The Indian government's decision of starting the sale of liquor during lockdown to handle the states' economies, became a major topic of debate amongst people on social media. Some of them welcomed the decision and others criticised it by stating that

Two years ago, Galway lost their All-Ireland hurling crown to Limerick after a draining two-game semi-final against Clare.

Demonstrators describe an arrest and pepper ball use by IMPD during a protest over the death of Dreasjon Reed near 62nd Street and Michigan Road.

As Rafael, the leader of OM Romania, lies awake in the middle of the night, he shares about the OM outreach to the Roma people he and his family are taking part in. They’ve set up a mobile clinic in Padureni, and through games, sports and Bible stories, the community is learning about God’s love.

Nepal has raised objections over India inaugurating a strategic link road connecting Lipulekh pass at a height of 17,000 feet along the border with China in Uttarakhand with Dharchula. Nepal's...

The Hero Duet could be replaced by something that was showcased at the Auto Expo 2016 but could still be undergoing development.

The Ducati Panigale V2 draws power from a 955cc, V-twin Superquadro engine which is now BS6/Euro5 compliant and pumps out 151 horses.

If this isn't real yet, surely it soon will be...

The teams are being sent to Gujarat, Tamil Nadu, Uttar Pradesh, Delhi, Rajasthan, Madhya Pradesh, Punjab, West Bengal, Andhra Pradesh and Telangana.

The Brazilian government is seeking to award contracts in an auction tomorrow for natural gas- and coal-fueled power plants, reversing a drive that previously favored renewable-energy projects. It would lead to the first new thermal plants in three years, after the government scaled back such projects and awarded wind contracts starting in 2009 and solar energy earlier this year.

More than a year after Prime Minister David Cameron publicly announced support for the Perpetuus Tidal Energy Center (PTEC), Great Britain’s Marine Management Organization (MMO) issued a license on April 20 to Royal HaskoningDHV to deploy and operate a proposed 30-MW tidal array at the center, located off the Isle of Wight.

There are no plans for new coal plants to be built in the United States. This opens doors for the geothermal industry possibly more than ever before in U.S. history. In an Energy Information Administration (EIA) forecast to 2018, coal was one of the top-cost commissioning technology options; geothermal was one of the lowest.

The Brazilian government is seeking to award contracts in an auction tomorrow for natural gas- and coal-fueled power plants, reversing a drive that previously favored renewable-energy projects. It would lead to the first new thermal plants in three years, after the government scaled back such projects and awarded wind contracts starting in 2009 and solar energy earlier this year.

Chelsea winger, Willian, is believed to be mulling over a three-year deal from Arsenal, Daily Cannon reports. Willian is set to become a free agent this summer, when his current deal runs out at Stamford Bridge. With the 2019/2020 campaign still to be concluded, players who are out of contract at the end of June […]

EPL: Willian offered three-year deal to leave Chelsea for Arsenal

The Department for Work and Pensions has confirmed that GP sick notes are to be replaced by 'fit notes' with effect from 6 April 2010. The Government consulted on the design and content of the new medical certificate last ...

Introduction The long-awaited regulations on the Administration of Commercial Franchise Operations (the 'New Regulations') were promulgated by the State Council of the PRC on 6 February 2007, and will take effect on 1 May 2007. They apply equally t...

Relive the excitement of Women's Soccer from the 2016 Rio Summer Olympics.

Boris Johnson is to replace his "stay at home" slogan with a fresh rallying cry to the nation, as the battle against coronavirus rages on.

Akash Kamble fell in love when he was a little boy. And once he set eyes on her languid, unending, lithe body, he was hooked.

His mother would take him along on the Mumbai local, from Bhayandar to Charni Road every day, so that her in-laws could babysit while she finished a day's work at her Fort office. Travelling in the train, watching it fly past through the window grill, had Kamble realise he loved trains deeply enough to work around them.

To acquire the weather beaten look of a diesel locomotive, Kamble uses candle light against the aluminium sheets

"I would observe them carefully during the commute and went on to make my first cardboard train model of a two-coach local when I was in Class VIII," says Kamble, 23, who is a mechanical engineer and holds the job of a Station Master with the Mumbai Monorail.

The rail model enthusiast thought the lockdown was the perfect opportunity to wrap up a few more models. He has, in the last five weeks, made 11 models of hi-class trains, from locomotives to local coaches.

