The pilum, a vital weapon in Rome’s imperial ambitions, was a simple iron spear fixed with two bolts onto a heavy wooden post. It was remarkably effective against an attacker charging at high speed.

Shadunjen van Kampen, a 21-year-old member of the Champagne and Aishihik First Nations in Yukon, recently completed her written exams and flight test. She plans to be a commercial bush pilot. 

The Heritage neighbourhood's free little libraries are being transformed.

Some farmers across the province are worried about getting their fuel in time for spring seeding. The Agricultural Producers Association of Saskatchewan says it has been fielding complaints this week about delays at the Co-op cardlock near Moose Jaw.

A physiotherapist in Thunder Bay, Ont., has had his licence suspended until September, as part of a disciplinary hearing.

The tourism and hospitality industries, which play a large role in the economy of almost every community in northwestern Ontario, are taking a beating during the COVID-19 pandemic. Now, the manager of Tourism Thunder Bay is figuring out how “dire” the situation is, and what could be done to help.

Provincial Police have a suspect in custody after a firearms incident Thursday morning at the hospital in the small northwestern Ontario community of Rainy River.

Parents-to-be who will have their new child delivered at the Thunder Bay Regional Health Sciences Centre have to follow a different set of guidelines than found at many other hospitals across Ontario.

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Crombie REIT, which owns the Avalon Mall, is awaiting details before applying for government's commercial rent relief.

From age 17, Ana Maria prayed to serve God in Switzerland. While she waited, she became a dance instructor with no idea dance would become her ministry.

When Elizabeth May, parliamentary leader of the federal Green Party, proclaimed on Tuesday that "oil is dead," she was correct in a philosophical sense. But not in a practical, real world sense.

Police are investigating a report of a suspicious package in north Edmonton.

The following is a guest post by Hanna Soltys, Reference Librarian, Prints & Photographs Division. The post was written with the help of Sara W. Duke, Curator of Popular and Applied Graphic Art.   While professional baseball’s Opening Day will take place at a later date, the spirit and excitement of the day still live […]

The only way to solubilize many antigens for immunoprecipitation is by denaturation. This cell lysis protocol is ideally suited for this purpose to release proteins from complex structures or reveal antibody epitopes hidden within native proteins. Short linear epitopes may not be accessible to antibodies within the native tertiary and quaternary protein structures, but they become exposed upon the unraveling of proteins, exposing their secondary structure. Antibodies otherwise not suitable for the immunoprecipitation of proteins prepared under nondenaturing conditions are now able to bind these antigens of interest in cell lysates prepared under denaturing conditions. These antibodies may also work well for immunoblotting purposes when the protein target is completely denatured. Harvested cells in this protocol are washed in tris-buffered saline (TBS) before lysis in 2% sodium dodecyl sulfate (SDS)-containing Lysis buffer for 10 min at 100°C. The resulting sample is diluted 20-fold in TBS to reduce the SDS concentration to ≤0.1% before the addition of an antibody for immunoprecipitation. Addition of 2% bovine serum albumin (BSA) or 0.1% Nonidet P-40 to the TBS before an immunoprecipitation, respectively, ensures either removal of SDS from the target protein or retaining denatured proteins in solution.

Differential detergent fractionation of cells is a rapid method for extraction of cytoplasmic and nuclear proteins in preparation of an immunoprecipitation. This method can be applied for use of adherent or suspension cells and can significantly reduce nonspecific background in an immunoprecipitation by separation of cellular compartments into individual fractions. The lysis of cells by differential detergents permits the rapid extraction of proteins from the cytoplasm (digitonin), the cytoplasmic membranes, and organelles (Triton X-100), and nucleoplasm (Tween/DOC), facilitated through the use of distinct extraction buffers. Cytoplasmic and nuclear matrix proteins as well as DNA are left behind during the detergent-based extraction.

Purified RNA may need to be concentrated by precipitation for downstream applications. Precipitation of RNA with ethanol (or isopropanol) is the standard method to recover RNA from aqueous solutions.

For most immunoblots developed with chemiluminescence or with fluorochrome-based detection systems, it is possible to remove the primary and secondary antibodies from the membrane without affecting the bound antigen. This allows you to reuse the membrane for detection of another protein antigen. The blots developed with chromogenic substrates can also be stripped of antibodies and reprobed, but the bands detected in the first round of immunoblotting will remain unaffected. Stripping and reprobing of the membrane are particularly useful when the amount of sample is limited or when it is important to accurately compare the signal between two different protein antigens in the same sample. Examples of such experiments include determining the levels of a protein antigen in a series of samples relative to the loading control and comparison of the phosphorylated form to the total levels of the protein in the sample.

There are many uses for antibodies labeled with metal ions. Most of these methods involve first attaching a metal chelator to the antibody molecule. This is achieved using standard cross-linking chemistry and then adding the desired metal at appropriate concentration and pH. The method described here outlines a basic procedure for creating a lanthanide conjugate. Lanthanide conjugates are used for proximity assays, as MRI contrast agents, or for mass cytometry experiments. Different metals and chelators can be substituted, but the basic procedures are similar.

