How do you select one image to represent half a century of photography and art? You don’t

Transform your Zoom virtual background or computer desktop into a work of art

Graffiti artists and muralists are sending messages of hope and despair with coronavirus public art

“Care Package” showcases Asian American and Pacific Islander artists, writers and scholars as sources of solace during the Covid-19 pandemic

These paintings, sculptures and illustrations honor the bonds of motherhood

The projects are helping Australia's drought-stricken rural towns find new life as outdoor art galleries

It all began when three frustrated women sought the no-bounce zone

The first major exhibition of its kind, "Hearts of Our People," boasts 82 pieces from 115 Native women across North America

In the late 1300s, tea was introduced to Japan from mainland China, transported in delicate jars. Over the years, as drinking tea became a prized activity in Japan, so too did the jars in which it was stored

Hadrian’s Wall was a 73 mile barrier stretching from coast to coast, splitting the warlike north of Britain from the more docile south. It was the Roman Empire’s way of imposing peace in a hostile land

Highway 8, otherwise known as the Dempster Highway, will be closed to all traffic at 5 p.m. on Friday, according to the government of the Northwest Territories.

As New Brunswick's economy slowly opens up, the province's daycares are waiting for the call to action, says the head of the group that represents them. 

It looks like New Brunswickers will see more snow Saturday with a snowfall warning issued for central and northern parts of the province. 

Some farmers across the province are worried about getting their fuel in time for spring seeding. The Agricultural Producers Association of Saskatchewan says it has been fielding complaints this week about delays at the Co-op cardlock near Moose Jaw.

A St. John's cardiologist says public health restrictions put in place under the COVID-19 pandemic, while justified, may be harming cardiac patients whose procedures have been postponed indefinitely as well as heart patients who are avoiding hospitals.

The vessel, formerly known as the Edmonton Queen, got skewered by its own moorings last month as the icy North Saskatchewan River began to thaw.

The following is a guest post by Martha H. Kennedy, Curator of Popular & Applied Graphic Art, Prints & Photographs Division. During Will Eisner Week, March 1-7, 2020, the Library of Congress joins art schools, libraries, universities, and museums in a global celebration of this legendary creator’s contributions to the world of comic art and […]

The following is a guest post by Katherine Blood, Curator of Fine Prints, Prints & Photographs Division. As the Library of Congress marks its 220th year of serving the nation, the publication of a new guide tells two stories: how staff have for decades worked with art professionals to build the collections and how by […]

The following is a post by Kristi Finefield, Reference Specialist in the Prints & Photographs Division, and member of the Picture This blog team. As the Library of Congress marks its 220th year, we take the opportunity to explore one example of its efforts to sustain and celebrate the arts in its physical spaces. Above […]

Successful modification of the bacterial artificial chromosome (BAC) after two-step BAC engineering is confirmed in two separate polymerase chain reactions (PCRs). The first reaction (5' co-integrate PCR) uses a forward 5' co-integrate primer (a sequence located upstream of the 5' end of the A-box) and a reverse 3' primer on the vector (175PA+50AT) or within the reporter sequence or mutated region as appropriate. The second reaction (3' co-integrate PCR) uses a forward 5' primer on the recA gene (RecA1300S) and a reverse 3' co-integrate primer (a sequence located downstream from the 3' end of the B-box). Those colonies shown to be positive in PCR analysis are further tested for sensitivity to UV light. After the resolution, colonies that have lost the excised recombination vector including sacB and recA genes become UV light sensitive.

Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.

Plasmid DNA is prepared from the recombinant shuttle vector pLD53.SCAB/A-B created by cloning of the A and B homology arms for two-step bacterial artificial chromosome (BAC) engineering. To confirm that the A-box and B-box arms have been successfully incorporated into pLD53.SCAB, the pattern of enzyme digestion of the modified plasmid is compared with that of the unmodified pLD53.SCAB. Once the shuttle vector is shown to carry the proper sequences, it is ready for transfer into the BAC host.

This protocol describes the preparation of the shuttle vector before its introduction into bacterial artificial chromosome (BAC) host cells for BAC two-step engineering. The homology arm sequences, prepared previously, are introduced by ligation into the digested shuttle vector DNA to provide sites for recombination within the BAC clone. Crude lysates of individual bacterial transformants serve as templates in polymerase chain reaction (PCR) analysis to confirm the presence of the homology arms in the recombinant shuttle vector.

