The shares have been priced at ₹289, the upper limit of its price range

An analysis of buyer trends of this year’s Amazon Great Indian Festival sale so far has revealed an affinity of Bengal-based buyers towards renewable and artificial intelligence appliances like solar panels, single-equipment home gyms, robotic vacuum cleaners

The Kolkata Traffic Police registered cases against 841 people on charges such as rash driving and drunken driving. In an incident in Ward 110 of Patuli area, an adolescent boy was injured in a crude bomb explosion. In Howrah district, three people, including two children, died after a house where firecrackers had been stored caught fire.

Students’ association points to RTI data, says only 2 seats filled by SC and 4 by OBC candidates against sanctioned strength of 18 and 34 respectively

The Foundation for Excellence Scholarship for Technical Courses 2024-25 programme provides financial assistance to meritorious students from economically disadvantaged backgrounds who aspire to pursue undergraduate degrees in engineering, medicine or law.

Western Digital is offering a one-time scholarship of up to Rs 75,000 to persons with disabilities and transgender students pursuing graduation, post-graduation and PhD degrees in STEM-related fields.

A Competition Commission probe has found that food delivery platforms Zomato and Swiggy indulged in unfair business practices, including alleged preferential treatment to some restaurant partners, according to sources. The detailed investigation was ordered by the Competition Commission of India (CCI) in April 2022 and the probe report was submitted to the regulator earlier this year. Under the norms, CCI director general's report has been shared with the parties concerned and later, they will be called for hearings by the watchdog.

The Foundation for Excellence Scholarship for Technical Courses 2024-25 programme provides financial assistance to meritorious students from economically disadvantaged backgrounds who aspire to pursue undergraduate degrees in engineering, medicine or law.

Western Digital is offering a one-time scholarship of up to Rs 75,000 to persons with disabilities and transgender students pursuing graduation, post-graduation and PhD degrees in STEM-related fields.

Like man buns on scooters and ukulele busking, Covid-19 has now spread to the north from London – inspiring a coronavirus soapcom from our self-isolating comedy-writer

Up here in the north-west, we’re used to living in the slipstream of London’s sleek urban shenanigans. Whatever the cultural breakthrough – man buns on scooters, cashless ukulele busking, emotional support bees – it takes a while to reach the Lancaster and Morecambe Non-Metropolitan Area. If it ever does.

A Street Stranger Watch leads to a death and the appearance at midnight of the street’s original Victorian inhabitants

25 April 1985: The new taste is said to be smoother, rounder and bolder, not to mention more harmonious

The hard-nosed men of the New York Stock Exchange yesterday made a snap judgment on the most sensational news in the mighty American soft drinks industry for 99 years. Without even trying the new, sweeter formula Coca-Cola, they backed the initial shock reaction of the amateurs: it tastes more like Pepsi.

By lunchtime yesterday Coca-Cola shares had taken another 1.50 cent pounding on top of the 1.60 they sustained in late trading after the new formula was officially unveiled on Monday, despite the assurances of Coke’s chairman, Mr Roberto Goizueta, that the new taste is smoother, rounder and bolder, not to mention more harmonious. Reporters disagreed.

Related: Milk Coke: another classic from the nation that invented Cheeky Vimto

From tweet to tweet, official to official, nobody can tell what his priorities are

England stars Maro Itoje and Mako Vunipola are yet to commit to playing for Saracens in the Championship next season. Two French clubs have already expressed interest in Itoje.

EXCLUSIVE: Billy Vunipola has played a supporting role in rugby's version of House of Cards. The No 8 turned political pawn as his name was touted in promises for World Rugby elections

Jamie Laing has appeared to flout lockdown rules after he returned to London with his girlfriend Sophie Habboo. The Made in Chelsea couple had left the capital to isolate in the Cotswolds.

