The sense of celebration is palpable throughout and if this indeed does turn out to be the end of the band, what an end it is.

Los representantes de CBZ Management han creado una campaña pública para culpar los problemas del complejos de apartamentos de Aurora a las actividades recientes de las pandillas.

Get ready to receive Tamia's heart and soul!

He is suspected to have died from an overdose.

The Philatelic Bureau of the Bermuda Post Office will be releasing a new commemorative stamp issue, “Backyard Birds” on Thursday, September 21, 2023. A Government spokesperson said, “Today, nine songbird species live in backyards across the Bermuda archipelago. Of these, seven were introduced by humans, while two species are indigenous to Bermuda, their North American […]

The Bermuda Post Office [BPO] announced the latest commemorative stamp issue ~ “Mary Prince”. A Government spokesperson said, “In keeping with this month’s Emancipation observations, the Bermuda Post Office [BPO] is pleased to advise of the latest commemorative stamp issue ~ ‘Mary Prince’. This newest stamp series features imagery and renditions of Bermuda’s National Hero Mary […]

Žije v něm podobný počet lidí jako v České republice, rozlohou je třikrát větší a kulturně mnohem rozmanitější. Michigan, stát nacházející se na severovýchodě USA, má možná to nejlepší za sebou, přesto patří k nejdůležitějším státům v zemi. Až do roku 2016 byl Michigan sice nejistým státem, ale zato jasně tíhnoucím k demokratům. Donald Trump ukázal, že to není pravda, a navýšil svůj náskok o 70 tisíc hlasů oproti volbám před osmi lety. Bývalému prezidentovi se povedlo voliče mobilizovat, Kamale Harris naopak demobilizovat. Pohoření demokratů v Michiganu ukazuje chyby, které je stály volitele napříč celou Amerikou.

Bantam Tools Acquires Evil Mad Scientist to Accelerate Development of Next Generation Art and Handwriting Machines Windell Oskay and Lenore Edman of Evil Mad Scientist named CTO and COO of Bantam Tools  PEEKSKILL, N.Y, January 16, 2024 — Bantam Tools, the desktop CNC manufacturer that builds exceptional computer controlled machines for innovators, is excited to … Continue reading Bantam Tools Acquires Evil Mad Scientist →

Bantam Tools proudly unveils its latest advancement in proven plotter technology with the launch of the new Bantam Tools NextDraw™ series of drawing and handwriting machines. Engineered for artists, innovators, and educators seeking exceptional versatility and performance, Bantam Tools now offers a trio of new models designed for drawing and handwriting. “The Evil Mad Scientist … Continue reading Introducing the Bantam Tools NextDraw™ →

Welcome to the heart and soul of Chelsea Football Club – Stamford Bridge. This revered stadium stands not only as..

The post Stamford Bridge: More than a Stadium appeared first on Chelsea FC Blog.

A few years ago, I had a successful KonMari sesh that helped me tackle my closet chaos. But over time, I noticed that clutter started to creep back in. The issue was: All In, No Out. I made new purchases—thanks to an expanding waistline—but neglected to let go of ill-fitting clothes (because maybe, just maybe, I may fit into them again?) But recently, after spending a good 10 minutes digging through my wardrobe for that particular mustard cardigan, it became […]

The post 10 Things From IKEA Helped Me Tame Closet Chaos (Finally!) appeared first on IKEA Hackers.

Soil erosion is an age-long predicament that always shows up whenever humans embark on any land use activity. Whether it is agriculture, construction or gardening, the need to tame erosion is apparent, especially in regions that experience significant precipitation. The U.N. Food and Agriculture Organization (FAO) warns that 90% of the world’s topsoil risks erosion by 2050. It is, therefore, the responsibility of those who participate in land use activities to prevent such a mess. [...]

Một số nhà quan sát nhận định việc Campuchia rút khỏi Tam giác Phát triển Campuchia-Lào-Việt Nam là quyết định đột ngột đối với cả Việt Nam lẫn Lào, nhất là khi chỉ một tháng trước, Việt Nam còn nhấn mạnh tầm quan trọng của dự án này.

Các quan chức an ninh Đức nói với Bộ trưởng Công an Lương Tam Quang rằng những vụ việc như vụ bắt cóc Trịnh Xuân Thanh "không được tái diễn".

