ign Repeat Signage designer screen and controls By www.repeatsoftware.com Published On :: Sat, 12 Feb 2022 12:50:51 GMT 7-minute video shows you how to use the Repeat Signage designer screen to insert controls, e.g. picture control, to create flexible, dynamic, digital signage presentations for display screens. Full Article
ign 5 skills your web designer should have By ignitionmedia.com.au Published On :: Tue, 11 Oct 2016 04:05:50 +0000 In order for your business to have a professional presence online, you must seek the services of a... The post 5 skills your web designer should have appeared first on Ignition Media. Full Article Website Design Css mobile design Mobile responsive SEO Web Design Web Designer
ign Why It Pays to Invest in your Website Design By ignitionmedia.com.au Published On :: Tue, 18 Oct 2016 03:54:19 +0000 As a small business owner, regardless of what business you are in, you need to have a great... The post Why It Pays to Invest in your Website Design appeared first on Ignition Media. Full Article Website Design Design Trends
ign 10 Ignition Media Tactics to Take Your Website to the Next Level By ignitionmedia.com.au Published On :: Tue, 19 Jul 2022 22:00:51 +0000 The website is the foundation of any successful online business. It’s the first impression potential customers have of... The post 10 Ignition Media Tactics to Take Your Website to the Next Level appeared first on Ignition Media. Full Article Website Design
ign The Top Website Design Trends for 2024 By ignitionmedia.com.au Published On :: Sat, 30 Dec 2023 22:18:22 +0000 At Ignition Media, as we embrace 2024, we’re keenly aware of the evolving trends in web design that... The post The Top Website Design Trends for 2024 appeared first on Ignition Media. Full Article Website Design
ign 7 Bad Signs for Democrats: A Satirical Take on America’s Return to Normalcy By theblacksphere.net Published On :: Sat, 09 Nov 2024 04:24:17 +0000 The following article, 7 Bad Signs for Democrats: A Satirical Take on America’s Return to Normalcy, was first published on The Black Sphere. Yes, the Democrats got shellacked in the last election. And while that’s a breath of fresh air, it’s hardly the only sign that the political winds are changing. Continue reading 7 Bad Signs for Democrats: A Satirical Take on America’s Return to Normalcy ... Full Article Featured News
ign Smear campaign underway against DeSantis’ sheriff pick By shark-tank.com Published On :: Mon, 04 May 2020 18:10:37 +0000 For whatever reason, every election year Broward County always manages to steal the show when it comes to shady campaign practices. The 2020 election cycle is no different, and we are not referring to the embarrassment that is the Broward Republican Executive Committee, yet but there is still plenty of time for that group of misfits to step in it. The post Smear campaign underway against DeSantis’ sheriff pick appeared first on Shark Tank. Full Article Featured Florida Politics flordia florida florida politics gregory tony ron desantis shark tank
ign Kari Lake’s Campaign Chair Reacts to Election: “No Transparency” (Video) By sonsoflibertymedia.com Published On :: Tue, 12 Nov 2024 19:16:53 +0000 For a second time, Kari Lake has been denied a win in an election. First, it was for governor of Arizona, which was clearly due to election and voter fraud. Now, it appears something similar occurred in her race against Ruben Gallego. Now, the head of her campaign is speaking out and saying that there … Full Article News U.S. News Videos
ign Biden’s Signature Climate ‘Boondoggle’ Might Be On Chopping Block After Trump Win By libertyunyielding.com Published On :: Sun, 10 Nov 2024 21:09:43 +0000 By David Blackmon In the wake of the election of President Donald Trump to serve a second term in office, along with presumptive Republican majorities in both houses of Congress, many are now asking about what the future will hold for the oddly named Inflation Reduction Act. Trump made it repeatedly clear on the campaign […] The post Biden’s Signature Climate ‘Boondoggle’ Might Be On Chopping Block After Trump Win appeared first on Liberty Unyielding. Full Article Business Law and Government
ign When Amorim played with a broken arm to reignite football dream By www.bbc.com Published On :: Sat, 02 Nov 2024 08:54:57 GMT From rejection at Benfica to new Manchester United head coach, BBC Sport charts the rise of Ruben Amorim. Full Article
ign Sauber sign rookie Bortoleto for 2025 season By www.bbc.com Published On :: Wed, 06 Nov 2024 09:24:23 GMT Gabriel Bortoleto, 20, will make his Formula One debut for the Audi-owned Sauber next season. Full Article
ign Why Kamala Harris lost: A flawed candidate or doomed campaign? By www.bbc.com Published On :: Thu, 07 Nov 2024 01:13:37 GMT The vice-president failed to make the case that she would be different from her boss, President Joe Biden. Full Article
