The invention provides compositions and methods for treating leukemia, for example, acute myeloid leukemia (AML) and B-cell acute lymphoid leukemia (B-ALL). The invention also relates to at least one chimeric antigen receptor (CAR) specific to CD123, vectors comprising the same, and recombinant T cells comprising the CD123 CAR. The invention also includes methods of administering a genetically modified T cell expressing a CAR that comprises a CD123 binding domain. The invention also includes methods of bone marrow ablation for use in treatments necessitating bone marrow reconditioning or transplant.

A recombinant vector comprises simian adenovirus 36, simian adenovirus 42.1, simian adenovirus 42.2 and/or simian adenovirus 44 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses one or more simian adenovirus-36, -42.1, -42.2 or -44 gene(s) is also described. Methods of using the vectors and cell lines are provided.

The present invention relates to the use of the miRNA expression profile, particularly of miR-199a-5p, and the target genes regulated thereby for the diagnosis, prognosis and use of miR-199a-5p inhibitors for treating fibroproliferative disorders.

Provided herein are compositions and methods for modifying a predetermined nucleic acid sequence. A programmable nucleoprotein molecular complex containing a polypeptide moiety and a specificity conferring nucleic acid (SCNA) which assembles in-vivo, in a target cell, and is capable of interacting with the predetermined target nucleic acid sequence is provided. The programmable nucleoprotein molecular complex is capable of specifically modifying and/or editing a target site within the target nucleic acid sequence and/or modifying the function of the target nucleic acid sequence.

This disclosure relates to methods, computer products, computer-implemented methods, and systems for predicting the probability or risk of prostate cancer recurrence and tumor aggressiveness in a patient. The method is based, in part, on the patient's risk profile that includes the presence/degree of specific copy number variations and predictive clinical factors. The methods and systems can be used to aid in treatment selection.

The invention provides for a proteomic approach for identification of specific bacterial protein profiles that may be used in the development of methods for the diagnosis of bacterial chronic sinusitis. The invention provides for methods for determining the presence of pathogenic bacteria in the upper respiratory tract of a subject using protein profiles of the pathogenic bacteria. The invention also provides for methods of diagnosing a bacterial infection of the upper respiratory tract of a subject using protein profiles of a pathogenic bacteria. In addition, the invention provides for devices, immunoassays and kits for identifying pathogenic bacteria in the upper respiratory tract.

A button and pin assembly machine for producing badges and the like comprising buttons having rearwardly extending rims adapted to receive and retain respective pin structures therein, in which the buttons are sequentially supplied to a pin-forming head and supported thereat with the button rim encircling the end of such head, straight pin blanks being sequentially supplied to said head, with a portion of a pin blank being inserted into the head and rotated thereby with respect to a stationary abutment to curl an end of such blank sufficiently to enable its insertion in the rim of a button disposed thereat, said head having means for forming a bend in the portion of the pin blank inserted in such head to resiliently bias the pointed end of the pin blank toward the button.

Device for forming buttons from a pin-on back plate, a front plate, and a cover over the front plate, with the device having a pair of button-forming dies alternately shiftable into operation alignment with a forming punch which on descent with the successively aligned dies cooperates with the latter to assemble the separate button parts into a button, and a cover-blanking punch and die set above the forming punch, of which the blanking die is topmost and has a die aperture open at the top and bottom and a cutting edge at the bottom of the aperture, with the blanking die being by the forming punch raised into blanking cooperation with the blanking die, and the forming punch being moved upwardly and downwardly by manually operable means.

Device for forming buttons from a pin-on back plate, a front plate, and a cover over the front plate, with the device having a pair of button-forming dies alternately shiftable into operative alignment with a forming punch which on descent into the successively aligned dies cooperates with the latter to assemble the separate button parts into a button, and a blanking punch and die set above the forming punch, of which the blanking punch is raised into cooperation with the blanking die for blanking a cover from a supply sheet, and a handlever operatively connected with the punches for simultaneously lowering the forming punch and raising the blanking punch.

A method and apparatus for substantially automatically joining front and back parts of buttons employs a turntable with retaining means that are rotated through a series of stations at which the parts are placed in the retaining means in a desired orientation, joined, and checked for defects. A human operator places button front parts at a first station, and the remaining stations carry out the assembly and quality control steps automatically. Placement at the first station determines the alignment and orientation of the two buttons.

