To overcome these challenges, researchers are exploring “test-time compute,” a technique that enhances existing AI models during the so-called “inference” phase, or when the model is being used

Researchers experiment with area-selective atomic layer deposition to precisely place layers of conducting and insulating materials within circuits

Large and small suppliers vow to help a resource-intensive, cost-sensitive industry change with the times

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Hayden Library - TA418.9.N35 F87 2018

β-1,3-d-Glucan is a ubiquitous glucose polymer produced by plants, bacteria, and most fungi. It has been used as a diagnostic tool in patients with invasive mycoses via a highly-sensitive reagent consisting of the blood coagulation system of horseshoe crab. However, no method is currently available for measuring β-1,6-glucan, another primary β-glucan structure of fungal polysaccharides. Herein, we describe the development of an economical and highly-sensitive and specific assay for β-1,6-glucan using a modified recombinant endo-β-1,6-glucanase having diminished glucan hydrolase activity. The purified β-1,6-glucanase derivative bound to the β-1,6-glucan pustulan with a KD of 16.4 nm. We validated the specificity of this β-1,6-glucan probe by demonstrating its ability to detect cell wall β-1,6-glucan from both yeast and hyphal forms of the opportunistic fungal pathogen Candida albicans, without any detectable binding to glucan lacking the long β-1,6-glucan branch. We developed a sandwich ELISA-like assay with a low limit of quantification for pustulan (1.5 pg/ml), and we successfully employed this assay in the quantification of extracellular β-1,6-glucan released by >250 patient-derived strains of different Candida species (including Candida auris) in culture supernatant in vitro. We also used this assay to measure β-1,6-glucan in vivo in the serum and in several organs in a mouse model of systemic candidiasis. Our work describes a reliable method for β-1,6-glucan detection, which may prove useful for the diagnosis of invasive fungal infections.

The enactment of President Clinton’s Limited English Proficiency (LEP) Executive Order, issued in 2000, triggered a proliferation of efforts to provide services to individuals who cannot speak, understand, read, or write English fluently. With increased service provision, state and local government agencies have expressed a strong and growing interest in assuring the quality and cost-effectiveness of language access services. This paper attempts to catalog and describe some of those tools and practices.

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When economic shocks occur, economic agents are expected to react to reduce the negative impact or amplify the positive effects. The ability of a country to contain economic losses can be defined as the resilience to economic shocks. Using the OECD Inter-Country Input-Output tables, this paper investigates the relationship between changes in final demand and production structures for 61 economies.

The OECD Advisory Group on Endocrine Disrupters Testing and Assessment met on 16-17 October 2014 in Paris to discuss the development and update of Test Guidelines and related documents for endocrine disrupters testing and assessment.

This guidance is intended to harmonise the way non-guideline in vitro test methods are described. This should in future facilitate an assessment of the relevance of test methods for biological activities and responses of interest, of the quality of data produced, irrespective of whether these tests are based on manual protocols or assay protocols adapted for use on automated platforms or high-throughput screening systems (HTS).

This new document focuses on the evaluation of test methods applied to determine the physico-chemical properties of different types of nanomaterials.

Participants from 15 countries attended the Workshop on Developmental Neurotoxicity: The use of non-animal test methods for regulatory purposes” on 18 October 2016, in Belgium. The event, co-organised by the OECD and the European Food Safety Authority (EFSA), focused on opportunities and challenges related to alternative methods for testing and assessing the DNT potential of chemicals.

As of April 24, 2020, the total number of confirmed COVID-19 cases in India is 23,077 including 4,749 recovered cases and 718 deaths. Seeing a 19-days incubation period of the virus, medical experts have suggested 14 days quarantine for people who

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If you are wondering when to apply which design methods, this mind map may help you.

Small-animal physiology studies are typically complicated, but the level of complexity is greatly increased when performing live-animal X-ray imaging studies at synchrotron and compact light sources. This group has extensive experience in these types of studies at the SPring-8 and Australian synchrotrons, as well as the Munich Compact Light Source. These experimental settings produce unique challenges. Experiments are always performed in an isolated radiation enclosure not specifically designed for live-animal imaging. This requires equipment adapted to physiological monitoring and test-substance delivery, as well as shuttering to reduce the radiation dose. Experiment designs must also take into account the fixed location, size and orientation of the X-ray beam. This article describes the techniques developed to overcome the challenges involved in respiratory X-ray imaging of live animals at synchrotrons, now enabling increasingly sophisticated imaging protocols.

Innovative new crystallographic methods are facilitating structural studies from ever smaller crystals of biological macromolecules. In particular, serial X-ray crystallography and microcrystal electron diffraction (MicroED) have emerged as useful methods for obtaining structural information from crystals on the nanometre to micrometre scale. Despite the utility of these methods, their implementation can often be difficult, as they present many challenges that are not encountered in traditional macromolecular crystallography experiments. Here, XFEL serial crystallography experiments and MicroED experiments using batch-grown microcrystals of the enzyme cyclophilin A are described. The results provide a roadmap for researchers hoping to design macromolecular microcrystallography experiments, and they highlight the strengths and weaknesses of the two methods. Specifically, we focus on how the different physical conditions imposed by the sample-preparation and delivery methods required for each type of experiment affect the crystal structure of the enzyme.

