecules secreted by C. daubneyi allowed the development of the first coproantigen-based ELISA for paramphistomosis which, importantly, did not recognise antigens from other helminths commonly found as co-infections with rumen fluke.

The histopathological subtype of lung adenocarcinoma (LUAD) is closely associated with prognosis. Micropapillary or solid predominant LUAD tends to relapse after surgery at an early stage, whereas lepidic pattern shows a favorable outcome. However, the molecular mechanism underlying this phenomenon remains unknown. Here, we recruited 31 lepidic predominant LUADs (LR: low-risk subtype group) and 28 micropapillary or solid predominant LUADs (HR: high-risk subtype group). Tissues of these cases were obtained and label-free quantitative proteomic and bioinformatic analyses were performed. Additionally, prognostic impact of targeted proteins was validated using The Cancer Genome Atlas databases (n=492) and tissue microarrays composed of early-stage LUADs (n=228). A total of 192 differentially expressed proteins were identified between tumor tissues of LR and HR and three clusters were identified via hierarchical clustering excluding eight proteins. Cluster 1 (65 proteins) showed a sequential decrease in expression from normal tissues to tumor tissues of LR and then to HR and was predominantly enriched in pathways such as tyrosine metabolism and ECM-receptor interaction, and increased matched mRNA expression of 18 proteins from this cluster predicted favorable prognosis. Cluster 2 (70 proteins) demonstrated a sequential increase in expression from normal tissues to tumor tissues of LR and then to HR and was mainly enriched in pathways such as extracellular organization, DNA replication and cell cycle, and high matched mRNA expression of 25 proteins indicated poor prognosis. Cluster 3 (49 proteins) showed high expression only in LR, with high matched mRNA expression of 20 proteins in this cluster indicating favorable prognosis. Furthermore, high expression of ERO1A and FEN1 at protein level predicted poor prognosis in early-stage LUAD, supporting the mRNA results. In conclusion, we discovered key differentially expressed proteins and pathways between low-risk and high-risk subtypes of early-stage LUAD. Some of these proteins could serve as potential biomarkers in prognostic evaluation.

Nuclear Disarmament and the Protection of Cultural Heritage Research paper sysadmin 6 October 2017

States possessing nuclear weapons should be called upon to consider and publish the risks posed to cultural heritage, and their mitigation strategies, in their nuclear-weapons doctrines and policies.

Summary

• Renewed risk assessments for nuclear weapons and policies are taking place around the world in light of nuclear modernization and the changing geostrategic environment that is making the use of nuclear weapons more likely. As such the humanitarian impacts of nuclear weapons and tests have received increased attention. However, the effect on cultural heritage has so far been neglected.
• The potential for armed conflict to destroy cultural heritage has been recognized in international law since 1954. There is significant evidence on the impact of nuclear weapons on cultural heritage including the consequences of their use in Hiroshima and Nagasaki and the effect of nuclear-testing programmes in places of cultural significance since 1945. States that possess nuclear weapons have increased liabilities and responsibilities to protect cultural heritage and cultural rights. The need to protect cultural heritage should strengthen the case for reducing and eliminating nuclear weapons.
• Failure to take into account the protection of heritage in the development of nuclear weapons policies – including disarmament, non-proliferation and arms-control negotiations – significantly undermines states’ existing commitments to protecting heritage threatened by conflict.
• Risk assessments of the impact of nuclear weapons on cultural heritage and important cultural artefacts – and methods of preventing such catastrophic damage – should be part of protecting cultural heritage in every country and the subject of informed public debate. A new body of knowledge on the full range of nuclear weapons impacts would introduce a fresh perspective to inform decision-makers, international organizations and the public in thinking about nuclear weapons policies and practices.
• Risk and resilience frameworks, which provide sets of solutions for risk assessments, would allow assessments of nuclear weapons threats to heritage and highlight vulnerabilities that need to be addressed. Such frameworks would provide a basis for policymakers to identify the world’s cultural heritage most at risk and help develop mitigation strategies to ensure that it is protected. In particular, states possessing nuclear weapons should be called upon to consider and publish the risks posed to cultural heritage, and their mitigation strategies, in their nuclear weapons doctrines and policies, as a contribution to transparency and confidence-building, and as a responsibility to the world’s shared heritage. International organizations, such as the UN Educational, Scientific, and Cultural Organization (UNESCO), have a role to play in bridging security perspectives with protecting cultural heritage.