Many transferable quinolone-resistance mechanisms have been already identified in Gram-negative bacteria. The plasmid-encoded 65 amino-acid long ciprofloxacin-modifying enzyme, namely CrpP, was recently identified in Pseudomonas aeruginosa. We analyzed a collection of 100 clonally-unrelated and multidrug-resistant P. aeruginosa clinical isolates among which 46 (46%) were found positive for crpP-like genes, encoding five CrpP variants conferring variable levels of reduced susceptibility to fluoroquinolones. Those crpP-like genes were chromosomally located, as part of PAGI-like pathogenicity genomic islands.

As resistance to artemisinins (current frontline drugs in malaria treatment) emerges in south East Asia, there is an urgent need to identify the genetic determinants and understand the molecular mechanisms underpinning such resistance. Such insights could lead to prospective interventions to contain resistance and prevent the eventual spread to other malaria endemic regions. Artemisinin reduced susceptibility in South East Asia (SEA) has been primarily linked to mutations in P. falciparum Kelch-13, which is currently widely recognised as a molecular marker of artemisinin resistance. However, 2 mutations in a ubiquitin hydrolase, UBP-1, have been previously associated with artemisinin reduced susceptibility in a rodent model of malaria and some cases of UBP-1 mutation variants associating with artemisinin treatment failure have been reported in Africa and SEA. In this study, we have employed CRISPR-Cas9 genome editing and pre-emptive drug pressures to test these artemisinin susceptibility associated mutations in UBP-1 in P. berghei sensitive lines in vivo. Using these approaches, we have shown that the V2721F UBP-1 mutation results in reduced artemisinin susceptibility, while the V2752F mutation results in resistance to chloroquine and moderately impacts tolerance to artemisinins. Genetic reversal of the V2752F mutation restored chloroquine sensitivity in these mutant lines while simultaneous introduction of both mutations could not be achieved and appears to be lethal. Interestingly, these mutations carry a detrimental growth defect, which would possibly explain their lack of expansion in natural infection settings. Our work has provided independent experimental evidence on the role of UBP-1 in modulating parasite responses to artemisinin and chloroquine under in vivo conditions.

The off-label use of third generation cephalosporin (3GC) during in ovo vaccination or vaccination of newly hatched chicks, was a common practice worldwide. CMY-2-producing Escherichia coli have been disseminated among broiler production. The objectives of this study were to determine the epidemiological linkage of bla_CMY-2-positive plasmids among broilers both within and outside Japan because grandparent stock and parent stock were imported in Japan. We examined the whole genome sequences of 132 3GC-resistant E. coli isolates collected from healthy broilers during 2002-2014. The predominant 3GC-resistance gene was bla_CMY-2, which was detected in the plasmids of 87 (65.9%) isolates. The main plasmid replicon types were IncI1-I (n=21; 24.1%), IncI (n=12; 13.8%), IncB/O/K/Z (n=28; 32.2%), and IncC (n=22; 25.3%). Those plasmids were subjected to gene clustering and network analyses and plasmid multi-locus sequence typing (pMLST). The chromosomal DNA of isolates was subjected to MLST and single nucleotide variant (SNV)-based phylogenetic analysis.

MLST and SNV-based phylogenetic analysis revealed high diversity of E. coli isolates. ST429 harboring bla_CMY-2-positive IncB/O/K/Z was closely related to isolates from broiler in Germany harboring bla_CMY-2-positive IncB/O/K/Z. pST55-IncI and pST12-IncI1-I and pST3-IncC were prevalent in western Japan. pST12-IncI1-I and pST3-IncC were closely related to those detected in E. coli isolates from chicken in American continent, whereas 26 IncB/O/K/Z were related to those in Europe. These data will be useful to reveal the whole picture of transmission of CMY-2-producing bacteria in and out of Japan.

BACKGROUND:

In the United States, transgender youth are at especially high risk for HIV infection. Literature regarding HIV prevention strategies for this vulnerable, often-hidden population is scant. Before effective, population-based HIV prevention strategies may be adequately developed, it is necessary to first enhance the contextual understanding of transgender youth HIV risk and experiences with HIV preventive services.

Penn State University Libraries has developed a curated guide to COVID-19 related resources for researchers, including ongoing research at Penn State.

Google Translate is not the only app that can help you converse with someone speaking a different language. Here's how to translate real-time conversations with the Google Assistant.

The company also announced a second Walkman, a pair of headphones, and an amplifier.

Christine Yoshinaga-Itano
Nov 1, 1998; 102:1161-1171
ARTICLES

Barbara J. Stoll
Aug 1, 2002; 110:285-291
ARTICLES

See who is through after October's mini-tournaments; the remaining groups run from 13 to 19 November.

