How Lucknow's City Montessori Schools managed to offer comprehensive online education to its 57,000 students during the Covid-19 crisis

Minister, ADC Chairman, CSOs mourn RK Peter's demise

A method is presented for the automatic building of nucleotide chains into electron density which is fast enough to be used in interactive model-building software. Likely nucleotides lying in the vicinity of the current view are located and then grown into connected chains in a fraction of a second. When this development is combined with existing tools, assisted manual model building is as simple as or simpler than for proteins.

Chromatin is the complex assembly of nucleic acids and proteins that makes up the physiological form of the eukaryotic genome. The nucleosome is the fundamental repeating unit of chromatin, and is composed of ∼147 bp of DNA wrapped around a histone octamer formed by two copies of each core histone: H2A, H2B, H3 and H4. Prior to nucleosome assembly, and during histone eviction, histones are typically assembled into soluble H2A/H2B dimers and H3/H4 dimers and tetramers. A multitude of factors interact with soluble histone dimers and tetramers, including chaperones, importins, histone-modifying enzymes and chromatin-remodeling enzymes. It is still unclear how many of these proteins recognize soluble histones; therefore, there is a need for new structural tools to study non-nucleosomal histones. Here, a single-chain, tailless Xenopus H2A/H2B dimer was created by directly fusing the C-terminus of H2B to the N-terminus of H2A. It is shown that this construct (termed scH2BH2A) is readily expressed in bacteria and can be purified under non-denaturing conditions. A 1.31 Å resolution crystal structure of scH2BH2A shows that it adopts a conformation that is nearly identical to that of nucleosomal H2A/H2B. This new tool is likely to facilitate future structural studies of many H2A/H2B-interacting proteins.

Fragment-based molecular-replacement methods can solve a macromolecular structure quasi-ab initio. ARCIMBOLDO, using a common secondary-structure or tertiary-structure template or a library of folds, locates these with Phaser and reveals the rest of the structure by density modification and autotracing in SHELXE. The latter stage is challenging when dealing with diffraction data at lower resolution, low solvent content, high β-sheet composition or situations in which the initial fragments represent a low fraction of the total scattering or where their accuracy is low. SEQUENCE SLIDER aims to overcome these complications by extending the initial polyalanine fragment with side chains in a multisolution framework. Its use is illustrated on test cases and previously unknown structures. The selection and order of fragments to be extended follows the decrease in log-likelihood gain (LLG) calculated with Phaser upon the omission of each single fragment. When the starting substructure is derived from a remote homolog, sequence assignment to fragments is restricted by the original alignment. Otherwise, the secondary-structure prediction is matched to that found in fragments and traces. Sequence hypotheses are trialled in a brute-force approach through side-chain building and refinement. Scoring the refined models through their LLG in Phaser may allow discrimination of the correct sequence or filter the best partial structures for further density modification and autotracing. The default limits for the number of models to pursue are hardware dependent. In its most economic implementation, suitable for a single laptop, the main-chain trace is extended as polyserine rather than trialling models with different sequence assignments, which requires a grid or multicore machine. SEQUENCE SLIDER has been instrumental in solving two novel structures: that of MltC from 2.7 Å resolution data and that of a pneumococcal lipoprotein with 638 residues and 35% solvent content.

An online knowledge base on the alternate conformations adopted by main-chain and side-chain atoms in protein structures solved by X-ray crystallography is described.

A single-chain, tailless Xenopus laevis H2A/H2B dimer construct (scH2BH2A) was engineered by directly fusing the C-terminus of H2B to the N-terminus of H2A without an artificial linker sequence. A high-resolution crystal structure of scH2BH2A shows that it adopts a nearly identical fold to that of nucleosomal H2A/H2B and may be useful for future structural studies of many H2A/H2B-interacting proteins.

This study presents the crystal structure of a thiol variant of the human mitochondrial branched-chain aminotransferase protein. Human branched-chain aminotransferase (hBCAT) catalyzes the transamination of the branched-chain amino acids leucine, valine and isoleucine and α-ketoglutarate to their respective α-keto acids and glutamate. hBCAT activity is regulated by a CXXC center located approximately 10 Å from the active site. This redox-active center facilitates recycling between the reduced and oxidized states, representing hBCAT in its active and inactive forms, respectively. Site-directed mutagenesis of the redox sensor (Cys315) results in a significant loss of activity, with no loss of activity reported on the mutation of the resolving cysteine (Cys318), which allows the reversible formation of a disulfide bond between Cys315 and Cys318. The crystal structure of the oxidized form of the C318A variant was used to better understand the contributions of the individual cysteines and their oxidation states. The structure reveals the modified CXXC center in a conformation similar to that in the oxidized wild type, supporting the notion that its regulatory mechanism depends on switching the Cys315 side chain between active and inactive conformations. Moreover, the structure reveals conformational differences in the N-terminal and inter-domain region that may correlate with the inactivated state of the CXXC center.