Benchmark indices ended the week lower, with a near 6.3 per cent fall on the Nifty. The drop came largely on the back of a severe fall at the start of the week, after which the markets could not recoup those

Benchmark indices ended the week lower, with a near 6.3 per cent fall on the Nifty. The drop came largely on the back of a severe fall at the start of the week, after which the markets could not recoup those

Investors with a medium-term perspective can sell the stock of Kansai Nerolac Paints at current levels.The stock had encountered a key resistance in t

1. NRL 2020: Erin Molan condemns ‘incredibly dangerous’ Holocaust Instagram post, Bryce Cartwright wife  NEWS.com.au
2. Gold Coast Titans anti-vaccination: Doctor’s warning over flu jab after NRL players stood down  Gold Coast Bulletin
3. NRL 2020, news: Bryce Cartwright’s wife hits out in flu shot storm, Instagram post, anti-vaccination players react  NEWS.com.au
4. NRL: Anti-vaxxers stood down as vaccination debate rages on  news.com.au
5. 'It's a team sport boys': Cronulla skipper Wade Graham calls out anti-vaxxers  Wide World of Sports
6. View Full coverage on Google News

Fauci, 79, told CNN correspondent Jake Tapper that the contact was “low risk" — meaning he did not have direct contact with the sick staffer. A test Friday found Fauci did not have COVID-19, CNN reported.

Fauci, 79, told CNN correspondent Jake Tapper that the contact was “low risk" — meaning he did not have direct contact with the sick staffer. A test Friday found Fauci did not have COVID-19, CNN reported.

Metropolitan Police Federation chairman says planned 14-day strategy is unenforceable

Dynamo Dresden, who play in the second tier of German Football, have put their entire squad and coaching staff into two-week isolation after recording two new coronavirus cases.

Google Poland Logo - Stanisław Lem

Dynamo Dresden, who play in the second tier of German Football, have put their entire squad and coaching staff into two-week isolation after recording two new coronavirus cases.

A polar vortex has blasted into the northeastern US this weekend bringing rare May snowfall and record low temperatures to some areas.

Members of the Joint Operational Committee in Tshwane is in self-isolation after one member tested positive for Covid-19, spokesperson Brigadier Vishnu Naidoo has told News24.

French tennis chief Bernard Guidicelli admitted that Roland Garros could be staged behind closed doors.

Many in the region had to dust off their snow shovels for at least one more dig out on Saturday morning after a polar vortex blasted parts of the province, catching many off guard.

The following article, Witness Tampering? Asst. HHS Secretary Releases Threatening Text Messages From Dem Rep. Eric Swalwell: “In clear violation of House Ethics rules “, was first published on 100PercentFedUp.com.

Now that Michael Caputo, who was previously a target of the Mueller investigation, has been cleared, he has released some pretty damning text messages from the virulently anti-Trump lawmaker from California, Rep. Eric Swalwell (D). The text messages appear to prove that Swalwell, who has spent the last 3 1/2 years calling for Trump’s impeachment, […]

Continue reading: Witness Tampering? Asst. HHS Secretary Releases Threatening Text Messages From Dem Rep. Eric Swalwell: “In clear violation of House Ethics rules “ ...

Research Event

Event participants

Dr Maria Ângela Bragança, Secretary of State for Cooperation, Ministry of External Relations, Angola

12 March 2015 , Volume 91, Number 2

Hidden in the flash. posted a photo:

With a lockdown in place it is against the rules for me to go to places I like to shoot, so I though I would try to create a series called Isolated creativity. The series is not intended to be a diary but a way of documenting thoughts and emotions via photography.

I've felt a bit like Pink Rabbit over the passed few day. I'm not fed up and depressed by the lockdown but by the people who think that it's okay to break the rules. By the tabloid media that run stories that convince people it's okay to go out and about, when it Isn't. By the political points scoring that has started to appear in all forms of media.Lastly I fed up with second home owners that have turned up during lockdown and appear to be going out and about most days.

Just like Pink Rabbit I have the blues.