ign Trump At Jacksonville Mocks Biden As ‘Lowest Energy Individual’ For Calling Campaign ‘Lid This Morning Again’ By hispolitica.com Published On :: Fri, 25 Sep 2020 03:34:09 +0000 President Trump mocked Joe Biden Thursday evening for always calling a “lid” on campaign activities early in the morning, saying the Democratic presidential rival is “the lowest energy individual I’ve ever seen.” “Did you see [Biden] did a lid this morning again?” Trump told supporters during his campaign rally Thursday night at Cecil Airport hanger in Jacksonville, […] The post Trump At Jacksonville Mocks Biden As ‘Lowest Energy Individual’ For Calling Campaign ‘Lid This Morning Again’ appeared first on Hispolitica. Full Article 2020 Election 2020 2020 elections 2020 Presidential Race Andrew Bates Biden Campaign florida Jacksonville Joe Biden kamala harris Lid president trump trump rally
ign Biden Campaign Rejects Trump Campaign Calls For Pre-Debate Inspection For Electronic Earpieces By hispolitica.com Published On :: Tue, 29 Sep 2020 17:58:12 +0000 The Trump campaign wants a third-party inspector to inspect both President Trump and Democratic presidential nominee Joe Biden ears for electronic devices or transmitters prior to Tuesday night’s presidential debate. Trump has consented to the inspection while the Biden campaign has declined. However, according to a source told Floridian Press that the Biden campaign agreed […] The post Biden Campaign Rejects Trump Campaign Calls For Pre-Debate Inspection For Electronic Earpieces appeared first on Hispolitica. Full Article 2020 Election 2020 2020 election 2020 Presidential Race Chris Wallace Cleveland Earpieces fox news Joe Biden Kate Bedingfield president trump Presidential Debate Tim Murtaugh Trump Campaign
ign Trump Campaign Launches ‘Operation MAGA’ By hispolitica.com Published On :: Sun, 04 Oct 2020 07:10:42 +0000 Trump 2020 reelection campaign announced Saturday for the final month before Election Day the launch of “Operation MAGA,” that will consist of a “full marshaling of top-level surrogates, campaign coalitions and Trump supporters to rally behind the president and carry the campaign forward” until President Trump recovers from COVID-19 and can return on the campaign […] The post Trump Campaign Launches ‘Operation MAGA’ appeared first on Hispolitica. Full Article 2020 Election 2020 2020 elections 2020 Presidential Race Bill Stepien Black Voices For Trump Coronavirus covid-19 Donald Trump Jr. election day Eric Trump Joe Biden Lara Trump Latinos For Trump MAGA MAGA Rally Melania Trump Mike Pence Operation MAGA president trump Trump 2020 Campaign Vice Presidential Debate Women for Trump Youtube
ign 2 ‘Moody’ Home Design Trends Generating Buzz By www.nar.realtor Published On :: Mon, 19 Aug 2024 13:11:44 +0000 2 ‘Moody’ Home Design Trends Generating Buzz Dark interiors are on trend in these two eye-turning styles. By: Melissa Dittmann Tracey Design & Architecture, Design Trends Full Article
ign Archbishop of Canterbury Resigns By www.bbc.co.uk Published On :: Tue, 12 Nov 2024 19:02:00 GMT Justin Welby has stepped down over a church abuse scandal. Full Article
ign Abuse survivors call for further Church of England resignations By www.bbc.com Published On :: Wed, 13 Nov 2024 08:28:58 GMT The Archbishop of Canterbury has announced he will step down over a damning report into abuse. Full Article
ign Protesters call on Valencia governor to resign after deadly floods By www.bbc.com Published On :: Sat, 09 Nov 2024 21:20:38 GMT People are angry at the authorities' handling of floods which killed more than 200 people. Full Article
ign Typical Democrat: Harris Raised One BILLION for her Campaign… Ended it $20 Million In Debt By lidblog.com Published On :: Sat, 09 Nov 2024 00:36:46 +0000 In what seems like the work of a typical Democrat, Kama Harris raised one billion dollars for her campaign… and finished it $20 million in debt. Imagine what she would have done to the United States which would have started out bankrupt if Harris had entered the White House. How do you have an actual […] The post Typical Democrat: Harris Raised One BILLION for her Campaign… Ended it $20 Million In Debt appeared first on The Lid. Full Article Election 2024
ign Biden’s Corrupt FEMA Told Workers Not to Help Hurricane Victims Who Had Trump Signs By lidblog.com Published On :: Mon, 11 Nov 2024 01:18:38 +0000 Joe Biden’s corrupt Federal Emergency Management Administration (FEMA) has been caught telling its on-the-ground operatives not to help anyone with a Donald Trump campaign sign in their yard. The news broke late last week when a whistleblower revealed agency messages that told workers to refuse to help Trump supporters in the wake of Hurricane Milton […] The post Biden’s Corrupt FEMA Told Workers Not to Help Hurricane Victims Who Had Trump Signs appeared first on The Lid. Full Article Crime GOVERNMENT OVEREACH
ign News24 | Archbishop of Canterbury Justin Welby announces resignation over abuse scandal By www.news24.com Published On :: Tuesday Nov 12 2024 19:19:02 The leader of the world's Anglican communion, Archbishop of Canterbury Justin Welby, announced Tuesday he was resigning following a damning report that concluded the Church of England covered up a serial abuse case. Full Article
ign velocityconf: Who's up for a 5 minute lightning talk. Last day to get your #Ignite proposal in for #velocityconf CA. http://t.co/pzL0WJtQH5 By twitter.com Published On :: Wed, 29 May 2013 14:11:50 +0000 velocityconf: Who's up for a 5 minute lightning talk. Last day to get your #Ignite proposal in for #velocityconf CA. http://t.co/pzL0WJtQH5 Full Article
