Making the most of your available space.

New year, new career?

Panelistas consideran que la medida busca aliviar las finanzas de un sector golpeado, pero creen que puede tener un efecto sobre otros sectores que busquen el mismo beneficio.

http://www.musicxray.com/xrays/2672490 KadoMane - Im Taxin'em

James sought happiness through the world’s pleasures but was left feeling empty. When he found Bible Answers Live on the radio, he began listening often. The Holy Spirit convinced him to get serious about what he heard, and his life turned in a much better direction. You can guide even more people like James to find heaven’s answers to the deepest needs of their hearts when you support Amazing Facts radio. Your gifts open galaxies of truth to people wanting more than what this world offers. Thank you for giving!

La Luciérnaga se enciende para hablar del posible paro de taxistas. Además, le contamos cómo se prepara Bogotá para recibir el puente de reyes. Asimismo, el curioso cartel publicado por la policía de Santa Marta que se convirtió en un meme.

La Luciérnaga se enciende para hablar de cómo se desarrollaron las movilizaciones de taxistas a lo largo del país. Además, le contamos sobre lo qué pasa con una moción de censura contra la ministra de Minas. También, ¿El gabinete del presidente Petro está a punto de quedarse con vacantes?La Luciérnaga, un espacio de humor, análisis y opinión de Caracol Radio que acompaña desde hace 30 años a sus oyentes en el regreso a casa.

La Luciérnaga se enciende para hablar de el Gobierno, pues se confirmó que antes de acabar el año se estabilizará el precio de la gasolina, que estará sobre los 15 mil pesos por galón, y comenzará  el alza del ACPM. Así mismo, más de 9.000 personas sufren hambruna por el paro armado del ELN en Chocó, asegura el gobernador Ariel Palacios. El mandatario solicitó incluir nuevamente al Clan del Golfo en los diálogos de paz. También, después de varias reuniones con el sector de taxistas en el país, el Ministerio de Transporte confirmó que en las próximas semanas se revisará y analizará las tarifas del servicio de taxi en el territorio nacional.  
La Luciérnaga, un espacio de humor y opinión de Caracol Radio que acompaña desde hace más de 30 años a sus oyentes en el regreso a casa.

Se detectó un plan criminal, terrorista de acuerdo con la información de inteligencia desde Venezuela por parte del Ejercito de Liberación Nacional para atentar contra la vida del Fiscal General de la Nación. Esto provocó en primer lugar, un rechazo generalizado en todos los estamentos del país así como una reunión de emergencia con el Gobierno Nacional para analizar la información de la Fiscalía. Así mismo, algunos bloqueos se mantuvieron en algunas ciudades del país como; Cúcuta, Medellín y Bucaramanga. Son al menos 1500 taxistas que salieron a las calles y carreteras, de esta manera se adelantaron 27 actividades en 13 municipios de 10 departamentos de Colombia. También, dos proyectos ley que se radicaron en el Congreso de La República, los cuales buscan beneficiar a los integrantes de las Fuerzas Militares como Policía Nacional, estos tuvieron algunos cambios positivos. 

La Luciérnaga, un espacio de humor y opinión de Caracol Radio que acompaña desde hace más de 30 años a sus oyentes en el regreso a casa.

Escuche el programa de este miércoles 25 de octubre. La Luciérnaga, un espacio de humor y opinión de Caracol Radio que acompaña desde hace más de 30 años a sus oyentes en su regreso a casa. 

Escuche el programa de este martes 20 de noviembre. La Luciérnaga, un espacio un espacio de humor y opinión de Caracol Radio que acompaña desde hace más de 30 años a sus oyentes en el regreso a casa.

Escuche el programa de este martes 23 de julio. La Luciérnaga, un espacio de humor y opinión de Caracol Radio que desde hace 31 años acompaña a sus oyentes en su regreso casa.

Hassan Fares, candidato a la Alcaldía de Barranquilla, dio detalles sobre las acusaciones a la Policía.

En Caracol Radio estuvo Gustavo Bolívar, director del Departamento de Prosperidad Social

Julián Sánchez, alcalde de Soacha, habló en 6AM sobre que representa para Soacha convertirse en la primera ciudad que hará transición enrgética en 300 taxis 

A 14-year-old boy is in hospital after being hit by a taxi at Greenacre on Monday.