Javad Fahanik-babaei, Bahareh Rezaee, Maryam Nazari, Nihad Torabi, Reza Saghiri, Remy Sauve, and Afsaneh Eliassi

We have determined the electropharmacological properties of a new potassium channel from brain mitochondrial membrane by planar lipid bilayer method. Our results showed the presence of a channel with a conductance of 150 pS at potentials between 0 and –60 mV in 200 cis/50 trans mM KCl solutions.

The channel was voltage-independent, with an open probability value ~0.6 at different voltages. ATP did not affect current amplitude and Po at positive and negative voltages. Notably, adding iberiotoxin, charybdotoxin, lidocaine, and margatoxin had no effect on the channel behavior. Similarly, no changes were observed by decreasing the cis-pH to 6. Interestingly, the channel was inhibited by adding sodium in a dose dependent manner. Our results also indicated a significant increase in mitochondrial complex IV activity and membrane potential and decrease in complex I activity and mitochondrial ROS production in the presence of sodium ions.

We propose that inhibition of mitochondrial K⁺ transport by Na ions on K⁺ channel opening may be important for cell protection and ATP synthesis.

The Emeco Alfi Chair is on view as part of ‘Making | Breaking New Arrivals,’ at Cooper Hewitt, Smithsonian Design Museum May–October, 2017. To learn […]

The post Cooper Hewitt: Emeco Alfi Chair Production Process slideshow appeared first on Smithsonian Insider.

The National Academy of Engineering has re-elected Gordon R. England, chairman of PFP Cybersecurity, to serve a two-year term as the NAEs chair.

Victoria A. Arroyo, executive director of the Georgetown Climate Center, and Leslie S. Richards, secretary of transportation for the Pennsylvania Department of Transportation (PennDOT), have been appointed as chair and vice chair, respectively, to the 2019 Transportation Research Board (TRB) Executive Committee, which provides oversight of TRB activities.

A new report from the National Academies of Sciences, Engineering, and Medicine recommends ways to make supply chains -- the systems that provide populations with critical goods and services, such as food and water, gasoline, and pharmaceuticals and medical supplies – more resilient in the face of hurricanes and other disasters, drawing upon lessons learned from the 2017 hurricanes Harvey, Irma, and Maria.

The National Academies of Sciences, Engineering, and Medicine announced today that Harvey Fineberg, former president of the Institute of Medicine (now the National Academy of Medicine) and current president of the Gordon and Betty Moore Foundation, will serve as the chair of the Standing Committee on Emerging Infectious Diseases and 21st Century Health Threats.

Larry Ellison, CEO of Oracle Corporation, gestures as he makes a speech during the New Economy Summit 2014 in this file photo taken in Tokyo on April 9, 2014. The company said Thursday, September 18, 2014, that Ellison would step aside as CEO and become chairman and chief technology officer.; Credit: TORU YAMANAKA/AFP/Getty Images

Oracle says Larry Ellison is stepping aside as CEO of the company he founded. The business software maker promoted Safra Catz and Mark Hurd to replace him as co-CEOs.

Ellison will reclaim the title of chairman at Oracle and is also taking the role of chief technology officer. Oracle says Ellison wants to focus on product engineering, technology development and strategy. Jeff Henley, Oracle's chairman since January 2004, is now its vice chairman.

Catz and Hurd were co-presidents of the Redwood Shores, California, company. Catz will be in charge of the company's manufacturing, finance and legal functions. Hurd will be in charge of sales, service, and other global business units.

Ellison founded Oracle Corp. in 1977 and was its chairman from May 1995 to January 2004.

Tradeshift, a supply...

If we can’t secure the supply chain, eventually everything else will break

The post What happens when the global supply chain breaks? appeared first on WeLiveSecurity

Researchers have discovered high levels of plastic particles and fibres, as well as black carbon (BC), which is formed by the incomplete burning of fossil fuels, in the waters of the Jade Bay, an inshore basin off the coast of Germany in the Southern North Sea. The concentration of suspended particles are of concern because they have the potential to be ingested by fish and other marine life, and enter the food chain.

Green supply chain management (GSCM) is a holistic approach to improving environmental performance along the supply chain. A new study by Italian researchers on over 4000 manufacturing facilities in seven developed countries has indicated that GSCM increases environmental performance.