ign China's renaming of its regional jet is another clear sign its homegrown planemaker is coming for Boeing and Airbus By www.businessinsider.com Published On :: Wed, 13 Nov 2024 07:15:46 +0000 The ARJ21 is now to be known as the C909. The renaming plays into COMAC's ambition to enter the global market. Full Article Transportation International china airbus boeing comac c919 c909 aviation
ign Biden’s Corrupt FEMA Told Workers Not to Help Hurricane Victims Who Had Trump Signs By conservativefiringline.com Published On :: Mon, 11 Nov 2024 16:15:51 +0000 The following article, Biden’s Corrupt FEMA Told Workers Not to Help Hurricane Victims Who Had Trump Signs, was first published on Conservative Firing Line. Joe Biden’s corrupt Federal Emergency Management Administration (FEMA) has been caught telling its on-the-ground operatives not to help anyone with a Donald Trump campaign sign in their yard. The news broke late last week when a whistleblower revealed agency messages that told workers to refuse to help Trump supporters in the wake of Hurricane Milton … Continue reading Biden’s Corrupt FEMA Told Workers Not to Help Hurricane Victims Who Had Trump Signs ... Full Article Politics aid FEMA fired Florida Helene North Carolina Trump signs
ign Campaign Door-Knocker Caught Going Full Racist On Camera — Guess Which Party? By clashdaily.com Published On :: Mon, 04 Nov 2024 10:00:22 +0000 For years, the Dems and their media enablers have complained about the 'divisive' nature of politics, and invariably laid blame on the 'other' party. The post Campaign Door-Knocker Caught Going Full Racist On Camera — Guess Which Party? appeared first on Clash Daily. Full Article News Clash
ign YIKES: Kamala’s Failed Campaign Finished DEEP In Debt — Here’s The 411 By clashdaily.com Published On :: Thu, 07 Nov 2024 09:47:09 +0000 It was just a couple of weeks ago that the media was bragging about Kamala's HUGE fundraising haul. The post YIKES: Kamala’s Failed Campaign Finished DEEP In Debt — Here’s The 411 appeared first on Clash Daily. Full Article News Clash
ign Kamala's Campaign Is Still Aggressively Shaking Down Supporters For Cash By www.westernjournal.com Published On :: Tue, 12 Nov 2024 11:00:27 +0000 Even after her loss on Nov. 5, Vice President Kamala Harris’ election campaign is still hounding donors for money. Harris’ campaign has bombarded supporters with fundraising messages following her election […] The post Kamala's Campaign Is Still Aggressively Shaking Down Supporters For Cash appeared first on The Western Journal. Full Article News 2024 election Democratic National Committee Democratic Party Democrats Kamala Harris U.S. News WJ Wire
ign Watch: Oprah Pressed Over Claims That Kamala Harris' Campaign Paid Her $1M for Political Endorsement By www.westernjournal.com Published On :: Tue, 12 Nov 2024 14:35:53 +0000 It was one of the high points in the early days of the Kamala Harris campaign, the honeymoon period where the vice president — newly minted as the Democratic nominee […] The post Watch: Oprah Pressed Over Claims That Kamala Harris' Campaign Paid Her $1M for Political Endorsement appeared first on The Western Journal. Full Article News 2024 election Campaign Campaign finance Celebrities Democrats Hollywood Kamala Harris Oprah Winfrey
ign Investigation: Waste of the Day – Walz Campaign Donors Received $15 Billion in State Business By deneenborelli.com Published On :: Tue, 08 Oct 2024 18:13:14 +0000 Investigation by Jeremy Portnoy originally published by RealClearInvestigations and RealClearWire Topline: Minnesota Gov. Tim Walz accepted $890,000 in campaign donations from employees – including C-suite executives – of 434 state vendors between 2019 and 2022, a new report from OpenTheBooks found. Those same companies collected nearly $15 billion in payments from the state between 2019 … Full Article Commentaries News
ign China's Party Congress: a dose of foreign policy realism is needed By www.chathamhouse.org Published On :: Tue, 11 Oct 2022 14:01:32 +0000 China's Party Congress: a dose of foreign policy realism is needed The World Today mhiggins.drupal 11 October 2022 In the final article of three on the 20th Chinese Communist Party Congress, which opens on 16 October, Yu Jie argues that Beijing must show more pragmatism about Taiwan and the West. How important is foreign policy at the congress? The political reports delivered to the delegates of the week-long 20th Chinese Communist Party Congress, which happens every five years, follow a Marxist-Leninist formula. Economics and the means of production form the base, while politics and society fill in the superstructure. We can expect sections on the work of the Chinese Communist Party (CCP), ideological discussion, economics, domestic politics, foreign affairs, cultural reform and social developments. Usually, the report prioritizes big domestic political issues. The political report typically sheds some light on the status of, and relationships between, senior party members. It can also provide insights into the political fortunes of various interest groups. The report