"I first started with cardboard and foam-based train and engine models, but later moved to working with aluminium. My research involves reading about trains, their specifications and poring over their drawings. I make models of 1:35 scale, realistic and a perfect match [to the original]. The continuous involvement slowly leads to perfection," says Kamble, a resident of Ambernath, adding, "I have made a model of the most powerful green coloured WAG-9 electric locomotive and two coaches of the Garib Rath train. Besides this, one of my favourites is the diesel locomotive, a few coaches of the Mumbai local and a mail express."

He has also found the time to give finishing touches to earlier models, giving them a realistic feel. To acquire a weather beaten look of a diesel locomotive, he uses candle light against the aluminium sheets.

In 2019, Kamble joined what he calls his dream job. And, next on his agenda is to make a "replica of my employer". He has acquired drawings and technical specifications of the Mumbai Monorail and hopes to complete the project before the city chugs back to normal.

1:35
The ratio scale to which the mechanical engineer makes his models

Catch up on all the latest Mumbai news, crime news, current affairs, and a complete guide from food to things to do and events across Mumbai. Also download the new mid-day Android and iOS apps to get latest updates.

Mid-Day is now on Telegram. Click here to join our channel (@middayinfomedialtd) and stay updated with the latest news

"Jack Reacher: Never Go Back"; Director: Edward Zwick; Cast: Tom Cruise, Cobie Smulders, Aldis Hodge, Danika Yarosh, Patrick Heusinger, Holt McCallany, Robert Knepper; Rating: **1/2

In 2012, the Joint Meeting agreed on the status of deleted and former versions of Test Guidelines with regards to the Mutual Acceptance of Data (MAD). An 18-month transitional period between the Council Decision and the effective date of deletion was agreed, after which no new test using the deleted or the former version of a Test Guideline can be initiated.

1. OnePlus slipped to third position in India's premium smartphone segment in Q1 2020, iPhone 11 was the top selling device  gizmochina
2. Samsung releases One UI 2.0 update for Galaxy A20s  NewsBytes
3. Features we want and don’t want from the Galaxy Note 20  SamMobile
4. Leaked Samsung Galaxy A21s render shows a familiar design - GSMArena.com news  GSMArena.com
5. Samsung is Now the Top Brand for Premium Smartphones in India  Gizchina.com
6. View Full coverage on Google News

United manager Ole Gunnar Solskjaer revealed ahead of his side's game with Leicester City that the Spaniard's future remains up in the air as contract talks over a new £375,000-per-week deal.

Pia Miller's nearest and dearest certainly know how to spoil her.  

नयी दिल्ली। देश के कुछ हिस्सों में लॉकडाउन में दी गई ढील के बीच कई फोन कंपनियों ने अपनी सेवाएं फिर से शुरू कर दी हैं। शानदार स्मार्टफोन बनाने वाली वनप्लस ने एक बार फिर अपनी वनप्लस 7 सीरीज़ और वनप्लस

Kamal Haasan, the Ulaganayagan, recently had an chat with the talented actor Vijay Sethupathi through social media. Interestingly, the online interaction of the actors was titled as 'Thalaivan Irukkindran'. To the uninitiated, Thalaivan Irukkindran is the title of Kamal Haasan's upcoming project,

Archives, Room Use Only - TK5613.U6 C66 1864

Delhi Police claimed to be dissatisfied by the response it got from social media platform Instagram in the case of ‘Bois Locker Room’, where a group of male students made sexual threats to girls and carried on salacious conversations about their female schoolmates. On Saturday, Delhi Commission for Women also sent a second notice to police on the case after a girl student alleged receiving threats.

The police deployment has come after a fringe right-wing group, Hindu Sena, gave a call to clear the Shaheen Bagh road on March 1.

