Björn Eismann, Teresa G. Krieger, Jürgen Beneke, Ruben Bulkescher, Lukas Adam, Holger Erfle, Carl Herrmann, Roland Eils, and Christian Conrad

3D cell cultures enable the in vitro study of dynamic biological processes such as the cell cycle, but their use in high-throughput screens remains impractical with conventional fluorescent microscopy. Here, we present a screening workflow for the automated evaluation of mitotic phenotypes in 3D cell cultures by light-sheet microscopy. After sample preparation by a liquid handling robot, cell spheroids are imaged for 24 hours in toto with a dual-view inverted selective plane illumination microscope (diSPIM) with a much improved signal-to-noise ratio, higher imaging speed, isotropic resolution and reduced light exposure compared to a spinning disc confocal microscope. A dedicated high-content image processing pipeline implements convolutional neural network based phenotype classification. We illustrate the potential of our approach by siRNA knock-down and epigenetic modification of 28 mitotic target genes for assessing their phenotypic role in mitosis. By rendering light-sheet microscopy operational for high-throughput screening applications, this workflow enables target gene characterization or drug candidate evaluation in tissue-like 3D cell culture models.

Ana Loncar, Sergio A. Rincon, Manuel Lera Ramirez, Anne Paoletti, and Phong T. Tran

To segregate the chromosomes faithfully during cell division, cells assemble a spindle that captures the kinetochores and pulls them towards opposite poles. Proper spindle function requires correct interplay between microtubule motors and non-motor proteins. Defects in spindle assembly or changes in spindle dynamics are associated with diseases like cancer or developmental disorders. Here we compared mitotic and meiotic spindles in fission yeast. We show that even though mitotic and meiotic spindles undergo the typical three phases of spindle elongation, they have distinct features. We found that the relative concentration of kinesin-14 Pkl1 is decreased in meiosis I compared to mitosis, while the concentration of kinesin-5 Cut7 remains constant. We identified the second kinesin-14 Klp2 and microtubule dynamics as factors necessary for proper meiotic spindle assembly. This work defines differences between mitotic and meiotic spindles in fission yeast, and provides prospect for future comparative studies.

The Debye scattering equation (DSE) is generalized and augmented in order to account for moderate texture effects, yielding the differential cross section as a function of atomic coordinates and texture coefficients subject to symmetry constraints. Implications for the evaluation of the pair distribution function (PDF) as a direct transform of powder diffraction data from textured samples are also discussed.

Celo, a competing project to...

Apr 8, 2020; 133:e0701-e0701
RESEARCH HIGHLIGHT

Björn Eismann
Apr 15, 2020; 0:jcs.245043v1-jcs.245043
TOOLS AND RESOURCES

A new global map of urban areas is available for use which shows that cities occupy less than 0.5 per cent of the world’s total land area.

A new study has assessed the annoyance caused by a combination of noises typically found on an industrial site. The results could help improve total noise annoyance prediction models. For example, it was found that ‘broadband’ noises, which consist of a wide range of frequencies, lead to more annoyance if they are combined with a specific additional set of low frequency noises, which can lead to an overall identical noise level.

Hybrid and electric vehicles emit lower levels of carbon dioxide and air pollutants than conventional petrol and diesel vehicles, yet their market uptake in the EU remains limited. New research provides an assessment of the Total Cost of Ownership (TCO) — which combines purchase and operating expenses — of different vehicle types. By comparing historical data on hybrid, petrol and diesel vehicles in three countries (Japan, the UK and the USA), researchers found a strong link between TCO and market share of those vehicles. They also identified a number of ways in which policymakers may promote the adoption of cleaner vehicles through the provision of financial incentives.

Watford moved another point towards Premier League safety after an intriguing lunchtime kick-off against Tottenham Hotspur ended in a 0-0 draw.

Qatar will acquire 30 percent of Total's participating interest in select blocks in the Campeche basin.

Total SA set out bolder commitments to eliminate most of its carbon emissions by 2050, while curbing spending on oil and gas projects due to the crude-price slump.

Williams will provide offshore natural gas transportation services to the Anchor development, thanks to an agreement with co-owners Chevron and Total E&P USA, Inc.

Just try not to think about who — or what — might be watching as you settle in for the night.

Residents of the Big Apple may catch sight of an unusual sight over their famous skyline this week: a NASA space shuttle riding atop a jumbo jet.

Although a recent renewable energy bill stalled, the hurricane-ravaged territory isn't giving up on its fossil fuel-free aspirations.

The European Union has banned bee-harming neonicotinoids. The policy is expected to come into full force by the end of 2018.

The shipping industry embraces fuel efficiency with hybrid delivery fleets.

A new yogitoes bag made of used plastic bottles combines a reusable tote with a yoga mat sling. Reduce waste while greening your yoga practice.

Mark your calendars now for the Great North American Eclipse of April 8, 2024.

Monster-like creatures live thousands of feet below the surface, and they've adapted to hostile environments by taking on cool — and scary — physical traits.

This technology could help Thailand's stray dog population go from zeroes to heroes.

The ABI says insurers are expected to pay £900m towards business interruption claims.

The Total Choice Premier Channel Package from DIRECTV includes practically every channel offered in the United States for a great price.