Thanks for joining us today about what the New Testament has to say about how to please God. If you missed last week’s study concerning the Old Testament, please read it now. While we wait, here is a “pleasing” song. Last week I offered my take on pleasing God which is always at the top […]

The post A Quick Bible Study Vol. 240: What the Old Testament Says About How to Please God appeared first on Liberty Unyielding.

Roland Bruderer
May 1, 2015; 14:1400-1410
Research

Lys234 is one of the residues present in class A β-lactamases that is under selective pressure due to antibiotic use. Located adjacent to proton shuttle residue Ser130, it is suggested to play a role in proton transfer during catalysis of the antibiotics. The mechanism underpinning how substitutions in this position modulate inhibitor efficiency and substrate specificity leading to drug resistance is unclear. The K234R substitution identified in several inhibitor-resistant β-lactamase variants is associated with decreased potency of the inhibitor clavulanic acid, which is used in combination with amoxicillin to overcome β-lactamase–mediated antibiotic resistance. Here we show that for CTX-M-14 β-lactamase, whereas Lys234 is required for hydrolysis of cephalosporins such as cefotaxime, either lysine or arginine is sufficient for hydrolysis of ampicillin. Further, by determining the acylation and deacylation rates for cefotaxime hydrolysis, we show that both rates are fast, and neither is rate-limiting. The K234R substitution causes a 1500-fold decrease in the cefotaxime acylation rate but a 5-fold increase in kcat for ampicillin, suggesting that the K234R enzyme is a good penicillinase but a poor cephalosporinase due to slow acylation. Structural results suggest that the slow acylation by the K234R enzyme is due to a conformational change in Ser130, and this change also leads to decreased inhibition potency of clavulanic acid. Because other inhibitor resistance mutations also act through changes at Ser130 and such changes drastically reduce cephalosporin but not penicillin hydrolysis, we suggest that clavulanic acid paired with an oxyimino-cephalosporin rather than penicillin would impede the evolution of resistance.

The bacterial enhancer-binding protein (bEBP) FlrC, controls motility and colonization of Vibrio cholerae by regulating the transcription of class-III flagellar genes in σ54-dependent manner. However, the mechanism by which FlrC regulates transcription is not fully elucidated. Although, most bEBPs require nucleotides to stimulate the oligomerization necessary for function, our previous study showed that the central domain of FlrC (FlrCC) forms heptamer in a nucleotide-independent manner. Furthermore, heptameric FlrCC binds ATP in “cis-mediated” style without any contribution from sensor I motif 285REDXXYR291 of the trans protomer. This atypical ATP binding raises the question of whether heptamerization of FlrC is solely required for transcription regulation, or if it is also critical for ATPase activity. ATPase assays and size exclusion chromatography of the trans-variants FlrCC-Y290A and FlrCC-R291A showed destabilization of heptameric assembly with concomitant abrogation of ATPase activity. Crystal structures showed that in the cis-variant FlrCC-R349A drastic shift of Walker A encroached ATP-binding site, whereas the site remained occupied by ADP in FlrCC-Y290A. We postulated that FlrCC heptamerizes through concentration-dependent cooperativity for maximal ATPase activity and upon heptamerization, packing of trans-acting Tyr290 against cis-acting Arg349 compels Arg349 to maintain proper conformation of Walker A. Finally, a Trp quenching study revealed binding of cyclic-di-GMP with FlrCC. Excess cyclic-di-GMP repressed ATPase activity of FlrCC through destabilization of heptameric assembly, especially at low concentration of protein. Systematic phylogenetic analysis allowed us to propose similar regulatory mechanisms for FlrCs of several Vibrio species and a set of monotrichous Gram-negative bacteria.