acts as a summary of the party’s achievements and its plans – expressed as the lowest common denominator of consensus between competing factions. The congress will address foreign affairs issues with long-lasting implications for the rest of the world This year’s congress should be no different, and the political reshuffle that takes place is likely to signal how Beijing intends to rise to the many challenges at home and abroad. Given China’s growing power and its fraught relationship with the West, this year’s congress is expected to feature serious discussion on weighty foreign affairs issues affecting Beijing, and which will have long-lasting implications for the rest of the world. Which geopolitical issues will be priorities? When it comes to foreign affairs, China’s priorities rarely change. The CCP seeks to create a stable external environment to foster its domestic economic development. This conservative maxim was advocated in the 1980s by China’s then-paramount leader, Deng Xiaoping, and it will continue to guide Beijing’s relationships after this congress. While we don’t know the exact details of this year’s political report, we can expect discussion on relations with the US-led West, a possible shift in the relationship between Beijing and Moscow, and elaboration on China’s ties with the Global South. Notably, it’s likely that a separate chapter of the political report will see Taiwan treated alongside the special administrative regions of Hong Kong and Macau, signalling the party’s commitment to its claims over the contested island. Despite a chorus of nationalistic rhetoric surrounding the issue of Taiwan, Beijing will be careful not to stumble into an international conflict which risks causing colossal damage on all fronts. The choice of language on the so-called ‘Taiwan question’ in this political report will serve as a bellwether as to how, if at all, the party might fundamentally shift its views regarding Taiwan and deviate from the principle of ‘peaceful reunification’, the policy stated at every congress since 1979. How have China’s relations with the West worsened? President Richard Nixon’s visit to China in 1972, the first by an American president to the Communist nation, kicked off five decades of relative stability. However, as China’s global influence has grown, so have Washington’s fears. US-China relations, once stable and cooperative, are now volatile and competitive The relationship has transitioned from the cooperation and relative stability that existed under President George W. Bush and President Hu Jintao in the early 2000s, into one characterized by volatility and competition under Xi Jinping, Joe Biden and Donald Trump. These tensions are almost certain to continue in Xi’s likely third term. Elements of China’s relationship with the West, such as cooperation over military and aviation technology, are becoming far more competitive. At the same time, trade and investment, once viewed as strong ties, have been rapidly deteriorating – as seen, for example, by several major publicly listed Chinese state-owned enterprises and large private companies withdrawing from the New York Stock Exchange. Will Beijing stand by the Kremlin despite Russia’s invasion of Ukraine? At the same time, Beijing’s close relationship with Moscow and its lack of opposition to Russia’s war in Ukraine have put China in an awkward position. The CCP has realized that cooperation with its long-standing ally and neighbour must come with substantial limits to avoid undermining its own political priorities and interests. Russia’s recklessness may spur Xi and the CCP’s senior leadership to minimize the economic, financial and political risks associated with the Kremlin’s pursuit of war against a country aligned with, and supported by, the West. Will China continue to support its regional partners? On its ties with the Global South, Beijing began to rethink its Belt and Road Initiative (BRI) as the US-led Indo-Pacific strategy gained geopolitical momentum. Regions of Southeast Asia and South Asia received a lot of funding and resources for BRI projects, as seen with Chinese support for the China-Pakistan Economic Corridor. Xi also recently introduced the Global Development and Global Security initiatives, which the political report should flesh out. Beijing is fully aware that it can only prosper if its regional partners prosper Beijing is fully aware that it can only prosper if its regional partners prosper, and it can only achieve resource security and border stability if its southern and western neighbours in Myanmar and Afghanistan cease to fight over land and resources. Chinese foreign policy over the last five years has been a strange combination of high-octane rhetoric and patient pragmatism. To respond to this contradiction, the congress could be used as an opportunity to inject a dose of realism. Read the two further articles in this series: the first is a guide to why the Chinese Communist Party Congress matters; the second discusses the key domestic policy themes that Xi Jinping is expected to outline at this year’s congress. Full Article