The application to crops of struvite (magnesium ammonium phosphate) recovered from waste water may cause antibiotic resistance genes (ARGs) present in this fertiliser to enter the food chain. Chinese researchers who conducted this study on Brassica plants suggest that ARGs in struvite pass from the soil into the roots of the plant, and from the roots to the leaves, via the bacterial community already present. The results of this research highlight the need for struvite production methods and agricultural practices that minimise the risk of antibiotic-resistance transmission from struvite to humans or animals via the environment.

An analysis of European retailers revealed a range of initiatives being undertaken to improve the environmental performance of product supply chains. However, it appears that very large grocery retailers are less proactive than specialist and cooperative retailers, owing to their complex supply chains and tendency to consider consumers responsible for environmental improvements.

The pressures facing Indian manufacturers to ‘green’ their supply chain have been explored by a recent study. Both international and national pressures from government and consumers were found to play an important role in whether a company adopts green supply chain management principles, with pressures varying to some degree depending on the business’s sector and size.

A recent study from Finland on the carbon footprint of food can help producers and consumers make choices that lower the food chain’s impact on climate change, by highlighting hotspots in the food chain for improvement and revealing food’s overall impact.

Tracing products through the supply chain is a key feature for all businesses and industries; this is currently possible via automated identification systems that link a product to a database to track its progress, and through information-tracing (‘infotracing’) systems that keep accessible records of this progress. A study now introduces blockchain technology as a way to electronically trace timber as it travels from the forest to final product, using an infotracing system based on open source and Radio Frequency Identification (RFID) technology that retains records in a secure, decentralised, distributed ledger.

Mandatory legislation can play an important role in managers’ decisions to keep their supply chains ‘green’, according to a recent survey of Greek businesses which investigated incentives driving investment in green management practices.

Strict lockdown measures around the world has caused severe delays and logistical issues leading to worldwide PC shipments falling by 8%, technology market analyst firm Canalys said.

Biodiversity’s contribution to ecosystem services in grasslands — at different levels of the food chain (known as trophic levels1) — has been assessed in a new study. Higher species diversity across trophic levels — particularly for plants, insects and soil microbial decomposers — is important for the provision of multiple ecosystem services related to food production, recreational benefits, or climate regulation. Species diversity across different trophic groups was also found to be just as important in controlling ecosystem functioning as the management intensity of grasslands and environmental factors, such as climate or soil type.

Long-chain perfluoroalkyl acids (PFAAs) are persistent chemicals with proven toxic effects. This study estimated the emissions and concentrations of two such chemicals, perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), in 11 of Europe's most populated river catchments. Estimated emissions were lowest in the Thames and highest in the Rhine, while the EU environmental quality standard for PFOS was exceeded in all rivers. This study provides a picture of PFAAs contamination in rivers across Europe, and makes recommendations for achieving reductions.

Watford chairman and chief executive Scott Duxbury has labelled the Premier League's Project Restart plans as "unfair" and claims they are "lacking sporting integrity".

The “hip, cool and tech-oriented” chain will target millennials.

Sitting is bad for your health, so why not swap your desk seat with one of these solutions?

As commerce becomes increasingly global, American corporations are taking steps to develop sustainable supply chains that span the country and the planet.

The Danish capital's urban 'parkipelago' will eventually include a sauna, cafe and mussel farm.

No U.S. president has waxed as poetically about mining the moon the way John F. Kennedy did about landing on it.

Homemade chai tea graham crackers turn everyone’s favorite campfire dessert into an amazing ending to a dinner party.

Eco-friendly supply chain. According to a study by management consulting firm Accenture, companies have found that having an eco-friendly supply chain can save

The disintegration of a giant Antarctic ice shelf that had been stable for millennia was caused by a chain reaction of lakes draining on top of the ice.

In 'This Chair Rocks,' author Ashton Applewhite explains why everyone should be up arms about ageism.

Rudi Putra takes on powerful palm oil plantations in the name of conservation — and wins.

Health systems in emerging markets have not realized the benefits of data visibility to global health supply-chain operations. To do so, health-system leaders will need to take four critical actions.

Wired's video series, 'Five Levels of Difficulty,' challenges an expert to explain a complicated concept to people at five levels of expertise — and it's cool.

The constantly connected computer in your pocket makes it irrelevant.

Miss Willie the dog spent 12 years on a chain before she finally got to see the world.

Randell to take up role on 1 April.

Exclusive: The former Bluefin and Gallagher boss says he is taking on a number of new roles, with the rest to be revealed in the near future.