This afternoon President Trump ordered American flags at all U.S. government buildings and facilities be flown at half-staff beginning immediately until sunset on May 22, as a mark of solemn respect for the victims of the shootings at Santa Fe High School in Texas. In concurrence with the President’s order and as an expression of […]

This morning President Trump ordered American flags at all U.S. government buildings and facilities be flown at half-staff beginning immediately until sunset today , as a mark of solemn respect for the victims of the terrible act of violence perpetrated on June 28, 2018 in Annapolis MD. In concurrence with the President’s order and as […]

Governor Carney has ordered the United States and Delaware flags at state buildings and facilities be flown at half-staff beginning today, July 19 to mark the passing of former Delaware Representative Katharine Jester. Representative Jester served the residents of the Middletown area  and was elected to the House of Representatives in 1980 and served until […]

Senator John McCain proudly served his country as a Navy aviator during the Vietnam War and as a statesman representing the State of Arizona in the U.S. House and Senate for over thirty years. In remembrance of the life and service of Senator McCain, Governor Carney has ordered the U.S. and Delaware flags to half […]

By a joint resolution approved 12/18/2001, (Public Law 107-89) September 11th of each year has been designated as “Patriot Day”. This law also directs the flags be lowered to half-staff for the entire day on September 11th. In concurrence with this directive, Governor Carney has ordered the U. S. and Delaware flags be flown at […]

Late last evening, President Trump ordered American flags at all U.S. government buildings and facilities to be flown at half-staff until sunset on October 31, 2018 as a mark of solemn respect for the victims of the senseless act of violence at the Tree of Life synagogue in Pittsburgh. In concurrence with the President’s order […]

This morning President Trump ordered American flags at all U.S. government buildings and facilities to be flown at half-staff until sunset on November 10, 2018 as a mark of solemn respect for the victims of the terrible act of violence perpetrated in Thousand Oaks, California, on November 7, 2018. In concurrence with the President’s order […]

With the passing of former President George H. W. Bush on November 30, 2018 President Trump has ordered United States flags at all government buildings and facilities be flown at half-staff beginning immediately in honor and tribute to the memory of President George H.W. Bush, and as an expression of public sorrow. In concurrence with […]

With the passing of former State Senator Steven Amick on Wednesday, January 23 Governor Carney has ordered the United States and Delaware flags at state buildings and facilities be flown at half-staff beginning Friday morning, February 1 as a mark of respect for Mr. Amick and his service to Delaware. Mr. Amick’s legislative career began […]

Governor John Carney today ordered United States and Delaware flags lowered to half-staff in honor of U.S. Marines Staff Sergeant Christopher Slutman, who was killed in action in Afghanistan on Monday. Staff Sergeant Slutman resided in Delaware along with his family. Flags are to be returned to full staff Monday morning.     Click here […]

Wednesday, May 15 is Peace Officers Memorial Day. On this day we pay tribute to peace officers across our country who have died, or who have been disabled in the line of duty. In accordance with federal law (36 U.S.C. 175) and a proclamation issued by President Trump, Governor Carney requests that the Delaware and […]

On Memorial Day, we pay tribute to those who have died in service upholding the freedoms that all Americans enjoy today. To honor the dedication and service of all of our service members Governor Carney requests that the proper protocol for the U.S. Flag Code be followed this Memorial Day. Flags are to be lowered […]

This afternoon President Trump ordered American flags at all U.S. government buildings and facilities to be flown at half-staff until sunset on Tuesday, June 4 as a mark of solemn respect for the victims of the terrible act of violence perpetrated in Virginia Beach, Virginia on May 31, 2019. In concurrence with the President’s order […]

Governor Carney has ordered the U.S. and Delaware flags lowered to half staff beginning the morning of Friday June 7 through Monday morning June 10 in remembrance of former State Representative Robert Powell and former Delaware State University President Dr. William DeLauder. Mr. Powell was first elected to the Delaware General Assembly in November 1972 […]

With the passing of former U.S. Supreme Court Justice John Paul Stevens, President Trump has ordered U.S. flags at all government buildings and facilities be flown at half-staff the day of his interment, Tuesday, July 23rd as a mark of respect for the memory of Justice Stevens and to honor his service to our country. […]

