The 4,500-year-old pyramids of Giza are the backdrop for "Forever Is Now," which features sculptures, installations and immersive artworks that explore the relationship between the past and present

The terms “snake oil” and “snake-oil salesperson” are part of the vernacular thanks to Clark Stanley, a quack doctor who marketed a product for joint pain in the late 19th century

Laboratory study of natural populations of mosquitoes can play a key role in determining the underlying causes of variation in burdens of mosquito-borne disease. Aedes aegypti is the main vector of the viruses that cause dengue, chikungunya, Zika, and yellow fever, making it a high priority for laboratory study. Ae. aegypti eggs provide an ideal starting point for new laboratory colonies. Eggs can be collected using ovicups, which are small plastic cups lined with seed-germination paper and partially filled with leaf-infused H₂O. Once collected, dry eggs will remain viable for months and can be safely transported long distances back to the laboratory as long as they are properly stored. This protocol provides step-by-step instructions for preparing for collecting, storing, and hatching Ae. aegypti eggs and has successfully yielded laboratory colonies from locations across both the native and invasive range of this species.

Creating transgenic mosquitoes allows for mechanistic studies of basic mosquito biology and the development of novel vector control strategies. CRISPR–Cas9 gene editing has revolutionized gene editing, including in mosquitoes. This protocol details part of the gene editing process of Aedes aegypti mosquitoes via CRISPR–Cas9, through testing and validating single-guide RNAs (sgRNAs). Gene editing activity varies depending on the sequence of sgRNAs used, so validation of sgRNA activity should be done before large-scale generation of mutants or transgenics. sgRNA is designed using online tools and synthesized in <1 h. Once mutants or transgenics are generated via embryo microinjection, sgRNA activity is validated by quick genotyping polymerase chain reaction (PCR) and DNA sequencing.

The insect eggshell is a multifunctional structure with several important roles, including generating an entry point for sperm via the micropyle before oviposition, serving as an oviposition substrate attachment surface, and functioning as a protective layer during embryo development. Eggshell proteins play major roles in eggshell tanning and hardening following oviposition and provide molecular cues that define dorsal–ventral axis formation. Precise eggshell formation during ovarian follicle maturation is critical for normal embryo development and the synthesis of a defective eggshell often gives rise to inviable embryos. Therefore, simple and accurate methods for identifying eggshell proteins will facilitate our understanding of the molecular pathways regulating eggshell formation and the mechanisms underlying normal embryo development. This protocol describes how to isolate and enrich eggshells from mature oocytes of Aedes aegypti mosquitoes and how to extract their eggshell proteins for liquid chromatography with tandem mass spectrometry (LC–MS/MS) proteomic analysis. Although this methodology was developed for studying mosquito eggshells, it may be applicable to eggs from a variety of insects. Mosquitoes are ideal model organisms for this study as their ovarian follicle development and eggshell formation are meticulously regulated by blood feeding and their follicles develop synchronously throughout oogenesis in a time-dependent manner.

In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner.

The union for wildland firefighters says a recently passed Ontario bill that makes them eligible for presumptive cancer care under the Workplace Safety and Insurance Board doesn't go far enough.

The crowning achievement at last year's COP climate conference in Dubai was a global consensus on the need to "transition away from fossil fuels." This year, the conference had not even started yet before BBC News exposed senior organizers of using the event to arrange potential deals for fossil fuel expansion. The recent election of Donald Trump in the U.S. has added an additional layer of uncertainty to this year's meeting.

The National Capital Commission says it opened the Rideau Canal Skateway below its usual stands for ice thickness a few times last season — and plans to do the same this year. 

Mozaik church, pioneered by the OM team in Bar, Montenegro, has recently baptised seven new believers in the Adriatic sea, at the beach.

Company Renowned for ‘Headset’ Innovation Cuts Time and Cost with SolidWorks Design and Simulation Software

Get all the latest updates on COMEX Copper options including a new all-time high trading record and the strong growth of RFQs.

Traders have discovered the benefits of coordinating option expirations with the release of economic data focusing on the impact of certain indicators.

A newly-discovered iPhone 14 Pro prototype features one of the earliest known incarnations of Apple's scrapped haptic button project.

OM Hungary’s baseball ministry celebrates brothers in Christ and starts new family outreach.

A public baptism ceremony at the river becomes an opportunity to teach an onlooker about the true meaning of baptism.

Cycling to reach people in remote villages in Malawi, Yolanda Mamvura experienced a puncture which led her to pray for an epileptic.

OM Malawi endeavours to make its projects and workers self-sustainable, while transforming lives and communities at the same time.

A News24 Business reader has to pay R500 to receive a debt clearance certificate but is currently unemployed.

A News24 reader looks to an expert for guidance on the best investment vehicles for each of her children.

