El Dr. Andrés Vecino, profesor e investigador de la Facultad de Salud Pública de la Universidad Johns Hopkins en EEUU, advierte sobre el posible incremento de la mortalidad durante una intervención a EPS, así como los traumatismos que esta puede generar.

Una investigación de la Contraloría se centra en el manejo de los recursos provenientes de las Unidades de Pago por Capitación. Su carácter es reservado.

En 6AM Hoy por Hoy de Caracol Radio estuvo Germán Córdoba, director del partido político Cambio Radical, para hablar sobre la acción del Gobierno de intervenir a la EPS Sánitas.

Un gran debate generaron la comparación que el ministro Guillermo Alfonso Jaramillo hizo sobre las prepagos y las EPS 

En Caracol Radio estuvo Juan Carlos Giraldo, director de la Asociación Colombiana de Hospitales y Clínicas, conversando sobre las deudas de las IPS

En el recurso judicial, Enrique Vargas mencionó las cuatro publicaciones en las que el presidente Gustavo Petro emitió fuertes críticas sobre los manejos financieros de la Nueva Eps.

It’s the premiere of The Debaters’ 19th season and this is one for the bever-ages! Dave Hemstad and Lisa Baker are in Newfoundland trying to burst each other’s bubble when they decide if Coke is superior to Pepsi. Then, are family doctors overrated? Clifton Cremo and Martha Chaves checkup on these medical professionals.

On this week’s show: Intergovernmental Affairs Minister Dominic LeBlanc talks federal funding to get kids back to school safely. Former leadership hopeful Leslyn Lewis discusses the future of the Conservative Party and her role in it, and two Canadians weigh in on where the party goes from here. Then, retiring Senator Lillian Dyck discusses her legacy and The House looks back at a week of continuing unrest over police brutality in the United States.

A GROUP of teens braved the dark and cold on Friday for a 24-hour challenge as part of Panthers on the Prowl’s pilot program, Building Young Men.

We're giving out cash money!

Plus, Lee debates donating his sperm.

Usher is the ultimate performer and musical inspiration.

Arsenio Hall is the host for the 2016 BET Honors show.

Zia Chishti — an American businessman who served as the CEO of Bermuda-based Afiniti — has stepped down after a former employee alleged that he sexually assaulted her. The company said, “The Board of Directors of Afiniti, Ltd. announces that Mr. Zia Chishti has stepped down from his role as Chairman, Chief Executive Officer, and […]

[Written by Freddie Forth & Martha Harris Myron] Introducing Freddie Forth, owner of Forthright Solutions. Freddie is a Bermudian luminary expert in real estate, mortgages & lending, local architecture and author of Eight Steps to Home Ownership Freddie Forth has volunteered her time and expertise in creating the Eight Steps of Home Ownership in short, […]

“The Steps to a Well Bermuda survey is crucial for us to better understand and improve the health of our community, and we need those who have been selected to participate to please do so,” said Minister of Health, Kim Wilson. “In partnership with the Pan American Health Organization, this survey will give us a […]

REVIEW – When we lived in Europe, our home had a heated towel bar in the bathroom. It seemed like such a decadent little thing, but as fall turns to winter I recall just how nice it was to step out of the shower and reach for a warm towel. When the Comfier towel and […]

We've all been there: you're about to heat up your favorite meal, and you notice your microwave is splattered with remnants of meals past. It's not just unsightly; it can also affect the taste and smell of your food. That's why I'm here to share my go-to tips for getting your microwave sparkling clean without a fuss.

The post Quick Microwave Cleaning: Safe & Easy Steps appeared first on Unclutterer.

Good morning! Today, we’re going to start the finishing process for Harvest Hues – yippee!! Due to the length of …

Yesterday, we began the finishing process on Harvest Hues, and today, we are going to tie up the project! In …

Resilience is the key to transforming challenges into opportunities for personal growth. It’s not just about bouncing back from setbacks—it’s about thriving despite them. Resilience is not something you either have or you don’t. Instead, it is a skill you can cultivate and strengthen over time. In this article, we’ll explore the multifaceted nature of ... Read more

The post How to Develop Resilience: Key Steps for Thriving Through Adversity appeared first on LifeHack.

Ain't no feed grain too good for mah peeps.