6 EPL teams oppose restart: Watford chief

Sex chatroom: Police not happy with Insta reply

Migrants, tribals have no money to buy food due to lockdown: PIL

Molecular replacement (MR) is the predominant route to solution of the phase problem in macromolecular crystallography. Where the lack of a suitable homologue precludes conventional MR, one option is to predict the target structure using bioinformatics. Such modelling, in the absence of homologous templates, is called ab initio or de novo modelling. Recently, the accuracy of such models has improved significantly as a result of the availability, in many cases, of residue-contact predictions derived from evolutionary covariance analysis. Covariance-assisted ab initio models representing structurally uncharacterized Pfam families are now available on a large scale in databases, potentially representing a valuable and easily accessible supplement to the PDB as a source of search models. Here, the unconventional MR pipeline AMPLE is employed to explore the value of structure predictions in the GREMLIN and PconsFam databases. It was tested whether these deposited predictions, processed in various ways, could solve the structures of PDB entries that were subsequently deposited. The results were encouraging: nine of 27 GREMLIN cases were solved, covering target lengths of 109–355 residues and a resolution range of 1.4–2.9 Å, and with target–model shared sequence identity as low as 20%. The cluster-and-truncate approach in AMPLE proved to be essential for most successes. For the overall lower quality structure predictions in the PconsFam database, remodelling with Rosetta within the AMPLE pipeline proved to be the best approach, generating ensemble search models from single-structure deposits. Finally, it is shown that the AMPLE-obtained search models deriving from GREMLIN deposits are of sufficiently high quality to be selected by the sequence-independent MR pipeline SIMBAD. Overall, the results help to point the way towards the optimal use of the expanding databases of ab initio structure predictions.

Although the success of molecular-replacement techniques requires the solution of a six-dimensional problem, this is often subdivided into two three-dimensional problems. REMO09 is one of the programs which have adopted this approach. It has been revisited in the light of a new probabilistic approach which is able to directly derive conditional distribution functions without passing through a previous calculation of the joint probability distributions. The conditional distributions take into account various types of prior information: in the rotation step the prior information may concern a non-oriented model molecule alone or together with one or more located model molecules. The formulae thus obtained are used to derive figures of merit for recognizing the correct orientation in the rotation step and the correct location in the translation step. The phases obtained by this new version of REMO09 are used as a starting point for a pipeline which in its first step extends and refines the molecular-replacement phases, and in its second step creates the final electron-density map which is automatically interpreted by CAB, an automatic model-building program for proteins and DNA/RNA structures.

Good prior estimates of the effective root-mean-square deviation (r.m.s.d.) between the atomic coordinates of the model and the target optimize the signal in molecular replacement, thereby increasing the success rate in difficult cases. Previous studies using protein structures solved by X-ray crystallography as models showed that optimal error estimates (refined after structure solution) were correlated with the sequence identity between the model and target, and with the number of residues in the model. Here, this work has been extended to find additional correlations between parameters of the model and the target and hence improved prior estimates of the coordinate error. Using a graph database, a curated set of 6030 molecular-replacement calculations using models that had been solved by X-ray crystallography was analysed to consider about 120 model and target parameters. Improved estimates were achieved by replacing the sequence identity with the Gonnet score for sequence similarity, as well as by considering the resolution of the target structure and the MolProbity score of the model. This approach was extended by analysing 12 610 additional molecular-replacement calculations where the model was determined by NMR. The median r.m.s.d. between pairs of models in an ensemble was found to be correlated with the estimated r.m.s.d. to the target. For models solved by NMR, the overall coordinate error estimates were larger than for structures determined by X-ray crystallography, and were more highly correlated with the number of residues.

Many biologists are now routinely seeking to determine the three-dimensional structures of their proteins of choice, illustrating the importance of this knowledge, but also of the simplification and streamlining of structure-determination processes. Despite the fact that most software packages offer simple pipelines, for the non-expert navigating the outputs and understanding the key aspects can be daunting. Here, the structure determination of the type IV pili (TFP) protein PilA1 from Clostridioides difficile is used to illustrate the different steps involved, the key decision criteria and important considerations when using the most common pipelines and software. Molecular-replacement pipelines within CCP4i2 are presented to illustrate the more commonly used processes. Previous knowledge of the biology and structure of TFP pilins, particularly the presence of a long, N-terminal α-helix required for pilus formation, allowed informed decisions to be made during the structure-determination strategy. The PilA1 structure was finally successfully determined using ARCIMBOLDO and the ab initio MR strategy used is described.

The performance of automated protein model building usually decreases with resolution, mainly owing to the lower information content of the experimental data. This calls for a more elaborate use of the available structural information about macromolecules. Here, a new method is presented that uses structural homologues to improve the quality of protein models automatically constructed using ARP/wARP. The method uses local structural similarity between deposited models and the model being built, and results in longer main-chain fragments that in turn can be more reliably docked to the protein sequence. The application of the homology-based model extension method to the example of a CFA synthase at 2.7 Å resolution resulted in a more complete model with almost all of the residues correctly built and docked to the sequence. The method was also evaluated on 1493 molecular-replacement solutions at a resolution of 4.0 Å and better that were submitted to the ARP/wARP web service for model building. A significant improvement in the completeness and sequence coverage of the built models has been observed.

