Juan Martin D'Ambrosio, Veronique Albanese, Nicolas-Frederic Lipp, Lucile Fleuriot, Delphine Debayle, Guillaume Drin, and Alenka Copic

Osh6 and Osh7 are lipid transfer proteins (LTPs) that move phosphatidylserine (PS) from the endoplasmic reticulum (ER) to the plasma membrane (PM). High PS level at the PM is key for many cellular functions. Intriguingly, Osh6/7 localize to ER-PM contact sites, although they lack membrane-targeting motifs, in contrast to multidomain LTPs that both bridge membranes and convey lipids. We show that Osh6 localization to contact sites depends on its interaction with the cytosolic tail of the ER-PM tether Ist2, a homologue of TMEM16 proteins. We identify a motif in the Ist2 tail, conserved in yeasts, as the Osh6-binding region, and we map an Ist2-binding surface on Osh6. Mutations in the Ist2 tail phenocopy osh6 osh7 deletion: they decrease cellular PS levels, and block PS transport to the PM. Our study unveils an unexpected partnership between a TMEM16-like protein and a soluble LTP, which together mediate lipid transport at contact sites.

Join Dr. Elizabeth Cottrell as she explores the volcanoes of Alaska’s Western Aleutian Islands.

The post Smithsonian volcanologist Elizabeth Cottrell explores Alaskan Volcanoes appeared first on Smithsonian Insider.

To catch lizards on the offshore islands close to St. Croix in the Caribbean, Smithsonian herpetologist Nicole Angeli uses a lasso of thread looped at […]

The post Mongooses wiped them out. Now Nicole Angeli wants the St. Croix ground lizard home again appeared first on Smithsonian Insider.

This article aims to encourage practitioners, young and seasoned, by enhancing their structure-determination toolboxes with a selection of tips and tricks on recognizing and handling aspects of data collection, structure modelling and refinement, and the interpretation of results.

In small-molecule single-crystal structure determination, we now have at our disposal an inspiring range of fantastic diffractometers with better, brighter sources, and faster, more sensitive detectors. Faster and more powerful computers provide integrated tools and software with impressive graphical user interfaces. Yet these tools can lead to the temptation not to check the work thoroughly and one can too easily overlook tell-tale signs that something might be amiss in a structure determination; validation with checkCIF is not always revealing. This article aims to encourage practitioners, young and seasoned, by enhancing their structure-determination toolboxes with a selection tips and tricks on recognizing and handling aspects that one should constantly be aware of. Topics include a pitfall when setting up data collections, the usefulness of reciprocal lattice layer images, processing twinned data, tips for disorder modelling and the use of restraints, ensuring hydrogen atoms are added to a model correctly, validation beyond checkCIF, and the derivation and interpretation of the final results.

The positional change of nitro­gen-7 of the RNA constituent guanosine to the bridgehead position-5 leads to the base-modified nucleoside 5-aza-7-de­aza­guanosine. Contrary to guanosine, this mol­ecule cannot form Hoogsteen base pairs and the Watson–Crick proton donor site N3—H becomes a proton-acceptor site. This causes changes in nucleobase recognition in nucleic acids and has been used to construct stable `all-purine' DNA and DNA with silver-mediated base pairs. The present work reports the single-crystal X-ray structure of 7-iodo-5-aza-7-de­aza­guanosine, C10H12IN5O5 (1). The iodinated nucleoside shows an anti conformation at the glycosylic bond and an N conformation (O4'-endo) for the ribose moiety, with an anti­periplanar orientation of the 5'-hy­droxy group. Crystal packing is controlled by inter­actions between nucleobase and sugar moieties. The 7-iodo substituent forms a contact to oxygen-2' of the ribose moiety. Self-pairing of the nucleobases does not take place. A Hirshfeld surface analysis of 1 highlights the contacts of the nucleobase and sugar moiety (O—H⋯O and N—H⋯O). The concept of pK-value differences to evaluate base-pair stability was applied to purine–purine base pairing and stable base pairs were predicted for the construction of `all-purine' RNA. Furthermore, the 7-iodo substituent of 1 was functionalized with benzo­furan to detect motional constraints by fluorescence spectroscopy.

During evaporation of natural and synthetic K–Mg–Cl brines, the formation of almost square plate-like crystals of potassium carnallite (potassium chloride magnesium dichloride hexa­hydrate) was observed. A single-crystal structure analysis revealed a monoclinic cell [a = 9.251 (2), b = 9.516 (2), c = 13.217 (4) Å, β = 90.06 (2)° and space group C2/c]. The structure is isomorphous with other carnallite-type com­pounds, such as NH4Cl·MgCl2·6H2O. Until now, natural and synthetic carnallite, KCl·MgCl2·6H2O, was only known in its ortho­rhom­bic form [a = 16.0780 (3), b = 22.3850 (5), c = 9.5422 (2) Å and space group Pnna].