Show Notes for Episode 4: Subscribe to the Internet Musician Podcast with iTunes: As a musician, you need a good website.  You know that. But you really don’t need to spend years trying to understand the technology. You also don’t want to spend tons of time working on your site without knowing if you’re doing things […]

Budget season is upon us, and everyone in your organization is vying for their slice of the pie. Every year, every department has a pet project that they present as absolutely essential to profitability, business continuity, and quite possibly the future of humanity itself. And no doubt that some of these actually may be mission critical. But as cybersecurity professionals, we understand that

The underlying cause of multiple sclerosis is not yet known, but Epstein-Barr virus is a possible culprit, Harvard researchers say.

It’s refreshing to see manufacturers still making sedans that the average South African family can afford. The Volkswagen Polo Sedan is one of those cars, but now it’s bigger than the previous generation and a lot more modern.

The rental-car giant fell as much as 12% on Tuesday after posting a deeper-than-expected loss.

Finding ways to save money is much easier than you think, because you’re probably spending money you don’t have to spend.

The article 3 Easy Steps to Save Money for Travel originated at EverInTransit.com

Next steps for EU-US cooperation on trade and technology 8 December 2022 — 3:00PM TO 4:00PM Anonymous (not verified) 21 November 2022 Online

How can the EU and US increase cooperation on AI, semi-conductors and funding information communication technology services?

On trade and technology policy, the EU and the US are making meaningful progress towards cooperation while at the same time navigating tensions. As senior officials meet on 5 December for the third meeting of the Trade and Technology Council (TTC), both sides have vowed to move towards concrete results. But can the US and EU increase cooperation on artificial intelligence, semiconductors, and funding information communication technology services? 

This event draws on insights from a forthcoming Briefing Paper by Marianne Schneider-Petsinger that explores next steps for US-EU cooperation on trade and technology, which is part of a project supported by the Hanns Seidel Foundation. 

Mohamad Navab
Sep 1, 2000; 41:1495-1508
Articles

Mohamad Navab
Sep 1, 2000; 41:1481-1494
Articles

Phospholipase Cε (PLCε) is activated downstream of G protein–coupled receptors and receptor tyrosine kinases through direct interactions with small GTPases, including Rap1A and Ras. Although Ras has been reported to allosterically activate the lipase, it is not known whether Rap1A has the same ability or what its molecular mechanism might be. Rap1A activates PLCε in response to the stimulation of β-adrenergic receptors, translocating the complex to the perinuclear membrane. Because the C-terminal Ras association (RA2) domain of PLCε was proposed to the primary binding site for Rap1A, we first confirmed using purified proteins that the RA2 domain is indeed essential for activation by Rap1A. However, we also showed that the PLCε pleckstrin homology (PH) domain and first two EF hands (EF1/2) are required for Rap1A activation and identified hydrophobic residues on the surface of the RA2 domain that are also necessary. Small-angle X-ray scattering showed that Rap1A binding induces and stabilizes discrete conformational states in PLCε variants that can be activated by the GTPase. These data, together with the recent structure of a catalytically active fragment of PLCε, provide the first evidence that Rap1A, and by extension Ras, allosterically activate the lipase by promoting and stabilizing interactions between the RA2 domain and the PLCε core.

Yi-Han Lin
Dec 1, 2020; 19:2030-2046
Research

Aloïs Dusuel
Dec 9, 2020; 0:jlr.RA120000704v1-jlr.RA120000704
Research Articles

Bacterial lipopolysaccharides (LPSs or endotoxins) can bind most proteins of the lipid transfer/LPS-binding protein (LT/LBP) family in host organisms. The LPS-bound LT/LBP proteins then trigger either an LPS-induced proinflammatory cascade or LPS binding to lipoproteins that are involved in endotoxin inactivation and detoxification. Cholesteryl ester transfer protein (CETP) is an LT/LBP member, but its impact on LPS metabolism and sepsis outcome is unclear. Here, we performed fluorescent LPS transfer assays to assess the ability of CETP to bind and transfer LPS. The effects of intravenous (iv) infusion of purified LPS or polymicrobial infection (cecal ligation and puncture [CLP]) were compared in transgenic mice expressing human CETP and wild-type mice naturally having no CETP activity. CETP displayed no LPS transfer activity in vitro, but it tended to reduce biliary excretion of LPS in vivo. The CETP expression in mice was associated with significantly lower basal plasma lipid levels and with higher mortality rates in both models of endotoxemia and sepsis. Furthermore, CETPTg plasma modified cytokine production of macrophages in vitro. In conclusion, despite having no direct LPS binding and transfer property, human CETP worsens sepsis outcomes in mice by altering the protective effects of plasma lipoproteins against endotoxemia, inflammation, and infection.