A Penn State Abington faculty member is teaching students enrolled to integrate the content into their daily lives to help them manage the impact of the coronavirus.

Linda and Clifton Merchant met at Penn State Behrend and were married for 58 years. A new endowed legacy fund honors Linda Merchant and her time at the college.

Penn State alumnus and author Paul Levine's latest novel, "Cheater's Game," focuses on the college admissions scandal.

The coronavirus death toll continues to rapidly climb in the U.S. and other parts of the world.

Following a March 19 announcement by Pennsylvania Gov. Tom Wolf to end physical operations at many businesses statewide, Penn State Executive Vice President and Provost Nicholas P. Jones shared the following message with the University community.

The International Crisis Group congratulates the members of the Tunisian National Dialogue Quartet as this year’s recipient of the Nobel Peace Prize. It is an apt recognition of its achievement in allowing the spirit of inclusion and compromise to triumph over the polarisation and violence that has been all too prevalent in the region, and of the central role civil society can play at moments of crisis.

(As of 29 April 2020) Information on travel restrictions and advice on quarantine measures.

Single women dominated property sales in 2017, 2018 and 2019, compared to single men and even married couples, according to data analytics firm Lightstone.

The pharmaceutical retailer says it was forced to increase prices of surgical masks because of suppliers' inflated quotes and to match competitors, while the commission says retailers could have been following Dis-Chem's lead.

Mark Alpert made a generous gift $200,000 in memory of his late wife, Claire, to establish the Mark E. and Claire L. Alpert Graduate Fellowship in the Department of Civil and Environmental Engineering at Penn State.

Fly your DJI drone using a first-person perspective up to a range of 2.5 miles and race with up to seven other drones using the same low-latency system.

Preschool-age children who frequently play number-related games at home show better math skills and growth by the end of kindergarten, finds a new study in the journal Child Development.

Bank Audi proposed using government assets as collateral to offset the losses of the Central Bank and commercial banks that were the result of lending to the state.

“Who Did In the Delaware Ducks?” and “Mysteries of History” to be featured

For the fourth year in a row, the Delaware Department of State has received a State Trade and Export Promotion (STEP) award from the U.S. Small Business Administration (SBA.) This year’s award totals $268,096 and will be used to support the state’s efforts to assist small Delaware companies in entering and developing markets overseas.

The latest promos of the ongoing Bigg Boss 13 show reveal that Rashami Desai's alleged boyfriend Arhaan Khan who entered as wild card contestant along with Hindustani Bhau(Vikas Fhatak), Khesari Lal Yadav, Shefali Jariwala, Himanshi Khurana and Tehseen Poonawalla, has already challenged the thick jodi of BB 13, Sidharth Shukla

Delhi: BJP leader Vijay Goel who left his house in an odd numbered car to protest against Odd-Even scheme, issued a challan for violation of the scheme. BJP leader Vijay Goel drove an odd numbered car in protest against Odd-Even scheme, and said, 'This scheme is just a gimmick, they(Delhi

Billionaire Mukesh Ambani just sealed three deals in as many weeks to raise a combined $8 billion, including from Facebook Inc.

State of Delaware offices will open at 10 a.m. on Friday, March 6, 2015. Non-essential employees do not report until 10 a.m. Essential employees are to report as scheduled. More Information

State of Delaware offices in all three counties will open at 10 a.m. on Monday, January 25, 2016. Non-essential employees do not report until 10 a.m. Essential employees are to report as scheduled.

Delaware farmers, lawn care companies, golf courses and other nutrient handlers should not apply nutrients to the ground during the winter months, the Delaware Department of Agriculture says in a reminder.

Delaware farmers, lawn care companies, golf courses and other nutrient handlers should not apply nutrients to the ground during the winter months, the Delaware Department of Agriculture reminds applicators.

 Settlement and Default Handling Procedures in Multilateral and Deferred Net Settlement Systems under the PSS Act, 2007

COVID-19 is truly a global health issue affecting everyone and causing concern for you, our customers. As we all continue to navigate the uncertainty of this outbreak, SAS is committed to supporting your business as a valued partner and will ensure continuity of service. We created this guide to address [...]

Frequently asked questions related to COVID-19 and SAS customer support was published on SAS Users.

Statewide flu totals in Delaware decreased for the third week in a row, according to the Division of Public Health (DPH). During the week ending March 10, DPH reports 290 laboratory-confirmed flu cases, down from the prior week’s total of 381 confirmed cases. The latest numbers increase the total number of influenza cases for the season to 7,433. DPH is also reporting one additional flu-related death, a 62-year-old female from New Castle County with multiple underlying health conditions, bringing the number of flu-related deaths for the 2017-2018 flu season to 31.