Today President Trump ordered American flags at all U.S. government buildings and facilities to be flown at half-staff until sunset on August 8, 2019 as a mark of solemn respect for the victims of the terrible acts of violence perpetrated in El Paso, Texas on August 3, 2019 and in Dayton, Ohio on August 4, […]

By a joint resolution approved 12/18/2001, (Public Law 107-89) September 11th of each year has been designated as “Patriot Day”. This law also directs the flags be lowered to half-staff for the entire day on September 11th. In concurrence with this directive, Governor Carney has ordered the U. S. and Delaware flags be flown at […]

Mr. Sullivan was appointed as the Chief Public Defender in 1970, serving 39 years when he retired in 2009. Under his direction, the Office of the Public Defender grew from 10 employees to a full-service law firm employing nearly 150 people and 70 attorneys.

Previous guidance issued this morning provided for the U.S. and Delaware flags to be flown at half staff Friday, September 20 to mark the passing of former Public Defender Larry Sullivan. Title 29, Section 408 of the Delaware Code requires Delaware State agencies, including all public schools, to display a POW/MIA flag on National POW/MIA […]

Everyday across Delaware, thousands of firefighters serve their communities and protect the public by responding to not only fires but almost any emergency situation. Sunday, October 6 has been recognized by Congress as the day US and State flags are to be flown at half-staff in recognition of the National Fallen Firefighters Memorial Service.

Gov. Carney ordered the United States and Delaware flags at state buildings be flown at half-staff. Flags are to be returned to full staff at the close of business on Friday, October 18.

Each year, on December 7 we honor the over 2,400 men and women who lost their lives for our country on this day in 1941.

Services for Mr. Haskell are scheduled for 10 AM on Monday January 27. As such, flags are to be lowered Monday morning January 27 and are to be returned to full-staff at the close of business on Monday, January 27.

With the passing of former State Representative William “Bobby” Outten on Tuesday, March 17 Governor Carney has ordered the United States and Delaware flags at state buildings and facilities be flown at half-staff beginning today, March 20 as a mark of respect for Mr. Outten and his service to Delaware. Mr. Outten was elected to […]

Deccan Odyssey, one of the earliest luxury trains in India, has been flagged off by the Maharashtra Tourism Development Corporation (MTDC) in a completely new look for this season’s run. The train now has refurbished interiors and includes a spa, fitness ceter, lounge with library, beauty...

Social media feeds are filled with historic shots marking Victory Day, but Facebook seems to have taken issue with one that symbolizes the Soviet victory over Nazi Germany, and it keeps deleting a recently-colorized version of it.
Read Full Article at RT.com

The MHA had issued an advisory for Zoom users in India in April, to safeguard their virtual meetings from prying eyes, deeming the video conferencing platform unsafe.

When it comes to flagship chipsets for Android phones, Qualcomm is typically regarded as being the top contender with its 800-series processors. That being said, MediaTek has been playing an incredible catchup game as of late with the Dimensity 1000 in late 2019 which put it within a striking range of the new Snapdragon 865 ...

Youtube Video on Hoisting of World's Largest and Tallest Tiranga - Indian National Tricolour Flag

Grub2 has grub2-set-bootflag setuid in the new Fedora release and has the ability to corrupt the environment.

On Wednesday in New Jersey, the world’s largest regional theme park company and the largest operator of waterparks in North America said that its New Jersey park, Six Flags Great Adventure, is now powered by solar energy.

Rawalpindi : The Rawalpindi Police with assistance of other security institutions staged a flag march in the city to create awareness among public on prevailing alarming situation due to coronavirus pandemic and preventive measures adopted by the Punjab government.According to the police...

