Escherichia coli DNA Pol I can carry out three enzymatic reactions: It possesses 5' -> 3' DNA polymerase activity and 3' -> 5' and 5' -> 3' exonuclease activity. Pol I can be cleaved by mild treatment with subtilisin into two fragments; the larger fragment is known as the Klenow fragment. The Klenow fragment retains the polymerizing activity and the 3' -> 5' exonuclease of the holo-enzyme but lacks its powerful 5' -> 3' exonuclease activity. These enzymes and their applications in molecular cloning are introduced here.

The Klenow fragment, which retains the template-dependent deoxynucleotide polymerizing activity and the 3' -> 5' exonuclease of the holo-enzyme but lacks its powerful 5' -> 3' exonuclease activity, is used to fill recessed 3' termini of dsDNA. In this protocol, fragments suitable as templates for the end-filling reaction are produced by digestion of DNA with an appropriate restriction enzyme. The Klenow enzyme is then used to catalyze the attachment of dNTPs to the recessed 3'-hydroxyl groups.

BACKGROUND AND PURPOSE:

The use of invasive cerebral angiography with CTA for active treatment of patients with suspected ischemic strokes has been increasing recently. This study aimed to identify the incidence of postcontrast acute kidney injury using baseline renal function when CTA and cerebral angiography were performed sequentially.

Thị trường trái phiếu doanh nghiệp sôi động với sức nóng tăng lên từ cả phía cung và phía cầu. Nhà đầu tư trong nước mua tới 22.664 tỷ đồng (61,5%) trái phiếu doanh nghiệp BĐS.

Due to the lack of regulation and sanctions, home buyers has to bear higher risk from property deposits, especially for land with land with high value.

Một nghiên cứu mới đây cho thấy chi phí trang hoàng văn phòng mới tại Hồng Kông đang xếp sau 6 thành phố lớn ở Nhật và Úc. Điều này cũng phần nào an ủi những người đi thuê nhà tại nơi mà suốt 4 năm liền vẫn luôn đứng đầu bảng xếp hạng giá thuê thế giới.

A cheap, lightweight smartphone-heated device can test for DNA in blood, urine and other samples in a fraction of the time it takes to test in a lab

SAVANNAH, Georgia—Allegations Friday that a Georgia district attorney blocked police officers from arresting the killers of 25-year-old Ahmaud Arbery were just the latest blow to a local law enforcement apparatus that has been rocked by a series of troubling and deadly scandals.

Brunswick DA Jackie Johnson’s office allegedly prevented the Glynn County Police Department from arresting Travis and Gregory McMichael in connection with the shooting death of Arbery, an unarmed black man, in late February, the Atlanta Journal-Constitution reported. Johnson has recused herself from that case. But one Glynn County commissioner suggested she personally intervened in early plans to make arrests “to protect her friend” Gregory McMichael. McMichael was a former cop and investigator in the Brunswick DA’s office for 25 years who had reportedly investigated Arbery in the past. 

It is far from the first time the office—and local law enforcement more generally—had come under scathing scrutiny. 

Read more at The Daily Beast.

Saudi Aramco’s IPO saga has turned into “A Tale of Two Princes.” As Saudi Arabia’s day-to-day ruler Crown Prince Mohammed bin Salman has guided the IPO far from public view, his half-brother Prince Abdulaziz bin Salman stepped onto center stage in Vienna last week, presiding over a crucial gathering of OPEC and its oil-producing allies […]

The FAA’s app—which tells you where you can and can’t fly your drone—ignores both local and national regulations

Janelle Shane talks about the absurdity, perils, and limits of AI

From haute gardening hats, to nettles dresses and sexy necklines, here are the trends that are coming for you for this spring/summer

• Read more from the spring/summer 2020 edition of The Fashion, our biannual style supplement

Afraid of looking like a dunce when it comes to your fashion knowledge? We’ve created a cheat sheet for the new season.

Trump administration whistleblower Rick Bright teared up while ripping the Trump's response to the coronavirus: "We could've done something and we didn't."

Coffee has been linked to changes on our DNA that affect how active certain genes are. The finding may help explain some of coffee's touted health benefits

Sania Mirza took to social media to post a picture with her son Izhaan.

Joe Biden's campaign has signed a joint fundraising agreement with the DNC that will help Democrats close the gap with President Donald Trump's massive war chest.

A roundup of some of the most popular but completely untrue stories and visuals of the week. None of these are legit, even though they were shared widely on social media. The Associated Press checked them out. Here are the facts:

___

CLAIM: Photo shows Joe Biden posing with former ...

Arbery, who would have turned 26 on Friday, was shot to death after being chased by two men who thought he was a burglar.

This will be my 23rd Mother’s Day without my mother and while it gets easier, it is never easy.

Donald Trump has instructed his administration to temporarily halt funding to the World Health Organisation over its handling of the coronavirus pandemic.

A former NHS nurse who returned to the frontlines to help fight the coronavirus pandemic has shared the moment she held an elderly woman's hand during her last breaths so she didn't die alone from the virus.

A north London teenager, 16, has been found guilty of kidnapping a stranger in the street and forcing him to withdraw money from a cash machine.

You can donate at virginmoneygiving.com/fund/FoodforLondonNOW

The top stories you're waking up to Listen to your Morning Bulletin on Google Assistant and Amazon Alexa smart speakers from 7am every weekday

Garden centres in England will be allowed to reopen next week as part of the Government's first-step measures to ease the coronavirus lockdown.