Fragment-based molecular replacement exploits the use of very accurate yet incomplete search models. In the case of the ARCIMBOLDO programs, consistent phase sets produced from the placement and refinement of fragments with Phaser can be combined in order to increase their signal before proceeding to the step of density modification and autotracing with SHELXE. The program ALIXE compares multiple phase sets, evaluating mean phase differences to determine their common origin, and subsequently produces sets of combined phases that group consistent solutions. In this work, its use on different scenarios of very partial molecular-replacement solutions and its performance after the development of a much-optimized set of algorithms are described. The program is available both standalone and integrated within the ARCIMBOLDO programs. ALIXE has been analysed to identify its rate-limiting steps while exploring the best parameterization to improve its performance and make this software efficient enough to work on modest hardware. The algorithm has been parallelized and redesigned to meet the typical landscape of solutions. Analysis of pairwise correlation between the phase sets has also been explored to test whether this would provide additional insight. ALIXE can be used to exhaustively analyse all partial solutions produced or to complement those already selected for expansion, and also to reduce the number of redundant solutions, which is particularly relevant to the case of coiled coils, or to combine partial solutions from different programs. In each case parallelization and optimization to provide speedup makes its use amenable to typical hardware found in crystallography. ARCIMBOLDO_BORGES and ARCIMBOLDO_SHREDDER now call on ALIXE by default.

Model quality assessment programs estimate the quality of protein models and can be used to estimate local error in protein models. ProQ3D is the most recent and most accurate version of our software. Here, it is demonstrated that it is possible to use local error estimates to substantially increase the quality of the models for molecular replacement (MR). Adjusting the B factors using ProQ3D improved the log-likelihood gain (LLG) score by over 50% on average, resulting in significantly more successful models in MR compared with not using error estimates. On a data set of 431 homology models to address difficult MR targets, models with error estimates from ProQ3D received an LLG of >50 for almost half of the models 209/431 (48.5%), compared with 175/431 (40.6%) for the previous version, ProQ2, and only 74/431 (17.2%) for models with no error estimates, clearly demonstrating the added value of using error estimates to enable MR for more targets. ProQ3D is available from http://proq3.bioinfo.se/ both as a server and as a standalone download.

The Kepler spacecraft was launched in March of 2009 to study extrasolar planets. One of its major goals is the detection of terrestrial planets in habitable zones.

The post Kepler spacecraft used by Smithsonian astronomers to find other earths appeared first on Smithsonian Insider.

On the Smithsonian Tropical Research Institute's Barro Colorado Island in the Panama Canal, staff members are taking down a network of seven tall Automated Radio Telemetry System towers used to track animals wearing radio-transmitters. Scientists on the island are switching to GPS and camera trap systems to produce more data with less infrastructure.

The post GPS and camera traps to replace radio antennas in tracking animals on Barro Colorado Island appeared first on Smithsonian Insider.

The Harvard-Smithsonian Center for Astrophysics continues to be a major player in the planet-hunting realm. It is part of an international collaboration building a new instrument called HARPS-North.

The post HARPS-N instrument will help confirm Kepler’s planet finds appeared first on Smithsonian Insider.

Last week, the Kepler team and CfA astronomers announced the discovery of a system of six transiting planets around one Sun-like star. The previous record holder for the number of transiting planets was three.

The post Six orbiting planets sets record for Sun-like stars say Kepler, Smithsonian astronmers appeared first on Smithsonian Insider.

NASA's Kepler spacecraft is proving itself to be a prolific planet hunter. Within just the first four months of data, astronomers have found evidence for more than 1,200 planetary candidates. Of those, 408 reside in systems containing two or more planets, and most of those look very different than our solar system.

The post The Kepler spacecraft’s astounding haul of multiple-planet systems appeared first on Smithsonian Insider.

NASA's Kepler spacecraft has spotted a planet that alternately runs late and
early in its orbit because a second, "invisible" world is tugging on it.
This is the first definite detection of a previously unknown planet using
this method.

The post Invisible world “spotted” tugging on visible planet by Kepler spacecraft appeared first on Smithsonian Insider.