ABSTRACT

Bacterial flagella are rotating nanomachines required for motility. Flagellar gene expression and protein secretion are coordinated for efficient flagellar biogenesis. Polar flagellates, unlike peritrichous bacteria, commonly order flagellar rod and hook gene transcription as a separate step after production of the MS ring, C ring, and flagellar type III secretion system (fT3SS) core proteins that form a competent fT3SS. Conserved regulatory mechanisms in diverse polar flagellates to create this polar flagellar transcriptional program have not been thoroughly assimilated. Using in silico and genetic analyses and our previous findings in Campylobacter jejuni as a foundation, we observed a large subset of Gram-negative bacteria with the FlhF/FlhG regulatory system for polar flagellation to possess flagellum-associated two-component signal transduction systems (TCSs). We present data supporting a general theme in polar flagellates whereby MS ring, rotor, and fT3SS proteins contribute to a regulatory checkpoint during polar flagellar biogenesis. We demonstrate that Vibrio cholerae and Pseudomonas aeruginosa require the formation of this regulatory checkpoint for the TCSs to directly activate subsequent rod and hook gene transcription, which are hallmarks of the polar flagellar transcriptional program. By reprogramming transcription in V. cholerae to more closely follow the peritrichous flagellar transcriptional program, we discovered a link between the polar flagellar transcription program and the activity of FlhF/FlhG flagellar biogenesis regulators in which the transcriptional program allows polar flagellates to continue to produce flagella for motility when FlhF or FlhG activity may be altered. Our findings integrate flagellar transcriptional and biogenesis regulatory processes involved in polar flagellation in many species.

IMPORTANCE Relative to peritrichous bacteria, polar flagellates possess regulatory systems that order flagellar gene transcription differently and produce flagella in specific numbers only at poles. How transcriptional and flagellar biogenesis regulatory systems are interlinked to promote the correct synthesis of polar flagella in diverse species has largely been unexplored. We found evidence for many Gram-negative polar flagellates encoding two-component signal transduction systems with activity linked to the formation of flagellar type III secretion systems to enable production of flagellar rod and hook proteins at a discrete, subsequent stage during flagellar assembly. This polar flagellar transcriptional program assists, in some manner, the FlhF/FlhG flagellar biogenesis regulatory system, which forms specific flagellation patterns in polar flagellates in maintaining flagellation and motility when activity of FlhF or FlhG might be altered. Our work provides insight into the multiple regulatory processes required for polar flagellation.

ABSTRACT

The cell cycle is a critical component of cellular proliferation, differentiation, and response to stress, yet its role in the regulation of intracellular symbioses is not well understood. To explore host-symbiont cell cycle coordination in a marine symbiosis, we employed a model for coral-dinoflagellate associations: the tropical sea anemone Aiptasia (Exaiptasia pallida) and its native microalgal photosymbionts (Breviolum minutum and Breviolum psygmophilum). Using fluorescent labeling and spatial point-pattern image analyses to characterize cell population distributions in both partners, we developed protocols that are tailored to the three-dimensional cellular landscape of a symbiotic sea anemone tentacle. Introducing cultured symbiont cells to symbiont-free adult hosts increased overall host cell proliferation rates. The acceleration occurred predominantly in the symbiont-containing gastrodermis near clusters of symbionts but was also observed in symbiont-free epidermal tissue layers, indicating that the presence of symbionts contributes to elevated proliferation rates in the entire host during colonization. Symbiont cell cycle progression differed between cultured algae and those residing within hosts; the endosymbiotic state resulted in increased S-phase but decreased G₂/M-phase symbiont populations. These phenotypes and the deceleration of cell cycle progression varied with symbiont identity and host nutritional status. These results demonstrate that host and symbiont cells have substantial and species-specific effects on the proliferation rates of their mutualistic partners. This is the first empirical evidence to support species-specific regulation of the symbiont cell cycle within a single cnidarian-dinoflagellate association; similar regulatory mechanisms likely govern interpartner coordination in other coral-algal symbioses and shape their ecophysiological responses to a changing climate.

IMPORTANCE Biomass regulation is critical to the overall health of cnidarian-dinoflagellate symbioses. Despite the central role of the cell cycle in the growth and proliferation of cnidarian host cells and dinoflagellate symbionts, there are few studies that have examined the potential for host-symbiont coregulation. This study provides evidence for the acceleration of host cell proliferation when in local proximity to clusters of symbionts within cnidarian tentacles. The findings suggest that symbionts augment the cell cycle of not only their enveloping host cells but also neighboring cells in the epidermis and gastrodermis. This provides a possible mechanism for rapid colonization of cnidarian tissues. In addition, the cell cycles of symbionts differed depending on nutritional regime, symbiotic state, and species identity. The responses of cell cycle profiles to these different factors implicate a role for species-specific regulation of symbiont cell cycles within host cnidarian tissues.