Recent developments have resulted in electron cryo-microscopy (cryo-EM) becoming a useful tool for the structure determination of biological macromolecules. For samples containing inherent flexibility, heterogeneity or preferred orientation, the collection of extensive cryo-EM data using several conditions and microscopes is often required. In such a scenario, merging cryo-EM data sets is advantageous because it allows improved three-dimensional reconstructions to be obtained. Since data sets are not always collected with the same pixel size, merging data can be challenging. Here, two methods to combine cryo-EM data are described. Both involve the calculation of a rescaling factor from independent data sets. The effects of errors in the scaling factor on the results of data merging are also estimated. The methods described here provide a guideline for cryo-EM users who wish to combine data sets from the same type of microscope and detector.

The sin2ψ and cosα methods are compared via diffracting-grain identification from electron backscatter diffraction maps. Artificial textures created by the X-ray diffraction measurements are plotted and X-ray elastic constants of the diffracting-grain sets are computed.

The most widely used methods for calculating species extinction rates are "fundamentally flawed" and overestimate extinction rates by as much as 160 percent, life scientists report May 19 in the journal Nature.

The post Methods for calculating species extinction rates overestimate extinction, says Smithsonian scientist appeared first on Smithsonian Insider.

Oysters are keystone organisms in estuaries around the world, influencing water quality, constructing habitat and providing food for humans and wildlife. Yet their populations in […]

The post Ancient Native-American methods may be key to sustainable oyster harvests appeared first on Smithsonian Insider.

The standard method of joint probability distribution functions, so crucial for the development of direct methods, has been revisited and updated. It consists of three steps: identification of the reflections which may contribute to the estimation of a given structure invariant or seminvariant, calculation of the corresponding joint probability distribution, and derivation of the conditional distribution of the invariant or seminvariant phase given the values of some diffracted amplitudes. In this article the conditional distributions are derived directly without passing through the second step. A good feature of direct methods is that they may work in the absence of any prior information: that is also their weakness. Different types of prior information have been taken into consideration: interatomic distances, interatomic vectors, Patterson peaks, structural model. The method of directly deriving the conditional distributions has been applied to those cases. Some new formulas have been obtained estimating two-, three- and four-phase invariants. Special attention has been dedicated to the practical aspects of the new formulas, in order to simplify their possible use in direct phasing procedures.

Several strong earthquakes around the world have resulted in a phenomenon called soil liquefaction, the seismic generation of excess porewater pressures and softening of granular soils, often to the point that they may not be able to support the foundations of buildings and other infrastructure.

Although individual anglers – people who fish recreationally – generally take small numbers of fish, collectively, a large number of them can have a substantial impact on the overall stock.

A new report from the National Academies of Sciences, Engineering, and Medicine outlines how to examine whether specific levels of nutrients or other food substances (NOFSs) can ameliorate the risk of chronic disease and recommends ways to develop dietary reference intakes (DRI) based on chronic disease outcomes.

Producing more precise county-level estimates of crops and farmland cash rents will require integrating multiple data sources using model-based predictions that are more transparent and reproducible, says a new report from the National Academies of Sciences, Engineering, and Medicine.

Using 'gardening' techniques to actively restore endangered coral reefs is ecologically sound and economically feasible, according to recent research.

The presence of plastics in aquatic environments is a growing concern across the EU. This study explored the amount of microplastic particles present in raw and treated water at three water-treatment plants in the Czech Republic. While treated water contained fewer particles than raw1 fresh water, the amount found in treated water was not negligible, and largely comprised tiny particles of <10 micrometres (μm) in diameter. Ways to filter microplastics from potable water must be identified and their risk to humans, sources and routes into drinking water determined, say the researchers.

It is difficult to compare estimates of noise exposure across EU Member States because the methods used to produce the data vary between countries. A new study has investigated five methods of estimating noise exposure and identified some of the reasons for variation in the data they produce.

Methods for assessing the sustainability of tourist destinations have been developed. In a new Spanish study, researchers describe a framework for analysing sustainability that is designed for policymakers and tourism managers, and, as a case study, apply it to the region of Andalusia.

A three-step chemical process could successfully recover high-purity silicon from recycled photovoltaic (PV) solar panels, new tests show. The scientists behind the research say that recycling not only helps the PV industry meet regulatory requirements, but also reduces pressure on demand for raw materials.

Shortages of indium, a key metal found in thin-film solar cells, could limit their large-scale deployment in the future. A new study has outlined four ways that indium supplies could be increased to meet future demand. For example, indium could be extracted more efficiently from zinc ores, or historic wastes containing indium could be processed to extract the element.

Some 'intangible' ecosystem services, such as the aesthetic and cultural value, as well as the recreational use of land, are particularly difficult to price. New research has proposed three explorative methods to value these more intangible services using landscape evaluation, statistics on level of recreational use and the price of real estate in the area.

The drive to increase renewable energy production can sometimes be at loggerheads with the desire to preserve natural landscapes. In this study, researchers from across Europe assessed the environmental impacts of renewable energies in the Alps, making key recommendations to resolve conflicts between different users of habitats.

When broken down, ships can release hazardous substances into the environment. This study investigated the environmental impact of shipbreaking in one of Europe’s few ship recycling yards, based in Portugal. The results reveal large differences between assessment methods and show that environmental impact depends on composition rather than size or class.