Labour Court had directed firm to pay all allowances as he ‘was not given opportunity to defend himself’

Methods are presented that detect three types of aberrations in single-particle cryo-EM data sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy. Because these methods only depend on the availability of a preliminary 3D reconstruction from the data, they can be used to correct for these aberrations for any given cryo-EM data set, a posteriori. Using five publicly available data sets, it is shown that considering these aberrations improves the resolution of the 3D reconstruction when these effects are present. The methods are implemented in version 3.1 of the open-source software package RELION.

Recent developments have resulted in electron cryo-microscopy (cryo-EM) becoming a useful tool for the structure determination of biological macromolecules. For samples containing inherent flexibility, heterogeneity or preferred orientation, the collection of extensive cryo-EM data using several conditions and microscopes is often required. In such a scenario, merging cryo-EM data sets is advantageous because it allows improved three-dimensional reconstructions to be obtained. Since data sets are not always collected with the same pixel size, merging data can be challenging. Here, two methods to combine cryo-EM data are described. Both involve the calculation of a rescaling factor from independent data sets. The effects of errors in the scaling factor on the results of data merging are also estimated. The methods described here provide a guideline for cryo-EM users who wish to combine data sets from the same type of microscope and detector.

DatView is a new graphical user interface (GUI) for plotting parameters to explore correlations, identify outliers and export subsets of data. It was designed to simplify and expedite analysis of very large unmerged serial femtosecond crystallography (SFX) data sets composed of indexing results from hundreds of thousands of microcrystal diffraction patterns. However, DatView works with any tabulated data, offering its functionality to many applications outside serial crystallography. In DatView's user-friendly GUI, selections are drawn onto plots and synchronized across all other plots, so correlations between multiple parameters in large multi-parameter data sets can be rapidly identified. It also includes an item viewer for displaying images in the current selection alongside the associated metadata. For serial crystallography data processed by indexamajig from CrystFEL [White, Kirian, Martin, Aquila, Nass, Barty & Chapman (2012). J. Appl. Cryst. 45, 335–341], DatView generates a table of parameters and metadata from stream files and, optionally, the associated HDF5 files. By combining the functionality of several commonly needed tools for SFX in a single GUI that operates on tabulated data, the time needed to load and calculate statistics from large data sets is reduced. This paper describes how DatView facilitates (i) efficient feedback during data collection by examining trends in time, sample position or any parameter, (ii) determination of optimal indexing and integration parameters via the comparison mode, (iii) identification of systematic errors in unmerged SFX data sets, and (iv) sorting and highly flexible data filtering (plot selections, Boolean filters and more), including direct export of subset CrystFEL stream files for further processing.

Last week, the Kepler team and CfA astronomers announced the discovery of a system of six transiting planets around one Sun-like star. The previous record holder for the number of transiting planets was three.

The post Six orbiting planets sets record for Sun-like stars say Kepler, Smithsonian astronmers appeared first on Smithsonian Insider.

Marmosets on track for obesity appeared to be more efficient in their feeding behavior. “Although all animals consumed the same amount of liquid, the ones […]

The post Obese marmosets are more developmentally advanced as infants, study shows appeared first on Smithsonian Insider.

(The Paypers) Financial institutions (FIs) working in digital assets have been required by US regulatory bodies to pay attention to their anti-money laundering and...

(The Paypers) The European Banking...

The US Federal Reserve has created a new group to advise on its planned faster payments service, the FedNow...

The vast majority of healthy people adequately meet their daily hydration needs by letting thirst be their guide, says the newest report on nutrient recommendations from the Institute of Medicine of the National Academies.

Most Americans and Canadians up to age 70 need no more than 600 international units (IUs) of vitamin D per day to maintain health, and those 71 and older may need as much as 800 IUs, says a new report from the Institute of Medicine.

The status and potential implications of this deal involving BP as the seller are discussed in a Raymond James report.