ABSTRACT

Bacterial flagellar motility plays an important role in many processes that occur at surfaces or in hydrogels, including adhesion, biofilm formation, and bacterium-host interactions. Consequently, expression of flagellar genes, as well as genes involved in biofilm formation and virulence, can be regulated by the surface contact. In a few bacterial species, flagella themselves are known to serve as mechanosensors, where an increased load on flagella experienced during surface contact or swimming in viscous media controls gene expression. In this study, we show that gene regulation by motility-dependent mechanosensing is common among pathogenic Escherichia coli strains. This regulatory mechanism requires flagellar rotation, and it enables pathogenic E. coli to repress flagellar genes at low loads in liquid culture, while activating motility in porous medium (soft agar) or upon surface contact. It also controls several other cellular functions, including metabolism and signaling. The mechanosensing response in pathogenic E. coli depends on the negative regulator of motility, RflP (YdiV), which inhibits basal expression of flagellar genes in liquid. While no conditional inhibition of flagellar gene expression in liquid and therefore no upregulation in porous medium was observed in the wild-type commensal or laboratory strains of E. coli, mechanosensitive regulation could be recovered by overexpression of RflP in the laboratory strain. We hypothesize that this conditional activation of flagellar genes in pathogenic E. coli reflects adaptation to the dual role played by flagella and motility during infection.

IMPORTANCE Flagella and motility are widespread virulence factors among pathogenic bacteria. Motility enhances the initial host colonization, but the flagellum is a major antigen targeted by the host immune system. Here, we demonstrate that pathogenic E. coli strains employ a mechanosensory function of the flagellar motor to activate flagellar expression under high loads, while repressing it in liquid culture. We hypothesize that this mechanism allows pathogenic E. coli to regulate its motility dependent on the stage of infection, activating flagellar expression upon initial contact with the host epithelium, when motility is beneficial, but reducing it within the host to delay the immune response.

While flagella have been studied extensively as motility organelles, with a focus on internal structures such as the axoneme, more recent research has illuminated the roles of the flagellar surface in a variety of biological processes. Parasitic protists of the order Kinetoplastida, which include trypanosomes and Leishmania species, provide a paradigm for probing the role of flagella in host-microbe interactions and illustrate that this interface between the flagellar surface and the host is of paramount importance. An increasing body of knowledge indicates that the flagellar membrane serves a multitude of functions at this interface: attachment of parasites to tissues within insect vectors, close interactions with intracellular organelles of vertebrate cells, transactions between flagella from different parasites, junctions between the flagella and the parasite cell body, emergence of nanotubes and exosomes from the parasite directed to either host or microbial targets, immune evasion, and sensing of the extracellular milieu. Recent whole-organelle or genome-wide studies have begun to identify protein components of the flagellar surface that must mediate these diverse host-parasite interactions. The increasing corpus of knowledge on kinetoplastid flagella will likely prove illuminating for other flagellated or ciliated pathogens as well.

The green-and-white banner from an Illinois high school recalls the first Earth Day 50 years ago

Police have been alerted to more than 1,000 potential child sexual abuse cases during the coronavirus pandemic, the Home Secretary has said.

Gareth Bale has spoken for the first time about the 'Wales. Golf. Madrid' flag celebration which upset Real Madrid fans last year.

Local businesses forced to close by the pandemic are offered a glimmer of hope, as the WA Government flags the state could be more "economically progressive" than others due to its low COVID-19 case numbers.

Australian shares drop as US oil plunges to a 21-year low, Caltex takeover fails due to coronavirus risk, and NAB flags $1.1 billion hit to its earnings.

He has claimed almost every individual honour there is in the AFL, but the highest goal continues to drive the Geelong star even during this uncertain 2020 season — a maiden premiership victory.