ign Director’s Briefing: Assessing foreign policy challenges for the next US president By www.chathamhouse.org Published On :: Tue, 27 Aug 2024 16:37:13 +0000 Director’s Briefing: Assessing foreign policy challenges for the next US president 5 September 2024 — 2:00PM TO 3:00PM Anonymous (not verified) 27 August 2024 Chatham House and Online This briefing will explore what challenges might await the winner of 2024 US presidential election. As the 2024 US Presidential election draws closer, the future direction of American foreign policy seems ever more uncertain. Kamala Harris, the Democratic presidential candidate, appears to be embracing many of Biden’s policies, but she brings a different background, and most likely a different team, so change is likely. Donald Trump has more well-known views on foreign policy, but the context for a second Trump administration would be very different than the first.The next U.S. President will be confronted a world in need of leadership with two major wars, a more assertive and capable China, a climate crisis, ungoverned technological change, emerging powers that demand a seat at the table, and debt distress across much of the developing world.Please join us for this critical conversation covering:How will US-China strategic competition and the threat of conflict over Taiwan challenge US policy makers?What are the risks and challenges posed by Russia’s illegal full-scale invasion of Ukraine?How does war in the Middle East and the threat of regional escalation shape US foreign policy? Full Article
ign Naturally occurring hotspot cancer mutations in G{alpha}13 promote oncogenic signaling [Molecular Bases of Disease] By www.jbc.org Published On :: 2020-12-04T00:06:06-08:00 Heterotrimeric G-proteins are signaling switches broadly divided into four families based on the sequence and functional similarity of their Gα subunits: Gs, Gi/o, Gq/11, and G12/13. Artificial mutations that activate Gα subunits of each of these families have long been known to induce oncogenic transformation in experimental systems. With the advent of next-generation sequencing, activating hotspot mutations in Gs, Gi/o, or Gq/11 proteins have also been identified in patient tumor samples. In contrast, patient tumor-associated G12/13 mutations characterized to date lead to inactivation rather than activation. By using bioinformatic pathway analysis and signaling assays, here we identified cancer-associated hotspot mutations in Arg-200 of Gα13 (encoded by GNA13) as potent activators of oncogenic signaling. First, we found that components of a G12/13-dependent signaling cascade that culminates in activation of the Hippo pathway effectors YAP and TAZ is frequently altered in bladder cancer. Up-regulation of this signaling cascade correlates with increased YAP/TAZ activation transcriptional signatures in this cancer type. Among the G12/13 pathway alterations were mutations in Arg-200 of Gα13, which we validated to promote YAP/TAZ-dependent (TEAD) and MRTF-A/B-dependent (SRE.L) transcriptional activity. We further showed that this mechanism relies on the same RhoGEF-RhoGTPase cascade components that are up-regulated in bladder cancers. Moreover, Gα13 Arg-200 mutants induced oncogenic transformation in vitro as determined by focus formation assays. In summary, our findings on Gα13 mutants establish that naturally occurring hotspot mutations in Gα subunits of any of the four families of heterotrimeric G-proteins are putative cancer drivers. Full Article
ign Hdac3 regulates bone modeling by suppressing osteoclast responsiveness to RANKL [Signal Transduction] By www.jbc.org Published On :: 2020-12-18T00:06:18-08:00 Hdac3 is a lysine deacetylase that removes acetyl groups from histones and additional proteins. Although Hdac3 functions within mesenchymal lineage skeletal cells are defined, little is known about Hdac3 activities in bone-resorbing osteoclasts. In this study we conditionally deleted Hdac3 within Ctsk-expressing cells and examined the effects on bone modeling and osteoclast differentiation in mice. Hdac3 deficiency reduced femur and tibia periosteal circumference and increased cortical periosteal osteoclast number. Trabecular bone was likewise reduced and was accompanied by increased osteoclast number per trabecular bone surface. We previously showed that Hdac3 deacetylates the p65 subunit of the NF-κB transcriptional complex to decrease DNA-binding and transcriptional activity. Hdac3-deficient osteoclasts demonstrate increased K310 NF-κB acetylation and NF-κB transcriptional activity. Hdac3-deficient osteoclast lineage cells were hyper-responsive to RANKL and showed elevated ex vivo osteoclast number and size and enhanced bone resorption in pit formation assays. Osteoclast-directed Hdac3 deficiency decreased cortical and trabecular bone mass parameters, suggesting that Hdac3 regulates coupling of bone resorption and bone formation. We surveyed a panel of osteoclast-derived coupling factors and found that Hdac3 suppression diminished sphingosine-1-phosphate production. Osteoclast-derived sphingosine-1-phosphate acts in paracrine to promote bone mineralization. Mineralization of WT bone marrow stromal cells cultured with conditioned medium from Hdac3-deficient osteoclasts was markedly reduced. Expression of alkaline phosphatase, type 1a1 collagen, and osteocalcin was also suppressed, but no change in Runx2 expression was observed. Our results demonstrate that Hdac3 controls bone modeling by suppressing osteoclast lineage cell responsiveness to RANKL and coupling to bone formation. Full Article