A method for forming a multi-colored cap part of a snap button including the steps of forming a design portion of the cap part of snap button using a first group of molds, which includes a first female mold, a first male mold, and a plate-shaped middle mold, and a slide-plate mold adapted to movably assembled to the first male mold and having a plurality of mold cavities for forming the design portion; removing the slide-plate mold having the design portion molded therein from the first male mold; movably positioning the slide-plate mold into a second female mold included in a second group of molds for forming a body portion of the cap part of snap button; positioning a post for the cap part of snap button in a second male mold of the second group of molds; and forming the body portion.

A soy protein product having a protein content of at least 60 wt % (N×6.25) d.b., preferably an isolate having a protein content of at least about 90 wt % (N×6.25) d.b., is formed from the supernatant from the precipitation of a soy protein micellar mass. A calcium salt or other divalent salt is added to the supernatant, before concentration, after initial concentration or after final concentration, to provide a conductivity of about 2 to about 30 mS. Precipitate is removed from the resulting solution and the pH of the clear soy protein solution is optionally adjusted to about 1.5 to about 4.4. The optionally pH-adjusted clear solution is concentrated to a concentration of about 50 to about 400 g/L and the clear concentrated protein solution is optionally diafiltered prior to drying. The soy protein product is soluble in acidic media and produces transparent, heat stable solutions at low pH values and, therefore, may be used for protein fortification of soft drinks and sports drinks.

A method for removing 4-methylimidazole (4-MEI) from solution may include contacting an alkaline earth metal silicate with a solution containing 4-MEI and adsorbing at least some of the 4-MEI using the alkaline earth metal silicate. The method may further include removing at least some of the alkaline earth metal silicate having the adsorbed 4-MEI from the solution. The alkaline earth metal silicate may include magnesium silicate or calcium silicate. A method for removing 4-MEI from solution may include contacting an adsorbent clay material with a solution containing 4-MEI and adsorbing at least some of the 4-MEI using the adsorbent clay material. The method may further include removing at least some of the adsorbent clay material having the adsorbed 4-MEI from the solution. The adsorbent clay material may include smectite, bentonite, or an activated or un-activated AOCS day material.

Pancake mixes comprising at least one type of whole grain flour, at least one natural sweetener, at least one fat and a leavening system are provided herein. Packaged food products comprising single-serving containers containing said pancake mixes, as well as methods of preparing pancakes, are also provided. The pancakes described herein are preferably 100% whole grain and free from preservatives and/or artificial colorings.

This disclosure describes a simple, low cost method to produce a modular dry mix that can easily be modified and customized to produce a wide range of easy to aerate, no ice cream machine needed, ice cream and frozen dairy and non-dairy desserts and products made by this process. The method relies on successive simultaneous particle size reduction and blending steps without the use of traditional mix drying operations. The formulations produced by this process are modular and allow quick and simple ingredient substitutions to create distinct finished products. The process yields mixes that produce stronger, more stable foams that don't leave an oily mouth feel. The disclosure also includes a formulation and process used to make ice cream, and related frozen dairy and non-dairy desserts at room temperature in one step where the ingredients do not need to be aged, or homogenized, and aeration occurs without the need for simultaneous cooling or freezing. The process allows the end-user to control and vary the texture, freezing rate, melting point and eating qualities of the product without the use of traditional, home or industrial ice cream machines and a product made by this process. A product produced from this mix consists of a (protein-concentrate and emulsifier)—stabilized, protein-based foam created by combining a powder mix with one or a combination of a large range of above freezing temp liquids then immediately whisking the mixture at room temp to a target overrun without pre-aging, and then freezing that product statically till desired hardness is achieved. The main execution of this process involves the use of dry powders that are transported and sold dry and then reconstituted by the end user before they are combined without pre-treatment, freezing homogenization or aging. The manufacturing process offers significant advantages over current dry mix production methods which require significant upfront investment and produce hard to customize mixes. The formulation and finished product making portions of the invention offer significant improvement over existing manufacturing methods for frozen desserts and dessert mixes that require simultaneous freezing and aeration in terms of cost, convenience, customizability, shelf-stability and environmental impact.