Vitamin A aldehyde covalently bound to opsin protein is embedded in a phospholipid-rich membrane that supports photon absorption and phototransduction in photoreceptor cell outer segments. Following absorption of a photon, the 11-cis-retinal chromophore of visual pigment in photoreceptor cells isomerizes to all-trans-retinal. To maintain photosensitivity 11-cis-retinal must be replaced. At the same time, however, all-trans-retinal has to be handled so as to prevent nonspecific aldehyde activity. Some molecules of retinaldehyde upon release from opsin are efficiently reduced to retinol. Other molecules are released into the lipid phase of the disc membrane where they form a conjugate (N-retinylidene-PE, NRPE) through a Schiff base linkage with phosphatidylethanolamine (PE). The reversible formation of NRPE serves as a transient sink for retinaldehyde that is intended to return retinaldehyde to the visual cycle. However, if instead of hydrolyzing to PE and retinaldehyde, NRPE reacts with a second molecule of retinaldehyde a synthetic pathway is initiated that leads to the formation of multiple species of unwanted bisretinoid fluorophores. We report on recently identified members of the bisretinoid family some of which differ with respect to the acyl chains associated with the glycerol backbone. We discuss processing of the lipid moieties of these fluorophores in lysosomes of retinal pigment epithelial (RPE) cells, their fluorescence characters and new findings related to light and iron-associated oxidation of bisretinoids.

Atherosclerosis is characterized by the pathological accumulation of cholesterol-laden macrophages in the arterial wall. Atherosclerosis is also the main underlying cause of CVDs, and its development is largely driven by elevated plasma cholesterol. Strong epidemiological data find an inverse association between plasma β-carotene with atherosclerosis, and we recently showed that β-carotene oxygenase 1 (BCO1) activity, responsible for β-carotene cleavage to vitamin A, is associated with reduced plasma cholesterol in humans and mice. In this study, we explore whether intact β-carotene or vitamin A affects atherosclerosis progression in the atheroprone LDLR-deficient mice. Compared with control-fed Ldlr^–/– mice, β-carotene-supplemented mice showed reduced atherosclerotic lesion size at the level of the aortic root and reduced plasma cholesterol levels. These changes were absent in Ldlr^–/–/Bco1^–/– mice despite accumulating β-carotene in plasma and atherosclerotic lesions. We discarded the implication of myeloid BCO1 in the development of atherosclerosis by performing bone marrow transplant experiments. Lipid production assays found that retinoic acid, the active form of vitamin A, reduced the secretion of newly synthetized triglyceride and cholesteryl ester in cell culture and mice. Overall, our findings provide insights into the role of BCO1 activity and vitamin A in atherosclerosis progression through the regulation of hepatic lipid metabolism.

Vitamin D supplements might lower blood pressure in seniors who are obese, reducing their heart health risk, a new study says.

It will cost $55.7 million to repair Tropicana Field, home to the MLB Tampa Bay Rays, in time for the 2026 home opener, St. Petersburg., Fla., city officials said Tuesday.

Tianeptine, an addictive drug at high doses, is being sold as a dietary supplement in gas stations and convenient stores. But such products could be contaminated with metals, microorganisms or other undisclosed drugs

Levels are typically lower in the body through the winter months in northern latitudes.

The study found that 68% were vitamin D deficient.

Nora D. Volkow
Dec 1, 2001; 21:9414-9418
Behavioral

Danilo De Gregorio
Feb 3, 2021; 41:891-900
Symposium and Mini-Symposium

We used virus-mediated anterograde and retrograde tracing, optogenetic modulation, immunostaining, in situ hybridization, and patch-clamp recordings in acute brain slices to study the release mechanism and μ-opioid modulation of the dual glutamatergic/GABAergic inputs from the ventral tegmental area and supramammillary nucleus to the granule cells of the dorsal hippocampus of male and female mice. In keeping with previous reports showing that the two transmitters are released by separate active zones within the same terminals, we found that the short-term plasticity and pharmacological modulation of the glutamatergic and GABAergic currents are indistinguishable. We further found that glutamate and GABA release at these synapses are both virtually completely mediated by N- and P/Q-type calcium channels. We then investigated μ-opioid modulation of these synapses and found that activation of μ-opioid receptors (MORs) strongly inhibits the glutamate and GABA release, mostly through inhibition of presynaptic N-type channels. However, the modulation by MORs of these dual synapses is complex, as it likely includes also a disinhibition due to downmodulation of local GABAergic interneurons which make direct axo-axonic contacts with the dual glutamatergic/GABAergic terminals. We discuss how this opioid modulation may enhance LTP at the perforant path inputs, potentially contributing to reinforce memories of drug-associated contexts.

