COVID-19 is not an equal opportunity pandemic. As Memorial president Vianne Timmons writes in this guest column, women are often in harm's way because of their work.

Eine Bibel-App bietet Tausenden von Kurden Zugang zu Gottes Wort.

Buenos Aires, Argentinen :: Die Mannschaft der Logos Hope zeigt mit Jugend mit einer Mission alten Menschen Gottes Liebe

Mar del Plata, Argentinien :: Eine Gemeinde hört von Ländern, die keinen Zugang zum Evangelium haben

A new study facilitated by the Workplace Safety and Insurance Board of Ontario states miners who were forced to inhale an aluminum powder were found to have a higher risk of Parkinson’s disease.

As the province of Ontario starts to relax some COVID-19 restrictions, one Sudbury business owner says it feels great to be opening back up.

Business owners throughout northern Ontario will have the chance Friday morning to speak directly with federal Economic Development Minister Mélanie Joly.

Every year Washingtonians are treated to a feast for the eyes as ornamental cherry trees bloom across the city, most prominently by the Tidal Basin. As cherry blossom season approaches, we would like to share information about two related resources that we hope will offer some inspiration for those near and far: a selected set […]

Successful modification of the bacterial artificial chromosome (BAC) after two-step BAC engineering is confirmed in two separate polymerase chain reactions (PCRs). The first reaction (5' co-integrate PCR) uses a forward 5' co-integrate primer (a sequence located upstream of the 5' end of the A-box) and a reverse 3' primer on the vector (175PA+50AT) or within the reporter sequence or mutated region as appropriate. The second reaction (3' co-integrate PCR) uses a forward 5' primer on the recA gene (RecA1300S) and a reverse 3' co-integrate primer (a sequence located downstream from the 3' end of the B-box). Those colonies shown to be positive in PCR analysis are further tested for sensitivity to UV light. After the resolution, colonies that have lost the excised recombination vector including sacB and recA genes become UV light sensitive.

Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.

Plasmid DNA is prepared from the recombinant shuttle vector pLD53.SCAB/A-B created by cloning of the A and B homology arms for two-step bacterial artificial chromosome (BAC) engineering. To confirm that the A-box and B-box arms have been successfully incorporated into pLD53.SCAB, the pattern of enzyme digestion of the modified plasmid is compared with that of the unmodified pLD53.SCAB. Once the shuttle vector is shown to carry the proper sequences, it is ready for transfer into the BAC host.

This protocol describes the preparation of the shuttle vector before its introduction into bacterial artificial chromosome (BAC) host cells for BAC two-step engineering. The homology arm sequences, prepared previously, are introduced by ligation into the digested shuttle vector DNA to provide sites for recombination within the BAC clone. Crude lysates of individual bacterial transformants serve as templates in polymerase chain reaction (PCR) analysis to confirm the presence of the homology arms in the recombinant shuttle vector.

The 700-bp A homology arm (A-box) and the 700-bp B homology arm (B-box) are amplified by polymerase chain reaction (PCR) using purified bacterial artificial chromosome (BAC) DNA as template for two-step BAC engineering. The resulting A-box PCR product contains an AscI site at its 5' end (the 5' primer incorporates an AscI site, and the 3' primer does not incorporate any restriction sites). The B-box PCR product contains an XmaI site at its 3' end (the 5' primer does not incorporate any restriction sites, and the 3' primer incorporates an XmaI site). The amplification products are then digested with the appropriate restriction endonucleases to render them suitable for cloning into the shuttle vector.

In two-step bacterial artificial chromosome (BAC) engineering, a single plasmid is introduced into the BAC-carrying cell lines. The shuttle vector pLD53.SCAB (or pLD53.SCAEB) carries the recA gene and the R6K origin, which requires the protein to replicate. PIR2 cells, expressing , are typically used for the amplification of the vector and maintain about 15 copies/cell of the donor vector, which is relatively stable in this host.

Recent literature has highlighted that international trade is mostly priced in a few key vehicle currencies, and is increasingly dominated by intermediate goods and global value chains (GVCs). Taking these features into account, this paper reexamines the business cycle dynamics of international trade and its relationship with monetary policy and exchange rates.

A prototype deployed in San Francisco Bay imagines the underside of a floating building as an upside-down artificial reef

A new book explores the evolution of cartography throughout more than a century of commercial air travel

Tissue engineering has a unique set of tools and technologies for developing preventive strategies, diagnostics, and treatments that can play an important role during the ongoing COVID-19 pandemic.

Businesses in Cape Breton are helping customers get creative with DIY take-home kits to make everything from mermaid-themed terrariums to bubble tea.