A beverage dispensing system comprises a container, an attachment mechanism, and a cartridge. The container may hold a mixing solution or liquid, such as water, to be mixed with the contents of the cartridge. The attachment mechanism is generally located within the container. The cartridge generally engages with the attachment mechanism to open the cartridge to be dispensed into the container, thereby combining the contents of the cartridge with the liquid within the container to create a drinkable beverage. The cartridge may include a unique identifier to track a set of beverage characteristics associated with the contents of the cartridge and the beverage. The container may capture the unique identifier of the cartridge associated with the set of beverage characteristics and transfer the set of beverage characteristics over a wireless network to a computing device or network.

A milling tool holder wrench is provided. A wrench includes two knobs that protrude outward and perpendicular from a surface of the wrench at a top end of a u-shaped portion of the wrench. The knobs adapted to snuggly engage recessed fittings of a milling tool holder to lock the milling tool holder steady within a milling machine while a tool is changed from the milling tool holder.

Disclosed is a two-needle sewing machine that improves strength in a convex curve part by bringing a pair of stitches close together when sewing an item. A two-needle sewing machine includes a bed part; a column part; an arm part; a needle shaft that is disposed on the leading part of the arm part; two sewing needles that are attached to the needle shaft; two horizontal hooks that catch the respective needle thread loops of the two sewing needles; and a conveyor member that conveys a material to be sewn in a direction approximately orthogonal to the bed part. On the two-needle sewing machine, the sewing needle, which is positioned in a location distant from the column part, is disposed closer to the conveyance side of the material to be sewn than the sewing needle that is positioned in a location closer to the column part.

A carpet and method for producing same that comprises an enhanced seam suitable for separation of an installation portion of the carpet and sacrificial edge portions of the produced carpet.

Methods for manufacturing laminated membranes for MEAs, such methods comprising (i) providing a substrate, a catalyst ink fluid, and a first membrane fluid; (ii) providing a second membrane fluid; (iii) simultaneously coating the catalyst ink fluid onto the substrate, the first membrane fluid onto the catalyst ink fluid, and the second membrane fluid onto the first membrane fluid; and (iv) applying a reinforcement layer (such as ePTFE) and allowing for full imbibement. The second membrane fluid (i) consists of alcohol or (ii) is an alcohol-rich fluid comprising polymer electrolyte.

Provided is a method of producing an artificial marble containing amethyst and vermiculite. The artificial marble includes amethyst representing superior performance in terms of a far-infrared emission effect and an antimicrobial property with a fine appearance, and vermiculite serving as an inorganic filler material and representing a superior adiabatic effect with a light weight. The artificial marble has a structure in which the first base layer including amethyst and the second base layer including vermiculite are laminated on each other.

A method for fabricating a repair laminate for a composite part having an exposed surface includes applying a bonding material to the exposed surface and forming an uncured ply stack assembly on the bonding material. The uncured ply stack assembly is formed by forming and compacting a series of uncured ply stacks. The ply stack assembly and bonding material are then cured.

A temporary adhesive for reversibly bonding a silicon wafer to a silicon support contains a crosslinkable organopolysiloxanes composition and a fatty acid or salt thereof or fatty acid ester as a release regulator having a density different from the crosslinkable organopolysiloxanes composition of at least 0.1 g/cm3, which upon parting of the wafer from the substrate, the adhesive remains substantially adhered to the substrate.

A thin film forming apparatus and a thin film forming method using the same are disclosed. In one aspect, the thin film forming apparatus comprises a mask that includes a blocking portion and an opening. It also includes an etching source that jets an etching gas through the opening of the mask to etch a thin film according to a pattern. The mask includes a gas blower for blowing a gas around the opening so that the etching gas does not penetrate into a thin film area corresponding to the block portion. When the thin film forming apparatus is used, a normal residual area of a thin film may be safely preserved and patterning may be accurately performed. Thus, the quality of a product manufactured by using the thin film forming apparatus may be improved.

A system for fluid partitioning for chemical amplification or other chemical processing or separations of a sample, comprising a first dispenser of a first polymeric sheet, wherein the first polymeric sheet contains chambers; a second dispenser of a second polymeric sheet wherein the first dispenser and the second dispenser are positioned so that the first polymeric sheet and the second polymeric sheet become parallel; a dispenser of the fluid positioned to dispense the fluid between the first polymeric sheet and the second polymeric sheet; and a seal unit that seals the first polymeric sheet and the second polymeric sheet together thereby sealing the sample between the first polymeric sheet and the second polymeric sheet and partitioning the fluid for chemical amplification or other chemical processing or separations.