With millions of car and truck shoppers looking to place their holiday orders, CustomAutoTrim.Com has stepped up all graphic kit productions, stocking the shelves full of Rear Window Graphics before the holiday rush.

Make Donations for the Toys For Tots Charity at all Quality Sleep Mattress Stores

Yellen Partners Has Been Hired to Conduct Liquidation of 60-Year Old Family Business

Blending physical moves from yoga, fitness, martial arts, and ballet with traditional board game play, Equilibrium has resonated with families everywhere looking to unplug and reconnect with each other and with their bodies!

Frances Fuller has followed up her award winning book about Lebanon, In Borrowed Houses, with a new book that helps us face a challenge each and every one of us must someday face.

Season Two Reveals Whether Adrienne's Love Life Will Bring Tricks or Treats

This Summer, on the 29th of June, the Totet Safety Valve will be presented at the ASD Show in Las Vegas. You can find Totet to see this innovative device and find out how the newest Safety Valve can help you protect your Toilet.

Dr. Miklos Toth, M.D, has been honored for his outstanding contributions to the field of Obstetrics and Gynecology as well as co-founding the Millennium Health Foundation, a nonprofit clinic in Uganda, alongside his wife.

Expanded Facility Means Customers Get Faster Service for SLS, SLA, FDM and CNC Projects

While a vehicle service protection plan can not prevent your car from breaking down, it can help cover the costs of the repair and or replacement.

Total Shade Inc., an industry leader with existing patents that reduce heat transfer beyond windows, is set to excite the market again with its latest product launch.

Independent analysis of software rates technology providers across seven product and customer assurance evaluation categories

PARIS (Reuters) - The number of people who have died from coronavirus infections in France rose by 80 to 26,310 on Saturday, the health ministry said, a much smaller daily increase than the previous day when it was 243. The ministry said the number of people in intensive care units - a key measure of a health system's ability to deal with the epidemic - fell by 56, or about 2%, to 2,812. That is less than half the peak of 7,148 seen on April 8

The post France says total death toll from coronavirus rises by 80 to 26,310 appeared first on Firstpost.

Nearly 82 per cent of the total remittances received by the country originated from eight countries - the UAE, US, Saudi Arabia, Qatar, Kuwait, Oman, UK, and Malaysia.

"Thirty new cases of COVID-19 (six from Delhi and 24 from Tripura) have been reported from different establishments. All of them are under the best available medical care at AIIMS-Jhajjar and G B Pant Hospital, Agartala," BSF spokesperson Shubhendu Bhardwaj said in a statement.

A CSS only method of hovering over a horizontal menu link to change the whole menu background image.

A dropline menu using a totally NEW method that requires NO hacks and works in all browsers. Also allows tabbing.

Kalydeco is the first medicine in Europe to treat the underlying cause of the disease

WARNER MUSIC GROUP has released its second-quarter financial results for the period ended MARCH 31, 2020. “We had a tough comparison with an especially strong Q2 in 2019, so I’m … more

Choosing the ultimate theme for your WordPress site is easier said than done. You need something easy to use, attractive, […]

The post 40 Examples of Sites Using the Total Theme appeared first on WPHacks.

Applying the equivariant moving frames method, we construct convergent normal forms for real-analytic 5-dimensional totally nondegenerate CR submanifolds of C^4. These CR manifolds are divided into several biholomorphically inequivalent subclasses, each of which has its own complete normal form. Moreover it is shown that, biholomorphically, Beloshapka's cubic model is the unique member of this class with the maximum possible dimension seven of the corresponding algebra of infinitesimal CR automorphisms. Our results are also useful in the study of biholomorphic equivalence problem between CR manifolds, in question.

Consider that $u_0$ nodes are aware of some piece of data $d_0$. This note derives the expected time required for the data $d_0$ to be disseminated through-out a network of $n$ nodes, when communication between nodes evolves according to a graphical Markov model $overline{ mathcal{G}}_{n,hat{p}}$ with probability parameter $hat{p}$. In this model, an edge between two nodes exists at discrete time $k in mathbb{N}^+$ with probability $hat{p}$ if this edge existed at $k-1$, and with probability $(1-hat{p})$ if this edge did not exist at $k-1$. Each edge is interpreted as a bidirectional communication link over which data between neighbors is shared. The initial communication graph is assumed to be an Erdos-Renyi random graph with parameters $(n,hat{p})$, hence we consider a emph{stationary} Markov model $overline{mathcal{G}}_{n,hat{p}}$. The asymptotic "$u_0$-expected flooding time" of $overline{mathcal{G}}_{n,hat{p}}$ is defined as the expected number of iterations required to transmit the data $d_0$ from $u_0$ nodes to $n$ nodes, in the limit as $n$ approaches infinity. Although most previous results on the asymptotic flooding time in graphical Markov models are either emph{almost sure} or emph{with high probability}, the bounds obtained here are emph{in expectation}. However, our bounds are tighter and can be more complete than previous results.

We deal with various Diophantine equations involving the Euler totient function and various sequences of numbers, including factorials, powers, and Fibonacci sequences.

We prove an inequality between the sum of the Betti numbers of a complex projective manifold and its total curvature, and we characterize the complex projective manifolds whose total curvature is minimal. These results extend the classical theorems of Chern and Lashof to complex projective space.