A global cropland percentage map and a global field size map have been created for the first time to guide scientists and policymakers interested in global agricultural modelling and assessment. Both maps are for the baseline year 2005 and combined multiple data sets from global, regional and national levels to achieve a high level of accuracy and 1 km2 resolution.

Around 50% of gold in used mobile phones is not recovered for future use, a new study finds. The researchers suggest that a global circular economy in mobile phones could be created by improving recycling of precious metals in phones in developing countries, as well as increasing the lifespan of phones and improving collection after use. These changes will reduce pressures on non-renewable resources and close ‘metal flow loops’.

A new study highlights the economic importance of coastal and marine areas and the urgent need to develop concrete methods for assessing their value. Researchers say the need is especially strong now as climate change's impacts could reduce the economic value of coastal and marine environments.

Countries that appear to have reduced greenhouse gas (GHG) emissions through reforestation may have simply “displaced” the emissions to another country, by increasing their imports of food, timber and wood. A new EU study highlights the need to recognise this ‘loophole’ in ongoing emission targets.

Modular data centres are attractive options to power next-gen applications such as Internet of Things (IoT), industrial process control and smart cities. Radical edge computing possibilities are on offer.

The new center takes the total number of Customer Support Centres of Excellence to ten, five of which are based in Asia.

Shell has agreed to sell its Appalachia shale gas position to National Fuel Gas Co. for $541 million.

The ESA hopes to fly a spacecraft close to Phobos, orbit the lower Venusian atmosphere and draw up a map of Mars' hydrous areas in 2013.

The Maxximus LNG 2000 can do zero to 60 in less than 2 seconds, and can run on propane, CNG, LNG or even gasoline. It's a showcase for the vast potential of nat

As construction begins on a 32-story prefab tower here in the U.S., Broad Sustainable Building Corp. is preparing for a similar project in China that will resul

AT&T customers recycled 50,942 devices in a single week, setting a new Guinness World Record.

A new world record is making the future of solar energy look pretty bright.

Teen sets ambitious goal to be the first astronaut on Mars, and she's harnessing the power of social media to make it happen.

Beyoncé ended the year on a sour note with members of the NASA community.

With so much activism and celebrating going down on Earth Day, a sense of humor is often overlooked. The Onion is here to help.

Travel company cancels offset program citing its inability to reduce global emissions.

MNN chats with guitarist Stone Gossard about the band's latest project and why the group is passionate about making a difference for the environment.

Matt Hickman unravels the mysteries behind offsetting CO2 and buying RECs. (Hint: One of them can fund tree-planting efforts.)

Cheesy trend saw a 450% growth in popularity this year.

Examining layers of offshore sediment can provide insight into the link between glaciers and climate change.

Scientists tracking 278-square-mile block of ice hope to learn from it as it moves away from its parent glacier.

Ford Motor Company plans to use 30% less water per vehicle by 2015.

Video: Adventurer and conservationist David de Rothschild sets sail on Plastiki, a boat made out of 12,000 plastic bottles to help raise awareness about the Gre

Apple juice will now have the same standards as water when it comes to the amount of arsenic allowed, but how much praise does the FDA deserve?

People with celiac disease can now trust that foods labeled "gluten-free" are safe for them to eat.

Dell's 'Plant a Tree for Me' Program Offsets Your Carbon Footprint

Finding bridal jewelry to complete your look for the big day can sometimes be a difficult task. You may find that in your searching you see a pair of earrings you like and a necklace, but there is no bracelet. Likewise, you may also find your bridal earrings and bridal necklace but together, the two pieces of jewelry do not match well. This is where the suggestion of pulling your jewelry accessory look together by purchasing an entire bridal jewelry set from one merchant.

MeetNativeAmericans.com is a perfectly secure dating website that provides all singles with a unique chance to chat and bond with Native Americans.

Get Your Yoga Groove On in 24 Cities Around the Globe

ReductionTech of Kamloops has already proven the technology on 2 other gases and uses ordinary air to make hydroxyl radicals, which are normally mother nature's silver bullet for pollution but they are overwhelmed in urban centers everywhere now.