A ceramic green sheet laminate is produced by stacking ceramic green sheets, each including conductive films for forming first or second internal electrodes on a surface thereof. A first cutting step is performed in which the ceramic green sheet laminate is cut to form first and second end surfaces at which the first or second internal electrodes are exposed. A second cutting step is performed in which the ceramic green sheet laminate is cut to form first and second side surfaces at which the first and second internal electrodes are exposed. In the second cutting step, the ceramic green sheet laminate is pressed and cut by moving a cutting blade in a length direction or a width direction.

A method for detaching a semiconductor chip from a foil uses a die ejector comprising plates having a straight supporting edge and an L-shaped supporting edge comprises: lifting of the plates to a height H1 above the surface of a cover plate;lowering of a first pair of plates with L-shaped supporting edge;optionally, lowering of a second pair of plates with L-shaped supporting edge;lifting of the plates that have not yet been lowered to a height H2>H1;staggered lowering of plates that have not yet been lowered, with at least one or several plates not being lowered;optionally, lowering of the plates that have not yet been lowered to a height H3

A method and processes to solubilize and transform phosphorus contents of rock phosphate (RP) into bio-organo-phosphate (BOP) fertilizer have been developed and integrated. The methods include collecting and sorting of organic wastes; blending with RP; subjecting the blend to biocomposting; collection, isolation, selection and growth optimization of consortia of efficient phosphorus solubilizing microorganisms (PSM) and novel plant growth regulating microorganisms (PGRM); where in PSM produce organic acids and other organic compounds using compost substrate at mesophillic stage, whereas the organic acids released during composting also act in synergism of PSM, thus forming a carbon rich acidic culture resulting in the solubilization of rock phosphate.

The present disclosure is directed toward a composition and method of treating and preventing infection of pathogenic microorganisms and endopyhtic microorganisms in a plant through the use of phosphite compositions.

The present invention relates to a method for removing sulfur from a gaseous or liquid mixture. This method involves contacting the gaseous or liquid mixture with an iron-enriched matrix under conditions effective to remove sulfur from the mixture through adsorption of the sulfur to the matrix. The iron-enriched matrix used in this method is a lignocellulosic material that is enriched with iron. The present invention also relates to a system, composition, and plant fertilizer that contain the iron-enriched matrix. Methods of making the composition and preparing a plant fertilizer are also disclosed.

An esteramide compound of the following formula (I): R1OOC-A-CONR2R3 (I) is described, wherein: A is a covalent bond or a methylene group —CH2—;R1 is an optionally substituted, saturated or unsaturated aliphatic or cycloaliphatic hydrocarbon group including from 5 to 36 carbon atoms,R2 and R3, either identical or different, are groups selected from a hydrogen atom, and optionally substituted hydrocarbon groups, preferably including from 1 to 36 carbon atoms, andR2 and R3 not being simultaneously hydrogen atoms. Also described, are applications for using the esteramide compound, notably as a solvent.

The invention relates to an aqueous solution of a mixture for treating urea-based fertilizers/fertilizers containing ammonium that contains 1,2,4-triazole and 3-methylpyrazole in the weight ratio 2/1 and at least one polyvinyl alcohol, polyvinylpyrrolidone and a tenside.

A granular agrochemical composition is disclosed including a granular core material having a water soluble portion with a first coating layer applied on the surface of the core material and a second coating layer applied on the surface of the first coating layer. The first coating layer includes a wax composition having a biologically active ingredient incorporated therein and the second coating layer includes a polymeric composition. The granular agrochemical composition exhibits a controlled rate of release of the biologically active ingredient therefrom over a period greater than about 30 days from the date of initial exposure of the granular composition to moisture whereby essentially all of the biologically active ingredient incorporated in the wax material of the first coating layer is released from the granular composition before the water soluble portion of the granular core material is released from the granular composition.

A composition of matter comprising: a combination of a phytate and a plurality of microorganisms comprising a Trichoderma virens fungus, a Bacillus amyloliquefaciens bacterium, and one or a plurality of mycorrhizae fungi that is placed in the vicinity of a plant root in a manner that allows the microorganisms in the composition of matter to colonize said plant root; and a method for increasing plant yield comprising: placing a combination of a phytate and a plurality of microorganisms comprising a Trichoderma virens fungus, a Bacillus amyloliquefaciens bacterium, and one or a plurality of mycorrhizae fungi in the vicinity of a plant root in a manner that allows the microorganisms in the composition of matter to colonize said plant root.