At the heart of missions lies a passion for justice. In every community in which OM is involved, working for justice for the oppressed and vulnerable is part of ministry.

Republicans have some big questions for the U.S. Department of Education's plans to begin a 50-state strategy to address the unequal distribution of top teachers.

The cryptocurrency climbed as much as 4.3% to an unprecedented $79 771 on Sunday.

When it comes to parenting students with learning differences, every family's experience is unique. And that reality has never been more true than it is now as millions of students are out of school due to the coronavirus pandemic.

Penn State Health will collect unwanted, unneeded or expired medications, needles and syringes for safe disposal on Saturday, Oct. 26, as part of National Prescription Drug Take-Back Day.

Penn State World Campus will accept credits from certain courses offered by the Community College of the Air Force that can be transferred toward a bachelor of science in organizational leadership. The degree is offered online through Penn State World Campus by the School of Labor and Employment Relations in the College of the Liberal Arts.

This week SA Money Report tries to understand eThekwini's surprising plan for nuclear power, and whether the ANC's RET faction has a hand in things.

The Penn State Center for Human Evolution and Diversity is currently accepting proposals for grants to support projects occurring during the 2024-25 academic year.

When creating bar charts, it is very common to display labels with the bars to make it easier to determine the bar values or to provide additional information in the chart. However, these labels can take away valuable data space, particularly if you generate a smaller-sized graph. As you see [...]

The post Optimizing bar label placement appeared first on Graphically Speaking.

Dressed as clowns and carrying posters, students of OM Brazil’s missions training protested against corruption in São José dos Campos on 23 March.

WILMINGTON – Governor John Carney and the Delaware Children’s Department have joined the federal ALL-IN Foster Adoption Challenge, raising awareness of permanency efforts for children and youth in foster care. This challenge, spearheaded by the Administration for Children and Families, raises awareness and looks to build partnerships that focus on the goal of finding loving, permanent […]

WILMINGTON – Delaware officials, families, friends and community partners joined together on National Adoption Day to recognize the resilience of families who have embarked on the journey of adoption in the past year. Hosted by the Interagency Committee on Adoption, which is a coalition of adoption organizations and stakeholders from across the state, the annual National […]

WILMINGTON – For the second year in a row, Delaware officials, families, friends and community partners joined together virtually on National Adoption Day to recognize the resilience of families who have embarked on the journey of adoption in the past year. The National Adoption Day event on Saturday, November 20, hosted by the statewide Interagency Committee […]

Annual event honors families adopting and family service workers DOVER – On November 18, the State of Delaware came together to celebrate National Adoption Day, a day dedicated to highlighting the importance of adoption and the support available to adoptive families. This special program, hosted by the Interagency Committee on Adoption, a collaboration between Delaware’s […]

Saudi Arabia announced that it was replacing the chief executive of its futuristic mega-city NEOM as scepticism mounts over the Gulf kingdom's most ambitious development projects.

Kyle Singler's videos have left the NBA fraternity concerned with many of his former teammates offering support.

The chilling video shows a masked individual crossing the street and approaching a father walking hand-in-hand with two children.

At least three airlines cancelled flights between Australia and Bali this week after a volcano eruption in eastern Indonesia spewed a vast plume of volcanic ash into the air.

Recent DNA findings from Pompeii reveal complex ancestry and social diversity, challenging earlier assumptions about family structures and cultural roles in the ancient city. Analysing skeletal remains has shown that some groups presumed to be family members were not biologically related, while traditional gender roles were also re-evaluated. This research highlights the cosmopolitan nature of Pompeii’s society and opens a new chapter in understanding life before the eruption of Mount Vesuvius.

Android 15 was released globally in August and since then, original equipment manufacturers (OEMs) such as OnePlus, Oppo and Realme have introduced their iterations of the new operating system (OS) for their smartphones and other devices. The update is said to bring support for true adaptive refresh rate (ARR) which leverages discrete VSync steps to adapt to the frame rate of the on-screen content without changing the current display mode.

Realme is reportedly gearing up to unveil the Realme 14 Pro Lite in India as its model number, RAM, storage variants and colour options have surfaced online. The upcoming model is believed to be part of Realm's 14 series. The Realme 14 Pro Lite is said to come with up to 12GB of RAM and a maximum of 512GB of onboard storage. It could go official alongside the Realme 14 and Realme 14 Pro.

Vivo Y300 Plus with Snapdragon 695 SoC was launched in India last month. According to a report, Vivo is also readying to launch the Y300 in the country. Colourways and specifications of the upcoming Vivo Y series phone have also leaked alongside its India launch timeline. It is said to be available in three colour options. The Vivo Y300 could boast a Sony IMX882 portrait camera.