Sepsis-induced acute kidney injury (S-AKI) is the most common complication in hospitalized and critically ill patients, highlighted by a rapid decline of kidney function occurring a few hours or days after sepsis onset. Systemic inflammation elicited by microbial infections is believed to lead to kidney damage under immunocompromised conditions. However, although AKI has been recognized as a disease with long-term sequelae, partly because of the associated higher risk of chronic kidney disease (CKD), the understanding of kidney pathophysiology at the molecular level and the global view of dynamic regulations in situ after S-AKI, including the transition to CKD, remains limited. Existing studies of S-AKI mainly focus on deriving sepsis biomarkers from body fluids. In the present study, we constructed a mid-severity septic murine model using cecal ligation and puncture (CLP), and examined the temporal changes to the kidney proteome and phosphoproteome at day 2 and day 7 after CLP surgery, corresponding to S-AKI and the transition to CKD, respectively, by employing an ultrafast and economical filter-based sample processing method combined with the label-free quantitation approach. Collectively, we identified 2,119 proteins and 2950 phosphosites through multi-proteomics analyses. Among them, we identified an array of highly promising candidate marker proteins indicative of disease onset and progression accompanied by immunoblot validations, and further denoted the pathways that are specifically responsive to S-AKI and its transition to CKD, which include regulation of cell metabolism regulation, oxidative stress, and energy consumption in the diseased kidneys. Our data can serve as an enriched resource for the identification of mechanisms and biomarkers for sepsis-induced kidney diseases.

In eukaryotic DNA replication, DNA polymerase ε (Polε) is responsible for leading strand synthesis, whereas DNA polymerases α and δ synthesize the lagging strand. The human Polε (hPolε) holoenzyme is comprised of the catalytic p261 subunit and the noncatalytic p59, p17, and p12 small subunits. So far, the contribution of the noncatalytic subunits to hPolε function is not well understood. Using pre-steady-state kinetic methods, we established a minimal kinetic mechanism for DNA polymerization and editing catalyzed by the hPolε holoenzyme. Compared with the 140-kDa N-terminal catalytic fragment of p261 (p261N), which we kinetically characterized in our earlier studies, the presence of the p261 C-terminal domain (p261C) and the three small subunits increased the DNA binding affinity and the base substitution fidelity. Although the small subunits enhanced correct nucleotide incorporation efficiency, there was a wide range of rate constants when incorporating a correct nucleotide over a single-base mismatch. Surprisingly, the 3'→5' exonuclease activity of the hPolε holoenzyme was significantly slower than that of p261N when editing both matched and mismatched DNA substrates. This suggests that the presence of p261C and the three small subunits regulates the 3'→5' exonuclease activity of the hPolε holoenzyme. Together, the 3'→5' exonuclease activity and the variable mismatch extension activity modulate the overall fidelity of the hPolε holoenzyme by up to 3 orders of magnitude. Thus, the presence of p261C and the three noncatalytic subunits optimizes the dual enzymatic activities of the catalytic p261 subunit and makes the hPolε holoenzyme an efficient and faithful replicative DNA polymerase.

Struggling to be kind to yourself? Learn how to direct compassion inwards with these simple techniques.

Are you the kind of person who needs to know what keeps satellites from plummeting to the Earth in a big, fiery ball? Then you need to watch this one-minute video, where Ask Smithsonian host Eric Schulze gives us the lowdown on what-in-the-name-of-science makes those satellites stay up.

Read more at http://blogs.smithsonianmag.com/science/2010/04/01/a-peep-experiment/ Our Surprising Science blogger tests whether stale peeps or fresh peeps are better for the spring tradition of peep jousting.

Julie Montagu, the American-born Viscountess Hinchingbrooke, is meeting up with an expert on 18th century dance. Her aim is to learn about the dances performed in that era.

Team Reid Carruthers has started the search for a new second after Derek Samagalski's decision to step back from the Winnipeg-based rink.

Every November, Jan Diehl adorns her Martensville, Sask., home with a special Remembrance Day tribute.

Not only does Mary the Asian elephant prefer to shower herself, but she's really good at it. So good, in fact, that her dexterous bath-time ritual is the subject of a new study about animal tool use. 

Anna (Germany) and Christina (Canada) are the youngest members of OM Montenegro. They describe how God called them into missions, and led them unexpectedly to Montenegro, a Balkan country neither had heard much about before. Here God is changing them as they prepare for further service...

Partnership with 3DVision Technologies Reduces Materials and Manufacturing Costs

Name: Sam Castro Home: Pachuca, Mexico Born in: March 1988 Joined OM Ships: September 2013 Previous employment: Veterinarian Current job on board: Shift leader in the book fair