Semi-autonomous vehicle apparatus which is controlled by a plurality of control sources includes a vehicle which may function autonomously and apparatus for control of the vehicle by either an onboard driver or a driver not situated onboard. The vehicle may also be controlled by an off-vehicle computational device. Hierarchy setting apparatus determines which of the possible control entities take priority. Persons using the apparatus are identified by either a password or, preferably by providing identification based on a biologic feature. Management of impaired vehicle operators is provided for.

Techniques for navigating semi-autonomous mobile robots are described. A semi-autonomous mobile robot moves within an environment to complete a task. A navigation server communicates with the robot and provides the robot information. The robot includes a navigation map of the environment, interaction information, and a security level. To complete the task, the robot transmits a route reservation request to the navigation server, the route reservation request including a priority for the task, a timeslot, and a route. The navigation server grants the route reservation if the task priority is higher than the task priorities of conflicting route reservation requests from other robots. As the robot moves within the environment, the robot detects an object and attempts to classify the detected object as belonging to an object category. The robot retrieves an interaction profile for the object, and interacts with the object according to the retrieved interaction profile.

A map screen determining method and apparatus, which includes obtaining profile data in a track dimension of a target digital person, where the target digital person is generated by a digital person generation system and consists of multiple dimensions of target user profiles corresponding to a target user, and the target user profiles are generated by processing multiple dimensions of data from multiple data sources, determining map data of a target area, where the target area is a specific map area that needs to be presented to a user, and determining a tracing map screen according to the profile data in the track dimension of the target digital person and the map data, where the tracing map screen represents a track feature of the target digital person in the target area.

A system for determining hang time for a machine operating at a worksite is provided. The system includes a sensing module associated with the machine. The sensing module is configured to track at least one parameter associated with the machine. The at least one parameter includes a machine heading, a machine speed, or a machine location. The system also includes a control module communicably coupled to the sensing module. The control module is configured to receive tracked information corresponding to the at least one parameter from the sensing module. The control module is also configured to analyze the tracked information corresponding to the at least one parameter, based on the received tracked information. The control module is further configured to determine the hang time for the machine, based on the analysis.

A method for finding an evasive trajectory for avoiding an obstacle for a vehicle on a roadway. A component of a candidate trajectory parallel to the roadway is determined by selecting weighting coefficients of a first weighted sum of orthogonal functions of time. A component of the candidate trajectory orthogonal to the roadway is determined by selecting weighting coefficients of a second weighted sum of the orthogonal functions. An optimization parameter for the candidate trajectory is calculated. At least one coefficient of at least one of the sums is modified and the procedure is repeated when the optimization parameter does not reach a termination criterion.

A method for ascertaining a rotational speed parameter for determining a setpoint torque for driving a drivetrain. The drivetrain comprises a first and at least one second drive assembly for driving a hybrid vehicle. The first drive assembly can be coupled to the drivetrain by means of a clutch. The second drive assembly is mechanically coupled to the drivetrain. When the hybrid vehicle is being driven by means of at least the first drive assembly, the rotational speed parameter corresponds to the value of a shaft rotational speed. When the hybrid vehicle is being driven only by means of the second drive assembly, the rotational speed parameter corresponds to the value of a determined rotational speed.

A patient side-system includes a column with a rail and a counterbalance subsystem. The patient side-system may further include a braking subsystem. The counterbalance subsystem includes a spring assembly coupled at one end to the column with a spring member, and a housing movably coupled to the rail. The housing includes a drum to receive the spring member and a plurality of roller elements to guide a movement of the spring member winding or unwinding on the drum. If present, the braking subsystem includes a first pulley rotatably coupled to the column, a second pulley with a locking mechanism spaced apart from the first pulley and rotatably coupled to the column, and at least one brake cable wrapped around the first pulley and the second pulley with ends coupled to the housing. The locking mechanism can set a position of the housing along the column.