A method is adapted to generate a full rank realization of an abstract regular polyhedron with automorphism group H3.

A new report from the National Academies of Sciences, Engineering, and Medicine outlines a research agenda for improving the commercial viability of technologies that turn greenhouse gases from the burning of fossil fuels into useful products such as fuels, construction materials, and chemicals.

Jiro's Pick this week is joyPlot by Santiago Benito.I must admit that I was simply drawn by the visualization, rather than the name of the function, as I was not familiar with the band or the music... read more >>

Director Eliza Hittman on the set of her film "Never Rarely Sometimes Always".
; Credit: Focus Features/Never Rarely Sometimes Always (2020)

The film “Never Rarely Sometimes Always” was slated for a theatrical release in March, but due to COVID-19 screenings were postponed. Instead, the film is out on digital this week, currently sporting a 98 percent rating on Rotten Tomatoes and receiving critical acclaim both here on FilmWeek and nationwide as one of the best films of 2020 so far.

Writer-director Eliza Hittman’s third feature-length film is about two teenage girls Skylar (Talia Ryder) and Autumn (Sidney Flanagan) from rural Pennsylvania who travel to New York City for medical help after an unplanned pregnancy. Hittman says the idea for the film came to her when she read in a book about how some women in Ireland, which up until recently had very strict laws against abortions, would travel from Ireland to London in 24 hours just to get a procedure. It struck her as worthy of a screenplay, and the idea was born. As part of her research for the film Hittman went to a small coal-mining community in rural Pennsylvania and, even though she wasn’t pregnant, visited pregnancy centers, got tested, and talked with women getting treatment and counseling so she could, as she says, “write the scenes with credibility.”

Today on FilmWeek, we’ll air “The Frame” host John Horn’s interview with “Never Rarely Sometimes Always” director Eliza Hittman where the two discuss how Hittman came up with the idea for the film, her journey to rural America to find out what visiting pregnancy centers there is like, and how that informed the way she conceived and wrote the film.

Guest:

Eliza Hittman, writer and director of “Never Rarely Sometimes Always”

This content is from Southern California Public Radio. View the original story at SCPR.org.

Hispanic Mexican city of Teotihuacan may have bred rabbits and hares for food, fur and bone tools, according to a study published August 17, 2016 in the open-access journal PLOS ONE by Andrew Somerville from the University of California San Diego, US, and colleagues.

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As more businesses yield the benefits of NLP-powered analytics and conversational interfaces, demand for single-vendor solutions will increase.

Researchers name the protein receptor 'Juno' after the Roman goddess of fertility.

Researcher Lindsey Swierk discovered that when water anoles flee underwater to escape predators, they stay underwater for a long time.

Legal cannabis markets are bringing a powerful ally to the fight against marijuana prohibition: The business community.

The brown anole is an invasive species, and if you can't beat 'em, eat 'em.

Pot growers have traditionally had a negative impact on the environment — but that may be about to change.

Mitsubishi Hitachi Power Systems (MHPS), Mitsubishi Heavy Industries Engineering (MHIENG) and Mitsubishi Gas Chemical Company (MGC) were selected by the New Energy and Industrial Technology Development Organization (NEDO) to conduct joint research on the effective recycling of carbon dioxide (CO2) emitted from the refinery at Tomakomai City, Hokkaido Japan where the CO2 is captured and stored by the existing demonstration plant. Further utilizing of the demonstration plant currently employed for CO2 Capture and Storage (CCS), the three companies will collaborate on research activities for CO2 Capture and Utilization (CCU) in order to produce methanol from captured CO2. The research is expected to run until February 2021.

Commission states it accepts the National Crime Agency’s conclusions that it found no evidence that any criminal offences had been committed after it investigated loans totalling £8m provided to the Leave.EU campaign from one of Banks' other companies.

Modern science and medicine has made it possible for many women to conceive that otherwise would be unable to bear children. If you are considering anything other than a natural pregnancy, explore the pros and cons of in-vitro fertilization first.

I would like to share my professional opinion on the benefits of dog training. Through training you will build a strong lifelong bond between you and your dog.

Atkinson is a Board Certified Otolaryngologist

Ms. Scharett has been endorsed by Marquis Who's Who as a leader in the fields of nursing and rehabilitation administration

Elizabeth A. Lamping has been recognized for excellence in professional services.

On January 25 and 26, 2019, The King's College will host the 2nd Annual American History and Western Civilization Challenge Bowl™, sponsored by the American Heritage Education Foundation.

