From climbing walls to a roaming music studio, we've seen a lot of ingenious tiny house features over the years. But this tiny home on wheels from Movable Roots has a distinct feature we never thought was possible — a walk-in hot tub! The Culp is a 500-square-foot home that, in addition to its accessible, spa-like bathroom, boasts unique cork flooring and an incinerating toilet.[...]

Tiny homes are still all the rage within the minimalist and wanderlust communities of the world, and what better place to consider tiny living than in the warmth of the big island, Hawaii? If equatorial location is on your must-have list, this tiny home might be just the serving of simplicity and decadence you’re looking for. [...]

I've been doing my part to keep an eye on my older neighbors, which helps calm my own feelings of fear and helplessness.

Here's a look at homes under 1,000 square feet that are listed for roughly $500,000 in Long Beach, South L.A. and El Sereno in L.A. County.

THE next Destiny 2 Trials of Osiris event is here and now we know a lot more about the loot and rewards available on PS4, Xbox One and PC this week.

IndyStar reporter Domenica Bongiovanni searched for tiny statues hidden by artist Dominic Sansone in 2009 for a Herron art class.

Owen Sego's dad died in a plane crash. Avery Whitten's mom died of heart failure. Two tragedies hit two Indian Creek football players in 24 hours.

The following article, Attorney for Jailed Dallas Salon Owner Shelley Luther Slams Obama Loyalist Judge as a “Tiny Tyrant”, was first published on 100PercentFedUp.com.

Warren Norred, attorney for Shelley Luther, joined Tucker Carlson to discuss the decision by an Obama loyalist Texas judge to jail his client for reopening her salon during the COVID-19 pandemic. Norred said the judge has exposed himself for the “tiny tyrant” that he is. The judge jailed Luther and even fined her $7,000. Texas […]

Continue reading: Attorney for Jailed Dallas Salon Owner Shelley Luther Slams Obama Loyalist Judge as a “Tiny Tyrant” ...

The study of protein subcellular distribution, their assembly into complexes and the set of proteins with which they interact with is essential to our understanding of fundamental biological processes. Complementary to traditional assays, proximity-dependent biotinylation (PDB) approaches coupled with mass spectrometry (such as BioID or APEX) have emerged as powerful techniques to study proximal protein interactions and the subcellular proteome in the context of living cells and organisms. Since their introduction in 2012, PDB approaches have been used in an increasing number of studies and the enzymes themselves have been subjected to intensive optimization. How these enzymes have been optimized and considerations for their use in proteomics experiments are important questions. Here, we review the structural diversity and mechanisms of the two main classes of PDB enzymes: the biotin protein ligases (BioID) and the peroxidases (APEX). We describe the engineering of these enzymes for PDB and review emerging applications, including the development of PDB for coincidence detection (split-PDB). Lastly, we briefly review enzyme selection and experimental design guidelines and reflect on the labeling chemistries and their implication for data interpretation.

Payman Samavarchi-Tehrani
May 1, 2020; 19:757-773
Review

The study of protein subcellular distribution, their assembly into complexes and the set of proteins with which they interact with is essential to our understanding of fundamental biological processes. Complementary to traditional assays, proximity-dependent biotinylation (PDB) approaches coupled with mass spectrometry (such as BioID or APEX) have emerged as powerful techniques to study proximal protein interactions and the subcellular proteome in the context of living cells and organisms. Since their introduction in 2012, PDB approaches have been used in an increasing number of studies and the enzymes themselves have been subjected to intensive optimization. How these enzymes have been optimized and considerations for their use in proteomics experiments are important questions. Here, we review the structural diversity and mechanisms of the two main classes of PDB enzymes: the biotin protein ligases (BioID) and the peroxidases (APEX). We describe the engineering of these enzymes for PDB and review emerging applications, including the development of PDB for coincidence detection (split-PDB). Lastly, we briefly review enzyme selection and experimental design guidelines and reflect on the labeling chemistries and their implication for data interpretation.

A small, isolated country, Iceland has been home to a largely homogenous population for much of its history. But in recent years, a booming economy and expanding tourism sector have drawn rising numbers of immigrants to the island nation. This article explores Iceland's balancing act of maintaining economic growth through immigration while preserving its culture and language.