ign Ligand bias in receptor tyrosine kinase signaling [Signal Transduction] By www.jbc.org Published On :: 2020-12-25T00:06:31-08:00 Ligand bias is the ability of ligands to differentially activate certain receptor signaling responses compared with others. It reflects differences in the responses of a receptor to specific ligands and has implications for the development of highly specific therapeutics. Whereas ligand bias has been studied primarily for G protein–coupled receptors (GPCRs), there are also reports of ligand bias for receptor tyrosine kinases (RTKs). However, the understanding of RTK ligand bias is lagging behind the knowledge of GPCR ligand bias. In this review, we highlight how protocols that were developed to study GPCR signaling can be used to identify and quantify RTK ligand bias. We also introduce an operational model that can provide insights into the biophysical basis of RTK activation and ligand bias. Finally, we discuss possible mechanisms underpinning RTK ligand bias. Thus, this review serves as a primer for researchers interested in investigating ligand bias in RTK signaling. Full Article
ign FUS contributes to mTOR-dependent inhibition of translation [Signal Transduction] By www.jbc.org Published On :: 2020-12-25T00:06:31-08:00 The amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD)–linked RNA-binding protein called FUS (fused in sarcoma) has been implicated in several aspects of RNA regulation, including mRNA translation. The mechanism by which FUS affects the translation of polyribosomes has not been established. Here we show that FUS can associate with stalled polyribosomes and that this association is sensitive to mTOR (mammalian target of rapamycin) kinase activity. Specifically, we show that FUS association with polyribosomes is increased by Torin1 treatment or when cells are cultured in nutrient-deficient media, but not when cells are treated with rapamycin, the allosteric inhibitor of mTORC1. Moreover, we report that FUS is necessary for efficient stalling of translation because deficient cells are refractory to the inhibition of mTOR-dependent signaling by Torin1. We also show that ALS-linked FUS mutants R521G and P525L associate abundantly with polyribosomes and decrease global protein synthesis. Importantly, the inhibitory effect on translation by FUS is impaired by mutations that reduce its RNA-binding affinity. These findings demonstrate that FUS is an important RNA-binding protein that mediates translational repression through mTOR-dependent signaling and that ALS-linked FUS mutants can cause a toxic gain of function in the cytoplasm by repressing the translation of mRNA at polyribosomes. Full Article
ign Tyrosine phosphorylation of the scaffold protein IQGAP1 in the MET pathway alters function [Signal Transduction] By www.jbc.org Published On :: 2020-12-25T00:06:30-08:00 IQGAP1 is a key scaffold protein that regulates numerous cellular processes and signaling pathways. Analogous to many other cellular proteins, IQGAP1 undergoes post-translational modifications, including phosphorylation. Nevertheless, very little is known about the specific sites of phosphorylation or the effects on IQGAP1 function. Here, using several approaches, including MS, site-directed mutagenesis, siRNA-mediated gene silencing, and chemical inhibitors, we identified the specific tyrosine residues that are phosphorylated on IQGAP1 and evaluated the effect on function. Tyr-172, Tyr-654, Tyr-855, and Tyr-1510 were phosphorylated on IQGAP1 when phosphotyrosine phosphatase activity was inhibited in cells. IQGAP1 was phosphorylated exclusively on Tyr-1510 under conditions with enhanced MET or c-Src signaling, including in human lung cancer cell lines. This phosphorylation was significantly reduced by chemical inhibitors of MET or c-Src or by siRNA-mediated knockdown of MET. To investigate the biological sequelae of phosphorylation, we generated a nonphosphorylatable IQGAP1 construct by replacing Tyr-1510 with alanine. The ability of hepatocyte growth factor, the ligand for MET, to promote AKT activation and cell migration was significantly greater when IQGAP1-null cells were reconstituted with IQGAP1 Y1510A than when cells were reconstituted with WT IQGAP1. Collectively, our data suggest that phosphorylation of Tyr-1510 of IQGAP1 alters cell function. Because increased MET signaling is implicated in the development and progression of several types of carcinoma, IQGAP1 may be a potential therapeutic target in selected malignancies. Full Article