Ride.bm said they are offering an “easy-to-access app that provides Bermudians and visitors alike with seamless, on-demand transportation.” A spokesperson said, “For decades, the number 295-4141 has been synonymous with safe and reliable transportation in Bermuda. Today, Ride.bm, powered by Central Dispatch, builds on that legacy, transforming a household number into a modern, easy-to-access app […]

Police are appealing for assistance in locating a female who, in what was apparently an intoxicated state, walked away from a taxi driver in the early hours of Saturday morning. A police spokesperson said, “Police responded to an incident that took place at 1am on Saturday February 14th on Harbour Road in the area of […]

BUEI Silver Screens will present ‘Maximum Truth’ starring Dylan O’Brien and Ike Barinholtz, a mockumentary comedy about political grifter Rick Klingman’s quest for truth in Congress. A spokesperson said, “‘BUEI Silver Screens’ continues at the Bermuda Underwater Exploration Institute [BUEI] with the screening of ‘Maximum Truth’ on Tuesday, April 16th at 2 pm in our […]

We’re pleased to note the release of AxiDraw software version 3.9 this week, with a couple of neat new features. One of them is that the “preview mode” button — which lets you simulate plotting to see how the results will come out — is now accessible no matter which function is selected. The other, … Continue reading Hidden line removal for AxiDraw →

Tiny product update: A new version of the pen holder for AxiDraw, with slots for quick adjustment between the vertical and 45 degree angle positions.

With the Sun at the peak of its activity cycle, we Earthlings get treated to some awesome sights. Plus, some truly awe-inspiring launches happened this week.

The rotation curves of disc galaxies are flat and dark matter is speculated as explanation. Alternatively, the gravity of material disk could explain the flat curves. Using the gravitational force that a disk exerts on a body in the disk, we have computed the the rotation curves of disc galaxies and the curve of their...

I do a detailed analysis of Cantor’s theory of uncountable sets. The logic of his proofs has some weaknesses. I propose an axiom and a solution to continuum hypothesis. The main idea is: Assumption of Cantor’s proofs: All real numbers (set R) are in a list (list L). This assumption means R=L, considering L as...

An analysis of Cantor’s theory of uncountable sets: The logic of his proofs has some weaknesses. Cantor assumes for both his proofs that all real numbers (set R) are in a list (list L). Considering L as a set this assumption assumes R belongs to L. This makes the claim “a real number is constructed...

A travel guide for moms who are traveling alone. It can be hard to find time to do things for yourself when you have young kids at home, so why not take advantage of the opportunity to travel without them? Here are some great ideas for your next solo momcation.

The post 9 Tips for Your Best Momcation – How to Maximize your Relaxation appeared first on Joyful Abode.

His style complements the whimsical narrative of a woman taking an unconventional cab ride to the moon.

The Sing Sing maximum security prison in New York held its first-ever film festival recently, with incarcerated men invited to judge the five entries.

The TV presenter says the previous Tory administration was "reckless" to scale back climate policy.

Jürgen Cox
Sep 1, 2014; 13:2513-2526
Technological Innovation and Resources

The molecular mechanisms of reduced frataxin (FXN) expression in Friedreich's ataxia (FRDA) are linked to epigenetic modification of the FXN locus caused by the disease-associated GAA expansion. Here, we identify that SUV4-20 histone methyltransferases, specifically SUV4-20 H1, play an important role in the regulation of FXN expression and represent a novel therapeutic target. Using a human FXN–GAA–Luciferase repeat expansion genomic DNA reporter model of FRDA, we screened the Structural Genomics Consortium epigenetic probe collection. We found that pharmacological inhibition of the SUV4-20 methyltransferases by the tool compound A-196 increased the expression of FXN by ∼1.5-fold in the reporter cell line. In several FRDA cell lines and patient-derived primary peripheral blood mononuclear cells, A-196 increased FXN expression by up to 2-fold, an effect not seen in WT cells. SUV4-20 inhibition was accompanied by a reduction in H4K20me2 and H4K20me3 and an increase in H4K20me1, but only modest (1.4–7.8%) perturbation in genome-wide expression was observed. Finally, based on the structural activity relationship and crystal structure of A-196, novel small molecule A-196 analogs were synthesized and shown to give a 20-fold increase in potency for increasing FXN expression. Overall, our results suggest that histone methylation is important in the regulation of FXN expression and highlight SUV4-20 H1 as a potential novel therapeutic target for FRDA.

