Mr. Marcho channels years of professional excellence to his work with Sheldon-Kukuchka Funeral Home, Inc.

The Alabama Supreme Court issued a writ of mandamus compelling a trial court to dismiss the breach-of-contract and bad-faith claims against an insurance carrier arising out of a roofing worker’s…

An Ohio appellate court issued a limited writ of mandamus directing the Industrial Commission to determine a worker’s entitlement to temporary total disability benefits for a period of unemployment. Case: State…

Cloud provider allegedly approached by Google ratted out shady group to Microsoft.

The Spanish government will propose to the European Commission to amend the post-COVID reconstruction plan to redirect unused EU funds to the 'reconstruction and revitalisation' of the areas most affected by the recent torrential rains and floods. ‘We are going to present an addendum to the…

“Close Encounters: Facing the Bomb in a New Nuclear Age” is on display at SPIA’s Bernstein Gallery through Oct. 25. 

Programmed ribosomal frameshifting (PRF) is a mechanism used by arteriviruses like porcine reproductive and respiratory syndrome virus (PRRSV) to generate multiple proteins from overlapping reading frames within its RNA genome. PRRSV employs −1 PRF directed by RNA secondary and tertiary structures within its viral genome (canonical PRF), as well as a noncanonical −1 and −2 PRF that are stimulated by the interactions of PRRSV nonstructural protein 1β (nsp1β) and host protein poly(C)-binding protein (PCBP) 1 or 2 with the viral genome. Together, nsp1β and one of the PCBPs act as transactivators that bind a C-rich motif near the shift site to stimulate −1 and −2 PRF, thereby enabling the ribosome to generate two frameshift products that are implicated in viral immune evasion. How nsp1β and PCBP associate with the viral RNA genome remains unclear. Here, we describe the purification of the nsp1β:PCBP2:viral RNA complex on a scale sufficient for structural analysis using small-angle X-ray scattering and stochiometric analysis by analytical ultracentrifugation. The proteins associate with the RNA C-rich motif as a 1:1:1 complex. The monomeric form of nsp1β within the complex differs from previously reported homodimer identified by X-ray crystallography. Functional analysis of the complex via mutational analysis combined with RNA-binding assays and cell-based frameshifting reporter assays reveal a number of key residues within nsp1β and PCBP2 that are involved in complex formation and function. Our results suggest that nsp1β and PCBP2 both interact directly with viral RNA during formation of the complex to coordinate this unusual PRF mechanism.

Jenni Farley discusses her directorial debut "Devon," streaming and on video-on-demand Tuesday, and how she applied her love of horror movies to making one.

'It must have been the nervous excitement of shooting with THE Amitabh Bachchan. I had kept tossing and turning all night. The next morning, I promptly fainted in my choreographer Farah Khan's arms. When I came round, I heard Amitji saying, 'Please don't faint. I promise I'll dance well.''

Filmmaker Stella Meghie says it is a "privilege" to direct the Whitney Houston biopic, "I Wanna Dance with Somebody". The "Photograph" director said it was important for her to tell Whitney's story and "extend" the music icon's legacy. "Whitney Houston is the greatest singer of all time, so it's a privilege to be able to tell her story. I'm very lucky to have the support of Pat Houston (Whitney's sister-in-law) and Anthony McCarten (screenwriter) and Clive Davis (music producer) to direct the film. "It's amazing talking about her so much and it's just very important to all of us to kind of extend her legacy and to tell her story. It's the most exciting job I've ever been in a position to tell," Meghie told The Hollywood Reporter. Davis, who mentored the late singer, is producing the project along with the Whitney Houston estate and McCarten. Whitney died in 2012 aged 48, drowning in her bathtub after ingesting a large amount of cocaine. Despite a career marked by drug addiction, she ..

Randamoozham, the Mohanlal starring period drama is one of the most anticipated upcoming films of the Indian film industry. Recently, it was rumoured that Priyadarshan, the senior filmmaker might take over the Mohanlal project as the director. The rumours started doing

Please put away any initial thoughts of the way you think a real movie director should sound and act doing their job. It's tough not to want to emulate directors you respect both in style and attitude.