ign Directors Briefing: Constraints on US foreign policy By www.chathamhouse.org Published On :: Wed, 08 Feb 2023 10:07:13 +0000 Directors Briefing: Constraints on US foreign policy 20 February 2023 — 8:00AM TO 9:15AM Anonymous (not verified) 8 February 2023 Chatham House In conversation with Dr Richard Haass. The US is facing external threats from foreign actors including Russia, China and North Korea. Alongside geopolitical challenges, the US is also experiencing threats from within. Though the US has a long history of enshrining civic rights and democratic freedoms, the institutions of democracy are being weakened through polarization and disinformation. To combat this challenge, the idea of citizenship must be revised and expanded to allow for a functioning, and even a flourishing, democracy. What are the implications of a weakening democracy at home for US foreign policy? How can civic rights in the US be reimagined to reduce divisions within America and protect the future of democracy? Full Article
ign Time-resolved Mass Spectrometry of Tyrosine Phosphorylation Sites in the Epidermal Growth Factor Receptor Signaling Network Reveals Dynamic Modules By www.mcponline.org Published On :: 2005-09-01 Yi ZhangSep 1, 2005; 4:1240-1250Research Full Article
ign Quantitative Phosphoproteomics of Early Elicitor Signaling in Arabidopsis By www.mcponline.org Published On :: 2007-07-01 Joris J. BenschopJul 1, 2007; 6:1198-1214Research Full Article
ign Quantitative Phosphoproteomics Applied to the Yeast Pheromone Signaling Pathway By www.mcponline.org Published On :: 2005-03-01 Albrecht GruhlerMar 1, 2005; 4:310-327Research Full Article
ign The Democratic Republic of the Congo’s foreign policy priorities By www.chathamhouse.org Published On :: Fri, 18 Oct 2024 19:42:13 +0000 The Democratic Republic of the Congo’s foreign policy priorities 29 October 2024 — 1:00PM TO 2:00PM Anonymous (not verified) 18 October 2024 Chatham House and Online The DRC’s Minister of Foreign Affairs discusses the country’s approach to regional stability and the role of international partnerships in securing economic prosperity. At this event, HE Thérèse Kayikwamba Wagner, Minister of Foreign Affairs of the DRC, will discuss DRC’s regional diplomatic priorities and international agency, including its ambitions around global critical mineral supply chains. The Democratic Republic of the Congo (DRC) faces foreign policy tests as it manages a complex security crisis and regional tensions, alongside global economic and trade partnerships.Entrenched conflict in eastern DRC continues to have wider regional implications. Troops from the Southern African Development Community (SADC) deployment are tackling a growing mandate as the UN’s peacekeeping mission prepares to withdraw. Tensions with Rwanda also remain high despite mediation efforts under the Luanda process.As a major producer of critical minerals for the global energy transition, the DRC seeks to benefit from growing international demand without sacrificing value addition. New and existing frameworks with international partners will remain key to this vision, including for the development of regional infrastructure networks such as the Lobito Corridor.At this event, HE Thérèse Kayikwamba Wagner, Minister of Foreign Affairs of the DRC, will discuss the DRC’s regional diplomacy and international agency, including the country’s positioning in global critical mineral supply chains.Please note that in-person attendance for this event will be balloted. Guests looking to join in-person must register interest in joining. Full confirmations to successful applicants will be sent on Friday 25 October. Full Article
ign Independent Thinking: Will debt constrain Western foreign policy? By www.chathamhouse.org Published On :: Thu, 24 Oct 2024 15:15:59 +0000 Independent Thinking: Will debt constrain Western foreign policy? Audio john.pollock 24 October 2024 Patrick Wintour and Ranil Dissanayake join the podcast ahead of the UK Budget to discuss the high debt facing G7 economies and its impact on foreign, defence and development policies. On this episodeAhead of a crucial Budget by Chancellor Rachel Reeves, UK national debt is at almost 100 per cent of GDP – and it’s not alone. Many G7 economies face massive debt levels, restricting ambitions when it comes to foreign policy and global engagement. Bronwen Maddox is joined by Patrick Wintour, the Guardian’s diplomatic editor and Ranil Dissanayake, a senior research fellow at the Centre for Global Development. With them are Olivia O’Sullivan and David Lubin from Chatham House.About Independent ThinkingIndependent Thinking is a weekly international affairs podcast hosted by our director Bronwen Maddox, in conversation with leading policymakers, journalists, and Chatham House experts providing insight on the latest international issues.More ways to listen: Apple Podcasts, Spotify. Full Article