NEW CASTLE (July 26, 2018) – As part of the State Innovation Model (SIM) initiative, the Delaware Health Care Commission is seeking applications from Delaware health care providers for one-time, value-based payment reform mini-grants to grow their capacity to integrate data, improve the coordination of patient care or increase their readiness to integrate into a […]

DOVER – The Delaware Division of Public Health (DPH) continues to advise Delawareans of a multistate outbreak of Salmonella Reading infections linked to raw turkey products. As originally stated Nov. 9, 2018, 164 people infected with the outbreak strain of Salmonella Reading have been reported in 35 states, including one person in Delaware. Of the […]

DOVER  — The number of laboratory-confirmed flu cases continues to increase in Delaware, and the Division of Public Health (DPH) is announcing three recent flu-related deaths, bringing the total number of deaths for the 2018-2019 season thus far to eight. The most recent victim is an 83-year-old man from New Castle County, with underlying health […]

Although it is late in the 2018-2019 flu season, and total weekly influenza cases have begun to decrease, flu activity in Delaware and across the U.S. remains elevated with an increase in the number of influenza A (H3N2) viruses circulating over the last month.

DOVER – Cancer screening and early detection efforts continue to drive down Delaware’s cancer death rates, say state public health officials. In particular, officials are seeing dramatic improvements in the all-site mortality rate among non-Hispanic African-American men, which decreased by 30 percent between the five-year periods of 2001-2005 and 2011-2015, according to the latest cancer […]

The Delaware Division of Public Health (DPH) is advising Delaware residents that it is participating in a multi-state investigation into an outbreak of severe pulmonary disease reported across the country. As of today, 33 states, including Delaware, have reported possible cases of lung illnesses associated with use of e-cigarette products (e.g., devices, liquids, refill pods, and cartridges). While there are no confirmed cases in Delaware at this time, DPH is currently investigating three possible cases among Delaware residents.

The Delaware Division of Public Health (DPH) continues to participate in a multi-state investigation into an outbreak of severe pulmonary disease reported across the country. As of today, 38 states, including Delaware, have reported cases of lung injury associated with use of e-cigarette products (e.g., devices, liquids, refill pods, and cartridges).

Currently in Delaware, there are 11 cases of vaping-related lung injury that meet the Centers for Disease Control and Prevention (CDC) case definition as either probable or confirmed. Additionally, DPH announced today that one of the cases associated with this outbreak involves an individual who died.

As part of National Alzheimer’s Awareness Month, the Delaware Division of Public Health (DPH) urges Delawareans to make lifestyle adjustments to help reduce their risk factors for Alzheimer’s and dementia.

The Delaware Division of Public Health (DPH) announced today that it has confirmed 26 cases of Salmonella in school-aged children in New Castle County that are associated with a multistate outbreak of Salmonella Javiana infections linked to recalled cut fruit.

DOVER – The Division of Public Health (DPH) is reminding Delawareans to protect themselves and those around them this influenza season by getting vaccinated as DPH announces the first two flu-related deaths of the 2019-2020 flu season. A 68-year-old woman infected with influenza A, and a 65-year-old man infected with influenza B, passed away this […]

DOVER – As the number of confirmed influenza cases in Delaware continues to rise, the Division of Public Health (DPH) is announcing two recent flu-related deaths, bringing the total number of deaths for the 2019-2020 season thus far to four. The most recent victim is a 29-year-old New Castle County man diagnosed with influenza B […]

COVID-19 is truly a global health issue affecting everyone and causing concern for you, our customers. As we all continue to navigate the uncertainty of this outbreak, SAS is committed to supporting your business as a valued partner and will ensure continuity of service. We created this guide to address [...]

Frequently asked questions related to COVID-19 and SAS customer support was published on SAS Users.

As of February 15, 2020, there have been 5,047 confirmed cases of influenza in Delaware, including 267 hospitalizations. These numbers reflect only the number of lab-confirmed cases; the actual number of cases circulating statewide is likely much higher. By comparison, at the same time in the 2018-2019 season, there were 3,264 flu cases in Delaware, including 537 hospitalizations, and 13 flu-related deaths.

SMYRNA (March 26, 2020) – The Delaware Division of Public Health (DPH) is confirming the first coronavirus disease 2019 (COVID-19) related fatality in the State of Delaware. The deceased is a 66-year-old male from Sussex County who passed away while hospitalized out of state. The individual had underlying health conditions and was critically ill. The […]