On January 25 and 26, 2019, The King's College will host the 2nd Annual American History and Western Civilization Challenge Bowl™, sponsored by the American Heritage Education Foundation.

Dr. McGoldrick has been endorsed by Marquis Who's Who as a leader in the field of anesthesiology

Dr. King has been endorsed by Marquis Who's Who as a leader in the fields of communications, politics and higher education

Beta testing to be expanded to new installations; Management team now has over 100 years of combined industry experience with two new director-level appointments

This free Trip Planner is a perfect tool for travelers to discover new places, create a personalized itinerary, and have an organized vacation.

Ms. Coleman has been endorsed by Marquis Who's Who as a leader in the museum industry

Squishy Brains Studio announces the launch of a Kickstarter campaign for the "World of Humans" project.

StoneShot's Financial Marketer Mindset study reveals biggest challenges facing financial marketers today and the impact of technology on marketing strategy

H.H. Dorje Chang Buddha III was Compelled and Could Not Decline

Increasing accuracy in your models is often obtained through the first steps of data transformations. This guide explains the difference between the key feature scaling methods of standardization and normalization, and demonstrates when and how to apply each approach.

Interactive visualizations are an effective method for understanding the COVID-19 pandemic. This article presents a repository filled with just such insightful interactions.

At present, the data scientist is one of the most sought after professions. That’s one of the main reasons why we decided to cover the latest data visualization tools that every data scientist can use to make their work more effective.

Aprimo CMO says marketers are currently struggling with what he calls “digital sameness” — where everyone is doing the same thing online.

Please visit Marketing Land for the full article.

Pankaj Ghemawat, professor at NYU Stern and IESE business schools, debunks common misconceptions about the current state and extent of globalization. (Hint: the world is not nearly as globalized as people think.) He also discusses how popular reactions in Europe and the U.S. against globalization recently could affect the global economy, and how companies will need to adapt to the new reality. Ghemawat is the author of several books on globalization, including “World 3.0” and most recently “The Laws of Globalization and Business Applications.”

Andrew McAfee, co-director of the MIT Initiative on the Digital Economy, explains how the U.S. economy is growing and actually using less and less stuff to do so. Thanks to new technologies, many advanced economies are reducing their use of timber, metals, fertilizer, and other resources. McAfee says this dematerialization trend is spreading to other parts of the globe. While it’s not happening fast enough to stop climate change, he believes it offers some hope for environmental protection when combined with effective public policy. McAfee is the author of the book “More from Less: The Surprising Story of How We Learned to Prosper Using Fewer Resources—and What Happens Next.”

Throughout Joseph Weizenbaum's life, he liked to tell this story about a computer program he’d created back in the 1960s as a professor at MIT. It was a simple chatbot named ELIZA that could interact with users in a typed conversation. As he enlisted people to try it out, Weizenbaum saw similar reactions again and again -- people were entranced by the program. They would reveal very intimate details about their lives. It was as if they’d just been waiting for someone (or something) to ask. ELIZA was one of the first computer programs that could convincingly simulate human conversation, which Weizenbaum found frankly a bit disturbing.

The ELIZA Effect

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The Confederation of Real Estate Developers' Association of India (Credai) has written to minister for housing & urban affairs Hardeep Singh Puri, seeking urgent measures to control the hike in the prices of cement and steel amid the ongoing pandemic crisis.

According to research conducted around four coal-powered plants in Louisville, Kentucky, retiring coal has a noticeable effect on people's health.

The post As Coal Usage Declines, New Study Finds Dramatic Decrease in Asthma Symptoms and Hospitalizations appeared first on Good News Network.

A study of commercial timber harvesting activities in Oregon and Washington was conducted from 2011 through 2015 to characterize current tree utilization, logging operations, and assist with estimating the amount of woody biomass left onsite after harvesting.

A 4-year-old Douglas-fir plantation in the western Washington Cascades was monitored for 8 years after fertilization with potassium (K), nitrogen (N), and K+N to determine fertilizer effects on rates of mortality from laminated root rot (LRR) and other causes relative to a nonfertilized control.

We examine the use of woody residues, primarily from forest harvesting or wood products manufacturing operations (and to a limited degree from urban wood wastes), as a feedstock for direct-combustion bioenergy systems for electrical or thermal power applications. We examine opportunities for utilizing biomass for energy at several different scales, with an emphasis on larger scale electrical power generation at stand-alone facilities, and on smaller scale facilities (thermal heating only) such as governmental, educational, or other institutional facilities. We then identify west-wide barriers that tend to inhibit bioenergy applications, including accessibility, terrain, harvesting costs, and capital costs. Finally, we evaluate the role of government as a catalyst in stimulating new technologies and new uses of biomass material.