The 700-bp A homology arm (A-box) and the 700-bp B homology arm (B-box) are amplified by polymerase chain reaction (PCR) using purified bacterial artificial chromosome (BAC) DNA as template for two-step BAC engineering. The resulting A-box PCR product contains an AscI site at its 5' end (the 5' primer incorporates an AscI site, and the 3' primer does not incorporate any restriction sites). The B-box PCR product contains an XmaI site at its 3' end (the 5' primer does not incorporate any restriction sites, and the 3' primer incorporates an XmaI site). The amplification products are then digested with the appropriate restriction endonucleases to render them suitable for cloning into the shuttle vector.

In two-step bacterial artificial chromosome (BAC) engineering, a single plasmid is introduced into the BAC-carrying cell lines. The shuttle vector pLD53.SCAB (or pLD53.SCAEB) carries the recA gene and the R6K origin, which requires the protein to replicate. PIR2 cells, expressing , are typically used for the amplification of the vector and maintain about 15 copies/cell of the donor vector, which is relatively stable in this host.

BIS Press Release - Easing trade tensions lift sentiment: BIS Quarterly Review, 8 December 2019

BIS Press Release - Shaping the future of payments: BIS Quarterly Review, 1 March 2020

We can expect to see more seasonable temperatures by next weekend, says a meteorologist from Environment Canada.

Winnipeg will soon test "small cell" technology to improve cellular reception in parts of Winnipeg, ahead of a possible future transition to 5G service. 

They're not necessarily treating sick patients in hospitals, but a number of Manitoba-based scientists are working long hours and facing incredible pressure to battle the novel coronavirus from their labs and research facilities.

Artists from Michigan and around the world are painting 50 murals in Flint to refocus the city's image on art rather than the lead-tainted water crisis.

All remaining criminal charges of city and state officials stemming from the Flint Water Crisis have been dismissed.

Vincent Novara, Head of the Music Division's Acquisitions & Processing Section, describes his section's new teleworking practices during a global pandemic.

The following is a guest post from Music Reference Specialist James Wintle. Let me begin with a personal anecdote. My parents are or were both musicians – my father was a composer – and so my appreciation for classical music was probably equal parts nature and nurture. So, when I entered graduate school as a […]

11 startups to pitch at NEXT Canada's virtual Venture Reveal  BetaKit

From stage to sofa: NAC brings local arts to homes in new livestream initiative  Connected to India

Melissa Tancredi and Rhian Wilkinson were pillars of the Team Canada for nearly two decades. Together, they've lived the highs and lows of the women's national soccer program - from the awe of their first Games in Beijing 2008, the heartbreak and redemption in London 2012, and finally, solidifying Canada's reputation as a soccer world power in Rio 2016. It's been a rollercoaster they'd ride over and over again.

Last month, the National Arts Centre launched #CanadaPerforms, an initiative that pays musicians and writers to perform from their living rooms. Poet Zoey Roy gave an intimate performance from her living room in Kingston. 

Mayor Jane Castor has issued a letter of apology after Tampa didn't exactly give the best of welcomes to one of its newest and most famous residents when NFL superstar Tom Brady was ejected from a downtown park while working out.

New England head coach Bill Belichick said not taking a quarterback in the Patriots' first post-Tom Brady draft "wasn't by design."

There's no question that COVID-19 has uprooted the lives of people across Metro Vancouver and around the world. Data can be one way to show how, exactly, the pandemic has changed the way we move and operate in our daily lives.  

This summer nearly 1,000 Roma children in Roma villages throughout Central and Eastern Europe heard the Gospel.

After 10 years in North Africa, an OM worker sees a change in the hearts of neighbours and friends.

Their desire to help leads a Love Moldova outreach team to a woman whose loss of her toes leads to the salvation of her soul.

Toronto officials reported 180 new cases of COVID-19 on Thursday, a figure that represents a dip when compared to the more than 200 cases announced daily this week. 

As automakers look to restart the industry, union representatives are looking to ensure safety is the number one priority for workers.

A young fan of the Jurassic Park film franchise sporting a Tyrannosaurus Rex costume got to meet some of the cast and crew who have been filming the latest instalment of the franchise in his hometown of Merritt, B.C.

As truckers move vital supplies such as food, hand sanitizer and ventilators during the COVID-19 pandemic, they're pleading with governments to allow more truck stops to reopen so they can get meals, showers and rest. Social media groups and individual businesses are doing their part to thank truckers.

A man in Kamloops, B.C., has died and two others are in hospital with life-threatening injuries after a stabbing at a party at an apartment complex Saturday night.