There are decisions being made right now that could have an effect on global populations for generations to come. As part of this project, we commissioned an artist to investigate some of the themes raised in the podcasts. This work of fiction imagines a future where gene editing has become mainstream and discusses the moral, ethical and political divides that this might create

Scientists have created glowing flowers after editing the DNA of a plant.

Earlier Matt Frei spoke to Mark Roscrow, the Chair of Trustees for the Health Care Supplies Association

Elie Mystal

The post We Didn’t Need Video to Know Ahmaud Arbery Was Lynched appeared first on The Nation.

"The fact that we are the world’s largest democracy and we are all aware that power and a role in global affairs for a nation comes from economic strength."

No spoilers here, but details about character relationships, fates are out there.

Another 'Star Wars' actor has added their voice to the criticism of Rian Johnson's movie.

Professional poker player Phil Galfond overcame a €900,000 deficit in a 25,000-hand head-to-head challenge against a pot-limit Omaha regular known as "VeniVidi1993" online.

A St. John’s genetics specialist has found DNA connections that link the long-vanished Beothuk people to contemporary people, almost two centuries after the last known Beothuk died. 

He called Exotic 'unbelievably cruel'

'This wasn't the most joyous season for me,' actor admitted in candid new interview

'Man, I was wrong'

Actor was ridiculed for remarks as people pointed out they were unlikely to forget current predicament anytime soon

All the clues you need in one picture

The first season ended with a cliffhanger – did Jen kill Steve or not? But what is most poignant about the second season is not who killed him, but how well the show deals with grief, writes Charlotte Cripps

• State auditor says Favre will repay money for no-show speeches
• Hall of Fame quarterback faces no criminal charges after report

Former NFL quarterback Brett Favre is repaying $1.1m in welfare money that he received for multiple speeches where he did not show up, the Mississippi state auditor said Wednesday.

Auditor Shad White said his office received $500,000 from Favre on Wednesday, plus a commitment that Favre will repay the other $600,000 in installments over the next few months.

Related: NFL tells teams to have facility reopening protocols in place by next week

NSW Health assessment the cruise ship was ‘low risk’ introduced ‘an unfortunate element’ into his decision-making

• Sign up for Guardian Australia’s daily coronavirus email
• Download the free Guardian app to get the most important news notifications

A New South Wales harbour master has told an inquiry he “did not understand” an email that told him to treat the Ruby Princess “as if it has a positive Covid-19 result” when it came into Sydney Harbour on 18 and 19 March.

Cameron Butchart, who was the duty harbour master on 18 March, said NSW Health’s assessment that the ship was “low risk” introduced “an unfortunate element” into his decision-making.

With more Australian artists being asked to play for free in the lockdown, many are asking if it might do more harm than good

If live music died in mid March, it’s sure been noisy at the funeral. On platforms old and new, live gigs performed at home have streamed from trickle to tidal wave, breaking over the mobile devices of captive audiences. Global gig guide aggregator Bands In Town has added a livestream dropdown, and a new Australian state has been ceded by Eventfinda and tucked alphabetically between Victoria and Western Australia: the state of “Virtual”.

For fans it’s been fun. We’re loving seeing musicians’ pets and plants and enormous fingers fumbling for the flip screen button and, unless we’ve bought a URL ticket, there’s scandalously little to lose by dropping into, and out of, a show.

The cardinal maintains he didn’t know about the Victorian town’s notorious paedophile priests, a claim the royal commission found ‘implausible’

“Why isn’t all of Australia talking about what happened here in Ballarat?”

That’s the question Clare Linane remembers asking her husband, Peter Blenkiron, 12 years ago as they were sitting in the kitchen talking about his abuse. Linane’s husband, brother and cousin had all been abused when they were children between 1973 and 1974 by Christian Brother and now convicted paedophile Edward “Ted” Dowlan. They knew they were among thousands of people living in and around Ballarat – Victoria’s largest inland city – who had been affected by child sexual abuse perpetrated by clergy.

Hesmondhalgh said she did a "decent job" of playing Corrie's first trans character - but would not take the part now

The comedian asked her fans to 'please ignore' the lengthy post which has been doing the rounds on social media

The new Twilight novel, told from Edward's perspective, will be released on August 1

The new Twilight novel, told from Edward's perspective, will be released on August 4

Strictly’s newest judge learned to stick up for herself in apartheid-era South Africa. She talks racism, the ‘rift’ with her sister Oti – and learning to dance amid knife fights

Motsi Mabuse is remembering the rough dance halls of apartheid-era South Africa and the shocking sights she would see as she took her first tentative steps across their floors. “When we had competitions,” she says, “we didn’t have security and people would be drunk and starting fights. We were just kids and we’d watch people with knives running through while we were in the middle of a routine. Compared to that, Strictly isn’t so difficult.”

Mabuse, the newest judge on Strictly Come Dancing, first fell for the glitterball world at the age of nine after watching couples waltz, swing and cha-cha-cha while on holiday in Durban. “What I love about my parents is that they didn’t say: ‘Oh, you can’t do that.’ They found a way. But we had a lot of backlash, being the only black kids. People would laugh at us and call us names. We were bullied, but we just kept on coming back – and then we beat them.”