Single-particle electron cryo-microscopy (cryoEM) has undergone a `resolution revolution' that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expression and purification of macromolecular complexes need to be explored. Macromolecular assemblies are often unstable, and invasive construct design or inadequate purification conditions and sample-preparation methods can result in disassembly or denaturation. The structure of the 2.6 MDa yeast fatty acid synthase (FAS) has been studied by electron microscopy since the 1960s. Here, a new, streamlined protocol for the rapid production of purified yeast FAS for structure determination by high-resolution cryoEM is reported. Together with a companion protocol for preparing cryoEM specimens on a hydrophilized graphene layer, the new protocol yielded a 3.1 Å resolution map of yeast FAS from 15 000 automatically picked particles within a day. The high map quality enabled a complete atomic model of an intact fungal FAS to be built.

Engineers shouldn’t have to reinvent the wheel—or wings, or sonar systems—when mother nature has already done much of the heavy design work. In fact, finding […]

The post Next Engineering Revolution Is Hiding in Museum Collections appeared first on Smithsonian Insider.

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Carmakers have been quietly delivering significant cuts in CO2 emissions with technology advances, weight reduction and aerodynamic improvements.

Japan's plan to wean off nuclear power could help lead a global push toward cleaner, more efficient civilization, says U.S. energy guru Amory Lovins.

Journalist Heather Rogers maintains that we can't buy our way out of the crisis our planet is experiencing.

In a new world of digital fabrication, computer-driven tools of all kinds will change the way we make things.

Big money is being poured into a technology that could make it possible to modify your DNA.

New designs like the Eargo and new rules from the government may change everything.

Kids in England have been raising money to fund solar for schools in Africa, but now they are bringing it closer to home too.

How do these global cities stack up in being green?

A protein complex has been shown to repair DNA damage caused by radiation or old age.

April 24th is the anniversary of the Rana Plaza garment factory collapse; Fashion Revolution Day wants to change how our clothes are made.

Shared mobile health app will allow doctors to monitor a patient's health in real-time — and intervene before a hospital visit is needed.

Stephen Ritz, author of 'The Power of a Plant,' developed a curriculum for indoor gardening that is changing lives and improving schools.

Follow the proceedings of the Clean Energy Summit via Twitter #NCES09.

The disruption ahead extends well beyond healthcare. Are you prepared?

Mathieu Flamini has been keeping a secret: He's developing a viable alternative to oil.

Other countries have already installed thousands of offshore turbines. Will the U.S. be next?

SEO Elite claims that it can help you with search engine optimization in such a way as to increase your site's visibility in the major search engines. Is this a useful product, or just another scam designed to take your money?

This article explains how the search engine became to be what it is today and how it changed our lives.

Creators Launch Crowd funding Campaign to Fund Manufacturing and Distribution Cost of Their Redesigned Folding E-Bike

Sciacallo Bikes Direct To Consumer Model Is Winning Customers One Bike At A Time

Founder Dr. K.P. Yohannan says 'so much more yet to do' as missions agency celebrated their anniversary July 3

Damian Sanders, one of the true pioneers of professional snowboarding, will soon release his revolutionary fitness product on Kickstarter, the XBAR.

Minnesota's Newest Tier 1 hockey team helps launch top girls players on to future hockey and academic success

Nine housing projects are currently being developed: five in the East Coast, two in the West Coast and another two – in Roseau City. The scheme was made possible thanks to the small island's successful Citizenship by Investment (CBI) Programme.

Mezator combined technology solutions they had already implemented and the IoT approach, added a dedicated, intuitive app and created a #selfieready device.

Healthcare Pros Cite ConnectVitals RPM Platform as Vital to Improving Patient Care, Decreasing Readmissions, and Reducing Costs

New patented Technology outperforms leader's "heat and cool air purifier" system and all others.

What are exosomes?

The book became immediately bestseller on Amazon and also many other bookstores

Finally - a big screen laser cinema projector that ACTUALLY fits in your pocket. With standalone and Raspberry Pi compatible versions.

Cool Shield Releases New MagSeal Line of Products to Increase the Standards For Sealing Airflow Gaps in Data Center Aisle Containment

During SEMA 2019 MSS are on a mission to revolutionise the way you think about suspension.

TruClean stops the growth of 99.999% of bacteria & viruses and controls microbial growth for up to 12 months and – has been applied to every surface of the casino, hotel and gas station

Local Technology Company Revolutionizes IT Support by Launching Managed Workplace Services

Designer Sportswear Company from Chicago "D2 Wear" has teamed up with FashionBar for a sportswear apparel fashion show on July 28th at 3:00 pm at the Northbrook mall, Illinois; Admission is free for visitors.

How to Lose Weight and Actually Keep It Off

There is a one in three chance that a motorist will require roadside assistance in the next 12 months due to battery failure or a related electrical system issue.

RideClean is an all-in-one premium ultra polisher & sealer and it's made in the USA.

Scott Cook, cofounder and chairman of the executive committee at Intuit.

The founder of the Khan Academy talks with HBR senior editor Alison Beard.

Think about many of the technologies we now take for granted, like the smartphone. Think about how significantly the smartphone has changed the way we live and work. Think about how your business life was a mere 10-12 years ago.… Read More

The post Blockchain: A Quiet Revolution? A Disruptor? Both? appeared first on Anders CPAs.

For decades, organizations have tried to unlock the collective knowledge contained within their people and systems. And the challenge is getting harder, since every year, massive amounts of additional information are created for people to share. We've reached a point at which individuals are unable consume, understand, or even find half the information that is available to them.

As India becomes digital-savvy, consumer dynamics have evolved more rapidly than any of us imagined.

The procedure, which has only been conducted a few times in history, could be a groundbreaking new treatment for patients suffering from lung cancer.

The post In a Revolutionary Medical Treatment, Man’s Lung Was Removed, Cleaned, and Replaced—No Transplant Necessary appeared first on Good News Network.