We assess the dynamics of volatility spillovers among global systemically important banks (G-SIBs). We measure spillovers using vector-autoregressive models of range volatility of the equity prices of G-SIBs, together with machine learning methods. We then compare the size of these spillovers with the degree of systemic importance measured by the Basel Committee on Banking Supervision's G-SIB bucket designations.

No global real estate market despite higher price synchronisation and growing role of international investors, central banks find (Press release, 18 February 2020)

BIS Press Release - Shaping the future of payments: BIS Quarterly Review, 1 March 2020

When we find something particularly beautiful or impressive, we literally get big eyes: Our pupils dilate. The pupil controls how much light enters the eye and falls on the retina.

Graduation is on hold for Dalhousie University medical student Manveer Bal, so he's decided to spend his newfound free time volunteering at the epicentre of the COVID-19 outbreak in Nova Scotia.

As Nova Scotians gazed up at the Canadian Forces Snowbirds soaring through the clouds last weekend, Matt MacKenzie was looking down and feeling the weight of successive tragedies that had enveloped his home province. 

Cleveland Indians reliever Emmanuel Clase was suspended 80 games by Major League Baseball on Friday for testing positive for a banned performance-enhancing drug.

Matt Keough, the former Oakland Athletics pitcher and special assistant, has died. He was 64. He was an American League all-star as a rookie in 1978 and two years later comeback player of the year.

They're not necessarily treating sick patients in hospitals, but a number of Manitoba-based scientists are working long hours and facing incredible pressure to battle the novel coronavirus from their labs and research facilities.

Merchants soon will be able to sell products on Google Shopping at no charge. Previously, they had to pay per click, but the cost was not fixed. There was no minimum, but they had to set a maximum for ad spend and Google would stop displaying their ads once the maximum was reached. Starting next week, search results on the Google Shopping tab will consist primarily of free product listings.

Consumers and companies worldwide have ramped up online ordering for software products and digital goods as they struggle to improve productivity and security while working remotely and spending more time at home. The sharp spike in online commerce aligns with the timing of the current global pandemic. Software-based offerings accounted for the highest levels of growth.

Shopify has unveiled an app that lets users discover local businesses, receive relevant product recommendations from their favorite brands, check out effortlessly, and track all their online orders. It can gather and track orders automatically, but it also works without auto-tracking. Consumers can get a customized feed with deals, trending items and recommendations from their favorite stores.

Michigan officials are ordering a Flint hospital to take steps to reduce the risk of exposure to Legionella bacteria and Legionnaires' disease at the facility.

OK, you’re gonna hear a lot about Joementum.

Commentary on Red Book statistics: Shaping the future of payments, November 2019

Speech by Mr Benjamin E Diokno, Governor of Bangko Sentral ng Pilipinas (BSP, the central bank of the Philippines), at an investor call with Standard Chartered Bank, Manila, 22 April 2020.

Ice dancers Piper Gilles and Paul Poirier headline the Canadian team that will compete at this year's world figure skating championships, but Skate Canada is waiting until next month to make a final decision on several of their teammates.

For Canadian champion Roman Sadovsky and former Canadian champions Nam Nguyen and Keegan Messing, the Four Continents results will determine who takes the one spot available in the men's event at the worlds.

Canada's Joseph Phan was eighth after a highly competitive men's short program Wednesday at the world junior figure skating championships.

Quebec Health Minister Danielle McCann says the government is evaluating whether to allow the world figure skating championships to go ahead next week in Montreal.

The world figure skating championships, scheduled for March 18-22 in Montreal, have been cancelled because of the spread of COVID-19.

With this year's worlds cancelled because of the COVID-19 pandemic, Rob Pizzo looks at the last time the event didn't happen.

As the planet deals with the pandemic and the crisis involving the spread of COVID-19, the world of sport watches and waits alongside the global population. This Saturday, March 21, will be the first of five weeks of programming at CBC Sports which will showcase some of the memorable moments from the most recent editions of the Olympics.

Watch Olympic Games Replay, a look back at some of the most exciting moments in past Games. On this week's edition of the program, watch action from the Pyeongchang 2018 figure skating competitions.

The Canadian four-time world champion figure skater hammed it up along with fellow Canadian skater Kaitlyn Weaver, to raise funds for the United Nation's COVID-19 Solidarity Response Fund.

The World pairs champion and Olympic gold medallist played the piano during a special hosted by fellow Canadian figure skater Kaitlyn Weaver, to raise funds for the United Nation's COVID-19 Solidarity Response Fund.

Former Canadian figure skater and Olympic medallist Joannie Rochette will be working at Quebec's long-term care homes hit hard by COVID-19.

The following is a guest post from Music Reference Specialist James Wintle. Let me begin with a personal anecdote. My parents are or were both musicians – my father was a composer – and so my appreciation for classical music was probably equal parts nature and nurture. So, when I entered graduate school as a […]