An image forming apparatus includes a controller configured to control a first image former to form a first test pattern on a recording medium, the first test pattern including figures arranged in a first direction on a two-dimensional lattice defined with an X-axis direction intersecting a Y-axis direction parallel to a conveyance direction, the first direction being inclined relative to the X-axis direction, control a conveyor to convey the recording medium in the conveyance direction, and in response to the recording medium being conveyed to a position where a second test pattern intersects or is in proximity to the first test pattern, control a second image former to form the second test pattern on the recording medium, the second test pattern including figures arranged in a second direction on the two-dimensional lattice, the second direction being inclined relative to each of the X-axis direction and the first direction.

The instant disclosure relates to a customizable vacuum molding method for forming personalized luggage bags utilizing a customizable vacuum molding device having a main mold body (1) and a personalized pattern module (2) customized to suit a customer preference. The vacuum molding method comprising the steps of: 1) placing the personalized pattern module on a molding surface of the main mold body at a predetermined location; 2) placing a plastic luggage bag substrate onto the molding surface of the vacuum molding device and soften the substrate by heating; and 3) generating negative pressure in the vacuum molding device and stretching the plastic substrate onto the personalized pattern module on the molding surface of the main mold body while cooling the plastic substrate to form a personalized pattern. The instantly disclosed vacuuforming method provides a simple yet cost effective solution for manufacturing luggage bags having personalized patterns of a customer's choice.

The present invention is a smart luggage having an outer case and an inner case with a weight determination mechanism therebetween in electrical communication with a circuit having firmware and an exterior electronic display. The circuit further incorporates a GPS locator and a transponder, whereby a user having a mobile device enabled with tracking software may be able to locate and retrieve lost or stolen luggage. Also provided within the smart luggage is a camera that can take one or more pictures when the smart luggage is opened by a person.

A micromechanical component is described having a substrate which has at least one stator electrode fixedly mounted with respect to the substrate, a movable mass having at least one actuator electrode fixedly mounted with respect to the movable mass, and at least one spring via which the movable mass is displaceable. The movable mass is structured from the substrate with the aid of at least one separating trench, at least one outer stator electrode spans at least one section of the at least one separating trench and/or of the movable mass, the at least one actuator electrode protrudes between the at least one outer stator electrode and the substrate, and at least one inner stator electrode protrudes between the at least one actuator electrode and the substrate. A related manufacturing method is also described for a micromechanical component.

An apparatus and a method for determining a damage state of a wheel bearing (12), in which a wheel (15) with a tire (9) arranged on a rim (8) in such a manner that the wheel can be rotated on an axle, in a motor vehicle, in particular during maintenance work on the motor vehicle. In order to avoid the wheel bearing (12) being accessed unnecessarily, a sensor unit (1) which communicates with an evaluation unit and is removably fastened radially outside a screw circle (11) of the rim (8) is provided for the purpose of determining the damage state.

A pressure transmitter has a pressure sensor, an isolator diaphragm, and a fill tube. Interior passages in the pressure sensor module body are filled with isolator fluid and provide fluid connections. The isolator fluid couples pressure from the first isolator diaphragm assembly to the pressure sensor. A first crimp portion of the fill tube radially narrows into a substantially solid circular cylindrical cross section to form a first primary seal that is resistant to high pressure cycling.

The invention relates to a method for determining the fatigue capital of a cable supporting a civil engineering structure, the method including: a measuring step (S1, S2) during which the normal tensile stress in the cable and the bending stress in the cable are measured in a synchronized manner so as to obtain the compound stress in the cable; a counting step (S3) during which a count of the number of stress cycles, depending on the amplitude of the stress, is carried out from the measured compound stresses; and a step (S4) of assessing the fatigue capital of the cable during which the fatigue capital of the cable is determined by comparing the count, carried out in the counting step, with a pre-set Wohler curve for the cable.

A control system according to the principles of the present disclosure includes a fuel system diagnostic module and a pump control module. The fuel system diagnostic module performs a diagnostic test on a fuel system when a vacuum pump is switched off to prevent flow through the vacuum pump and thereby seal a portion of the fuel system from an atmosphere. The vacuum pump includes a cam ring, a rotor that rotates within the cam ring, and vanes that slide into and out of slots in the rotor as the rotor rotates. The pump control module switches on the vacuum pump for a predetermined period to force the vanes out of the slots and into contact with the cam ring to seal the vacuum pump before switching off the vacuum pump for the fuel system diagnostic test.