ign The break-up of Scholz’s coalition government signals the end of Germany’s old economic model By www.chathamhouse.org Published On :: Tue, 12 Nov 2024 15:12:46 +0000 The break-up of Scholz’s coalition government signals the end of Germany’s old economic model Expert comment jon.wallace 12 November 2024 The coalition could not agree how to fund new support for Ukraine and failed to fully implement the ‘Zeitenwende’. A new government must push through reform. As Europeans were still processing Donald Trump’s victory in the 2024 US presidential election, an acrimonious break up occurred 4000 miles east of Washington DC.Reports had been circulating for weeks about the fragile state of Germany’s ‘traffic light’ coalition government led by German Chancellor Olaf Scholz, consisting of the centre-left Social Democratic Party (SPD), the Green Party, and liberal Free Democratic Party (FDP).The expectation had been that the coalition would hold on for a few more weeks and might even be given a new lease of life by Trump’s re-election. Instead, it collapsed on the day Trump’s win was confirmed. An unusually angry Scholtz announced in a live address that he had fired FDP Finance Minister Christian Lindner, effectively breaking up the coalition. Related content Independent Thinking: Can Germany lead in a divided Europe? At the heart of the dispute was the so-called ‘debt brake’ – a constitutional mechanism which restricts Germany’s annual public deficit to 0.35 per cent of GDP. Lindner proposed a set of reforms which were unpalatable to the SPD and the Greens. In response, Scholz suggested declaring an emergency, which would have suspended the debt brake. That in turn was unacceptable to Lindner, leading to his sacking by the Chancellor.Practically, this means the SPD and the Greens are now in a minority coalition, without agreement on the 2025 budget or the votes in parliament to pass it. They also still face the challenge of the debt brake.A vote of confidence will take place in December, with elections to be held before the end of February 2025 latest.The end of Germany’s economic modelAt the root of Germany’s political crisis is the country’s economic model. For decades, Germany relied on a system that depended on cheap Russian gas, cheap imports of consumer goods from China, high-value exports – particularly in the automotive sector – and the US security umbrella.With Russian energy no longer viable, the global economic landscape shifting, and Donald Trump on his way back to the White House, that model is no longer workable. And Germany’s economy is expected to contract by 0.2 per cent in 2024 – a contraction for the second year running. Germany has struggled to turn around its economic woes, with the car industry particularly affected. The ‘Zeitenwende’, announced by Scholz in the wake of Russia’s full-scale invasion of Ukraine, should have signalled a turnaround of both foreign and economic policy, given how much the two are interconnected. Yet on both fronts, too little changed.Germany’s reliance on Russian gas did come to an abrupt end in 2022. And Germany is Ukraine’s second largest military aid donor after the US, while accepting the most Ukrainian refugees.But the ‘Zeitenwende’ turnaround ended there. Scholz’s coalition government failed to prepare for long-term investment in defence at the levels required by creating an off-budget defence spending fund which would have run out in 2027. The draft budget for 2025 showed defence spending would have been cut, as would support for Ukraine.Germany has also struggled to turn around its economic woes, with the car industry particularly affected. Cheap Chinese EVs and new energy technologies are competing with Germany’s most powerful companies. Volkswagen, the country’s largest car manufacturer, has announced plant closures and layoffs due to shrinking profit margins. To the west, Trump’s threat to impose 10 to 20 per cent tariffs on all EU imports meant share prices of Volkswagen, BMW, Mercedez-Benz and Porsche all dropped between 4 to 7 per cent following news of his re-election.To the east, trade tensions between the EU and China are intensifying. Yet rather than choosing to diversify, German companies have doubled down on their bets in China, with German investment in the country rising from €6.5bn for the whole of 2023 to €7.3bn in the first half of 2024 alone – only exposing carmakers further.Germany’s support for UkraineLike French President Emmanuel Macron, Scholz had already been weakened by the results of the European Parliamentary elections in June. With the collapse of his traffic light coalition, the EU’s Franco-German ‘engine’ is now well and truly stalled – until new leadership can be found. This weakness comes at a perilous moment when clear, united European leadership, and much increased funding, is needed to shore up support for Ukraine. Full Article
ign Undercurrents: Episode 12 - Trump's Visit to the UK, and Japanese Foreign Policy in Asia By f1.media.brightcove.com Published On :: Thu, 12 Jul 2018 00:00:00 +0100 Full Article
ign Undercurrents: Episode 14 - Sustainable Energy for Refugees and Australian Foreign Policy By f1.media.brightcove.com Published On :: Fri, 10 Aug 2018 00:00:00 +0100 Full Article
ign The Failed Marshall Plan: Learning from US Foreign Policy Missteps By f1.media.brightcove.com Published On :: Mon, 10 Sep 2018 00:00:00 +0100 Full Article
ign Iran’s New Foreign Policy Challenges By f1.media.brightcove.com Published On :: Tue, 06 Nov 2018 00:00:00 +0000 Full Article
ign Undercurrents: Episode 25 - The End of Liberal Foreign Policy, and the Legacy of the Paris Peace Conference By f1.media.brightcove.com Published On :: Thu, 31 Jan 2019 00:00:00 +0000 Full Article
ign A New Vision for American Foreign Policy By f1.media.brightcove.com Published On :: Mon, 21 Oct 2019 00:00:00 +0100 Full Article