Mission agency releases new report, challenges people everywhere to 'help end extreme poverty in this generation'

The presently-disclosed subject matter relates to compounds of the formula: and methods for use thereof. The presently-disclosed subject matter relates methods of selectively differentiating a stem cell, and methods of screening for compounds useful for enhancing terminal differentiation of committed cardiac progenitor cells.

The invention relates to a method for producing implants and components by direct shaping. The method includes the steps of producing a mold for the implants or components to be produced, making allowances for changes in geometry occurring during after-treatment after the implants or components are removed from the mold, and, providing partial areas of the mold with a structured surface for transferring this structure to the surfaces of the implants or components. In the method, raw material is introduced into the mold and, after remaining in the mold for a certain period of time, the hardened raw material is then removed from the mold to allow the implants or components to take on the structured surface of the mold as well as its geometry. The demolded implants or components are finally subjected to any after-treatment that may be necessary.

A guide has an open end and a finger structure preferably of a shape memory alloy. The guide is run in small-diameter configuration through a restriction with a fishing tool, such as an overshot, above it. Once through the restriction, power to heaters on the fingers takes the material past its transition temperature to allow the guide lower end to fan out and surround a skewed fish that is in a slanted position and leaning on a wall of a surrounding tubular that has a larger dimension than the restriction. The bottomhole assembly is then advanced until the fish is captured by the fishing tool and pulled out of the hole. The fingers are forcibly retracted as the assembly is pulled back through the restriction. The guide can use retained fingers with an outward bias to flare out after passing through a restriction, thus acting as a fish guide.

The six direction directing device includes a shaft, a rotation member, a driving source, and a guide member. The shaft extends in an X axis direction. The rotation member is rotatable around an inclined axis inclined at α deg with respect to the shaft. The rotation member has an xyz rectangular coordinate system. The rotation member further includes a spherical surface and an orbit portion formed around an x axis of the spherical surface. The guide member is fixed to an XYZ rectangular coordinate system. The orbit portion has such a shape that the y axis of the rotation member is sequentially directed in + and − directions on the U, V, and W axes that cross one another at intervals of 60 deg around the X axis when the rotation member is rotated in contact with the guide member by the rotation of the shaft.

Police oppose bail for an IT expert accused of directing the importation of more than a billion dollars' worth of drugs into Australia, after prosecutors alleged he was part of a sophisticated plan of cyber coverup and stolen identities.

The "Felicity" alum talks about her own real-life parallels to the film, directing Huffman on set and why she’s left acting behind permanently.

The owner of a Louisiana pharmacy pleaded guilty today for directing a $2.2 million Medicare fraud scheme to repackage and redistribute prescription medications. Mona Patrice Carter, 47, pleaded guilty before U.S. District Judge James J. Brady of the Middle District of Louisiana to one count of health care fraud.

Alli McCartney of UBS Private Wealth Management and Darrell Cronk of Wells Fargo Investment Institute join "Squawk on the Street" to discuss the markets and investing amid the coronavirus pandemic.

15 years after Friday Night Lights first hit the big screen, Universal Pictures is moving forward with a new adaptation.

After nearly three decades since hitting theaters, Disney+ has confirmed their plans to move forward with a sequel to the cult Halloween movie, Hocus Pocus on Wednesday.

Disney fired the 52-year-old filmmaker in July after offensive tweets he wrote between 2008-2012 joking about rape, child abuse, and pedophilia

Channing Tatum is slated to make his directorial debut with a heartfelt and personal story, Dog, based on his beloved pooch Lulu who passed away in December 2018.

Randamoozham, the Mohanlal starring period drama is one of the most anticipated upcoming films of the Indian film industry. Recently, it was rumoured that Priyadarshan, the senior filmmaker might take over the Mohanlal project as the director. The rumours started doing

Innes, Christopher, 1941- author

The Supreme Court stayed the order after the Centre said it was ‘unworkable’ and passed without consultation.

Rotch Library - DS625.T73 2017