Carolyn S. Gordon and Michael R. Jablonski
Trans. Amer. Math. Soc. 377 (), 5673-5704.
Abstract, references and article information

Frederic Heihoff
Proc. Amer. Math. Soc. 152 (), 5229-5247.
Abstract, references and article information

Donghyeok Lim and Christian Maire
Proc. Amer. Math. Soc. 152 (), 5013-5024.
Abstract, references and article information

Visual Abstract

Postoperative recurrence from microscopic residual disease must be prevented to cure intractable cancers, including pancreatic cancer. Key to this goal is the elimination of cancer stem cells (CSCs) endowed with tumor-initiating capacity and drug resistance. However, current therapeutic strategies capable of accomplishing this are insufficient. Using in vitro models of CSCs and in vivo models of tumor initiation in which CSCs give rise to xenograft tumors, we show that dexamethasone induces expression of MKP-1, a MAPK phosphatase, via glucocorticoid receptor activation, thereby inactivating JNK, which is required for self-renewal and tumor initiation by pancreatic CSCs as well as for their expression of survivin, an anti-apoptotic protein implicated in multidrug resistance. We also demonstrate that systemic administration of clinically relevant doses of dexamethasone together with gemcitabine prevents tumor formation by CSCs in a pancreatic cancer xenograft model. Our study thus provides preclinical evidence for the efficacy of dexamethasone as an adjuvant therapy to prevent postoperative recurrence in patients with pancreatic cancer.

NSun2 is an RNA methyltransferase introducing 5-methylcytosine into tRNAs, mRNAs, and noncoding RNAs, thereby influencing the levels or function of these RNAs. Autotaxin (ATX) is a secreted glycoprotein and is recognized as a key factor in converting lysophosphatidylcholine into lysophosphatidic acid (LPA). The ATX-LPA axis exerts multiple biological effects in cell survival, migration, proliferation, and differentiation. Here, we show that NSun2 is involved in the regulation of cell migration through methylating ATX mRNA. In the human glioma cell line U87, knockdown of NSun2 decreased ATX protein levels, whereas overexpression of NSun2 elevated ATX protein levels. However, neither overexpression nor knockdown of NSun2 altered ATX mRNA levels. Further studies revealed that NSun2 methylated the 3'-UTR of ATX mRNA at cytosine 2756 in vitro and in vivo. Methylation by NSun2 enhanced ATX mRNA translation. In addition, NSun2-mediated 5-methylcytosine methylation promoted the export of ATX mRNA from nucleus to cytoplasm in an ALYREF-dependent manner. Knockdown of NSun2 suppressed the migration of U87 cells, which was rescued by the addition of LPA. In summary, we identify NSun2-mediated methylation of ATX mRNA as a novel mechanism in the regulation of ATX.

Natalie Bruiners
Dec 1, 2020; 61:1617-1628
Research Articles

When viewing a SAS Visual Analytics report, you might intermittently see an error that includes content similar to the following:

The rise of drug-resistant tuberculosis poses a major risk to public health. Statins, which inhibit both cholesterol biosynthesis and protein prenylation branches of the mevalonate pathway, increase anti-tubercular antibiotic efficacy in animal models. However, the underlying molecular mechanisms are unknown. In this study, we used an in vitro macrophage infection model to investigate simvastatin’s anti-tubercular activity by systematically inhibiting each branch of the mevalonate pathway and evaluating the effects of the branch-specific inhibitors on mycobacterial growth. The anti-tubercular activity of simvastatin used at clinically relevant doses specifically targeted the cholesterol biosynthetic branch rather than the prenylation branches of the mevalonate pathway. Using Western blot analysis and AMP/ATP measurements, we found that simvastatin treatment blocked activation of mechanistic target of rapamycin complex 1 (mTORC1), activated AMP-activated protein kinase (AMPK) through increased intracellular AMP:ATP ratios, and favored nuclear translocation of transcription factor EB (TFEB). These mechanisms all induce autophagy, which is anti-mycobacterial. The biological effects of simvastatin on the AMPK-mTORC1-TFEB-autophagy axis were reversed by adding exogenous cholesterol to the cells. Our data demonstrate that the anti-tubercular activity of simvastatin requires inhibiting cholesterol biosynthesis, reveal novel links between cholesterol homeostasis, the AMPK-mTORC1-TFEB axis, and Mycobacterium tuberculosis infection control, and uncover new anti-tubercular therapy targets.

Study of workplace stress finds there's a better way to cope than relaxation.

Two lawsuits were filed Monday challenging a proposition that Arizona voters approved to impose an additional 3.5% tax on individuals earning above $250,000 to pay school teacher salaries and training.

Evdokia Menelaou
Aug 8, 2012; 32:10925-10939
BehavioralSystemsCognitive

In about four billion years from now, the Milky Way and Andromeda galaxies will crash together Visualization: NASA, ESA, and F. Summers, STScI Simulation credit: NASA, ESA, G. Besla, Columbia University, and R. van der Marel, STScI

No one was injured in the blast, and authorities are investigating why the ordnance detonated after so many years underground

One small prayer for a taxi leads to a divine encounter.