What would have been Cape Breton University's largest convocation to date is being replaced by an online offering this year because of COVID-19. Some graduating students say it just won't be the same as a traditional ceremony.

For those working in CHSLDs — the province's long-term care homes which are the site of the most serious outbreaks — the bonus will amount to as much as $1,000 per month.

For close to 15 years, Reema Singh has been baking and selling cakes from her tiny shop on Parc Avenue in Montreal's Mile End. Cocoa Locale has been open throughout the pandemic because — well, it turns out that cake is essential.

The current deconfinement plan in the Montreal area could lead to a rapid increase in deaths in the city, according to findings by Quebec's public health institute. 

Sheltering in place doesn’t mean you have to give up the best of wine country's offerings

Overcoming scorching heat and little rain, experimental vineyards teach winemakers to cope with climate change

A dead body was found along the CP Rail line near Molson, Manioba, RCMP say.

With Mother's Day only a few days away, businesses are coming up with creative ways to help your family celebrate. Everything from brunch in a box to colourful bouquets and home delivered chocolate.

They're not necessarily treating sick patients in hospitals, but a number of Manitoba-based scientists are working long hours and facing incredible pressure to battle the novel coronavirus from their labs and research facilities.

As a result of the pandemic, USPS, which has run at a loss for years, is even more cash-strapped. It expects to lose $2 billion each month during the pandemic. That prompted Postmaster General Megan Brennan to ask Congress for $50 billion in funds -- $25 billion to offset lost revenue from declining mail volume due to the pandemic, and another $25 billion for modernization.

Salesforce has announced a new version of Work.com designed to help businesses function safely during the COVID-19 pandemic. "Work.com is a completely new initiative using an existing domain name that we previously owned," said Salesforce spokesperson Joel Steinfeld. "Our focus is on speed and moving as quickly as possible to help our customers, and Work.com is an optimal way to do that.

Sign makers in P.E.I. have been busy since the province announced its plans to ease back COVID-19 restrictions, as businesses are ordering signs and decals ahead of reopening.

Nearly 5 ½ years ago, the water source for the city of Flint was switched in an effort to save money. A disaster followed resulting in deaths, illness and mistrust of government. Tuesday at 10:00 p.m. on WKAR-TV, the documentary series Frontlin e examines the crisis by bringing to light conversations and documents never seen on television before. We spoke with Abby Ellis, the Michigan native who is the film’s director.

Almost every movie reminds Trump of … something it’s not.

Gold and silver prices often move in tandem, but the gap between them widened by 31% January through April in line with a trend that began nine years ago.

How to rebuild social solidarity.

Interview with Ms Christine Lagarde, President of the European Central Bank, and Le Parisien, conducted by Mr Matthieu Pelloli and published on 9 April 2020.

Interview with Ms Christine Lagarde, President of the European Central Bank, and France Inter, conducted by Mr Ali Baddou and Ms Carine Bécard on 9 April 2020.

Statement by Ms Christine Lagarde, President of the European Central Bank, at the forty-first meeting of the International Monetary and Financial Committee, IMF Virtual meetings, Washington DC, 16 April 2020.

The upcoming debate about whether to expand the urban boundary is one of the city's big issues this term. But instead of delaying it, the city's testing out a never-before-attempted democratic process. Should they?

Ice dancers Piper Gilles and Paul Poirier headline the Canadian team that will compete at this year's world figure skating championships, but Skate Canada is waiting until next month to make a final decision on several of their teammates.

For Canadian champion Roman Sadovsky and former Canadian champions Nam Nguyen and Keegan Messing, the Four Continents results will determine who takes the one spot available in the men's event at the worlds.

Canada's Piper Gilles and Paul Poirier won the silver medal in ice dancing on Friday at the ISU Four Continents figure skating competition in Seoul, South Korea.

Canadians Kirsten Moore-Towers and Michael Marinaro won the bronze medal in pairs on Saturday at the ISU Four Continents figure skating competition in Seoul, South Korea.

The Canadian four-time world champion figure skater took part in the Americares Blades for the Brave fundraiser for front-line workers battling the COVID-19 pandemic.

Canadian figure skater Scott Moir posted a video on Instagram and Twitter on Thursday, explaining that he and fiancee Jackie Mascarin are currently stationed in Tampa, Fla., because Mascarin works as a physician's assistant in the respiratory unit of a local hospital.

Grit and red wine: Famous war photographer beats virus at 97  WSET

Bates: McHenry businesses adapt to COVID-19  Northwest Herald