See how geometric designs are inspiring new ways to make stretchy materials. (Video by Ahmad Rafsanjani and Damiano Pasini/McGill University)

Read more about FDR at http://www.smithsonianmag.com/arts-culture/From-the-Castle-FDRs-Stamps.html A stamp collector since childhood, Franklin Roosevelt designed postage stamps to help promote his presidential agenda.

Selected by Eli Reed for our special issue, this up-and-coming photographer discusses his work

Massive distribution efforts by OM teams in Bangladesh over more than 10 years sowed gospel seeds and made the Bengali New Testament a #1 best seller.

Claims include public nuisance, trespass and unjust enrichment Delaware Attorney General Kathy Jennings filed a lawsuit Wednesday against agrochemical giant Monsanto and two of its corporate spinoffs for long-lasting damage to Delaware’s natural resources. The lawsuit seeks to recover damages and clean-up costs associated with polychlorinated biphenyls (PCBs), extremely toxic and persistent chemicals which Monsanto […]

Defendant faces multiple felonies after stealing drug evidence  The Department of Justice’s Division of Civil Rights & Public Trust has indicted a Millsboro police sergeant and evidence custodian after an investigation revealed that he tampered with and used drugs stored in the Department’s evidence locker.   Matthew Dufour, 34, faces ten charges—including multiple felonies such […]

Attorney General seeks damages, including costs necessary to restore impacted natural resources and funding for State-run public health programs. Attorney General Kathleen Jennings announced that the Department of Justice has filed a lawsuit to hold numerous companies accountable for contaminating Delaware’s natural resources with per- and poly-fluoroalkyl substances (PFAS) traceable to the use and disposal […]

Delaware wildlife artist Richard Clifton and Nebraskan artist Dennis Arp took top honors in DNREC's annual Waterfowl Stamp and Trout Stamp art contests, with Clifton's painting of a Canada goose for the 2025/26 Delaware Waterfowl Stamp and Arp's painting of a brook trout for the 2025 Delaware Trout Stamp.

The Thoothukudi police in Tamil Nadu have arrested a physical education teacher at a private school for allegedly harassing girl students sexually and forcing them to take liquor.

1. Ricky Ponting hits back at Gautam Gambhir, calls him 'prickly character'  The Times of India
2. Ricky Ponting Fires Back At Gautam Gambhir After India Coach's Press Conference Remarks  NDTV Sports
3. A fired-up Virat Kohli is Australia's worry after Ricky Ponting's 'bad move'  The Times of India
4. Gambhir a prickly character, never took dig at Kohli: Ponting  The Hindu
5. Border-Gavaskar Trophy: Why Gautam Gambhir comes across as abrasive at times  The Indian Express

1. Chennai rain: IMD predicts heavy rainfall for Tamil Nadu. Are schools, colleges closed today?  Hindustan Times
2. Chennai rains today: Heavy downpour expected in many Tamil Nadu districts, IMD issues alert. Schools shut today?  Mint
3. Low pressure weakens, but heavy rain in Tamil Nadu may persist  The New Indian Express
4. Tamil Nadu weather: Schools shut in Chennai, IMD puts 17 districts in state on heavy rainfall alert  Business Today
5. Tamil Nadu rains: Schools, colleges closed in several districts amid heavy rain  The Hindu

[The Edelstam Foundation] The Edelstam Prize 2024 is awarded to Mr. Dawit Isaak for his outstanding contributions and exceptional courage in standing up for freedom of expression, one's beliefs, and in the defence of Human Rights. The prize will be awarded during a ceremony at the House of Nobility in Stockholm, Sweden, on 19th November 2024. As Dawit Isaak is a prisoner of conscience and the longest-held detained journalist in the world, he cannot be present. His daughter, Betlehem Isaak, will receive the prize on his behalf.

bantam rotary hoe manual

Before Mesopotamian people invented writing, they used cylinder seals to press patterns into wet clay – and some of the symbols used were carried over into proto-writing

A Neanderthal skeleton was discovered encased in rock in an Italian cave 30 years ago, but it hasn’t been studied much due to a long-running impasse about how to safely excavate it