Protease-activated receptor 2 (PAR-2) is activated by secreted proteases from immune cells or fungi. PAR-2 is normally expressed basolaterally in differentiated nasal ciliated cells. We hypothesized that epithelial remodeling during diseases characterized by cilial loss and squamous metaplasia may alter PAR-2 polarization. Here, using a fluorescent arrestin assay, we confirmed that the common fungal airway pathogen Aspergillus fumigatus activates heterologously-expressed PAR-2. Endogenous PAR-2 activation in submerged airway RPMI 2650 or NCI–H520 squamous cells increased intracellular calcium levels and granulocyte macrophage–colony-stimulating factor, tumor necrosis factor α, and interleukin (IL)-6 secretion. RPMI 2650 cells cultured at an air–liquid interface (ALI) responded to apically or basolaterally applied PAR-2 agonists. However, well-differentiated primary nasal epithelial ALIs responded only to basolateral PAR-2 stimulation, indicated by calcium elevation, increased cilia beat frequency, and increased fluid and cytokine secretion. We exposed primary cells to disease-related modifiers that alter epithelial morphology, including IL-13, cigarette smoke condensate, and retinoic acid deficiency, at concentrations and times that altered epithelial morphology without causing breakdown of the epithelial barrier to model early disease states. These altered primary cultures responded to both apical and basolateral PAR-2 stimulation. Imaging nasal polyps and control middle turbinate explants, we found that nasal polyps, but not turbinates, exhibit apical calcium responses to PAR-2 stimulation. However, isolated ciliated cells from both polyps and turbinates maintained basolateral PAR-2 polarization, suggesting that the calcium responses originated from nonciliated cells. Altered PAR-2 polarization in disease-remodeled epithelia may enhance apical responses and increase sensitivity to inhaled proteases.

The dextransucrase DSR-OK from the Gram-positive bacterium Oenococcus kitaharae DSM17330 produces a dextran of the highest molar mass reported to date (∼109 g/mol). In this study, we selected a recombinant form, DSR-OKΔ1, to identify molecular determinants involved in the sugar polymerization mechanism and that confer its ability to produce a very-high-molar-mass polymer. In domain V of DSR-OK, we identified seven putative sugar-binding pockets characteristic of glycoside hydrolase 70 (GH70) glucansucrases that are known to be involved in glucan binding. We investigated their role in polymer synthesis through several approaches, including monitoring of dextran synthesis, affinity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzymes. Substitution of only two stacking aromatic residues in two consecutive sugar-binding pockets (variant DSR-OKΔ1-Y1162A-F1228A) induced quasi-complete loss of very-high-molar-mass dextran synthesis, resulting in production of only 10–13 kg/mol polymers. Moreover, the double mutation completely switched the semiprocessive mode of DSR-OKΔ1 toward a distributive one, highlighting the strong influence of these pockets on enzyme processivity. Finally, the position of each pocket relative to the active site also appeared to be important for polymer elongation. We propose that sugar-binding pockets spatially closer to the catalytic domain play a major role in the control of processivity. A deep structural characterization, if possible with large-molar-mass sugar ligands, would allow confirming this hypothesis.

Bacterial biofilms are cellular communities that produce an adherent matrix. Exopolysaccharides are key structural components of this matrix and are required for the assembly and architecture of biofilms produced by a wide variety of microorganisms. The human bacterial pathogens Escherichia coli and Salmonella enterica produce a biofilm matrix composed primarily of the exopolysaccharide phosphoethanolamine (pEtN) cellulose. Once thought to be composed of only underivatized cellulose, the pEtN modification present in these matrices has been implicated in the overall architecture and integrity of the biofilm. However, an understanding of the mechanism underlying pEtN derivatization of the cellulose exopolysaccharide remains elusive. The bacterial cellulose synthase subunit G (BcsG) is a predicted inner membrane–localized metalloenzyme that has been proposed to catalyze the transfer of the pEtN group from membrane phospholipids to cellulose. Here we present evidence that the C-terminal domain of BcsG from E. coli (EcBcsGΔN) functions as a phosphoethanolamine transferase in vitro with substrate preference for cellulosic materials. Structural characterization of EcBcsGΔN revealed that it belongs to the alkaline phosphatase superfamily, contains a Zn2+ ion at its active center, and is structurally similar to characterized enzymes that confer colistin resistance in Gram-negative bacteria. Informed by our structural studies, we present a functional complementation experiment in E. coli AR3110, indicating that the activity of the BcsG C-terminal domain is essential for integrity of the pellicular biofilm. Furthermore, our results established a similar but distinct active-site architecture and catalytic mechanism shared between BcsG and the colistin resistance enzymes.

OM Arts artists participating in the Incarnate programme in Italy create a collaborative piece of prophetic art for the local community.

When Theresa May declared "Brexit means Brexit," Nicola Sturgeon's response was pithy and to the point. "Remain means Remain," she said, making an apparently all-or-nothing commitment to securing Scotland's place in the EU after the country voted decisively to stay.